Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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104 kb

RECOMMENDATIONS FOR THE INSTALLATION OF PROTECTIVE PLANTINGS ALONG ROADS (SNOW BREAKAGE)

abstract 1451901011 issue 145 pp. 1 – 6 31.01.2019 ru 56
The significant part of our country's roads is subject to snowdrifts in winter. To ensure smooth and uninterrupted traffic in road transport during winter time, road facilities have to use vast amounts of mechanized and manual labor, as well as spend large sums of money. There are cases of snowdrifts on roads and interruptions in the traffic of road transport causing serious damage to the national economy of the country. Road workers face an important task of ensuring uninterrupted traffic in road transport in winter and reduce the cost of the road maintenance. In this regard, road facilities use a variety of means in order to protect motor roads from snow drifts: installation of portable shields, permanent fences, snow-protective plantings of various widths and structures. The evidence from practice shows that forest stand is the most durable, reliable and economical means of protecting roads from snowdrifts (snow breaking tree belts)
258 kb

HYGROSCOPICITY OF MATURE GRAIN AS A MARKER OF WINTER BARLEY AND SUNFLOWER FROST RESISTANCE

abstract 1451901012 issue 145 pp. 7 – 30 31.01.2019 ru 65
In the review article we present a comparative analysis of frost resistance of varieties of winter barley and sunflower (Helianthus annuus L.) by the results of freezing in the refrigerating chambers and by the hygroscopicity of mature grain. On the example of a number of varieties of both cultures, it is shown that the higher the frost resistance of the variety, the smaller the volume of the supernatant can be obtained by extracting the press cake with a solution containing magnesium cations. It is assumed that increased hygroscopicity of winter barley and sunflower press cake is associated with a relatively high content of so - called "water-soluble starch" - β-glucan polysaccharides in its grain. The possibility of regulation of the degree of hygroscopicity under the influence of potassium (KNO3), zinc (ZnSO4) and gibberellic acid (GA3) was studied on a number of sunflower varieties. It was shown that treatment with a solution of potassium (a concentration of 50 ppm KNO3) of sunflower plants at the stage of 4 leaves leads to a significant increase in hygroscopicity of grain and hardiness of plants, little effect was given treatment with zinc (30 ppm ZnSO4) and a lesser effect was observed when treating plants gibberellovaja acid (20 ppm). Potassium treatment significantly intensified the biosynthesis of free Proline, phenolic compounds and soluble proteins. When processing zinc significantly increased the content of carbohydrates in sunflower plants. Hygroscopicity showed a significant correlation with the content of free Proline (R2=0.621), phenolic compounds (R2=0.907), total carbohydrates (R2=0.673) and soluble proteins (R2=0.708). It is assumed that the treatment of plants with potassium and zinc plays a key role in increasing the hygroscopicity of grain. The results of comparative studies of frost resistance on the degree of survival of plants during freezing in cold rooms and the degree of hygroscopicity of mature grain showed that these two methods of assessing frost resistance give very close data. At the same time, in terms of simplicity and low economic costs, the proposed method of assessing frost resistance is many times superior to the method of direct freezing of plants
11340 kb

The synthesis of the semantic nuclei of scientific specialties of the higher attestation commission of the russian federation and the automatic classifica-tion of articles according to scientific fields with the use of asc-analysis and "eidos" intellectual systems (on the example of scientific journal of kuban state agrarian university and its scientific specialties: mechanization, agronomy and veterinary medicine)

