Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Luneva Albina Vladimirovna

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Kuban State Agrarian University
   

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Articles count: 9

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284 kb

BIOLOGICAL VALUE OF PLANT PROTEIN AND VITAMIN SUPPLEMENTS

abstract 0971403088 issue 97 pp. 1245 – 1274 31.03.2014 ru 916
Results of the use of plant protein feed additives containing pumpkin paste and soybean seeds of different varieties for quail are presented in the article. It was established that the use of such additives on the basis of Valens soybean allowed to receive higher growth parameters than groups treated with Vilan soybean additive
235 kb

DEVELOPMENT OF A FUNCTIONAL FEED ADDITIVE

abstract 1151601040 issue 115 pp. 643 – 664 27.01.2016 ru 333
The new feed additives for animals based on milk whey, enriched with sprouting wheat, barley, maize grains and lactic-acid bacterium have been presented in this article. This study explores the possibility of combining the prebiotics ability of milk whey and sprouting grains with feed probiotic attributes of microbe to enhance gut health of animals and digestibility of the feed. Twelve variants of products were tested in the study using the microbial and physical-chemical approaches. All the assays showed high count of microorganism and high content of reducing sugar. The results of investigation indicate that selected feed additive show high quality. An additional point is that it contains useful organic acids (lactic, acetic and propionic acids) and 2,1×109 colony-forming unit of probiotics microorganism that hold the concentration for 4 months. There are not yeast, must, coliform bacteria and Staphylococcus aureus in the developed functional feed product. It contributes to the normalization of the microflora of the gastrointestinal tract of animal, suppression of conditionally pathogenic and putrefactive microflora. The elaborated feed component will help to provide combined feed companies with accessible high-quality raw material
161 kb

DEVELOPMENT OF OPTIMAL WAY OF OBTAINING A HYDROLYZATE OF LACTIC ACID BACTERIA

abstract 1321708121 issue 132 pp. 1465 – 1477 31.10.2017 ru 157
The work was carried out in the scientific-research laboratory of the chair of Biotechnology, Biochemistry and Biophysics of Kuban SAU, the aim of which is the search of optimal conditions of obtaining the hydrolyzates of lactic acid bacteria by thermoacid and enzymatic ways. The pure cultures of microflora of gastrointestinal tract of quails − Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius were the object of researches. Under conducting the thermal acid hydrolysis there were used the same correlations of bacterial mass and water as well as procedures of clearing of hydrolyzates. The variable conditions were рН, temperature and time of hydrolysis. When carrying out the enzymatic hydrolysis in this study there was used the enzyme from the group of muramidase lysozyme. The amount of enzymes and time of carried out hydrolysis were the variable conditions under obtaining the enzymatic hydrolyzates. In the process of researches there were studied: qualitative content of total protein, peptides with М.м. < 1500, protein and peptides with M.m. > 1500, aminoacids and as well as GMDP (glucosaminilmuramilpentapeptide). On the basis of carried out experiments there was stated that according to all components of biologically active substances the thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates by different ways was revealed at destruction of cells Lb. intermedius. Content of GMDP in thermal acid hydrolyzate was higher than in enzymatic on 0,028 g/100 ml (51,8 %)
492 kb

DEVELOPMENT OF PROTEIN ENZYME FEED ADDITIVE FOR POULTRY FARMING

abstract 1121508136 issue 112 pp. 1870 – 1886 30.10.2015 ru 844
The research work was conducted at the Research Laboratory of Biotechnology, Biochemistry, Biophysics Department of Kuban State Agrarian University. The main purpose of this study was to select the most productive type of fungus of a genus of Trichoderma and optimal nutrient medium on basis of a waste of reprocessing of soy for acquire of protein enzyme feed supplement. For selection of a fungus of a genus Trichoderma used 3 types: Trichoderma viride, Trichoderma lignorum, and Trichoderma harsianum. We used 3 types of nutrient media as a carrier for micromycete, which were based on soy okara and the additional sources of reprocessing of raw plant material – husk of a sunflower, husk of wheat (bran) and husk of rice. The results demonstrated the benefits of using the nutrient medium based on soy okara forms and husk of a sunflower fungus to enhance Trichoderma lignorum production
161 kb

