Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Tsatsenko Lyudmila Vladimirovna

Scholastic degree


Academic rank

professor

Honorary rank

Organization, job position

Kuban State Agrarian University
   

Web site url

Email

t-lemna@yandex.ru


Articles count: 62

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366 kb

SPIKE PRODUCTIVITY IMPLEMENTATION IN COLLECTION SAMPLES OF WINTER SOFT WHEAT

abstract 1572003021 issue 157 pp. 282 – 292 31.03.2020 ru 22
The work is devoted to studying the productivity of an ear of winter soft wheat samples from the collection of the All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov (VIR). The study attempted to conduct a pre-selection study of collection material on the elements of spike productivity. The concept of pre-selection research is based on the study of initial collection material according to given characteristics in order to identify the best genotypes for further use in breeding practice. In the analysis of the collection material, the following characteristics were used as indicators of spike productivity: length of the main spike (cm), number of spikelets in the main spike (pcs), number of grains in the main spike (pcs), grain weight from the main spike (g), 1000-kernel weight (g). Plant height was one of the basic indicators of the studied material, because a number of samples differed in alleles of short-stem genes. According to the results of two years of research, according to the indicator «1000-kernel weight», the varietal sample Cologna lunga razza 188 (Italy) was identified as 71,20 g. According to the indicator «number of grains in an ear», the varietal sample Fenotipo 1 (Italy) was 57,3 g. Also according to the totality of indicators, the varietal sample Kaloyan (Bulgaria) with an average of two years of research with a 1000-kernel weight – 51,77 g, the number of grains in an ear – 51,6 pcs. and a mass of grains per ear – 2,71 g.
231 kb

USING REAL-TIME PCR HYBRIDIZATION PROBES, ON THE EXAMPLE, OF A MARKER OF THE TMV RESISTANCE GENE IN A TOMATO CULTURE

abstract 1582004012 issue 158 pp. 168 – 180 30.04.2020 ru 26
The article discusses the method of selection using markers in the selection of parent pairs for hybridization, in the selection process and in the subsequent evaluation of parent lines, used in linear and backcross selection of tomatoes. The work was performed on tomato hybrids that combine resistance genes to tomato mosaic virus (ToMV) or tobacco mosaic virus (TMV) in their genome. The research used seeds of foreign F1 tomato hybrids recommended by the manufacturer as resistant to the tobacco mosaic virus. Most of the tomato hybrids were Dutch selection, such leading firms as De Ruiter Seeds, Enza Zaden, Rijk Zwaan. The aim of the work was to test a system of hybridization probes designed to identify a gene for resistance to the tobacco mosaic virus. The study of the genotype of tomato plants was carried out in the laboratory of molecular diagnostics of plants , equipped with modern devices for PCR analysis on the basis of NIIOZG (Krymsk). Plant material was collected in different phases of plant vegetation. pre-numbered 1.5 ml test tubes (SSI-1200-00), plastic tablets, tweezers, and distilled water were used to collect plant samples. The collected samples were stored in a refrigerator until the plant DNA was isolated. During the study, collectible tomato samples were studied, including 8 F1 hybrids and 2 lines used as controls. According to the results of genetic analysis using the marker of the TMV resistance gene, it was found that all hybrids and one control line # 175/14 have a resistance gene in their genotype, and the analysis also showed the state of the gene – homozygous or heterozygous. The results of artificial infection confirmed the results obtained by PCR analysis. At the same time, using genetic research, it is possible to determine the exact state of the gene in the plant, which cannot be determined by methods of artificial infection, since no visual differences were found between plants with a heterozygous and homozygous state of the gene. The created marker and PCR analysis method can be recommended for wide application in the selection process of tomato culture. As a result of research, it was found that modern tomato hybrids have a gene for resistance to tobacco mosaic virus (TMV) in their genotype. This was determined by genetic analysis and confirmed by a test using artificial infection
629 kb

GIANT FRUITS OF PLANTS AS MODEL OBJECTS IN BREEDING RESEARCHES

abstract 1411807014 issue 141 pp. 65 – 76 28.09.2018 ru 71
The article considers phenomenon of the formation of giant fruits in higher plants. As regards the size of plants, several positions are distinguished such as giant fruit, in a common habit, i.e. the size of the whole plant; depending on the harvest. Examples of different types of gigantism in plants are given. It is shown that since the ancient times the phenomenon of gigantism has been widespread. Various reasons for the appearance of abnormally large plants and fruits are considered, growth genes regulating the process of cell division and formation are given. The study of the connections between plant organs that conduct and consume the assimilant, redistribute and use carbon is easy in the concept of donor-acceptor relationships. As a model object for studying the growth of giant fruits, we have examined varieties of pumpkin called Cucurbita maxima. Thus, in a short period, a paradigm shift occurred. From surprise and enthusiasm for the gigantism of plants, the issue of cellular growth regulation, the criteria for evaluating the reproduction process, the search for links with the size, number of cells and their properties is become. New model objects have appeared, the analysis of images of plant giants has shown a unique visual resource on various objects. New knowledge about the growth of plants and the ability to regulate the process formed the basis for selection programs for obtaining vegetables with specified fruit parameters, which makes them competitive in the market nowadays
1294 kb

