Name
Grankina Anastasiya Olegovna
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
South Federal University
Web site url
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Articles count: 5
The aim of the study is to compare the traditional
clinicopathologic factors and prognosis of cell cycle
parameters, such as ploidy, cell cycle kinetics, as well
as the study of DNA content in the tumor tissue of
patients with esophageal cancer. Cancer of the
esophagus - a very aggressive malignant disease, most
commonly manifested impaired swallowing. As a rule,
this type of cancer pathology is characterized by
unfavorable outcome. Ploidy and cell proliferation
activity, defined by the content of the DNA of the
tumor - synthesizing cells are one of the important
indicators of the growth rate of malignancy and
prognosis. The study was performed on tumor tissue
fragments of the 30 patients operated on for cancer of
the esophagus. DNA study - conducted in the open cell
material aftercare. DNA cell content was determined
by flow cytometry BD FACSCanto II. By studying the
DNA-cytometric parameters, we found the prevalence of aneuploid tumors in 66.7%. It was shown that DNAcytometric
parameters are independent of gender.
Major weight of diploid and aneuploid cell tumors of
the esophagus was in G0 / 1 phase of the cell cycle.
Comparative analysis of DNA - cytometric parameters
and the degree of differentiation of esophageal tumor
revealed a prevalence of aneuploid tumors in all the
test cases. content distribution pattern of tumor cells
was found in the G0 / G1-, G2 + M -, S - phase of the
cell cycle at different stages of the disease
Prostate cancer (PC) is one of the most frequently
malignant neoplasms in the worldwide male
population. Back in the 90's began a promising noninvasive
method of high-intensity focused ultrasound
(HIFU) as an alternative to radical prostatectomy for
the treatment of localized prostate cancer was used.
Information about the destructive histological effect by
HIFU has been known since the 1930, however, the
clinical implementation of this technology due to the
absence at the time visualization capabilities for
monitoring during the procedure has been postponed.
In this paper, an overview of published data on the
HIFU clinical application in prostate cancer as a major,
"saving" and focal therapy. Examples of a favorable
combination of this method are also provided with
pharmacological agents. Based on the analysis of
clinical data, we can say that HIFU is a good
alternative minimally invasive therapeutic modality for
patients with prostate cancer who are not candidates
for radical prostatectomy. Applying of HIFU is
possible as a treatment option with local recurrence
after external radiotherapy, with careful selection of
patients is important, depending on prognostic factors
Study was performed on 12 samples of astrocytic human brain tumors, which differ by the level of malignancy and histological indications. The study was conducted with the method of immunohistochemistry using a commercially available antibody anti-connexin-43 (Cx 43), a prior test of
which was carried out on myocardium tissue of rats (positive control). The study showed that with the increasing of malignant level of astrocytomas, there was marked the decreased level of connexin-43 expression of, which is apparently to be associated with the destruction of intercellular gap junctions in tumor tissue
Mortality from malignant diseases is directly linked to
early metastasis and relapse. It is now possible to
identify individual tumor cells in bone marrow
biopsies and peripheral blood. Colorectal cancer is one
of the leading places in the structure of cancer
incidence and mortality, both in Russia and around the
world. Studies have shown that after surgery the ability
of tumor cells to the dissemination and implantation
increases. The work contains 30 patients with the
verified colorectal cancer with metastasis to the liver.
To determine the CSC we have applied an analysis
technology in CellSearch System â„¢ (Janssen
Diagnostics, LLC). Our study of the number and
frequency of detection of circulating tumor cells in
peripheral blood of patients and control group
demonstrates the reduction in the number of CTCs
during laparoscopic surgery and changing the
dynamics of the level of tumor cells in the form of
reduction from the stage of the mobilization of the drug due to minimally invasive access, minimizing
manipulation in the projection of the tumor and as a
consequence, preventing the release into the blood
stream of single tumor cells
Recently, much attention is paid to research the role of cell-cell interactions by gap junctions in the process of malignant transformation and mechanisms of antitumor resistance. Meanwhile, the greatest interest is astrocytic tumors. Depending on the degree of malignancy, astrocytomas are divided into: pilocytic astrocytoma (Grade I), subependymal giant cell astrocytoma (Grade I), pleomorphic xanthoastrocytoma (Grade II), diffuse astrocytoma (Grade II), anaplastic astrocytoma (Grade III), glioblastoma (Grade IV ) gliomatosis cerebri (Grade IV). Information of literature devoted to astrocytic tumors (gliomas) - the most common brain tumor in large part obtained in studies in cell cultures and different contradictions. Along with data on the reduction of glial tumors cells communicability through GJ, there is evidence of an opposite character - a functionally active GJ in gliomas and inhibition of tumor growth by reducing intercellular communicability by GJ. However, up to now there have been no studies of the effect and function of hemichannels in cancer cells, which would provide detailed information on: 1) the characteristic of presence and relative abundance of hemichannels in cancer cells; 2) evaluation of absorption / release of hemichannels mediated molecules in tumor cells than in non-tumor cells; 3) functional consequences of activation and blocking of hemichannels in tumor cells and 4) the prognostic value of the expression / activation of hemichannels in human malignancies