This overview and theoretical article deals with the consideration of hypothetical possibilities for the development of molecular-kinetic markers of agricultural plants allowing to quantify the effect of genotype-environment interaction on the basis of stability studies of mRNA. The development in view is based on the results of studies of the mRNA decay identity in vivo and in vitro (the ommp system), as well as the phenomenon of RNA interference (RNAi), widely studied in plants. The ommp system has allowed to establish the relationship of the cultivar-specific growth reaction to the effects of low positive temperatures, dehydration, salinity, illumination and biologically active substances with the stability of the total and a number of gene-specific mRNAs of green and etiolated seedlings of winter soft wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.). Similar studies of mRNA stability have been conducted on ripening grains of maize (Zea mays L.), with a particular result of the in vivo and in vitro decay identity confirmed for major mRNAs of stored proteins, 19 and 22 kDa zeins, of normal maize and mutant according to the regulatory gene opaque-2, changing the amount and stability of zein mRNA in the maturing grain of high-lysine maize. Regulatory response of the organism through RNAi is also multiple and includes neutralization of viral and bacterial infections, reaction to pathogens and biologically active substances, circadian rhythms, water stress, hypoxia, mechanical stress, mineral nutrition, salt stress and temperature changes. Unfavorable environmental influences result in an increase or decrease in the expression of certain microRNAs (miRNAs). The change in mRNA stability is an important component of the gene expression regulation system in eukaryotic cells. The main determinants of mRNA stability are in the 3'-untranslated region. It is the It is the (U)nА sequence and the degree of polyadenylation of mRNA, i.e. length of its terminal homonucleotide chain. It is to this region that mRNA molecules are complementary to miRNA. The most important component, which largely determines the regularity of the genotype-environment interaction, is the polyadenyl sequence at the 3 'end of the mRNA. Its length depends both on the genotype and on the environmental conditions. There is evidence that polyadenylation degree of mRNA determines the secondary structure of the molecule. As is known, deadenylation of mRNA reduces its lifetime, and when the poly-A-tail reaches several dozens of nucleotides, an explosive disintegration of the mRNA molecule occurs. Consequently, in a living cell the following mRNA decomposition scheme appears to be logical: shortening the poly-A-tail of the mRNA opens the sites of miRNA interaction with the 3'-non-coding region of the mRNA molecule, which causes its degradation. Thus, it is safe to assume that the interaction of mRNA and miRNA in the ommp system is the underlying process for molecular-kinetic markers under development
The article considers phenomenon of the formation of giant fruits in higher plants. As regards the size of plants, several positions are distinguished such as giant fruit, in a common habit, i.e. the size of the whole plant; depending on the harvest. Examples of different types of gigantism in plants are given. It is shown that since the ancient times the phenomenon of gigantism has been widespread. Various reasons for the appearance of abnormally large plants and fruits are considered, growth genes regulating the process of cell division and formation are given. The study of the connections between plant organs that conduct and consume the assimilant, redistribute and use carbon is easy in the concept of donor-acceptor relationships. As a model object for studying the growth of giant fruits, we have examined varieties of pumpkin called Cucurbita maxima. Thus, in a short period, a paradigm shift occurred. From surprise and enthusiasm for the gigantism of plants, the issue of cellular growth regulation, the criteria for evaluating the reproduction process, the search for links with the size, number of cells and their properties is become. New model objects have appeared, the analysis of images of plant giants has shown a unique visual resource on various objects. New knowledge about the growth of plants and the ability to regulate the process formed the basis for selection programs for obtaining vegetables with specified fruit parameters, which makes them competitive in the market nowadays
The article considers data obtained in long-term stationary experiment in Kuban state agrarian University, which was started in 1991 for old irrigated leached black soil of the central zone of the Krasnodar region. Studies were conducted in two rotations of seven-field grassy-grain-row crop rotation. The duration of irrigation - more than 30 years. It is established that long-term irrigation by sprinkling led to the soil over-compaction in both arable and subsurface layers. In the arable layer of 0-30 cm density of 0.14-0.17 g/cm3 exceeds the maximum allowable. We have noticed a sharp decrease in humus in the arable layer to 2.46-2.67 per cent. The weak acidification of the active root layer was noted, salt PH 5.0-5.3. In the soil-absorbing complex, the content of calcium decreases. It was found that the productivity of crops cultivated in the rotation of two rotations was greatly influenced by the system of basic soil treatment. In the first rotation, the decrease in productivity of cultivated crops against the background of the surface treatment system amounted to an average of 4.2% compared with deep: subsurface tillage and moldboard. In the second rotation after 14 years against the background of shallow surface treatment crop rotation productivity decreased by 11.2 %. The dependence of the fertilizer system and crop yield on the background of different main soil tillage is established. When using the mineral fertilizer system, the decrease in crop productivity, while minimizing the main tillage in the second rotation was 15.2%, and against the background of the organic fertilizer system by 12.7%. The deep sub-soil treatment provided an increase in the productivity of old irrigated leached black soil in the second rotation against organic fertilizers by 3.6%.
