Name
Salfetnikov Anatoliy Alekseevich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
—
Articles count: 17
100 years ago, on June 4, 1920, 32-year-old Professor of the Saratov University Nikolai Ivanovich Vavilov (1887-1943) first reported at the III all-Russian selection Congress at the University of Saratov on his discovery of homological series in the study of parallelisms in the phenomena of hereditary variability by analogy with homological series of organic compounds. This discovery in genetics received the rank of law, the only one after the laws of G. Mendel. This major study was a further development of the genetic idea of C. Darwin on the origin of species. It showed the ways in which close species and genera of plants have a parallel formative process, because the crucial in the process of evolutionary development of living organisms – first of all, their genetic features. In cases where the development of a trait requires the joint and consistent action of many genes, the occurrence of homological series is inevitable, and this does not contradict the random variability of C. Darwin. In addition to its great genetic significance as a law of evolution, the law of homological series in hereditary variability is of great importance for botanists, plant breeders and breeders: it not only determines the place of each form in the plant world, but can also indicate to the breeder possible directions in his practical work. According to a number of geneticists and breeders, if G. Mendel discovered the rules of heredity, then N. I. Vavilov discovered the rules of variability
Due to the wide spread of winter barley varieties with
different Phytopathologic characteristics, a serious
threat to the crop is dwarf rust caused by a pathogenic
fungi of the Puccinia. The article presents the results of
phytosanitary studies conducted in different agroclimatic
zones of the North Caucasus region for the
period from 2013 to 2015. Electronic maps of levels of
development and distribution of dwarf rust are based on
phytosanitary inspection. On the experimental field
called «Kuban» as well as rust nursery of VNIIBZR we
have performed an immunological evaluation of the
sustainability of winter barley accessions to the
pathogen of the dwarf rust on natural and artificial
infectious backgrounds in the period from 2013 to
2015. The article reflects the objective characteristics of
varieties and lines of winter barley with a relative
stability to the North Caucasian population Puccinia
hordei. The most promising varieties and lines of winter
barley of the KubGAU breeding were selected during
operation. These varieties and lines are highly resistant
to rust and dwarf, they also have other valuable
features. The article presents information on the best
yield varieties and lines of winter barley as well as on
gross yield and sowing area
The review examines research unusual properties of
RNA. RNA has the ability to act as both genes and
enzymes (ribozymes). This property could offer a
way around the «chicken-and-egg» problem: genes
require enzymes; enzymes require genes.
Furthermore, RNA can be transcribed into DNA, in
reverse of the normal process of transcription. These
facts are reasons to consider that the RNA world
could be the original pathway to cells. The general
notion of an «RNA World» is that, in the early
development of life on the Earth, genetic continuity
was assured by the replication of RNA and
genetically encoded proteins were not involved as
catalysts. There is now strong evidence indicating
that an RNA World did indeed exist before DNAand
protein-based life. RNA has multiple functions.
Among these, "messenger RNA" carries genetic
information from DNA to protein formation. RNA is
often a single-stranded spiral, but also exists in
double-stranded form. In 1998, Craig Mello and
Andrew Fire discovered through their studies of the
roundworm C. elegans a phenomenon dubbed "RNA
interference". In this phenomenon, double-stranded
RNA blocks messenger RNA so that certain genetic
information is not converted during protein
formation. This "silences" these genes, i.e. renders
them inactive. The phenomenon plays an important
regulatory role within a genome. Recent years have
been perhaps the most fruitful period yet in terms of
research in the area of mRNA stability (Phenomena:
Gene Silencing; RNA interference; Identity of
mRNA decay in vivo and in vitro). The elaboration
of new methods in biothechnology have been
presented
The differential stability of mRNA is an important
mechanism for posttranscriptional regulation of gene
expression in eukaryotes. Messenger RNA stability is
controlled by specific genes and growth conditions.
The review examines the theoretical possibility of
mRNA stabilization in vivo as a consequence of
replacing carbon atoms (C) or phosphorus (P),
composed of nucleic acid on the silicon (Si) atom.
