Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Koshchayev Andrey Georgiyevich

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Academic rank

professor

Honorary rank

Organization, job position

Kuban State Agrarian University
   

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Articles count: 36

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590 kb

ALLELE-SPECIFIC PCR FOR TYPING OMPH-TYPES OF PASTEURELLA MULTOCIDA AND SEARCING FOR NEW APPROACHES TO STUDYING OF PATHOGENICITY OF BACTERIAL ISOLATES

abstract 1471903033 issue 147 pp. 180 – 197 29.03.2019 ru 55
Pasteurella multocida is an important respiratory pathogen of cattle. OmpH Protein is a major protective antigen of bacteria has been well studied in avian strains. In the literature there are no data available for the study of a variety of sequence of this protein among isolates with cattle respiratory pathology. There have been described several genes associated with the virulence of the bacterium in respiratory disease of cattle, but none of the authors compared the frequency of detection of these genes with the pathogenicity for laboratory animals. The aim of our study was the development of allele-specific PCR to determine Omph-types of Pasteurella multocida and the search for new approaches to assess the pathogenicity of isolates of bacteria. Total amount of 83 isolates allocated from the lungs of calves with respiratory pathology was investigated. All isolates belonged to groups A or D (isolates 63 and 20, respectively). Among isolates of capsular serogroup A we revealed 6 types, most propagation types were A1 and A2. All isolates of capsular serogroup D were one omph- type. In 16 out of 23 farms there were identified isolates of only one omph-type, 4 - 2 types, 3 - three types. The frequency of gene hgbb - hemoglobin binding protein correlated with pathogenicity of isolates for white mice. The developed allele-specific PCR along with hgbb gene detection can be used for screening and studying the properties of antigen and circulating pathogenic isolates and selecting a candidate vaccine strains
134 kb

ANIMAL HEALTH - CRITICAL FACTORS OF THE LIVESTOCK

abstract 0991405099 issue 99 pp. 1431 – 1442 30.05.2014 ru 1042
The article provides an overview of materials of conferences on problems and prospects of the industry of cattle breeding
161 kb

BIOLOGICAL FUNCTIONS OF CAROTENOIDS AT CATTLE REPRODUCTION

abstract 1291705080 issue 129 pp. 1113 – 1125 31.05.2017 ru 477
Carotenoid preparations are quite widely used in a health care, but in the veterinary and animal husbandry, their use is limited. In terms of livestock farms of the Krasnodar region and the Rostov region at the biochemical monitoring studies of the cattle blood the lack of carotene in the serum is revealed from 45 to 100 % of the samples. To study the biological function of carotenoid we used preparations, which are the oily solutions for injections: β-carotene (manufactured by FS 42-3867, which is produced from the biomass culture of the fungus Blakeslea trispora) and lycopene, emitted from plants. The cows in the test groups during the dry period and within two weeks after birth got carotenoid preparation subcutaneously at a dose of 10 ml per animal with an interval of 7 days. As a result, it was found out that the application of carotenoids has the most favorable conditions for the flow of labor and the postnatal period, which are manifested in the decrease in obstetric and gynecological diseases in cows and reduce the period of infertility. In the experimental groups the levels of vitamin A and carotene1,3 times higher (1,26 ± 0,14 μmol/L vs. 0,97 ± 0,09 μmol/L ) 1,8 times (0,34 ± 0 03 mg /% vs. 0.61 ± 0.11 mg /%) respectively in comparison with the parameters of the intact animals at a high level of confidence (P < 0,05). Phagocytic number and percentage of digestion of neutrophils increased by 8,5 %, the difference in the number of B-cells reached 9,1%, T-cells – 6,7%. In a humoral immunity found a significant increase in Ig A. After comparison of the level of lysozyme activity of blood serum was observed its reduction in all animals immediately after birth, but in the experimental groups this process was less pronounced. The use of carotene preparations helps to reduce the concentration of lipid peroxidation products in the body of cows (diene conjugates - by 16,6 %, ketodienes - by 35,7 %, malondialdehyde – by 11,3 %), which creates favorable conditions for the flow of the metabolic processes associated with ensuring the normal childbirth and post-partum involution of the genitals. In the group of cows, receiving the carotenoid lycopene, the dyspepsia morbidity of the newborn calves compared to a control decreased by 26,7 %, bronchopneumonia - by 13,3 %
250 kb

BIOTECHNOLOGY OF ORGANIC WASTE VERMICULTIVATION

abstract 0951401067 issue 95 pp. 1143 – 1172 30.01.2014 ru 999
This review contains the information about the role of annelids in maintaining soil fertility. Recommendations based on authors’ experience and scientific literature are given for vermicultivation, biomass use of Californian worms in animal husbandry and vermicompost use in crop production. Furthermore, detailed description of the methods of cultivation of worms is given
183 kb