abstract 1451901033 issue 145 pp. 31 – 102 31.01.2019 ru 57
14 January 2019 at the website of the higher attestation Commission of the Russian Federation http://vak.ed.gov.ru/87 the information appeared: "About refining of scientific specialties and their respective fields of science where publications are included in the List of peer-reviewed scientific publications, where basic scientific results of dissertations on competition of a scientific degree of candidate of Sciences, on competition of a scientific degree of the doctor of Sciences must be published ". It is reported that according to the recommendation of the HAC for other publications included in the List of groups of scientific specialties, the work on refining scientific specialties and branches of science will be continued in 2019. This work is a continuation of the author's series of works on cognitive linguistics. It offers innovative intelligent technology to automate the solution of the problem formulated by the higher attestation Commission of the Russian Federation above. With the use of the automated system-cognitive analysis (ASC-analysis) and its programmatic toolkit which is intellectual system called "Eidos" directly on the basis of official texts of passports of scientific specialties of the higher attestation Commission of the Russian Federation, there were established their semantic kernels, and then, implemented the automatic classification of scientific texts (articles, monographs, textbooks, etc.) on the specialties and groups of specialties of the higher attestation Commission of the Russian Federation. Traditionally, this task is solved by dissertation councils, as well as editorial boards of scientific publications, i.e. by experts, on the basis of expert assessments, in an informal way, on the basis of experience, intuition and professional competence. However, the traditional approach has a number of serious drawbacks that impose significant limitations on the quality and volume of analysis. Therefore, the efforts of researchers and developers to overcome these limitations are relevant. Currently, there are all grounds to consider these restrictions as unacceptable, because they are not only necessary, but also quite possible to overcome. Thus, there is a problem, the solution of which is the subject of consideration in this article. A detailed numerical example of solving the problem on real data is given as well
426 kb

CHANGE OF INDICATORS OF FERTILITY OF BLACK SOIL UNDER DIFFERENT TECHNOLOGIES OF CULTIVATION OF MAIZE IN TERMS OF SEED FARMING

abstract 1441810011 issue 144 pp. 1 – 18 28.12.2018 ru 49
The article presents the results of a study to determine the changes in fertility in the typical black soil of the Belgorod region with different technologies of maize cultivation in seed farming
157 kb

BIOLOGICAL CONTROL OF THE APPLE MOTH NUMBER BASED ON ENTOMOPATHOGENIC VIRUSES (REVIEW)

abstract 1441810012 issue 144 pp. 19 – 31 28.12.2018 ru 17
The article considers the data on the applicability of bioinsecticides based on the granulovirus as an active ingredient against the apple moth. The methods for producing strains of the Cydia pomonella granulovirus to develop bioinsecticides are explained. The methods for the reproduction of laboratory populations of insects, the method of their infection and isolation of viral onset are described. The stages of obtaining bioinsecticides in vitro, as well as methods for their storage are provided
222 kb

DEVELOPMENT OF MULTIPLEX SETS OF SSR MARKERS FOR GENOTYPING APRICOT VARIETIES (PRUNUS ARMENIACA L.)

abstract 1441810013 issue 144 pp. 32 – 43 28.12.2018 ru 16
Genetic studies of apricot are the actual direction in the genetics of fruit crops. In this regard, the improvement of the collection of SSR markers for the genotyping of this culture is an objectively significant task. In a study for the 16 SSR-markers previously developed on almonds (PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1, PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1) and Siberian apricot (A3-72, A1-63, H2-22, A3- 7-1, H2-5, A1-7, A3-9, H2-45), approbation and evaluation of the prospects of using for genotyping Prunus armeniaca L. were performed. Approbation, performed on 3 varieties of different origin, revealed markers and their combinations optimal for their use. During the study, all tested DNA markers were grouped into multiplex sets, including 4 markers. This allows carrying out genotyping simultaneously on 4 loci in the formulation of one reaction. One marker (PdUnchar2) from the studied sample included in the multiplex set did not show amplification. Five markers gave a monomorphic product. The remaining 11 SSR markers allowed us to obtain polymorphic, cultivar-specific SSR fingerprints for all the studied cultivar. These multiplex sets are proposed for use in studying the genetic polymorphism of the species Prunus armeniaca L.
14224 kb

FORMATION OF A SEMANTIC KERNEL IN VETERINARY MEDICINE WITH THE AUTOMATED SYSTEM-COGNITIVE ANALYSIS OF PASSPORTS OF SCIENTIFIC SPECIALTIES OF THE HIGHER ATTESTATION COMMISSION OF THE RUSSIAN FEDERATION AND THE AUTOMATIC CLASSIFICATION OF TEXTS ACCORDING TO THE AREAS OF SCIENCE

