Name
Koshchayev Andrey Georgiyevich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
—
Articles count: 41
We have studied biochemical changes in the body of
cattle, sick with nodular dermatitis in the initial stage
of the clinical manifestation of the disease. It is
proved that in case of disease in animals there is a
significant increase in the level of creatinine up to
295.5 mg%. The excess of the norm by 82.1%. In
treatment, this indicator was significantly reduced (189.2 mg%), but did not reach the upper limits of the
norm (162.4 mg%). In the blood serum of patients, the
carotene content was reduced to 0.09 mg%, which
was restored to normal three days after the start of
treatment (1.0 mg%). The glucose level at the
beginning of the disease was 18% below normal and
reached the lower limit of the norm 8 hours after the
start of treatment. When studying the activity of CKK,
it was found that the activity of the enzyme in animals
before the introduction of a 5% solution of sodium
hydrogencarbonate is 82% higher than the upper limit
of the norm. After the treatment, this indicator
returned to normal. The inorganic phosphorus index
did not reach the physiological norm (2.5 mmol / l)
before and after treatment, and remained 11.6%
higher (2.9 mmol / l), which indicates a violation of
mineral metabolism in sick animals. The level of
reserve alkalinity began to recover after 8 hours of
observation and reached a minimum limit of 48.91
vol. % CO2. During the entire period of the
experimental studies, before and after the
administration of the drug, there were no significant
changes in the biochemical parameters of total
protein, calcium, vitamin E and urea. The nature of
the established changes varied within the limits of
physiological norms. The use of a 5% solution of
sodium hydrogen carbonate in nodular dermatitis
increases the effectiveness of symptomatic treatment,
reduces the likelihood of complications and stimulates
the production of specific antibodies against viruses
and bacteria. We have recommended intravenous
injection of 5% sodium hydrogencarbonate solution to
sick animals at the rate of 1 ml per 1 kg of live weight
of the animal three times with an interval of 3 days
In this study we show the results of the use of natural mineral compounds in metabolic disorders in cattle-breeding complexes of industrial type. According to official data for 2013-2015, based on the biochemical analysis of blood of cattle, that was held in some farms of Samara region, significant metabolic disorders were determined due to decrease in total protein level on 31,5% in average, decrease in alkali reserve – on 26,9% of samples, decrease in calcium concentration – on 40,7%, decrease of the phosphorus – on 8,1%, decrease in carotene – on 81,7% and glucose – on 84,3% of studies. Clinical examination of herd revealed a high level of animals with obvious signs of mineral metabolism disorders (osteodystrophy and rickets of alimentary origin) and comorbidity (nutritional anemia, gastroenteritis, pneumonia, disturbance of protein, carbohydrate and vitamin metabolism, and others). The use of complex natural compounds improves the efficiency of the traditional complex of treatment and prophylaxis, causing an increase in the total concentration of calcium in the blood serum on 10-55%, increase in calcium-phosphorus ratio till 1,27-2,3, increase in annual growth rate of young animals’ body weight on 8,2-44,8%, increase in milk production on 5,9-16,3%. Preparations have an antianemic action, normalize indicators of micronutrient metabolism of proteins and carbohydrates, enhance non-specific resistance factors and prevent the development of non-contagious internal pathology
The revolutionary discoveries of recent years in immunology has proved that protection from infections and biological aggression leading role played by innate immunity, which leads to the launch and subsequent work of specific adaptive immunity. Today, it is well-known that almost any pathology of the animal affects the immune system and creates an immune imbalance, leading to development of chronic pathological process, frequent relapses and to the development of different kind of complications. Secondary immunodeficiency states are widely distributed and differ in many etiological factors age, nutritional, medicinal, infectious and neoplastic nature, which leads to increased susceptibility of animal to various раthogenic factors, the weakening of the body. Awareness of the role of immunological disorders in the pathogenesis of a number of diseases has led to the need use in veterinary medicines that are aimed to influence on the immune system