GIANT FRUIT – THE ICONOGRAPHY AND THE HISTORY OF THE ISSUE

abstract 1421808015 issue 142 pp. 26 – 40 31.10.2018 ru 106
The article considers the phenomenon of the formation of giant fruits in higher plants. As for the size of plants, several positions are distinguished such as giant fruit, a common habit, i.e. the size of the whole plant; or the harvest. Examples of different types of gigantism in plants are given. It is shown that since the ancient times the phenomenon of gigantism has been widespread. Various reasons for the appearance of abnormally large plants and fruits are considered, growth genes regulating the process of cell division and formation are given. The study of the connections between plant organs that conduct and consume the assimilant, redistribute and use carbon is easy in the concept of donor-acceptor relationships. As a model object for studying the growth of giant fruits, we have examined varieties of pumpkin called Cucurbita maxima. Thus, in a short period, a paradigm shift occurred. From surprise and enthusiasm for the gigantism of plants, the issue of cellular growth regulation, the criteria for evaluating the reproduction process, the search for links with the size, number of cells and their properties is become. New model objects have appeared, the analysis of images of plant giants has shown a unique visual resource on various objects. New knowledge about the growth of plants and the ability to regulate the process formed the basis for selection programs for obtaining vegetables with specified fruit parameters, which makes them competitive in the market today
159 kb

MULTIFLOROUS FORMS OF BREAD WINTER WHEAT AS A MODEL OBJECT IN THE RE-SEARCH OF THE REPRODUCTIVE POTENTIAL OF THE MAIN EAR

abstract 1401806024 issue 140 pp. 100 – 111 29.06.2018 ru 120
The article deals with the realization of the re-productive potential of wheat with the use of multi-flower formation as a model object of winter soft wheat plants. The essence of the concept of "reproductive potential" is disclosed, as well as the significance of this concept for research aimed at increasing the productivity of wheat plants. The data obtained during the study and analysis of varieties of winter soft wheat with the sign of multi-flower formation are given. The reproductive potential of winter soft wheat plants was determined by counting flowers at the VI stage of organogenesis. The examined multi-flower forms of soft winter wheat compared with Krasnodar breeding varieties showed increased reproductive potential, as well as an increased number of grains with the ear, but with a reduced mass of 1000 grains. According to the data obtained, the degree of the reproductive potential of multi-flower forms of winter soft wheat varied significantly, depending on the weather and climate conditions of the year of the study. In conclusion, the choice of multi-flower formation of winter soft wheat as a model object for investigating the reproductive potential of wheat, as well as the factors that influence its implementation and the extent of such influence is justified
182 kb

STUDY OF THE COLLECTION OF HYBRIDS OF TOMATO F1 WITH RESISTANCE TO POWDERY MILDEW FOR GROWING IN WINTER GLASS GREENHOUSES

abstract 1501906016 issue 150 pp. 143 – 155 28.06.2019 ru 120
Modern domestic tomato hybrid should be competitive with tomato hybrids of foreign selection. To create a model of a future hybrid, a collection of modern tomato hybrids of domestic and foreign selection presented on the seed market was investigated. During the study, a modern method of genetic study of the genotype of tomato Real-Time PCR was used. As a result of the study, a model of a modern tomato hybrid with tolerance to powdery mildew of tomato was described. In the future, it is planned to carry out breeding work to create such a hybrid. In the course of studying the F1 collection of tomato hybrids, it was found that the modern model of a tomato hybrid should have the following characteristics: a semi-vegetative type of growth with closely spaced internodes, early and medium ripening, a simple type of inflorescence with 4–5 fruits if the average weight of fruit is more than 200 grams; with 6–7 fruits weighing up to 150 grams for collection by tassels. High strength of fruits with the absence of concentric and radial cracking, suitable for long-distance transportation, is required. In the genotype of the modern hybrid model, in addition to tomato powdery mildew resistance (Oidiun lycopersici), it is necessary to have a complex of resistance genes to such diseases as tobacco mosaic virus (Tomato mosaic tobamovirus), Fusarium oxysporum, verticillosis wilt of the tomato; ), brown spot of tomato (Cladosporium fulvum). It is also desirable the presence of a gene of resistance to the yellow leaf curl virus of tomato (Tomato yellow leaf curl virus) and the gall nematode (Meloidogyne incognita)
469 kb