BIOTECHNOLOGY OF TRICHODERMA-BASED FEED ADDITIVE WITH CELLULOLYTIC PROPERTIES

abstract 0931309078 issue 93 pp. 1146 – 1164 30.11.2013 ru 940
In the work, we have presented the information of elaboration of a manufacturing process of Mycocel feed additive with the cellulolytic activity for poul-try industry. Manufacturing process includes follow-ing steps: stock culture maintenance and storage of Trichoderma lignorum 81-17, growing fluid culture of microscopic fungus in sucrose yeast extract me-dium, feed additive with cellulolytic properties out-put and quality control, packaging, storage and disposal of waste. We have shown that the Mycocel is non-toxic feed additive for protozoa and warm-blooded animals (laboratory mice and quails). This study demonstrated total population livability in the experimental group with feed additive. Quail body-weight of experimental group was higher by 6% as compared to the control and feed consumption per 1 kg of live weight of bird was 3,58 kg, 7,5% lower than the control
433 kb

CHARACTERISTICS OF THE STATE OF SHEEP BREEDING IN RUSSIA AND THE ROSTOV REGION AND PROSPECTS FOR THE DEVELOPMENT OF THE INDUSTRY

abstract 1572003029 issue 157 pp. 392 – 410 31.03.2020 ru 6
The article provides a convincing argument for the preference of wool and fur products over artificial and synthetic textile fibers. It is noted that artificial fibers and synthetics have negative ionization, while natural wool has positive ionization. It is emphasized that negative ionization is far from harmless to humans. The analysis of the state of sheep breeding in the Russian Federation is given and this industry in the Rostov region is characterized against this background. The data on the number and productivity of sheep of the main age and sex groups, the production of young animals per one hundred ewes are presented. The characteristic of tribal resources of the country and region is given. The breeding base of the industry in the Russian Federation is represented by 205 breeding organizations, including 3 breeding and genetic centers, 44 breeding plants, 145 breeding reproducers and 13 gene pool farms, which contain 1389.8 thousand sheep (39.0 % of their total number in agricultural organizations and 6.6 % of the sheep population in farms of all categories), including 904.7 thousand Queens (35.0 and 6.2%, respectively). The breeding base of the Rostov region has eleven farms, of which 6 breeding plants for such breeds as the Soviet Merino and salskaya and 5 breeding reproducers. In addition to these breed resources, there are breeding reproducers for the qigai and edilbaev breeds in the region. Total number of breeding sheep in the region is almost 44 thousand. The article indicates main problems in the development of the industry and ways and means to solve them
1215 kb

CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY

abstract 1341710034 issue 134 pp. 404 – 426 29.12.2017 ru 652
A method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
412 kb

CORRECTION OF DYSBIOTIC DISORDERS IN BEES BY USING PROBIOTIC MEDICATIONS

abstract 1582004019 issue 158 pp. 268 – 279 30.04.2020 ru 13
This article provides an overview of the need of the use of probiotic supplements and medications in the diets of insects, in particular bees and their families. We present a list of both Russian and foreign probiotics and their complexes, which are used in beekeeping for the treatment and prevention of various diseases, are shown. Their effectiveness is shown not only for the prevention and treatment of microbial disorders in the gastrointestinal tract of bees due to the manifestation of antagonistic properties in relation to opportunistic and pathogenic microflora, but also the possibility of their use as tools that can increase the immune status, the safety of bee families, and life expectancy. The article shows data on the positive effect of probiotics on the physiological and biochemical parameters of honeybees, activation of metabolic processes in the formation of a fat body, growth of strength and weight of larvae, development of pharyngeal glands, etc.
331 kb

CORRECTION OF HOMEOSTASIS OF THE ORGANISM OF LARGE CATTLE AT THE NODULAR DERMATITIS

abstract 1381804035 issue 138 pp. 149 – 159 30.04.2018 ru 324
We have studied biochemical changes in the body of cattle, sick with nodular dermatitis in the initial stage of the clinical manifestation of the disease. It is proved that in case of disease in animals there is a significant increase in the level of creatinine up to 295.5 mg%. The excess of the norm by 82.1%. In treatment, this indicator was significantly reduced (189.2 mg%), but did not reach the upper limits of the norm (162.4 mg%). In the blood serum of patients, the carotene content was reduced to 0.09 mg%, which was restored to normal three days after the start of treatment (1.0 mg%). The glucose level at the beginning of the disease was 18% below normal and reached the lower limit of the norm 8 hours after the start of treatment. When studying the activity of CKK, it was found that the activity of the enzyme in animals before the introduction of a 5% solution of sodium hydrogencarbonate is 82% higher than the upper limit of the norm. After the treatment, this indicator returned to normal. The inorganic phosphorus index did not reach the physiological norm (2.5 mmol / l) before and after treatment, and remained 11.6% higher (2.9 mmol / l), which indicates a violation of mineral metabolism in sick animals. The level of reserve alkalinity began to recover after 8 hours of observation and reached a minimum limit of 48.91 vol. % CO2. During the entire period of the experimental studies, before and after the administration of the drug, there were no significant changes in the biochemical parameters of total protein, calcium, vitamin E and urea. The nature of the established changes varied within the limits of physiological norms. The use of a 5% solution of sodium hydrogen carbonate in nodular dermatitis increases the effectiveness of symptomatic treatment, reduces the likelihood of complications and stimulates the production of specific antibodies against viruses and bacteria. We have recommended intravenous injection of 5% sodium hydrogencarbonate solution to sick animals at the rate of 1 ml per 1 kg of live weight of the animal three times with an interval of 3 days
263 kb

DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES

abstract 1331709063 issue 133 pp. 823 – 850 30.11.2017 ru 343
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
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