EFFECTIVENESS OF PROBIOTIC CULTURES AND THEIR CONSORTIUM ON THE MODELS OF EXPERIMENTAL INTESTINAL DYSBACTERIOSIS

abstract 1602006001 issue 160 pp. 1 – 10 30.06.2020 ru 8
The article demonstrates the results of therapeutic and preventive effectiveness of the use of lactic acid bacteria – Lactobacillus brevis and Lactobacillus parabuchneri in laboratory animals, in particular mice and rats. They were isolated from the gastrointestinal tract of a wild quail in experimental violation of the intestinal microbial background. Artificial imbalance of the intestinal microbiota of experimental animals was caused by the use of the antibiotic. As a result of tests it was established that the application of probiotic cultures improved microbial disorders of the gut, however, the use of their consortium in the probiotic promoted consistent clinical result characterized by an elevated level of representatives of the normal flora (lacto- and bifidobacteria) and reduction of the number of representatives of pathogenic and conditionally pathogenic microbiota, with subsequent achievement of a level characteristic of healthy animals
284 kb

EFFICIENCY OF APPLICATION OF FODDER ADDITIVES FOR PREVENTION OF GASTRO-INTESTINAL DISEASES OF YOUNG CATTLE

abstract 1572003027 issue 157 pp. 368 – 381 31.03.2020 ru 7
The aim of the following research is the studying of the efficacy in probiotic drug Probiolact (0.5% and 1.0%) usage, being in combination with Milkshake supplement. The given complex mixture is meant for medicative-phylactic purposes focused on the reduction of young ones death rate owing to gastro – intestinal diseases when neo-natal period. It is specified that the above-mentioned mixture affects the livestock indices. Thus, the average growth of baseline for the period of 30 days experimenting has been 7.4 kg, whereas in 2 tested groups it accounted for 10.3 kg and 11.6 kg, consequently. Therefore, the best possible daily phylactic doze of Probiolact (1%) and Milkshake mixture demonstrates the positive effect on the pro-cess of bifido- and lactoflora formation in calves’ intestinal tract due to normal flora cells quantity increase. With that, intestinal tract contamination level of disease-producing flora gets lower, and the natural resistance of cell-mediated immunity grows. Thus, two major problems of factory farming (i.e. the reduction of young death rate and surplus guarantee) can be solved with giving calves the mixture to drink, along with the first portions of colostrum, and during the following 30 days
574 kb

INTENSIFICATION OF THE CULTIVATION PROCESS OF PHYSIOLOGICALLY-ADAPTED LACTOBACILLI AS A BASIS FOR THE CREATION OF BIOLOGICAL PRODUCTS OF MICROBIAL ORIGIN FOR POULTRY BREEDING

abstract 1281704076 issue 128 pp. 1101 – 1114 28.04.2017 ru 254
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
165 kb

OPTIMIZATION OF COMPOSITION CULTURE MEDIA, TECHNIQUE OF CONCENTRATING AND FREEZE DRYING CONDITIONS FOR CELL OF LACTOBACILLUS ACIDOPHILUS

abstract 1021408042 issue 102 pp. 681 – 691 31.10.2014 ru 1039
This study has investigated the relationship between culture media, technique of concentrating and freeze drying conditions for the cells of Lactobacillus aci-dophilus. The study has demonstrated that milk whey tomato juice-enriched medium has the high-growth properties. This medium allows ensuring high freeze drying survival of Lactobacillus acidophilus
163 kb

PHARMACOLOGICAL EFFECT OF SODIUM HYPOCHLORITE ON QUAIL ORGANISM

abstract 0901306031 issue 90 pp. 488 – 502 30.06.2013 ru 1191
The article covers the study of the influence of sodium hypochlorite different concentrations on the quail organism in various schemes of its application
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