During isolation of poly-(A)+mRNA from plant
tissues by the two-cycle affinity chromatography on
poly-(U)-Sepharose, regular changes in poly-
(A)++mRNA yield were observed. The changes
varied both with the plant genotype and growth
conditions. Celite treatment of heated and unheated
total RNA preparations from developing corn kernels
and from green and etiolated wheat seedlings.
Messenger RNA that differed in the length of poly-
(A)-sequences was used for hybridization. It is
evident that a reduction of poly-(A)-length causes
alterations in spatial structure of mRNA, and
associated proteins and cations Mg++ become
accessible to celite absorption. Heating promotes
melting of secondary structure, already initiated, and
increases the efficiency of mRNA stabilization by
celite. Interpreting the facts interact celite with mRNA
in vitro and stabilization of mRNA in vivo by
cycloheximide with a modern point of view can be considered with the position research of the
phenomenon of RNA interference
The problem of increasing resistance of winter
barley to adverse conditions of winter is the most
problem in the selection area of this crop. The main
component of this complex trait is the frost
resistance. Laboratory methods of assessment of
frost resistance are important for breeding of winter
barley. These methods can effectively replace more
costly method of direct freezing in the freezers.
Magnesium cations (Mg++) are essential
components of the protein-synthesizing system of
germinating seeds. Magnesium cations determine
the stability and translational activity of mRNA and
rRNA. Trilon B is usually used for in vitro banding
of magnesium cations in area of biochemistry of the
nucleic acids. It was very interesting to verify the
Trilon B influence to seed germination of winter
barley varieties by experiment. Experiments
showed: actinomycin D – the inhibitor of RNA
synthesis – didn’t show varieties specific effect to
seed growth of winter barley, while Trilon B had a
varieties specific impact to length coleoptiles and
roots. Research was carried out on etiolated
seedlings at temperatures from 22 to 28oC, and different concentrations of Trilon B - 1,6×10-3М,
2,4×10-3М and 3,2×10-3М. The roots of 3-days old
seedlings were more sensitive to Trilon B influence.
It is shown the varieties of Russian origin were
naturally reacted to Trilon B: the higher the frost
resistance of variety, the more resistant variety to
the action of various concentrations of Trilon B at
different temperatures. The most optimum
concentration was 3,2 × 10-3М at 22-25oC.
Varieties by foreign origin deviated from this
pattern. It is interesting to research the genetic
essence of these differences. It will be helpful for
optimization of varieties differentiation conditions
to create a laboratory method of estimate of frost
resistance of winter barley. The new method will be
most efficient compared the direct freezing method
This overview and theoretical article deals with the consideration of hypothetical possibilities for the development of molecular-kinetic markers of agricultural plants allowing to quantify the effect of genotype-environment interaction on the basis of stability studies of mRNA. The development in view is based on the results of studies of the mRNA decay identity in vivo and in vitro (the ommp system), as well as the phenomenon of RNA interference (RNAi), widely studied in plants. The ommp system has allowed to establish the relationship of the cultivar-specific growth reaction to the effects of low positive temperatures, dehydration, salinity, illumination and biologically active substances with the stability of the total and a number of gene-specific mRNAs of green and etiolated seedlings of winter soft wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.). Similar studies of mRNA stability have been conducted on ripening grains of maize (Zea mays L.), with a particular result of the in vivo and in vitro decay identity confirmed for major mRNAs of stored proteins, 19 and 22 kDa zeins, of normal maize and mutant according to the regulatory gene opaque-2, changing the amount and stability of zein mRNA in the maturing grain of high-lysine maize. Regulatory response of the organism through RNAi is also multiple and includes neutralization of viral and bacterial infections, reaction to pathogens and biologically active substances, circadian rhythms, water stress, hypoxia, mechanical stress, mineral nutrition, salt stress and temperature changes. Unfavorable environmental influences result in an increase or decrease in the expression of certain microRNAs (miRNAs). The change in mRNA stability is an important component of the gene expression regulation system in eukaryotic cells. The main determinants of mRNA stability are in the 3'-untranslated region. It is the It is the (U)nА sequence and the degree of polyadenylation of mRNA, i.e. length of its terminal homonucleotide chain. It is to this region that mRNA molecules