Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Koshchayev Andrey Georgiyevich

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professor

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Kuban State Agrarian University
   

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Articles count: 41

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263 kb

DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES

abstract 1331709063 issue 133 pp. 823 – 850 30.11.2017 ru 804
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
161 kb

DEVELOPMENT OF OPTIMAL WAY OF OBTAINING A HYDROLYZATE OF LACTIC ACID BACTERIA

abstract 1321708121 issue 132 pp. 1465 – 1477 31.10.2017 ru 547
The work was carried out in the scientific-research laboratory of the chair of Biotechnology, Biochemistry and Biophysics of Kuban SAU, the aim of which is the search of optimal conditions of obtaining the hydrolyzates of lactic acid bacteria by thermoacid and enzymatic ways. The pure cultures of microflora of gastrointestinal tract of quails − Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius were the object of researches. Under conducting the thermal acid hydrolysis there were used the same correlations of bacterial mass and water as well as procedures of clearing of hydrolyzates. The variable conditions were рН, temperature and time of hydrolysis. When carrying out the enzymatic hydrolysis in this study there was used the enzyme from the group of muramidase lysozyme. The amount of enzymes and time of carried out hydrolysis were the variable conditions under obtaining the enzymatic hydrolyzates. In the process of researches there were studied: qualitative content of total protein, peptides with М.м. < 1500, protein and peptides with M.m. > 1500, aminoacids and as well as GMDP (glucosaminilmuramilpentapeptide). On the basis of carried out experiments there was stated that according to all components of biologically active substances the thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates by different ways was revealed at destruction of cells Lb. intermedius. Content of GMDP in thermal acid hydrolyzate was higher than in enzymatic on 0,028 g/100 ml (51,8 %)
164 kb

SECONDARY IMMUNODEFICIENCIES OF SMALL PETS AND THEIR CORRECTION USING MURAMYLDIPEPTIDE

abstract 1311707049 issue 131 pp. 559 – 571 29.09.2017 ru 800
The revolutionary discoveries of recent years in immunology has proved that protection from infections and biological aggression leading role played by innate immunity, which leads to the launch and subsequent work of specific adaptive immunity. Today, it is well-known that almost any pathology of the animal affects the immune system and creates an immune imbalance, leading to development of chronic pathological process, frequent relapses and to the development of different kind of complications. Secondary immunodeficiency states are widely distributed and differ in many etiological factors age, nutritional, medicinal, infectious and neoplastic nature, which leads to increased susceptibility of animal to various раthogenic factors, the weakening of the body. Awareness of the role of immunological disorders in the pathogenesis of a number of diseases has led to the need use in veterinary medicines that are aimed to influence on the immune system of the animal at the known molecular mechanism, to combine safety with high clinical efficacy. In this respect, a promising immunomodulators of microbial origin, due to the knowledge of the recognition mechanisms of the innate immune system such as bacteria as a whole, separate structural units, including mini-optimal biologically active fragments. This review is dedicated to one of the immunomodulator of microbial origin – which is an analog of biologically active fragment of muramyldipeptide of bacterial cell walls ‒ glucosaminylmuramildipeptide (GMDP), which is the active substance of registrated in Russia drug with the trade name Glicopin for use in veterinary practice. The drug showed high efficiency when used in cats, dogs, rodents, ferrets with infections of bacterial and viral etiology; cancer; with the aim of improving the natural resistance of the organism of young and old animals; enhance the effectiveness of vaccination in immunocompromised animals; postvaccine reduce complications; reduce stress financial position, such as during transportation and various veterinary treatments; injuries and on-line intervention (for stimulation of repair processes and tissue regeneration); prevention of immunodeficiency in newborn animals; the reduction of postpartum involutional processes in the uterus. Analysis of literature data allows us to consider an important outcome of immunologiс discoveries of recent years is the change of views on the question of the treatment of infectious diseases and the role of drugs as activators of innate immunity, among which an important role is played by a synthetic analogue of muramyldipeptide (GMDP) is a drug with a known molecular mechanism of action
161 kb

