Scientific Journal of KubSAU

The wide variety of microorganisms has been identified in many wine-making countries on the berries of grapes. These are yeasts of different families, forms and kinds, bacterium, mold fungi. In the article, we present the results of investigating species composition of microflora of berries of white and red types of grape, which grows in different economies of the Krasnodar region and the republic of Abkhaziya. The sowings onto the elective media were conducted for the development of entire spectrum of yeast. The grown colonies after preliminary microscoping were separated into the cultures and subjected to testing according to the culturalmorphological signs, being guided by determinants and benefits. It was established the specific variety of microflora on the surface of the berries of grapes of all investigated types, without dependence on the place of their growth. Obtained data showed that the group of yeast, which constantly is present in the complex of the epiphytic microorganisms of grapes of Saccharomyces, Pichia, Hansenula, Hanseniaspora was characteristic for all types of grapes in all investigated regions. The heterogeneity of the taxonometric composition of microflora is shown. Prevailed yeasts were of family Saccharomycetaceae, form Saccharomyces vini. A quantity of yeast of Saccharomyces vini decreases in a number of Myskhako-Caucasus-Fanagoriya, that as a whole will be coordinated with the climatic conditions. Only the type of Pinot nuar grapes had yeasts of Brettanomyces Dekkera. On the berries of Cabernets and Karaburnu we have discovered yeasts of Schisosaccharomyces acidodevoratus, causing acid-reduction. On the berries of the grapes, which grew in joint stock company APF “Fanagoriya” we haven’t revealed the presence of lactic acid bacteria Lactobacillus brevis and yeasts of the form of Schisosaccharomyces acidodevoratus. In the same farm the smallest quantity of yeastswreckers is noted, which we the forms of Pichia and Candida. In the microflora of Cabernets, Risling and, especially, Karaburnu grapes, we have found lactic acid bacteria Lactobacillus plantarum and Lactobacillus brevis. Lactobacillus plantarum. Among the bacteria the total quantity of coccic flora composes - 56 %, the rod-shaped - 44 %. Two types of active acetous bacteria are identified – Acetobacter aceti and Acetobacter xelinum. Especially high was their surface concentration on the berries of Kachich type of grapes

In the presented study, we have performed genotyping of modern Russian rice cultivars using microsatellite DNA-markers. The markers showed different level of allelic polymorphism: from 2 to 8 alleles per locus. For all studied cultivars,unique DNA-fingerprints were obtained