Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Коnоnеnко Sergey Ivanovich

Scholastic degree


Academic rank

professor

Honorary rank

—

Organization, job position

Kuban State Agrarian University
    North-Caucasus Research Institute of Animal
   

Web site url

—

Email

Kononenko@nm.ru


Articles count: 32

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PREVENTION OF VIOLATIONS OF METABOLISM IN FRESH COWS

abstract 1361802030 issue 136 pp. 189 – 200 28.02.2018 ru 466
The main diseases of lactating cows occur in the first two months of lactation, i.e. during the production peak of productivity. They are caused by the changes in metabolism during the transition period, which are not supported by adequate changes in the organization of feeding, or rather sufficient to ensure cows with nutrients, which causes a number of closely related diseases. To allocate any of them is not possible, as their manifestation depends on a number of reasons, and depending on situation may prevail one or the other. Among the most common diseases, there are ketosis, milk fever (paresis of maternity), dysplasia of the abomasum, acidosis, mastitis, endometritis, laminitis and leukemia. These diseases cause the greatest problems in herds with high productivity, and are caused by changes in metabolism in cows in the transition period and the inability to properly feed the animals at this time. To identify the causes of these diseases we do not need to study each of them individually - it is enough to deal with the peculiarities of metabolism. This period includes the month before calving and the first or second month of lactation, but the most important are 3 weeks before calving and 3 weeks later. In order to prevent metabolic disorders there were developed and tested feed for feeding cows before calving and immediately after calving. Their effect was studied on milk production and health status
1215 kb

CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY

abstract 1341710034 issue 134 pp. 404 – 426 29.12.2017 ru 1421
A method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES

abstract 1331709063 issue 133 pp. 823 – 850 30.11.2017 ru 804
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
211 kb

INCREASING OF BIOLOGICAL POTENTIAL IN POULTRY DUE TO THE USE OF PROBIOTICS

abstract 1271703036 issue 127 pp. 527 – 545 31.03.2017 ru 583
To improve the quality, safety and competitiveness of the domestic raw materials of animal origin the use of probiotics in the poultry industry is a priority and promising direction of the development of organic agriculture. One of the key problems of the production of probiotics is to provide actively stable preparations. Biocompatibility of the indigenous microflora with probiotics, medical form and individual range of antibacterial activity of probiotics have a significant impact on the introduction of probiotics in new habitat conditions and its effectiveness. Consideration of these factors can enhance the effectiveness of probiotics. Currently, the available literature has insufficient justification of certain doses of administration and methods of using different probiotic preparations. The materials present and summarize the results of the research concerning the use of probiotics in the poultry industry, based on the achievements of Russian and foreign scientists. In addition, based on the analysis of various sources of scientific literature, we suggest ways to solve the problem of probiotic therapy and give some recommendations on the organization of the technological process of selection and application of different probiotics in poultryfeeding. Raw meat from poultry reared with the use of probiotic preparations is safe, because metabolites of probiotic microorganisms are not accumulate in the body tissues. Researches of biosynthesis regularities of probiotics were made at the cellular level, including genetic aspects, features of regulation of secretion, dependence on the cultivation conditions, interaction with binding sites on the cell surface, participation in the vital activity of the population of individual cells. The use of probiotics contributes to normalization of the intestinal microflora, activation of protein metabolism, productivity increase, reduction of feed costs per unit of production and increasing of poultry industry economic efficiency as a whole
156 kb

THE APPLICATION OF THE BACTERIOPHAGES TO COMBAT COLIBACILLOSIS AND CAMPYLOBACTERIOSIS IN POULTRY

abstract 1231609029 issue 123 pp. 421 – 432 30.11.2016 ru 710
This work considers the needs and methods of phage therapy of two bacterial infections in poultry. The review examines the use of bacteriophages, from the moment of their discovery to the present day. We discuss the relevance of such diseases as compilobacteriosis and colibacteriosis transmitted from birds to humans, we show how to use bacteriophages and their efficacy in models of adult birds and chickens, including information provided on the pilot study of phage therapy at a poultry farm. The reported study was partially supported by RFBR, research projects No. 16-44-230855 and No. 13-04- 00991 and № 16-44-230855-р_а
296 kb

ISOLATION AND SELECTION OF THE NON TRANSDUCING E. COLI BACTERIOPHAGES FOR ANTI-COLIBACILLOSIS DRUGS

abstract 1221608040 issue 122 pp. 554 – 566 31.10.2016 ru 925
The isolation of E.coli phages from samples of natural and waste water obtained during expeditions in the different regions of Russian Federation was carried out. The obtained phages (286 isolates) were tested for their ability to lyse the pathogenic strains of E. coli – pathogenic agents of pig colibacteriosis in Krasnodar region. The study was conducted of their ability to phage transduction, the molecular-genetic characterization and biotechnological parameters of selected bacteriophages. For first experimental design of veterinary drugs was selected 5 coliphages having no ability of plasmids transduction. It has been shown that all the investigated phages are representatives of T4-type phages of family Myoviridae. The reported study was partially supported by RFBR, research projects No. 08-04-99111, 09-04-10132, 16-44- 230855
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CONCENTRATION OF NUTRIENTS AND MACRONUTRIENTS IN MUSCLE AND LIVER OF YOUNG GEESE FED COMPLETE FEED WITH DIFFERENT LEVELS OF CRUDE FAT

