Name
Suprun Ivan Ivanovich
Scholastic degree
•
Academic rank
—
Honorary rank
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Organization, job position
All-Russian Research Institute of Rice
Web site url
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Articles count: 22
Genetic studies of apricot are the actual direction in the genetics of fruit crops. In this regard, the improvement of the collection of SSR markers for the genotyping of this culture is an objectively significant task. In a study for the 16 SSR-markers previously developed on almonds (PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1, PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1) and Siberian apricot (A3-72, A1-63, H2-22, A3- 7-1, H2-5, A1-7, A3-9, H2-45), approbation and evaluation of the prospects of using for genotyping Prunus armeniaca L. were performed. Approbation, performed on 3 varieties of different origin, revealed markers and their combinations optimal for their use. During the study, all tested DNA markers were grouped into multiplex sets, including 4 markers. This allows carrying out genotyping simultaneously on 4 loci in the formulation of one reaction. One marker (PdUnchar2) from the studied sample included in the multiplex set did not show amplification. Five markers gave a monomorphic product. The remaining 11 SSR markers allowed us to obtain polymorphic, cultivar-specific SSR fingerprints for all the studied cultivar. These multiplex sets are proposed for use in studying the genetic polymorphism of the species Prunus armeniaca L.
This article presents the results of testing IRAP DNA
markers Cass1 and Cass2 applied to Prunus spinosa.
The findings suggest the high perspectiveness of
their using for the study of genetic diversity of the
gene pool of this species. According to the results of
the analysis of the sample 12 genotypes were
identified from 6 to 13 fragments in the spectrum of
Cass1 and from 5 to 11 fragments for Cass2. As a
result of cluster analysis in the sample formed three
groups of samples. In one of the groups, which is
most distant from the other two, includes samples
taken in Ukraine, while the remaining two groups
included samples from Armenia, the Krasnodar
region region, the Republic of Adygea, Ukraine and
Moldova, and three cultural large-fruited form. The
distribution of samples in clusters corresponded to
their geographical origin that favors the objective
assessment of genetic distances between the samples
using Cass1 and Cass2 markers. Thus, it was
concluded that the prospects of using DNA markers
to study the genetic diversity within a species of
Prunus spinosa
Industrial horticulture assumes the most effective use of
the potential of varieties. One of the key factors
determining the yield of garden plantings is the
effectiveness of pollination. To obtain the maximum
yield, it is necessary to ensure maximum pollination
during the flowering period. For this reason, much
attention has been paid to the selection of pollinators.
Crab-apple forms are promising for use as pollinators, so
this work was aimed at identifying the most common
alleles of the self-incompatibility gene in the crab-forms
using the molecular genetic method of analysis. The
object of the study was 29 apple-tree creams and 3 elite
selection forms. They carried out the molecular genetic
identification of alleles S2 and S10, which are among
the most common apple trees in the world gene pool.
Allele S2 was identified in 16 samples (14 forms and 2
elite selection forms), while S10 allele in one
sample (elite form 12/2-20 (24-28)). Data on the allelic
composition of the S gene in the samples studied are of
value for the formation of a genetic passport on the
compatibility of the studied samples of apple with
modern industrial varieties
Professional apple gardening is bound to particular
risks, of which is essential losses of a harvest because
of diseases. An apple scab, the caused Venturia
inaequalis (Cooke) G. Winter, brings the greatest
loss. The main approach in monitoring of a scab of an
apple-tree is creation of grades, steady against
pathogen. In the present work we have carried out
phytopathology testing of generations of the seedlings
received from the free pollination of six forms of a
crab of Malus orientalis from a collection MOS VIR
(Maykop) characterized by a relative resistance to the
apple scab in 2-3 classes of damage by long-term data. For infection we used inoculum, consisting both
of natural population of a scab, and of strains of
pathogen of various cultivars and geographical origin.
When carrying out padding infection increase in force
of an infectious background that can be bound to
selection of the plants of biotypes of a fungus that are
most adapted for genotypes was noted. It is
recommended for precise elimination of unstable
plants at selection at early stages of an ontogenesis to
carry out more than one serial infection during the
season. By results of the phytopathological testing,
we highlighted three Malus orientalis forms from the
six studied No. 17982, 17985 and 3080 the most
perspective stability genes for an introgressiya in a
cultural gene pool of an apple tree
Isolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
In the presented study, we have performed genotyping
of modern Russian rice cultivars using microsatellite
DNA-markers. The markers showed different level of
allelic polymorphism: from 2 to 8 alleles per locus. For
all studied cultivars,unique DNA-fingerprints were
obtained
The study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
In the course of the work, 33 ISSR markers were
evaluated for efficacy in the detection of genetic changes
in regenerants of Galanthus woronowii Losinsk.. Ten
markers were found suitable for genotyping according to
the species under study. Five samples from the selected
ten were analyzed for a sample of 20 plants of
regenerants and a mother plant. The obtained data testify
to genetic stability of plant material in the process of
microclonal propagation
Microsatellite DNA markers are currently used
effectively in the study of the genetic diversity of the
gene pool of fruit crops and DNA certification of
varieties. For plum now there is rather limited list of
works on the development of this type of DNA
markers. Most often, the search for new SSR-markers
for this species is carried out by checking of crossreproducibility
of SSR-markers developed in other
species of the genus Prunus. In this study, for the 18
SSR-markers previously developed on a peach, there
was performed testing and evaluation of the prospects
for the use of the genotyping of plum cultivars. Testing
was made on the 4 varieties of genetically distant,
belonging to the 4 different subspecies of Prunus
domestica L., showed the effectiveness of their use.
During the study, all tested DNA-markers were
grouped together in multiplex sets comprising 3-4
markers. This allows simultaneous genotyping of 3-4
loci in a single PCR reaction. These multiplex kits are
available for use in the study of genetic polymorphism
of species Prunus domestica L
Results of testing of multiplex sets SSR-markers for
genotyping of rice varieties are presented in the article.
Two sets of SSR-markers were formed:
1: RM1+ RM11+ RM70+RM122;
2: RM164+RM167+RM168.
The optimal combination of DNA markers in the
multiplex sets and PCR conditions allowed obtaining
accurate, easily interpretable results when performing
fragment analysis on automated genetic analyzer
ABIprism3130. Using multiplex sets, genotyping was
performed for several varieties of rice: domestic
breeding and one variety – IR36 from the breeding of
IRRI (Manila, Philippines). For all the studied
varieties specific SSR-fingerprints were obtained.
RM 168 marker showed in domestic varieties a low
level of polymorphism - one allele of 97 bp. However,
at the same time, the variety IR-36, showed a second
type of allele 107 bp. In addition, the loci of RM1,
RM11, RM167 and RM164 have unique alleles in this
variety. It is consistent with significant genetic
differences of these varieties and the rest of the
varieties in studied sample. The proposed SSR
multiplexes are promising for use in DNA certification
of rice varieties and assessment of genetic diversity