abstract 1441810033 issue 144 pp. 44 – 102 28.12.2018 ru 28
This work is a continuation of the author's series of works on cognitive veterinary medicine. The present period is characterized by the appearance of huge volumes of texts in different languages in the open access, generated by people. Currently, these texts are accumulated in various electronic libraries and bibliographic databases (WoS, Scopus, RSCI, etc.), as well as on the Internet on various sites. All these texts have specific authors, dates and can belong simultaneously to many non-alternative categories and genres, in particular: educational; scientific; artistic; political; news; chats; forums and many others. The solution of the generalized problem of attribution of texts is of great scientific and practical interest, i.e. studying these texts, which would reveal their probable authors, date of creation, the ownership of these texts to the above generalized categories or genres, and might evaluate the similarities - differences of authors and texts according to their content, highlight key words etc. To solve all these problems it seems necessary to form the generalized linguistic images of texts into groups (classes), i.e. to form semantic kernels of classes. A special case of this problem is the creation of the semantic kernel in various scientific specialties of the HAC of the Russian Federation and the automatic classification of scientific texts in the areas of science. Traditionally, this task is solved by dissertation councils, i.e. experts, on the basis of expert assessments, i.e. in an informal way, on the basis of experience, intuition and professional competence. However, the traditional approach has a number of serious drawbacks that impose significant limitations on the quality and volume of analysis. Currently, there are all grounds to consider these restrictions as unacceptable, because they can be overcome. Thus, there is a problem, the solutions of which are the subject of consideration in this article. Therefore, the efforts of researchers and developers to overcome them are relevant. Therefore, the aim of the work is to develop an automated technology (method and tools), as well as methods of their application for the formation of the semantic core of veterinary medicine by automated system-cognitive analysis of passports of scientific specialties of the HAC of the Russian Federation and automatic classification of texts in the areas of science. A detailed numerical example of solving the problem on real data has been given as well
188 kb

SEED PRODUCTION AND TECHNOLOGICAL METHODS OF PRODUCTION OF SUGAR BEET SEEDS IN THE KRASNODAR REGION

abstract 1431809011 issue 143 pp. 1 – 15 30.11.2018 ru 151
The article discusses the organizational scheme of sugar beet seed production, as well as technological methods of seed production using the stick method and the cultivation of basic seeds in NRU. We substantiate the key elements of the cultivation of uterine sugar beet of summer sowing season
429 kb

A MODIFIED PROTOCOL OF RNA ISOLATION FROM MATURE LEAVES OF GRAPES FOR RT-PCR

abstract 1431809012 issue 143 pp. 16 – 30 30.11.2018 ru 148
Isolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
162 kb

CLONALE MICROPROPOGATION AND SANITATION OF PRUNUS DOMESTICA FROM THE PLUM POX POTYVIRUS (PPV)

abstract 1431809013 issue 143 pp. 31 – 40 30.11.2018 ru 210
In the article we present the analysis of the results of studies of russian, foreign scientists, as well as our own data, obtained in the clonal micro-multiplication and sanitation of the plum domestic from Sharkey's virus (PPV). The urgency of work is determined by the fact that the plum is the second on the significance culture in horticulture of the south of Russia. One of the most economically significant objects of virus etiology on the discharge of relative is considered to be Sharkey's virus of plum (Plum pox potyvirus). In the Krasnodar region and in the Russian Federation for the first time, Sharkey's virus of plum was discovered in the end of the 1980th, after which it widely extended into other regions of the country. In the complex of the methods of sanitation from Sharkey's virus of plum we successfully use thermo- and chemotherapy in combination with the method of apical meristem. The mechanism of the formation of virus-free meristem consists of the delay of the process of the replication of virus particles from the rapid, anticipating increase in the rudimentary cloths and organs, especially, if plant undergoes by thermo- or chemotherapy. As virucide there are used the preparations neo -DHt (85 mG/l), and also salycilic acid in the concentration of 3kh10-4 M with the simultaneous magnetic-pulse working of mericlons. As the most suitable nutrient medium for the micro-multiplication of plum we considered Murasige- Skoog medium, on basis of which we have prepared different modifications. As the growth factors, which increase the effectiveness of multiplication, we used 6-BAP in the concentration 0,5-1 mG/l (in the stage of introduction into the culture and the stage of animated cartoon). For the improvement in the quality of the micro-shoots of plum, additionally to the growth factors, one should apply succinic acid, succinates of potassium and sodium in the concentration 4 mG/l. Obtained in the course of clonal micro-multiplication and adapted mericlons will be tested to the virus carrying ability and will be approved according to the quality signs. The ovaries of initial plants are embedded by healthy quality seedlings
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