Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Zvyagin Andrey Sergeyevich

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Kuban State Agrarian University
   

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Articles count: 14

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161 kb

BIOMETRICAL ESTIMATATION OF THE POLYMORFISM OF GRAPE GROUP OF PINOT AND RIESLING BY MORFOLOGICAL CHARACTERISTICS OF THE MIDDLE PART OF THE TREE

abstract 0520908001 issue 52 pp. 1 – 14 30.10.2009 ru 1977
The polymorphism is the diversity of varieties in ampelografy - the presence simultaneous permanent in the population of two or several phenotypic and genotype differing groups of plants of one variety – i.e. clones. In viticulture the isogenic population is usually called “clone” and variety as the total of clones or forms – i.e. it is heterogenic. Polymorphism of varieties has big practical sense, as varieties-clones have the harmonic complementary adaptive ampelocenosis and higher economical effect for production. In the article the polymorphism of two heterogenic population - the group Pinot and Riesling, which are the best specimen of French and German viticulture is described on the phenotypic level of leaves
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CLONE SELECTION – THE MODERN BASE FOR RAISING GRAPE PRODUCTION

abstract 0510907019 issue 51 pp. 392 – 416 28.09.2009 ru 2213
The clone selection has very outstanding position behind the actual problems of modern viticulture in the world and in our country. This problem usually has the primary importance behind the scientist who works with grape in all the countries such as USA, German, French, Spain, Italy, Hungary, Bulgaria, Moldavia, Ukraine and etc. In our country last years some positions becomes solving in the plane of scientific research work. Consequently, in the article the history and the situation of clone selection of grape on the Eurasian continent is widely covered.
237 kb

EXTRACTION OF DNA FROM HERBARIUM LEAVES VITIS VINIFERA L.

abstract 0581004022 issue 58 pp. 336 – 347 28.04.2010 ru 1738
Two methods were used for DNA extraction from herbarium leaves of two subspecies: V. vinifera subsp. sativa D.C. и Vitis vinifera ssp. silvestris Gmel. DNA extraction is difficult from herbarium leaves plants because of the presence of metabolites that interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning. The quality of DNA was checked on a spectrophotometer by measuring the level of absorption at A260/280 nm. The results showed the successful extraction from mature and dried leaves of DNA when using the СТАB-method №2. Another method hasn’t shown satisfactory results in amplification of DNA
355 kb

EXTRACTION OF DNA FROM LEAVES OF VITIS VINIFERA L.

abstract 0601006036 issue 60 pp. 571 – 587 29.06.2010 ru 1565
Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
4445 kb

GENOTYPING OF NEW PERSPECTIVE WINE GRAPES WITH USING MICROSATELLITE MARKERS

abstract 0981404010 issue 98 pp. 137 – 155 30.04.2014 ru 1212
In this article we have described our work on genotyping of new ten productive wine grape protoclones
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GENOTYPING OF THREE NEW PRODUCTIVE WINE VARIETIES WITH USING MICROSATELLITE MARKERS

abstract 0981404077 issue 98 pp. 1073 – 1087 30.04.2014 ru 1259
In this article we have described a study of genotyping of new three productive wine grapes protoclones: Rhine Rieslin, Verdot black and Johanniter
1347 kb

NOVATION OF VITICULTURE IN RUSSIA 4. DEVELOPMENT OF CLONE SELECTION IN VITICULTURE

abstract 0540910009 issue 54 pp. 160 – 181 21.12.2009 ru 2288
The analyze of the genetic relationship or diversity between clones was made by using the microsatellites in the groups with black berries: Cabernet-Sauvignon and Merlot, and with white berries: Muscat white, Riesling and Pinot. It was possible to approve the objective evidence on the polymorphism between these forms during this analyze. These results become the rationale for the selection, breeding and making registration of these protoclones of four above-named varieties groups in the varieties-clones for state research: Cabernek, Cabernet Fanogoriisky, Cleret temrukskii, Merlok, Pinot white, Rieslinalk, Riesling Anapa, Riesling Fanogoriisky, Muscat Temruk, Shardonek and others. These varieties-clones are accepted by State commission of Russia, they will enrich the viticulture assortment of Anapa-Tamanskay zones of Kuban, therefore Russia too
493 kb

STRUCTURAL AND PHYLOGENETIC ANALYSIS OF VVMYBA1, VVMYBA2 AND VVMYBA3 GRAPEVINE GENES

abstract 1341710026 issue 134 pp. 303 – 327 29.12.2017 ru 96
The article presents the structural and phylogenetic analysis of VIT_02s0033g00410, VIT_02s0033g00390 and VIT_02s0033g00450 genes of the grapevine genome and the closely related orthologous gene MYB114 of the Arabidopsis genome. These genes are responsible for the biosynthesis of anthocyanin in the organs of model plants and are of interest not only for practical production and breeding, but also for fundamental research. These genes were analyzed for GCcomposition of nucleotides, the presence of cisregulatory elements and promoter regions. DNA and protein sequences were aligned to look for similar elements, which allowed further analysis of the ultraconservative domains of four genes. Based on the results of search and identification of the conservative regions, a cluster tree was constructed, which made it possible to identify the separation of gene sidelines from, presumably, the main one. At the same time, the construction of consensus trees based on DNA and protein sequences revealed their absolute similarity. "The Minimal Evolution Tree" allowed calculating the approximate dates of the appearance of the mutations and the divergence times of the gene branches between each other. At the same time, the appearance of the Vitis genus and its separation from the Rosales was taken as the time first divergence point. In the end, homologous metabolic pathways were searched between grapevine and Arabidopsis, which revealed the presence of homologous proteins in the grape proteome. In this turn, it already confirms the existence of similar biosynthetic pathways and, as a consequence, interactions such as "DNA-protein" and "protein-protein"
182 kb

STUDY OF INDIGENEOUS RUSSIAN GRAPE VARIETIES USING MICROSATELLITE MARKERS

abstract 0881304013 issue 88 pp. 196 – 205 30.04.2013 ru 891
The analysis of genetic polymorphisms of 12 autochthonous grape varieties grown in the National ampelographic collection of Russia (Anapa district of the Krasnodar region) through the study of allelic diversity at six microsatellite loci: VRZAG79, VVMD5, VVMD7, VVMD27, VRZAG62, VVS2 has been done. We have found that all native varieties have a unique set of allele. The assessment of genetic relationships varieties has been performed using cluster analysis. Data for DNA certification of the investigated genotypes of the grapes has also been obtained in the article
172 kb

TECNOLOGY OF CLONE SELECTION WITH THE USE OF MOLECULAR MARKERS

abstract 0681104014 issue 68 pp. 160 – 176 28.04.2011 ru 1374
The analyze of the genetic relationship or diversity between clones was made by using the microsatellites in the groups with black berries: Cabernet-Sauvignon and Merlot, and with white berries: Riesling and Pinot. These results become the rationale for the new technology of clone selection, breeding and making registration of these clones of four above-named varieties groups in the varieties-clones for state research: Cabernek, Cabernet Fanogoriisky, Merlok, Pinot white, Rieslinalk, Riesling Anapa and Riesling Fanogoriisky. These varieties-clones are accepted by State commission of Russia, they will enrich the viticulture assortment of Anapa-Tamanskay zone of Kuban, therefore Russia
.