of the animal at the known molecular mechanism, to combine safety with high clinical efficacy. In this respect, a promising immunomodulators of microbial origin, due to the knowledge of the recognition mechanisms of the innate immune system such as bacteria as a whole, separate structural units, including mini-optimal biologically active fragments. This review is dedicated to one of the immunomodulator of microbial origin – which is an analog of biologically active fragment of muramyldipeptide of bacterial cell walls ‒ glucosaminylmuramildipeptide (GMDP), which is the active substance of registrated in Russia drug with the trade name Glicopin for use in veterinary practice. The drug showed high efficiency when used in cats, dogs, rodents, ferrets with infections of bacterial and viral etiology; cancer; with the aim of improving the natural resistance of the organism of young and old animals; enhance the effectiveness of vaccination in immunocompromised animals; postvaccine reduce complications; reduce stress financial position, such as during transportation and various veterinary treatments; injuries and on-line intervention (for stimulation of repair processes and tissue regeneration); prevention of immunodeficiency in newborn animals; the reduction of postpartum involutional processes in the uterus. Analysis of literature data allows us to consider an important outcome of immunologiс discoveries of recent years is the change of views on the question of the treatment of infectious diseases and the role of drugs as activators of innate immunity, among which an important role is played by a synthetic analogue of muramyldipeptide (GMDP) is a drug with a known molecular mechanism of action
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
In the Krasnodar region there is a unique gene pool of animals both for dairy and meat. To assess the heifers the authors formed five experimental groups of animals, the most common species in the region. The authors studied the main economic, biological and exterior features: live weight, measurements, assessment of the exterior. The studies found that among intensive species with the largest body weight throughout the growing period, in 8-month old Charolais heifers superior Simmental ones at 17.4 kg. In one year old Simmental heifers were 9.2 kg (R˂0,05) ahead of Charolais and in 15 months – 10 kg (R˂0,05). Over the entire period of growth the highest rates of relative growth rates of heifers were obtained by red steppe breed type named Kuban, which had an advantage over the Aberdeen-Angus – 19.1%, Charolais – 5.6%, Hereford – 6.2%, and the difference between them and Simmental weren’t fixed. When growing, the animals become less tall, more stretched, wide and massive. We have noted that during the period of cultivation we had increased indices: stretch (9% – Red Steppe, 13.8% – Charolais, 16.1% – Hereford); chest (5.4% Aberdeen Angus). This reduces the index of long legs of red steppe – 5.6%, 10.9% for Charolais. The study of linear growth of animals confirms that the animal meat breeds of live weight increase was due to the extension of the body and increase the width of the pelvis and chest. Some different facts were observed in heifers of Red Steppe breed. For them, the most characteristic increase in the width of the chest was 1.44 times, as well as increasing the depth of the chest and chest girth behind the shoulders
Carotenoid preparations are quite widely used in a health care, but in the veterinary and animal husbandry, their use is limited. In terms of livestock farms of the Krasnodar region and the Rostov region at the biochemical monitoring studies of the cattle blood the lack of carotene in the serum is revealed from 45 to 100 % of the samples. To study the biological function of carotenoid we used preparations, which are the oily solutions for injections: β-carotene (manufactured by FS 42-3867, which is produced from the biomass culture of the fungus Blakeslea trispora) and lycopene, emitted from plants. The cows in the test groups during the dry period and within two weeks after birth got carotenoid preparation subcutaneously at a dose of 10 ml per animal with an interval of 7 days. As a result, it was found out that the application of carotenoids has the most favorable conditions for the flow of labor and the postnatal period, which are manifested in the decrease in obstetric and gynecological diseases in cows and reduce the period of infertility. In the experimental groups the levels of vitamin A and carotene1,3 times higher (1,26 ± 0,14 μmol/L vs. 0,97 ± 0,09 μmol/L ) 1,8 times (0,34 ± 0 03 mg /% vs. 0.61 ± 0.11 mg /%) respectively