Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Milovanov Aleksander Valerievich

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Krasnoyarsk State Agricultural University
   

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Articles count: 10

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637 kb

EXTRACTING DNA BY NUCLEOSPINPLANT 2 COREKIT. COMMENTS

abstract 1011407160 issue 101 pp. 2376 – 2390 30.09.2014 ru 435
This review article highlights a new method of DNA extraction from herbarium leaves of grapes for use in PCR-analysis
563 kb

EXTRACTING DNA WITH USING PEQGOLD PLANT DNA MINI KIT

abstract 0901306011 issue 90 pp. 167 – 176 30.06.2013 ru 831
In the present article, the new method of DNA extraction from fresh and herbarium leafs of grape to their subsequent sequencing was described
516 kb

GENOTYPING OF GRAPE VARIETIES WITH USING MICROSATELLITE SSr MARKERS

abstract 0961402005 issue 96 pp. 53 – 65 28.02.2014 ru 683
This article presents the results of genotyping of new perspective table and technical varieties and grapes protoclones. It was established that varieties of Citrine, Helios, Arcadia pink and Preobragenie show differ genetic diversity in four loci
4445 kb

GENOTYPING OF NEW PERSPECTIVE WINE GRAPES WITH USING MICROSATELLITE MARKERS

abstract 0981404010 issue 98 pp. 137 – 155 30.04.2014 ru 1103
In this article we have described our work on genotyping of new ten productive wine grape protoclones
695 kb

GENOTYPING OF THREE NEW PRODUCTIVE WINE VARIETIES WITH USING MICROSATELLITE MARKERS

abstract 0981404077 issue 98 pp. 1073 – 1087 30.04.2014 ru 1140
In this article we have described a study of genotyping of new three productive wine grapes protoclones: Rhine Rieslin, Verdot black and Johanniter
1208 kb

PROGRESS IN GERMPLASM IDENTIFICATION AND GENOTYPING METHODS IN THE STUDY OF THREE TABLE GRAPE VARIETIES

abstract 0901306049 issue 90 pp. 735 – 753 30.06.2013 ru 625
In the present article, we have described data of comparative ampelography of biometric evaluation of leaf parameters of the three table grapes: Preobragenie, Victor and Jubiley Novocherkassk, widespread in the amateur and farming areas of Russia and the Ukraine. Showed results of molecular genetic analysis of DNA from these table grapes
493 kb

STRUCTURAL AND PHYLOGENETIC ANALYSIS OF VVMYBA1, VVMYBA2 AND VVMYBA3 GRAPEVINE GENES

abstract 1341710026 issue 134 pp. 303 – 327 29.12.2017 ru 75
The article presents the structural and phylogenetic analysis of VIT_02s0033g00410, VIT_02s0033g00390 and VIT_02s0033g00450 genes of the grapevine genome and the closely related orthologous gene MYB114 of the Arabidopsis genome. These genes are responsible for the biosynthesis of anthocyanin in the organs of model plants and are of interest not only for practical production and breeding, but also for fundamental research. These genes were analyzed for GCcomposition of nucleotides, the presence of cisregulatory elements and promoter regions. DNA and protein sequences were aligned to look for similar elements, which allowed further analysis of the ultraconservative domains of four genes. Based on the results of search and identification of the conservative regions, a cluster tree was constructed, which made it possible to identify the separation of gene sidelines from, presumably, the main one. At the same time, the construction of consensus trees based on DNA and protein sequences revealed their absolute similarity. "The Minimal Evolution Tree" allowed calculating the approximate dates of the appearance of the mutations and the divergence times of the gene branches between each other. At the same time, the appearance of the Vitis genus and its separation from the Rosales was taken as the time first divergence point. In the end, homologous metabolic pathways were searched between grapevine and Arabidopsis, which revealed the presence of homologous proteins in the grape proteome. In this turn, it already confirms the existence of similar biosynthetic pathways and, as a consequence, interactions such as "DNA-protein" and "protein-protein"
182 kb

STUDY OF INDIGENEOUS RUSSIAN GRAPE VARIETIES USING MICROSATELLITE MARKERS

abstract 0881304013 issue 88 pp. 196 – 205 30.04.2013 ru 803
The analysis of genetic polymorphisms of 12 autochthonous grape varieties grown in the National ampelographic collection of Russia (Anapa district of the Krasnodar region) through the study of allelic diversity at six microsatellite loci: VRZAG79, VVMD5, VVMD7, VVMD27, VRZAG62, VVS2 has been done. We have found that all native varieties have a unique set of allele. The assessment of genetic relationships varieties has been performed using cluster analysis. Data for DNA certification of the investigated genotypes of the grapes has also been obtained in the article
2838 kb

THE STUDY OF GRAPE GENETIC DIVERSITY OF THE NORTH CAUCASUS GENE POOL

abstract 1191605090 issue 119 pp. 1337 – 1355 31.05.2016 ru 125
The article presents the results of studies of 92 indigenous varieties and 15 wild genotypes by 24 microsatellite markers. As a result a locus database about germplasm in the collection stored at the Russian ampelographical collection AZOSViV (Anapa Zone Experimental Station of Viticulture and winemaking) was created. During the research of varietal breeding populations confirmed the validity in the production of the cultivar of Krasnostop anapsky with obvious changes in microsatellite sequences as a clone of famous indigenous Don cultivar Krasnostop Zolotovskii. This clone was selected in 1953 by prominent agrotechnologist Zotkin Ivan Ivanovich which is propagated by the author and his followers, and after a formal registration submitted to state testing in 2007 in GSK RF, zoned in the North Caucasus region (number 6) in 2014 year. Sort -clone Krasnostop anapsky only in the Kuban today cultivating in 35 hectares. By using molecular genetic techniques, now clonal selection of vine grapes is reasonably improving. Recent data collected by us in the work process, were analyzed a comprehensive manner, making it possible to draw conclusions about distinctness of grape varieties. Some clones were studied by DNA primers, based on retrotransposon sequences
5974 kb

THREE SIBLINGS OF MODERN PRIVATE VITICULTURE OF RUSSIA AND UKRAINE

abstract 0891305032 issue 89 pp. 465 – 484 29.05.2013 ru 1118
In the present article, we have described data of comparative ampelography of biometric evaluation of leaf parameters of the three table grapes: Preobragenie, Victor and Jubiley Novocherkasska, widespread in the amateur and farming areas of Russia and the Ukraine
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