Thus, the organic system of fertilizers on the background of deep main soil tillage allows to obtain crop yields in the framework of grassy-grain-row crop rotation on wetlands degraded old-irrigated lands is not lower than using high doses of mineral fertilizers
Coloring the plumage of chickens is a complex genetically determined sign. The study of the interactions of the main genes of chicken pigmentation was started by A.S. Serebrovsky. The most complete information on the genes of coloring plumage of chickens and down of chickens is given by Z.M. Kogan, indicating the clutch groups and the arrangement of these genes on the chromosome map. In the 1990s, the interaction of color genes, pen pattern and modifier genes affecting the intensity of the main color and pattern of the pen was described in detail. It was found that in pigs the pigmentation is regulated by the MC1-R melanocortin receptor associated with the locus E. Due to molecular studies, loci of dominant white color (I), recessive white color (c / c), striped pen pattern (B) adherent to the floor , and spotted spot locus (mo). The final phenotype depends not only on individual genes, but also on their interactions and the genetic environment as a whole. The same genes can produce different patterns and shades of the main plumage, and different genes determine the same phenotypes. Despite the fact that there was significant progress in determining the molecular causes of the appearance of a particular type of color in chickens, much remains to be learned
This article briefly discusses a new innovation (brought to a level that ensures its practical use) method of artificial intelligence: automated system-cognitive analysis (ASC-analysis) and its programmatic toolkit which is called intellectual system "Eidos". A detailed numerical example of the solution demonstrating the technology of creating a veterinary diagnostic test of gastrointestinal diseases of horses is given. As the source data, we use data from the UCI repository, kindly given by Mary McLeish and Matt Cecile (Department of computer science of University of Guelph, Ontario, Canada N1G 2W1, with the support of a sponsor: Will Taylor. The developed test is used to solve the problems of diagnosis, decision support and examining the simulated subject area by studying its model. The results of the study can be used by anyone, due to the fact that Eidos the universal automated system, which is a tool of ask-analysis, is in full open free access on the author's website at: http://lc.kubagro.ru/aidos/_Aidos-X.htm, and numerical examples of solving veterinary problems with the use of artificial intelligence technologies are placed as a cloud Eidos-application 129
The article dwells on the features of the transition process in the transformer windings under non-zero conditions typical for the modes of drying the trans-former with low frequency currents. The article also shows the effect of non-zero conditions on the cur-rents in the windings and the heating of the windings
In the article, we discuss some of the causes of plant death during the introduction in the south of Primorsky Krai. Introduction studies involving a large number of plant species always have not only positive, but also negative results. According to the results of long-term observations, factors affecting the longevity of plants in the arboretum collection of Gornotayezhnaya station of the FEB RAS are indicated. Regular inventories and annual observations of introduced species in the arboretum of Gornotayezhnaya station of the FEB RAS allowed us to identify the reasons of the decline of introduced species from the arboretum collection: low winter hardiness, fragility, inhibition of plants in thickened planting and due to shading, mismatch of environmental factors, insufficient care, damage to animals. It has been established that for the purpose of the initial selection of new species for introduction research, it is possible to use climatic zoning according to Reder. Analyzing the species belonging to a certain temperature zone and the reason of the decline, it can be conclude that the species belonging to I-III zones in the conditions of the southern Primorye acclimatize quite successfully with good care and a properly selected landing site. Plant species belonging to the IV-V zones need protection from low temperatures at a young age, and some in adulthood. The temperature zones VI and VII are critical for successful acclimatization, so the species belonging to these zones require special attention and careful selection of the place of growth