are complementary to miRNA. The most important component, which largely determines the regularity of the genotype-environment interaction, is the polyadenyl sequence at the 3 'end of the mRNA. Its length depends both on the genotype and on the environmental conditions. There is evidence that polyadenylation degree of mRNA determines the secondary structure of the molecule. As is known, deadenylation of mRNA reduces its lifetime, and when the poly-A-tail reaches several dozens of nucleotides, an explosive disintegration of the mRNA molecule occurs. Consequently, in a living cell the following mRNA decomposition scheme appears to be logical: shortening the poly-A-tail of the mRNA opens the sites of miRNA interaction with the 3'-non-coding region of the mRNA molecule, which causes its degradation. Thus, it is safe to assume that the interaction of mRNA and miRNA in the ommp system is the underlying process for molecular-kinetic markers under development
The review examines the recent years’ data
pertaining to the interaction of plants with
nanoparticles of noble metals (gold and silver) and
with nanoparticles of magnesium as an element that
has a central role in the organization of the cellular
protein-synthesizing apparatus. Information is
presented about the effect of gold and silver
nanoparticles on plants and on possible nanoparticle
phytotoxicity. It is shown that magnesium
nanoparticles, as well as those of noble metals,
produce various effects on the growth and
productivity of plants, enabling the arsenal of
biologically active substances to be expanded.
Analysis is made of the involvement of magnesium
nanoparticles in cryobiological reaction of the
regular breakdown of plant RNA under conditions of ultra low temperatures, which is important not
only for the further developments of views of the
origin of life ob Earth but also for the purposes of
designing new markers for the analysis of the
biological peculiarities of agricultural crops on the
basis of studies of magnesium in the long-lived
RNA of their mature seeds. The presented critical
analysis of the literature data, which are often
incomplete and contradictory, suggest that a
coordinated research program is needed that would
detect correlation between particle parameters,
experimental design, and observed biological
effects
The article brings the results of three-year studies of the
influence of the periods of sowing on productivity of
new varieties and lines of winter barley
In the review article we present a comparative analysis of frost resistance of varieties of winter barley and sunflower (Helianthus annuus L.) by the results of freezing in the refrigerating chambers and by the hygroscopicity of mature grain. On the example of a number of varieties of both cultures, it is shown that the higher the frost resistance of the variety, the smaller the volume of the supernatant can be obtained by extracting the press cake with a solution containing magnesium cations. It is assumed that increased hygroscopicity of winter barley and sunflower press cake is associated with a relatively high content of so - called "water-soluble starch" - β-glucan polysaccharides in its grain. The possibility of regulation of the degree of hygroscopicity under the influence of potassium (KNO3), zinc (ZnSO4) and gibberellic acid (GA3) was studied on a number of sunflower varieties. It was shown that treatment with a solution of potassium (a concentration of 50 ppm KNO3) of sunflower plants at the stage of 4 leaves leads to a significant increase in hygroscopicity of grain and hardiness of plants, little effect was given treatment with zinc (30 ppm ZnSO4) and a lesser effect was observed when treating plants gibberellovaja acid (20 ppm). Potassium treatment significantly intensified the biosynthesis of free Proline, phenolic compounds and soluble proteins. When processing zinc significantly increased the content of carbohydrates in sunflower plants. Hygroscopicity showed a significant correlation with the content of free Proline (R2=0.621), phenolic compounds (R2=0.907), total carbohydrates (R2=0.673) and soluble proteins (R2=0.708). It is assumed that the treatment of plants with potassium and zinc plays a key role in increasing the hygroscopicity of grain. The results of comparative studies of frost resistance on the degree of survival of plants during freezing in cold rooms and the degree of hygroscopicity of mature grain showed that these two methods of assessing frost resistance give very close data. At the same time, in terms of simplicity and low economic costs, the proposed method of assessing frost resistance is many times superior to the method of direct freezing of plants
Results of three-years of examining the collection material of winter barley of different ecological-geographic origin are brought in this article. We have also allocated sources of high grain productivity, winter hardiness and precocity for further use in breeding programs