Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Kovalev Victor Savelyevich

Scholastic degree


Academic rank

Honorary rank

Organization, job position

All-Russian Research Institute of Rice
   

Web site url

Email

arrri_kub@mail.ru


Articles count: 6

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182 kb

APPROBATION OF MULTIPLEX SSRANALYSIS FOR DNA-FINGERPRINTS OF RICE VARIETIES

abstract 1141510103 issue 114 pp. 1420 – 1430 30.12.2015 ru 571
Results of testing of multiplex sets SSR-markers for genotyping of rice varieties are presented in the article. Two sets of SSR-markers were formed: 1: RM1+ RM11+ RM70+RM122; 2: RM164+RM167+RM168. The optimal combination of DNA markers in the multiplex sets and PCR conditions allowed obtaining accurate, easily interpretable results when performing fragment analysis on automated genetic analyzer ABIprism3130. Using multiplex sets, genotyping was performed for several varieties of rice: domestic breeding and one variety – IR36 from the breeding of IRRI (Manila, Philippines). For all the studied varieties specific SSR-fingerprints were obtained. RM 168 marker showed in domestic varieties a low level of polymorphism - one allele of 97 bp. However, at the same time, the variety IR-36, showed a second type of allele 107 bp. In addition, the loci of RM1, RM11, RM167 and RM164 have unique alleles in this variety. It is consistent with significant genetic differences of these varieties and the rest of the varieties in studied sample. The proposed SSR multiplexes are promising for use in DNA certification of rice varieties and assessment of genetic diversity
332 kb

CREATION OF RICE BREEDING LINES, CARRYING BROAD SPECTRUM BLAST RESISTANCE GENE Pi-40 USING DNA-MARKERS METHODS

abstract 0861302018 issue 86 pp. 268 – 278 28.02.2013 ru 1070
The results of development of rice breeding lines, carrying the wide range resistance gene to rice blast disease - Pi-40. For identification of the dominant allele of the gene the DNA - marker analysis was used. With co-dominant DNA markers plants from inbred populations that carry a dominant allele of this gene in the homozygous state were selected
727 kb

DEVELOPMENT OF MULTIPLEX DNA-MARKER SET FOR IDENTIFICATION OF RICE BLAST RESISTANCE GENES Pi -40 AND Pi-b

abstract 0941310019 issue 94 pp. 252 – 263 27.12.2013 ru 837
Multiplex DNA-marker set for PCR identification for rice blast resistance genes Pi-40 and Pi-b was developed in this study. Optimal primers combinations and PCR conditions allows to identify both abovementioned genes in the single PCR
235 kb

EFFECT OF "REASIL UNIVERSAL" MULTICOMPONENT FERTILIZER ON RICE YIELD

abstract 1021408021 issue 102 pp. 338 – 350 31.10.2014 ru 911
The efficacy of using "Reasil Universal" multicomponent fertilizers on the rice crops. It is found that the greatest effect for seed treatment before sowing and foliar feeding of plants was reached at a dose of 300 ml/t or ml/ha. This gives better grain yield of rice, respectively, 5, 43 and 5, 79 kg / ha
2040 kb

ESTIMATION OF RICE HYBRID LINES WITH BLAST RESISTANCE GENES PI-B, PI-Z AND PI-40 FOR RESISTANCE TO RICE BLAST (PIRYCULARIA ORYZA) ISOLATES, COLLECTED IN THE KRASNODAR TERRITORY

abstract 0761202057 issue 76 pp. 692 – 701 29.02.2012 ru 1380
Estimation of blast resistance of rice breeding lines, carrying resistance genes Pi-b, Pi-z and Pi-40 has been done. For phytopatological test isolates of pathogen from Krasnodar territory were used. As the results of the study Pi-40 gene showed maximal resistance to blast isolates. On the other part, rice line, carrying both genes – Pib and Pi-z showed significantly higher level of resistance with compare to standart variety Khazar
295 kb

ДНК-ПАСПОРТИЗАЦИЯ СОВРЕМЕННЫХ РОССИЙСКИХ СОРТОВ РИСА С ПРИМЕНЕНИЕМ SSR-МАРКЕРОВ

abstract 1311707065 issue 131 pp. 772 – 782 29.09.2017 ru 229
In the presented study, we have performed genotyping of modern Russian rice cultivars using microsatellite DNA-markers. The markers showed different level of allelic polymorphism: from 2 to 8 alleles per locus. For all studied cultivars,unique DNA-fingerprints were obtained
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