The article considers the phenomenon of the formation of giant fruits in higher plants. As for the size of plants, several positions are distinguished such as giant fruit, a common habit, i.e. the size of the whole plant; or the harvest. Examples of different types of gigantism in plants are given. It is shown that since the ancient times the phenomenon of gigantism has been widespread. Various reasons for the appearance of abnormally large plants and fruits are considered, growth genes regulating the process of cell division and formation are given. The study of the connections between plant organs that conduct and consume the assimilant, redistribute and use carbon is easy in the concept of donor-acceptor relationships. As a model object for studying the growth of giant fruits, we have examined varieties of pumpkin called Cucurbita maxima. Thus, in a short period, a paradigm shift occurred. From surprise and enthusiasm for the gigantism of plants, the issue of cellular growth regulation, the criteria for evaluating the reproduction process, the search for links with the size, number of cells and their properties is become. New model objects have appeared, the analysis of images of plant giants has shown a unique visual resource on various objects. New knowledge about the growth of plants and the ability to regulate the process formed the basis for selection programs for obtaining vegetables with specified fruit parameters, which makes them competitive in the market today
The article describes experimental material obtained in two long-term experiments of Kubsau on the impact of certain agricultural practices on the basic agrophysical and water indicators of leached black humus, heavy duty. The study was conducted in two agricultural landscapes of lowland rainfed and irrigated lowland-basin. The state of the agrophysical properties of the arable land in two districts of the region was given by Institute "Kuban Niigiprozems". We have established that during prolonged agricultural use, the biggest change of the parameters of the agrophysical properties occurred in the arable layer 0-20 cm. Particularly noticeable compaction was observed on meadow-black soils of lowland-basin agricultural landscapes. The condition of soil structure and the density of the composition influence of level of soil fertility and the system of primary tillage. With the increase in the level of fertility, the coefficient of structure increases. The density of the composition of the studied cultures are much more dependent on tillage to the end of vegetation, especially high is 1,31-1,36 g/cm, it was given by direct sowing. The most destructive effect on soil structure was made by a surface treatment system. Organic fertilizers encourage soil compaction. Manure has a positive impact on the stock of productive moisture in two-meter layer on the background of deep processing
In the diagnosis of subclinical, serous and catarral mastitis of cows a wide range of microorganisms has been isolated, including the genera Streptococcus (Str. disgalactiae, Str. agalactiae, Str. pyogenes), Staphylococcus (St. aureus, St. epidermidis) и E. coli. When treating cows with mastitis with argovit, the average duration of treatment for animals was2.9 +0.1, 3.3 + 0.3, 4.1 +0,2 days, which is 1.6, 1.9, 1.8 times less compared with the drug spectromast in the control group. The effect of argovit containing silver nanoparticles on antibiotic sensitivity in selected microflora in the treatment of subclinical, serous and catarrhal mastitis of cows was studied. Studies conducted to determine the antibiotic sensitivity of isolated microorganisms after the treatment of mastitis of cows showed the dependence of the results, both on the drug used and on the clinical form of the disease. It has been established that the use of spectromast causes a pronounced decrease in19 (76%) preparations with subclinical, 24 (96%) serous and 23 (92%) catarrhal mastitis. It was also noted that with the development of the pathological process, an increase in the appearance of previously absent resistance to antibacterial drugs was observed with subclinical6 (24%), serous 7 (28%), catarrhal 8 (32%) mastitis of the cows. While using argovit, an increase in antibiotic sensitivity to 21 drugs (84%) in subclinical, 24(96%) in serous and 23 (92%) catarrhal mastitis was revealed. In the treatment of subclinical mastitis in the experimental group, a slight decrease was observed for 4 (16%) drugs