BIOLOGICAL FUNCTIONS OF CAROTENOIDS AT CATTLE REPRODUCTION

abstract 1291705080 issue 129 pp. 1113 – 1125 31.05.2017 ru 1013
Carotenoid preparations are quite widely used in a health care, but in the veterinary and animal husbandry, their use is limited. In terms of livestock farms of the Krasnodar region and the Rostov region at the biochemical monitoring studies of the cattle blood the lack of carotene in the serum is revealed from 45 to 100 % of the samples. To study the biological function of carotenoid we used preparations, which are the oily solutions for injections: β-carotene (manufactured by FS 42-3867, which is produced from the biomass culture of the fungus Blakeslea trispora) and lycopene, emitted from plants. The cows in the test groups during the dry period and within two weeks after birth got carotenoid preparation subcutaneously at a dose of 10 ml per animal with an interval of 7 days. As a result, it was found out that the application of carotenoids has the most favorable conditions for the flow of labor and the postnatal period, which are manifested in the decrease in obstetric and gynecological diseases in cows and reduce the period of infertility. In the experimental groups the levels of vitamin A and carotene1,3 times higher (1,26 ± 0,14 μmol/L vs. 0,97 ± 0,09 μmol/L ) 1,8 times (0,34 ± 0 03 mg /% vs. 0.61 ± 0.11 mg /%) respectively in comparison with the parameters of the intact animals at a high level of confidence (P < 0,05). Phagocytic number and percentage of digestion of neutrophils increased by 8,5 %, the difference in the number of B-cells reached 9,1%, T-cells – 6,7%. In a humoral immunity found a significant increase in Ig A. After comparison of the level of lysozyme activity of blood serum was observed its reduction in all animals immediately after birth, but in the experimental groups this process was less pronounced. The use of carotene preparations helps to reduce the concentration of lipid peroxidation products in the body of cows (diene conjugates - by 16,6 %, ketodienes - by 35,7 %, malondialdehyde – by 11,3 %), which creates favorable conditions for the flow of the metabolic processes associated with ensuring the normal childbirth and post-partum involution of the genitals. In the group of cows, receiving the carotenoid lycopene, the dyspepsia morbidity of the newborn calves compared to a control decreased by 26,7 %, bronchopneumonia - by 13,3 %
574 kb

INTENSIFICATION OF THE CULTIVATION PROCESS OF PHYSIOLOGICALLY-ADAPTED LACTOBACILLI AS A BASIS FOR THE CREATION OF BIOLOGICAL PRODUCTS OF MICROBIAL ORIGIN FOR POULTRY BREEDING

abstract 1281704076 issue 128 pp. 1101 – 1114 28.04.2017 ru 634
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
211 kb

EXPERIENCE OF USING NATURAL MINERAL COMPOUNDS FOR METABOLIC DISORDERS IN CATTLE

abstract 1241610069 issue 124 pp. 1065 – 1084 30.12.2016 ru 754
In this study we show the results of the use of natural mineral compounds in metabolic disorders in cattle-breeding complexes of industrial type. According to official data for 2013-2015, based on the biochemical analysis of blood of cattle, that was held in some farms of Samara region, significant metabolic disorders were determined due to decrease in total protein level on 31,5% in average, decrease in alkali reserve – on 26,9% of samples, decrease in calcium concentration – on 40,7%, decrease of the phosphorus – on 8,1%, decrease in carotene – on 81,7% and glucose – on 84,3% of studies. Clinical examination of herd revealed a high level of animals with obvious signs of mineral metabolism disorders (osteodystrophy and rickets of alimentary origin) and comorbidity (nutritional anemia, gastroenteritis, pneumonia, disturbance of protein, carbohydrate and vitamin metabolism, and others). The use of complex natural compounds improves the efficiency of the traditional complex of treatment and prophylaxis, causing an increase in the total concentration of calcium in the blood serum on 10-55%, increase in calcium-phosphorus ratio till 1,27-2,3, increase in annual growth rate of young animals’ body weight on 8,2-44,8%, increase in milk production on 5,9-16,3%. Preparations have an antianemic action, normalize indicators of micronutrient metabolism of proteins and carbohydrates, enhance non-specific resistance factors and prevent the development of non-contagious internal pathology
311 kb

