Name
Tokmakov Sergey Vyacheslavovuch
Scholastic degree
•
Academic rank
—
Honorary rank
—
Organization, job position
State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
Web site url
—
—
Articles count: 10
Molecular markers for allele identification of breeding important rice genes Pi-ta (blast resistance) and Rc (red pericarp) have been created. Their efficiency is also checked
Estimation of self-incompatibility allelic diversity in Russian apple varieties was conducted. We have established S-allele gene set for a number of varieties. The most common allele of gene was identified. Alleles frequency data obtained are consistent with those in the world apple germplasm
As the result of the work, the test of microsatellite DNA markers for the identification of genomic polymorphism between apple cultivars Florina, Golden Delicious and clonal forms has been done. With the high level of intervarietal polymorphism, studied DNA markers showed no allelic differences between varieties and their clonal forms
In the process of the research we had accomplished an analysis of allelic polymorphism of self-incompatibility in pears in demand in the production and breeding of modern varieties of pears of North-Caucasian Zonal Research Institute of Horticulture and Viticulture collection of genetic resources. In the first stage the consensus primers PycomC1F and PycomC5R were used. With obtained data, after identification of the alleles studied in varieties using consensus primers an allele specific S5 and S8 DNA markers were used for confirming the presence / absence of data allele studied cultivars
This study was aimed on testing of IRAP markers developed
at plum on varieties of peach, Russian plum and cherry plum and genotyping of home and foreign varieties of plum, followed by an analysis of the data. During the testing of the markers have been identified as a high level of polymorphism between genotypes of plum and between the studied species. On the basis of the results obtained by genotyping 15 samples of Prunus was built dendrogram. Cluster analysis was divided into 3 groups corresponding varieties of P. persica, P. domestica and a common group for varieties of Russian plum and cherry plum
The study was performed to genotype some
commercial wine yeast strains using the assay of Interdelta
genomic sequences. Experimental parameters of
PCR to identify were optimized and optimal simplified
method of DNA extraction from dried preparations of
yeast cultures was define. Proven method showed a
high level of resolution and can be used for the
analysis of genetic diversity wine yeast in combination
with SSR-markers
Microsatellite DNA markers are currently used
effectively in the study of the genetic diversity of the
gene pool of fruit crops and DNA certification of
varieties. For plum now there is rather limited list of
works on the development of this type of DNA
markers. Most often, the search for new SSR-markers
for this species is carried out by checking of crossreproducibility
of SSR-markers developed in other
species of the genus Prunus. In this study, for the 18
SSR-markers previously developed on a peach, there
was performed testing and evaluation of the prospects
for the use of the genotyping of plum cultivars. Testing
was made on the 4 varieties of genetically distant,
belonging to the 4 different subspecies of Prunus
domestica L., showed the effectiveness of their use.
During the study, all tested DNA-markers were
grouped together in multiplex sets comprising 3-4
markers. This allows simultaneous genotyping of 3-4
loci in a single PCR reaction. These multiplex kits are
available for use in the study of genetic polymorphism
of species Prunus domestica L
The study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
In the presented study, we have performed genotyping
of modern Russian rice cultivars using microsatellite
DNA-markers. The markers showed different level of
allelic polymorphism: from 2 to 8 alleles per locus. For
all studied cultivars,unique DNA-fingerprints were
obtained
Industrial horticulture assumes the most effective use of
the potential of varieties. One of the key factors
determining the yield of garden plantings is the
effectiveness of pollination. To obtain the maximum
yield, it is necessary to ensure maximum pollination
during the flowering period. For this reason, much
attention has been paid to the selection of pollinators.
Crab-apple forms are promising for use as pollinators, so
this work was aimed at identifying the most common
alleles of the self-incompatibility gene in the crab-forms
using the molecular genetic method of analysis. The
object of the study was 29 apple-tree creams and 3 elite
selection forms. They carried out the molecular genetic
identification of alleles S2 and S10, which are among
the most common apple trees in the world gene pool.
Allele S2 was identified in 16 samples (14 forms and 2
elite selection forms), while S10 allele in one
sample (elite form 12/2-20 (24-28)). Data on the allelic
composition of the S gene in the samples studied are of
value for the formation of a genetic passport on the
compatibility of the studied samples of apple with
modern industrial varieties