Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
AGRIS logo UlrichsWeb logo DOAJ logo

Name

Lysenko Yury Andreevich

Scholastic degree

Academic rank

Honorary rank

Organization, job position

Kuban State Agrarian University
   

Web site url

Email


Articles count: 11

Sort by: Date Title Views
202 kb

BIOLOGICAL ACTIVITY OF DIFFERENT SORTS OF HUMIC COMPLEX AND ITS INFLUENCE ON THE GROWTH OF PLANTS

abstract 1101506077 issue 110 pp. 1168 – 1178 30.06.2015 ru 935
The article represents the results of the experiments on biological activity of humits of different sorts. The object of research was humic preparation called Vermistar, which was obtained by vermiculturing, on the basis of brown coal. Humics are group of high-molecular substances which have high physiological activity. Humic substances stimulate all biochemical processes on all the stages of plant growth. They change the permeability of the cell membranes, increase activity of ferments, amount of chlorophyll and productivity of photosynthesis. Also humits are not toxic; they don’t have carcinogen and mutagenic effects, so we have a chance of getting environmentally friendly products. The experiment results show high biological activity of humic preparations. Usage of humic stimulates germination, the accumulation of vegetative mass, weight gain. The frequency of shoot has been increased by 17-32%. Height of shoots, roots and vegetative mass has also been increased
412 kb

CORRECTION OF DYSBIOTIC DISORDERS IN BEES BY USING PROBIOTIC MEDICATIONS

abstract 1582004019 issue 158 pp. 268 – 279 30.04.2020 ru 20
This article provides an overview of the need of the use of probiotic supplements and medications in the diets of insects, in particular bees and their families. We present a list of both Russian and foreign probiotics and their complexes, which are used in beekeeping for the treatment and prevention of various diseases, are shown. Their effectiveness is shown not only for the prevention and treatment of microbial disorders in the gastrointestinal tract of bees due to the manifestation of antagonistic properties in relation to opportunistic and pathogenic microflora, but also the possibility of their use as tools that can increase the immune status, the safety of bee families, and life expectancy. The article shows data on the positive effect of probiotics on the physiological and biochemical parameters of honeybees, activation of metabolic processes in the formation of a fat body, growth of strength and weight of larvae, development of pharyngeal glands, etc.
197 kb

DEVELOPMENT AND EMPLOYMENT OF A NEW PROBIOTIC FODDER ADDITIVE ON THE BASIS OF FUNCTIONAL MICROFLORA IN A RECEIPT OF FORMULA FEED FOR A QUAIL

abstract 0911307073 issue 91 pp. 1050 – 1069 30.09.2013 ru 1005
The article brings to light a research dedicated to the influence of a new probiotic fodder additive “Promomix” and its tradable counterpart “Probiolact” on a quail organism
235 kb

DEVELOPMENT OF A FUNCTIONAL FEED ADDITIVE

abstract 1151601040 issue 115 pp. 643 – 664 27.01.2016 ru 348
The new feed additives for animals based on milk whey, enriched with sprouting wheat, barley, maize grains and lactic-acid bacterium have been presented in this article. This study explores the possibility of combining the prebiotics ability of milk whey and sprouting grains with feed probiotic attributes of microbe to enhance gut health of animals and digestibility of the feed. Twelve variants of products were tested in the study using the microbial and physical-chemical approaches. All the assays showed high count of microorganism and high content of reducing sugar. The results of investigation indicate that selected feed additive show high quality. An additional point is that it contains useful organic acids (lactic, acetic and propionic acids) and 2,1×109 colony-forming unit of probiotics microorganism that hold the concentration for 4 months. There are not yeast, must, coliform bacteria and Staphylococcus aureus in the developed functional feed product. It contributes to the normalization of the microflora of the gastrointestinal tract of animal, suppression of conditionally pathogenic and putrefactive microflora. The elaborated feed component will help to provide combined feed companies with accessible high-quality raw material
161 kb

DEVELOPMENT OF OPTIMAL WAY OF OBTAINING A HYDROLYZATE OF LACTIC ACID BACTERIA

abstract 1321708121 issue 132 pp. 1465 – 1477 31.10.2017 ru 170
The work was carried out in the scientific-research laboratory of the chair of Biotechnology, Biochemistry and Biophysics of Kuban SAU, the aim of which is the search of optimal conditions of obtaining the hydrolyzates of lactic acid bacteria by thermoacid and enzymatic ways. The pure cultures of microflora of gastrointestinal tract of quails − Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius were the object of researches. Under conducting the thermal acid hydrolysis there were used the same correlations of bacterial mass and water as well as procedures of clearing of hydrolyzates. The variable conditions were рН, temperature and time of hydrolysis. When carrying out the enzymatic hydrolysis in this study there was used the enzyme from the group of muramidase lysozyme. The amount of enzymes and time of carried out hydrolysis were the variable conditions under obtaining the enzymatic hydrolyzates. In the process of researches there were studied: qualitative content of total protein, peptides with М.м. < 1500, protein and peptides with M.m. > 1500, aminoacids and as well as GMDP (glucosaminilmuramilpentapeptide). On the basis of carried out experiments there was stated that according to all components of biologically active substances the thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates by different ways was revealed at destruction of cells Lb. intermedius. Content of GMDP in thermal acid hydrolyzate was higher than in enzymatic on 0,028 g/100 ml (51,8 %)
492 kb

