Name
Kovalev Victor Savelyevich
Scholastic degree
•
Academic rank
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Honorary rank
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Organization, job position
All-Russian Research Institute of Rice
Web site url
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Articles count: 6
In the presented study, we have performed genotyping
of modern Russian rice cultivars using microsatellite
DNA-markers. The markers showed different level of
allelic polymorphism: from 2 to 8 alleles per locus. For
all studied cultivars,unique DNA-fingerprints were
obtained
Estimation of blast resistance of rice breeding lines, carrying resistance genes Pi-b, Pi-z and Pi-40 has been done. For phytopatological test isolates of pathogen from Krasnodar territory were used. As the results of the study Pi-40 gene showed maximal resistance to blast isolates. On the other part, rice line, carrying both genes – Pib and Pi-z showed significantly higher level of resistance with compare to standart variety Khazar
The efficacy of using "Reasil Universal" multicomponent fertilizers on the rice crops. It is found that the greatest effect for seed treatment before sowing and foliar feeding of plants was reached at a dose of 300 ml/t or ml/ha. This gives better grain yield of rice, respectively, 5, 43 and 5, 79 kg / ha
Multiplex DNA-marker set for PCR identification for rice blast resistance genes Pi-40 and Pi-b was developed in this study. Optimal primers combinations and PCR conditions allows to identify both abovementioned genes in the single PCR
The results of development of rice breeding lines, carrying the wide range resistance gene to rice blast disease - Pi-40. For identification of the dominant allele of the gene the DNA - marker analysis was used. With co-dominant DNA markers plants from inbred populations that carry a dominant allele of this gene in the homozygous state were selected
Results of testing of multiplex sets SSR-markers for
genotyping of rice varieties are presented in the article.
Two sets of SSR-markers were formed:
1: RM1+ RM11+ RM70+RM122;
2: RM164+RM167+RM168.
The optimal combination of DNA markers in the
multiplex sets and PCR conditions allowed obtaining
accurate, easily interpretable results when performing
fragment analysis on automated genetic analyzer
ABIprism3130. Using multiplex sets, genotyping was
performed for several varieties of rice: domestic
breeding and one variety – IR36 from the breeding of
IRRI (Manila, Philippines). For all the studied
varieties specific SSR-fingerprints were obtained.
RM 168 marker showed in domestic varieties a low
level of polymorphism - one allele of 97 bp. However,
at the same time, the variety IR-36, showed a second
type of allele 107 bp. In addition, the loci of RM1,
RM11, RM167 and RM164 have unique alleles in this
variety. It is consistent with significant genetic
differences of these varieties and the rest of the
varieties in studied sample. The proposed SSR
multiplexes are promising for use in DNA certification
of rice varieties and assessment of genetic diversity