MODELING REGIONAL SYSTEMS OF BREEDING PIGS

abstract 1141510076 issue 114 pp. 1004 – 1020 30.12.2015 ru 1128
The article contains the experience of the development of the methodology and the computer program for calculation of regional and local systems of pigs hybridization at the example of the Rostov region (Russia). Crossing the GP lines for F1 should be organized in multiplier farm, which may be separate farms and to be part of large commercial farms. For the production of F1 in a multiplier farm, we must breed a purebred specialized paternal and a maternal line, selected on the effect of combining ability. For the successful functioning of the system of hybridization, it is necessary to build a genetic pyramid, including breeding and genetic centers (nucleus farm), multiplier farm, reproducing the baseline. The article gives a detailed calculation of sow population of levels of P, GP, GGP for maternal and paternal breeds of pigs. The program uses user-defined parameters of pigs productivity, of the simulated population, and the parameters characterizing the intensity of selection of young animals. To ensure annual production of 1,822 million pigs in the Rostov region it is necessary to provide the availability of brood stock in the amount of 89 thousand heads, 6 800 heads in the structure of grandparent flocks (GP), 730 heads in the structure of the Grand-Grand-parent stock (GGP), excluding sows second maternal and paternal breeds
232 kb

DEVELOPING LOW TEMPERATURE SOURCE LOCAL HEATING FOR PIGLETS

abstract 1081504056 issue 108 pp. 761 – 778 30.04.2015 ru 1098
During the research, we have developed a low-temperature zonal heating panels with an infrared emitter to create a local microclimate for piglets in the suckling period. This heater is lightweight and durable frame in the form of an inverted box size 400 mm x 1800 mm, attached to the wall of the building swivel. Top frame mounted thermostat is connected to a temperature sensor mounted directly on a metal plate with a thickness of 1 mm, painted in black color. In the upper part of the inner carcass layer is a light insulating material, which is located directly under the low-temperature heat source is electric. Study of the optimal size of the heating panel revealed that in view of the limitations associated with the area of the machine and biological characteristics of the optimum size of the sow for 11 piglets are: width – 400 mm; length – 1500 mm (at ventral position) and 1800 mm (lateral position). Comparison of different heating sources for piglets revealed that the use of infrared lamps enables newborn piglets seating area by 55.6%, while in 28 days – by 88.9%. Infrared lamps can not completely meet the recommended specifications as to ensure a more intense heat lamp to be lowered towards the floor surface, which leads to a reduction in the useful area heated. Using as a source of local heating mats and low-temperature panels satisfies pigs in the area of the den in the early suckling period at 138.9 and 200.0%, to weaning, respectively – by 48.5 and 65.5%. Thus, we have developed a low-temperature heating panels contributed to a more uniform distribution of heat in a recreation area of suckling piglets and to provide a comfortable environment at temperatures of 2-3 C lower in comparison with other methods of heating
335 kb

ECONOMIC-BIOLOGICAL FEATURES AND EXTERIOR MAINTENANCE OF YOUNG CATTLE IN THE KRASNODAR REGION

abstract 1051501064 issue 105 pp. 1046 – 1074 30.01.2015 ru 984
In the Krasnodar region there is a unique gene pool of animals both for dairy and meat. To assess the heifers the authors formed five experimental groups of animals, the most common species in the region. The authors studied the main economic, biological and exterior features: live weight, measurements, assessment of the exterior. The studies found that among intensive species with the largest body weight throughout the growing period, in 8-month old Charolais heifers superior Simmental ones at 17.4 kg. In one year old Simmental heifers were 9.2 kg (R˂0,05) ahead of Charolais and in 15 months – 10 kg (R˂0,05). Over the entire period of growth the highest rates of relative growth rates of heifers were obtained by red steppe breed type named Kuban, which had an advantage over the Aberdeen-Angus – 19.1%, Charolais – 5.6%, Hereford – 6.2%, and the difference between them and Simmental weren’t fixed. When growing, the animals become less tall, more stretched, wide and massive. We have noted that during the period of cultivation we had increased indices: stretch (9% – Red Steppe, 13.8% – Charolais, 16.1% – Hereford); chest (5.4% Aberdeen Angus). This reduces the index of long legs of red steppe – 5.6%, 10.9% for Charolais. The study of linear growth of animals confirms that the animal meat breeds of live weight increase was due to the extension of the body and increase the width of the pelvis and chest. Some different facts were observed in heifers of Red Steppe breed. For them, the most characteristic increase in the width of the chest was 1.44 times, as well as increasing the depth of the chest and chest girth behind the shoulders
229 kb

EXPERIENCE AND PROSPECTS OF SEXING SEMEN FOR CATTLE OF DAIRY COWS IN THE KUBAN REGION

abstract 1011407060 issue 101 pp. 953 – 967 30.09.2014 ru 1856
The article is an analysis of the results of three years of work with sexing sperm in one of the two farms of the Kuban region which have implemented this tech-nology
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