THE CARTOON «CHUDESNITSA» AS A RESOURCE OF INFORMATION OF THE HISTORY OF AGRICULTURE IN THE SOVIET PERIOD

abstract 1291705055 issue 129 pp. 746 – 757 31.05.2017 ru 179
In the article, the methods of representation, popularization and propagation of scientific achievements of agronomy in the Soviet period are considered. This topic may be interesting not only as an example of methods for educating young people about the current achievements of agriculture, but also serves as a valuable source of knowledge on the history of agronomy. This article considers the use of such of conveying information as a cartoon. Along with such forms of fine arts as: a poster, textiles, postage stamps and lacquer miniature, a cartoon is an example of data visualization. Having a background of an easy and sometimes jocular narrative, the necessary information is delivered to the viewer. After watching the cartoon, a person who is not previously acquainted with this issue will already have a general idea about it. A specialist, paying attention to details, is able to see and highlight the information necessary for himself, such as history and peculiarities of cultivation of the culture. In this article, as an example, we have considered the Soviet cartoon "Chudesnitsa." It tells about the appearance of corn in our country and the reasons for its sharply increased popularity. The article lists scenes of the cartoon that carry information directly on the history and technologies of agriculture, as well as on the accompanying industry. The effectiveness of this method of information transmission to people of all ages and professions is noted, as well as multitasking of its use in the educational program of specialists
287 kb

VISUAL PHENOTYPING IN PLANT BREEDING

abstract 1281704071 issue 128 pp. 1038 – 1050 28.04.2017 ru 199
The article is devoted to visual analysis and its application in the selection process. New possibilities of application of visual display of information in the research process are considered. We have presented the examples of the use of visual phenotyping in breeding, evaluated the advantages and disadvantages in this area. In particular, the algorithm of the program of LHDetect for estimating leaf pubescence of wheat wafers is described, based on the analysis of digital images of the sheet bend. We have also considered Phenotiki platform that uses digital images of the profile of plants for visual phenotyping to assess plant growth. Based on the analysis of various studies it was noted that the application of visual analysis for some purposes is very effective, but for others, on the contrary, not effective. We highlight the actual problems of proper representation and converting information. The article shows the various causes of loss and distortion of data, as well as possible solutions to these problems. The work describes the application of ASK-analysis in the selection process. As an example, it describes the evaluation of the polymorphism of variety groups of grapes. We have presented interesting solutions and ways to minimize distortion and loss of information in the process of its transformation found by the authors of these works. The article reveals the concept of "computational biology" and describes fundamental differences from other areas as well as tasks, problems, possible research areas and promising directions of development of computer biology
887 kb

THE TECHNIQUE OF VISUAL ANALYSIS IN THE HISTORY OF PLANTS ON THE EXAMPLE OF PAINTINGS BY I. MASHKOV

abstract 1321708014 issue 132 pp. 176 – 190 31.10.2017 ru 203
In the article on the example of painterly works of the artist I.I. Mashkov there have been represented the analysis of the plants’ history. The emphasis on the image as the object of study, in the modern world has become popular for several reasons. On the one hand, it is a documentary confirmation of events, the state of the object, and sometimes the only proof of a bygone era. On the other hand, the works of art are multifaceted information about the study area, recorded by the artist with a thorough drawing of details, environment, transfer of colors, shapes. The experience of creating electronic databases as a library of electronic resources is considered. The analysis of I. I. Mashkov's art paintings made it possible to identify several semantic blocks such as the species and genetic diversity of vegetable crops; spread of cultures, i.e. introduction on the example of pumpkins, tobacco and sunflower; pottery culture (indoor flowers, species and genetic diversity of camellia, begonias), local strawberry varieties with the phenomenon of fascia, and the production of bread with variety and characteristic forms. Through the analysis of still-life paintings and part of the portraits, in the artist's desire to "express time," a peculiar "species record" of agrocultures and agro-production was created in the period from 1910 to 1940 years
204 kb

STUDYING THE REPRODUCTIVE POTENTIAL OF PLANTS OF BEZOSTAYA 1 WHEAT HAVING ADDITIONAL SPIKELETS ON THE LEDGE OF THE RACHIS

abstract 1201606046 issue 120 pp. 664 – 674 30.06.2016 ru 253
The article discusses the effect of additional spikelets in the variety of Bezostaya 1 on the realization of productivity of the examined plants. We traced the frequency of occurrence of multirov spikes and studied the effect of this phenomenon on the productivity and the main quantitative characteristics of the studied plants. The article demonstrates the structure distinction of productivity for abnormal and normal ears. Experience was one-way vegetation, planted in triplicate, time of experiment - 2013 - 2015 years. The study revealed that the most frequent was the emergence of 1-4 additional spikelets per spike. Some quantitative characters had a slight deviation, these include the number of spiked ledges on the kernek, spike length and plant height. To a large extent we increased the characteristic values such as the number of grains per ear, number of spikelets per spike, number of grains onto the ledge, head weight and the weight of grains spike. We significantly reduced weight of 1000 grains. It was revealed that more localized spikes were mostly in the middle, the most productive part of the ear. As a result, changes have affected not only the quantitative characteristics related to the productivity of the ear, but also its architectonic whole
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