Scientific Journal of KubSAU

The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding

The article presents a comparative assessment of the meat productivity of young cattle of the two most common intensive meat breeds in the Russian Federation (Aberdeen Angus and Hereford) in comparison with the most numerous native in the South of the country domestic breed, which is Kalmyk. Intensive rearing from 9 to 18 months of age was carried out in the conditions of industrial fattening complex on rations not containing juicy forages. In the process of 273-day intensive rearing, in bulls of the tested breeds average daily live weight gains from 1455 to 1526 grams were achieved. During this period, an absolute increase was obtained in Aberdeen-Angus bulls-416.6 kg, in peers of Hereford and Kalmyk breeds-413.6 and 397.2 kg. The highest pre-slaughter live weight and slaughter rates were noted in Aberdeen-Angus bulls, having a yield of a pair carcass of 59.4 % and a slaughter yield of 62.8 %. The meat of Aberdeen-Angus bulls has the highest content of fat and dry matter, but they have less protein, lower pH and moisture binding capacity and a higher loss of meat juice during cooking, compared with the bulls of the Kalmyk breed. It was confirmed that the coefficients of transformation of protein and energy into products are closely related to the intensity of growth and weight of the carcass. The most effective of these indicators were in bulls of Aberdeen-Angus, then Hereford and Kalmyk breeds. A similar pattern between the breeds was manifested in terms of profitability and cost recovery

The article contains the experience of the development of the methodology and the computer program for calculation of regional and local systems of pigs hybridization at the example of the Rostov region (Russia). Crossing the GP lines for F1 should be organized in multiplier farm, which may be separate farms and to be part of large commercial farms. For the production of F1 in a multiplier farm, we must breed a purebred specialized paternal and a maternal line, selected on the effect of combining ability. For the successful functioning of the system of hybridization, it is necessary to build a genetic pyramid, including breeding and genetic centers (nucleus farm), multiplier farm, reproducing the baseline. The article gives a detailed calculation of sow population of levels of P, GP, GGP for maternal and paternal breeds of pigs. The program uses user-defined parameters of pigs productivity, of the simulated population, and the parameters characterizing the intensity of selection of young animals. To ensure annual production of 1,822 million pigs in the Rostov region it is necessary to provide the availability of brood stock in the amount of 89 thousand heads, 6 800 heads in the structure of grandparent flocks (GP), 730 heads in the structure of the Grand-Grand-parent stock (GGP), excluding sows second maternal and paternal breeds

In this work, we have found shortened particle forms of the filamentous bacteriophage Pf, when the lysis of the Pseudomonas aeruginosa PAO1 strain was induced by extracts of bison feces to study the intestinal bacteriophages of this animal. The sizes of the shortened particles from 250 to 950 nm and their thickness 17 nm were determined. The particles had a slightly pointed one end and sometimes a flag on the other extended end. The particles were formed in the process lysis of this strain by other bacteriophages. These lysates also contained a high concentration of vesicles from the pseudomonad membrane. The article discusses the mechanisms of prophage induction in the Pseudomonas aeruginosa PAO1 strain due to phage-phage induction and substances of the investigated extract

The article covers the study of the influence of sodium hypochlorite different concentrations on the quail organism in various schemes of its application

The present review highlights the applications of electro-activated aqueous solutions in biotechnology and the food industry

During experiments on quail it was found that use of Trilaktobaktis probiotic is characterized with the activation of major types of metabolism, including protein –by increase the total protein content (5.17%) and mineral – by increase serum calcium and phosphorus (2.71 and 19.5%, respectively); by decrease of cholesterol content (10.6%). Probiotic stimulates erythro and hematopoiesis in quail: increase of the number of erythrocytes by 2.93% and hemoglobin by 2.57% was marked. Preparation enhances nonspecific resistance by increasing lysozyme and serum bactericidal activity by 47.4% and 149.6%.Besides, use of Trilaktobaktin diets increases digestibility of organic substance (5.74%), raw protein (3.95%) and raw fiber (4.83%). Probiotics use in the diet of quail improves nitrogen digestion by 5.3%, calcium and phosphorus – by 1.4%. Under the influence of probiotics content of lactic acid bacteria and bifidobacteriain chyme of quail increases by 46.2%.Use of Trilaktobaktin quail breeding increases safety and live weight gain by 6.0% and 6.9%, reduces the cost of feeds to produce 1 kg of body weight by 7.4%, and carcass yield reaches 66.4%, providing 100% output of first category carcasses. Use of Trilaktobakt feed additive has a positive effect on the quality of meat products. Thus, carcass output was 57.1% and the amount of fat decreased by 14.4% (pectoral muscles) and 12.7% (leg muscles) relative to the control. At the same time protein content in the thoracic and leg muscles increased by 7.5% and 10.7%

During the study of different methods of fractiona-tion of alfalfa juice it was established that the most effective preservative of Lucerne juice is a composi-tion containing 2% of stock culture of lactic acid bacteria, 2% of molasses and 0.3% of benzoic acid, which preserves the functional properties of protein-vitamin feed additive

In the present article the authors provide an overview of the results of experimental works for the use of electro activated water solutions in agroindustrial complex

The revolutionary discoveries of recent years in immunology has proved that protection from infections and biological aggression leading role played by innate immunity, which leads to the launch and subsequent work of specific adaptive immunity. Today, it is well-known that almost any pathology of the animal affects the immune system and creates an immune imbalance, leading to development of chronic pathological process, frequent relapses and to the development of different kind of complications. Secondary immunodeficiency states are widely distributed and differ in many etiological factors age, nutritional, medicinal, infectious and neoplastic nature, which leads to increased susceptibility of animal to various раthogenic factors, the weakening of the body. Awareness of the role of immunological disorders in the pathogenesis of a number of diseases has led to the need use in veterinary medicines that are aimed to influence on the immune system of the animal at the known molecular mechanism, to combine safety with high clinical efficacy. In this respect, a promising immunomodulators of microbial origin, due to the knowledge of the recognition mechanisms of the innate immune system such as bacteria as a whole, separate structural units, including mini-optimal biologically active fragments. This review is dedicated to one of the immunomodulator of microbial origin – which is an analog of biologically active fragment of muramyldipeptide of bacterial cell walls ‒ glucosaminylmuramildipeptide (GMDP), which is the active substance of registrated in Russia drug with the trade name Glicopin for use in veterinary practice. The drug showed high efficiency when used in cats, dogs, rodents, ferrets with infections of bacterial and viral etiology; cancer; with the aim of improving the natural resistance of the organism of young and old animals; enhance the effectiveness of vaccination in immunocompromised animals; postvaccine reduce complications; reduce stress financial position, such as during transportation and various veterinary treatments; injuries and on-line intervention (for stimulation of repair processes and tissue regeneration); prevention of immunodeficiency in newborn animals; the reduction of postpartum involutional processes in the uterus. Analysis of literature data allows us to consider an important outcome of immunologiс discoveries of recent years is the change of views on the question of the treatment of infectious diseases and the role of drugs as activators of innate immunity, among which an important role is played by a synthetic analogue of muramyldipeptide (GMDP) is a drug with a known molecular mechanism of action