in comparison with the parameters of the intact animals at a high level of confidence (P < 0,05). Phagocytic number and percentage of digestion of neutrophils increased by 8,5 %, the difference in the number of B-cells reached 9,1%, T-cells – 6,7%. In a humoral immunity found a significant increase in Ig A. After comparison of the level of lysozyme activity of blood serum was observed its reduction in all animals immediately after birth, but in the experimental groups this process was less pronounced. The use of carotene preparations helps to reduce the concentration of lipid peroxidation products in the body of cows (diene conjugates - by 16,6 %, ketodienes - by 35,7 %, malondialdehyde – by 11,3 %), which creates favorable conditions for the flow of the metabolic processes associated with ensuring the normal childbirth and post-partum involution of the genitals. In the group of cows, receiving the carotenoid lycopene, the dyspepsia morbidity of the newborn calves compared to a control decreased by 26,7 %, bronchopneumonia - by 13,3 %
During the study of different methods of fractiona-tion of alfalfa juice it was established that the most effective preservative of Lucerne juice is a composi-tion containing 2% of stock culture of lactic acid bacteria, 2% of molasses and 0.3% of benzoic acid, which preserves the functional properties of protein-vitamin feed additive
During the research, we have developed a low-temperature zonal heating panels with an infrared emitter to create a local microclimate for piglets in the suckling period. This heater is lightweight and durable frame in the form of an inverted box size 400 mm x 1800 mm, attached to the wall of the building swivel. Top frame mounted thermostat is connected to a temperature sensor mounted directly on a metal plate with a thickness of 1 mm, painted in black color. In the upper part of the inner carcass layer is a light insulating material, which is located directly under the low-temperature heat source is electric. Study of the optimal size of the heating panel revealed that in view of the limitations associated with the area of the machine and biological characteristics of the optimum size of the sow for 11 piglets are: width – 400 mm; length – 1500 mm (at ventral position) and 1800 mm (lateral position). Comparison of different heating sources for piglets revealed that the use of infrared lamps enables newborn piglets seating area by 55.6%, while in 28 days – by 88.9%. Infrared lamps can not completely meet the recommended specifications as to ensure a more intense heat lamp to be lowered towards the floor surface, which leads to a reduction in the useful area heated. Using as a source of local heating mats and low-temperature panels satisfies pigs in the area of the den in the early suckling period at 138.9 and 200.0%, to weaning, respectively – by 48.5 and 65.5%. Thus, we have developed a low-temperature heating panels contributed to a more uniform distribution of heat in a recreation area of suckling piglets and to provide a comfortable environment at temperatures of 2-3 C lower in comparison with other methods of heating
The article contains the experience of the development
of the methodology and the computer program for
calculation of regional and local systems of pigs
hybridization at the example of the Rostov region
(Russia). Crossing the GP lines for F1 should be
organized in multiplier farm, which may be separate
farms and to be part of large commercial farms. For
the production of F1 in a multiplier farm, we must
breed a purebred specialized paternal and a maternal
line, selected on the effect of combining ability. For
the successful functioning of the system of
hybridization, it is necessary to build a genetic
pyramid, including breeding and genetic centers
(nucleus farm), multiplier farm, reproducing the
baseline. The article gives a detailed calculation of sow
population of levels of P, GP, GGP for maternal and
paternal breeds of pigs. The program uses user-defined
parameters of pigs productivity, of the simulated
population, and the parameters characterizing the
intensity of selection of young animals. To ensure
annual production of 1,822 million pigs in the Rostov
region it is necessary to provide the availability of
brood stock in the amount of 89 thousand heads, 6 800
heads in the structure of grandparent flocks (GP), 730 heads in the structure of the Grand-Grand-parent stock
(GGP), excluding sows second maternal and paternal
breeds
The article provides an overview of the results of experimental works of the authors on the use of different methods of fractionation of Lucerne juice to obtain feed additives