abstract 1201606062 issue 120 pp. 914 – 923 30.06.2016 ru 564
The aim of the research was to study the impact of starter complete feed (CF) with different levels of crude fat on the chemical composition of muscle tissue and liver of young geese. During days 5-28 the poultry was fed CF with 5.1-8.1 % crude fat, from 29- to 60- day age – with 5.4 %. By increasing the level of crude fat in starter CF there was a trend to an increase of the phosphorus content in muscle samples of geese hip and thigh - by 4.4-9.6 %, in the liver - by 27.2-53.5 % (P≤0,05). The chest muscles of the young geese fed high-calorie CF had 2.8-6.0 % higher fat content, than that of counterparts in the first group. At the same time, there was a tendency to a decrease in the concentration of fat in the muscles of the hip and thigh - by 1.9-5.0 % (P˃0,05). Increased level of crude fat in CF did not have a negative impact on the accumulation of heavy metals in the muscle and liver of geese, the concentration of the studied elements was within the permissible level established for food products
217 kb

WAYS OF REDUCTION OF ADVERSE FEEDING EFFECT ON ANIMALS

abstract 1191605021 issue 119 pp. 293 – 312 31.05.2016 ru 424
The successful development of livestock requires maintaining and further increasing of the genetic potential, the basis for the manifestation of which is the adequite balanced feeding. Currently, one of the most urgent problems of livestock breeding is to find ways for reduction of the negative impact of various feeding factors on the animals. In industrial conditions, it is difficult to exclude various feed stresses, which lead to a decrease in productivity, survival rate and ill health of animals and poultry. The work summarizes the research in this direction, taking into account the achievements of domestic and foreign authors. A serious problem of feed producing companies and livestock farms is the grain and compound feed affection with fungi and their metabolic products - mycotoxins. Based on the results of the research of several authors, the ways of solving this problem were identified and recommendations on the reduction of the negative effect on the body were provided. It is noted, that one of the best and effective ways to reduce the negative effect of mycotoxins on the body is a method of administration of adsorbents in animal feed. But it should be taken into consideration that in order to maximize the protection against mycotoxins and minimize their harmful effects on the body of animals it is necessary to use preparations, which include adsorbents of organic and inorganic nature, as well as to comply with the dosage of their introduction into the diets of animals and poultry.The progressive forms of preparations of a new generation, solving problems of the negative impact of feeding factors, include probiotics, which contain some bacteria, immobilized on the solid particles, so that they inhabit the intestine faster. Modern biological complexes have immune correcting properties, increase non-specific resistance of the organism, normalize microbiocenosis of gastrointestinal tract, and stimulate the functional activity of the digestive system. Therefore, it is necessary to use feed additives with detoxification properties as part of animal compound feed, according to recommendations on their application in appropriate dosages
149 kb

EFFECT OF BENTONITE ON MEAT PRODUCTIVITY AND QUALITY OF PORK GILTS

abstract 1181604044 issue 118 pp. 773 – 782 29.04.2016 ru 730
In our country, great influence is given to the production and consumption of agricultural animals' meat with high consumer qualities. In this regard, the livestock producers, particularly pigs, are to increase production and improve product quality. Works on using some natural mineral complexes - bentonite and zeolites as a source of macro - and trace elements are performed. The aim of the research is to study the effects of bentonite feeding with free access on the meat productivity and hygienic meat quality of young pigs for fattening. Best effect on the slaughter indexes of fattening gilts had bentonite feeding with free access that resulted in significant superiority of the animals in the experimental group over the control counterparts on pre-slaughter live weight, slaughter weight, chilled carcass weight, slaughter yield, carcass length, the "muscle" eye area and the backgammon weight. More favorable effect in the longest muscle on the dry matter and protein synthesis was provided by bentonite feeding with free access. Due to this, the gilts of the experimental group were in significant advance of their control counterparts in concentration of dry matter and protein in meat. Along with this, the longest muscle of young pigs on fattening in the experimental group had the highest protein value, as according to the protein-qualitative indicator the animals of this group were in significant advance of the control counterparts. Feeding bentonite clay with free access had a strong detoxifying effect that is against control counterparts of gilts in the experimental group showed the significant reduce of lead, zinc and cadmium concentrations in the meat
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PRODUCTIVITY, DIGESTIVE METABOLISM IN YOUNG PIGS WHEN USING BENTONITE FEEDING

abstract 1181604045 issue 118 pp. 783 – 793 29.04.2016 ru 724
For further increasing pork production and to increase its quality it is necessary to provide complete and balanced feeding of young pigs for fattening. The special place takes the issue of satisfying their physiological needs in macro and micronutrients. The most cost-effective step in these circumstances is using bentonite clays as natural mineral supplements. The aim of this work was to study the effect of the addition of bentonite of Zamankulskaya field on the energy growth, digestibility and the digestibility of nutrients of the diet of young pigs. According to the results of the survey, the best productive effect was when ensuring free access to the bentonite, so that the young pigs of the 3rd experimental group vs control group had significant superiority according to gross and average daily live weight gain and feed products. According to the results of scientific and economic experience, it was found that with free access to the bentonite best economic and useful indicators were observed in the animals of the 3rd experimental group against the control analogs, as they had significant superiority in terms of absolute live weight gain and feed consumption per unit of production. During the physiological exchange of experience the most beneficial effect on the hydrolysis of complex organic compounds had bentonite feeding with free access of pigs. The result was a significant superiority of the experimental animals over the control ones in coefficients of digestibility of dry matter, organic matter, crude protein, crude fat and NFE. To improve the digestibility and absorption of nutrients in the diet of young pigs it is advisable to feed them with bentonite clay as natural mineral supplements with free access
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