DEVELOPMENT OF PROTEIN ENZYME FEED ADDITIVE FOR POULTRY FARMING

abstract 1121508136 issue 112 pp. 1870 – 1886 30.10.2015 ru 863
The research work was conducted at the Research Laboratory of Biotechnology, Biochemistry, Biophysics Department of Kuban State Agrarian University. The main purpose of this study was to select the most productive type of fungus of a genus of Trichoderma and optimal nutrient medium on basis of a waste of reprocessing of soy for acquire of protein enzyme feed supplement. For selection of a fungus of a genus Trichoderma used 3 types: Trichoderma viride, Trichoderma lignorum, and Trichoderma harsianum. We used 3 types of nutrient media as a carrier for micromycete, which were based on soy okara and the additional sources of reprocessing of raw plant material – husk of a sunflower, husk of wheat (bran) and husk of rice. The results demonstrated the benefits of using the nutrient medium based on soy okara forms and husk of a sunflower fungus to enhance Trichoderma lignorum production
284 kb

EFFICIENCY OF APPLICATION OF FODDER ADDITIVES FOR PREVENTION OF GASTRO-INTESTINAL DISEASES OF YOUNG CATTLE

abstract 1572003027 issue 157 pp. 368 – 381 31.03.2020 ru 14
The aim of the following research is the studying of the efficacy in probiotic drug Probiolact (0.5% and 1.0%) usage, being in combination with Milkshake supplement. The given complex mixture is meant for medicative-phylactic purposes focused on the reduction of young ones death rate owing to gastro – intestinal diseases when neo-natal period. It is specified that the above-mentioned mixture affects the livestock indices. Thus, the average growth of baseline for the period of 30 days experimenting has been 7.4 kg, whereas in 2 tested groups it accounted for 10.3 kg and 11.6 kg, consequently. Therefore, the best possible daily phylactic doze of Probiolact (1%) and Milkshake mixture demonstrates the positive effect on the pro-cess of bifido- and lactoflora formation in calves’ intestinal tract due to normal flora cells quantity increase. With that, intestinal tract contamination level of disease-producing flora gets lower, and the natural resistance of cell-mediated immunity grows. Thus, two major problems of factory farming (i.e. the reduction of young death rate and surplus guarantee) can be solved with giving calves the mixture to drink, along with the first portions of colostrum, and during the following 30 days
301 kb

FEATURES OF CULTIVATION OF BROILERS OF HUBBARD REDBRO CROSS IN FARMS FOR “ORGANIC” MEAT

abstract 1201606105 issue 120 pp. 1576 – 1605 30.06.2016 ru 311
This article is devoted to studying the influence of conditions of keeping and feedings on broilers of Hubbard RedBro cross, and the quality of the received products in case of application of floor and cellular content in the conditions of a farm. It was found that when receiving a feed of its own production with the use of feed ingredients grown in farms without pesticides, recorded a statistically significant reduction in the potentially dangerous health animal substances. As compared with a factory forage therein reduced pesticide content by 14 times, mercury and arsenic is 24 times, 5 times cadmium, lead 10 times. Results of studying of economic indicators of cultivation of broilers of cross Hubbard RedBro and also chemical composition and quality of carcasses demonstrated that the essential difference between a floor and cellular condition of keeping is not established, however use of a diet on the basis of what forages promoted statistically reliable decrease in concentration of toxic metals in muscles of a bird of experienced groups. As a result it was found that use of the researched compound feed in diets of broilers raised indicators of biosafety and provided ecologically safe (“organic”) meat products of poultry farming
574 kb

INTENSIFICATION OF THE CULTIVATION PROCESS OF PHYSIOLOGICALLY-ADAPTED LACTOBACILLI AS A BASIS FOR THE CREATION OF BIOLOGICAL PRODUCTS OF MICROBIAL ORIGIN FOR POULTRY BREEDING

abstract 1281704076 issue 128 pp. 1101 – 1114 28.04.2017 ru 273
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
165 kb

OPTIMIZATION OF COMPOSITION CULTURE MEDIA, TECHNIQUE OF CONCENTRATING AND FREEZE DRYING CONDITIONS FOR CELL OF LACTOBACILLUS ACIDOPHILUS

abstract 1021408042 issue 102 pp. 681 – 691 31.10.2014 ru 1056
This study has investigated the relationship between culture media, technique of concentrating and freeze drying conditions for the cells of Lactobacillus aci-dophilus. The study has demonstrated that milk whey tomato juice-enriched medium has the high-growth properties. This medium allows ensuring high freeze drying survival of Lactobacillus acidophilus
.