Name
Salfetnikov Anatoliy Alekseevich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
—
Articles count: 17
The problem of increasing resistance of winter
barley to adverse conditions of winter is the most
problem in the selection area of this crop. The main
component of this complex trait is the frost
resistance. Laboratory methods of assessment of
frost resistance are important for breeding of winter
barley. These methods can effectively replace more
costly method of direct freezing in the freezers.
Magnesium cations (Mg++) are essential
components of the protein-synthesizing system of
germinating seeds. Magnesium cations determine
the stability and translational activity of mRNA and
rRNA. Trilon B is usually used for in vitro banding
of magnesium cations in area of biochemistry of the
nucleic acids. It was very interesting to verify the
Trilon B influence to seed germination of winter
barley varieties by experiment. Experiments
showed: actinomycin D – the inhibitor of RNA
synthesis – didn’t show varieties specific effect to
seed growth of winter barley, while Trilon B had a
varieties specific impact to length coleoptiles and
roots. Research was carried out on etiolated
seedlings at temperatures from 22 to 28oC, and different concentrations of Trilon B - 1,6×10-3Ðœ,
2,4×10-3Ðœ and 3,2×10-3Ðœ. The roots of 3-days old
seedlings were more sensitive to Trilon B influence.
It is shown the varieties of Russian origin were
naturally reacted to Trilon B: the higher the frost
resistance of variety, the more resistant variety to
the action of various concentrations of Trilon B at
different temperatures. The most optimum
concentration was 3,2 × 10-3Ðœ at 22-25oC.
Varieties by foreign origin deviated from this
pattern. It is interesting to research the genetic
essence of these differences. It will be helpful for
optimization of varieties differentiation conditions
to create a laboratory method of estimate of frost
resistance of winter barley. The new method will be
most efficient compared the direct freezing method
The differential stability of mRNA is an important
mechanism for posttranscriptional regulation of gene
expression in eukaryotes. Messenger RNA stability is
controlled by specific genes and growth conditions.
The review examines the theoretical possibility of
mRNA stabilization in vivo as a consequence of
replacing carbon atoms (C) or phosphorus (P),
composed of nucleic acid on the silicon (Si) atom.
During isolation of poly-(A)+mRNA from plant
tissues by the two-cycle affinity chromatography on
poly-(U)-Sepharose, regular changes in poly-
(A)++mRNA yield were observed. The changes
varied both with the plant genotype and growth
conditions. Celite treatment of heated and unheated
total RNA preparations from developing corn kernels
and from green and etiolated wheat seedlings.
Messenger RNA that differed in the length of poly-
(A)-sequences was used for hybridization. It is
evident that a reduction of poly-(A)-length causes
alterations in spatial structure of mRNA, and
associated proteins and cations Mg++ become
accessible to celite absorption. Heating promotes
melting of secondary structure, already initiated, and
increases the efficiency of mRNA stabilization by
celite. Interpreting the facts interact celite with mRNA
in vitro and stabilization of mRNA in vivo by
cycloheximide with a modern point of view can be considered with the position research of the
phenomenon of RNA interference
The review examines research unusual properties of
RNA. RNA has the ability to act as both genes and
enzymes (ribozymes). This property could offer a
way around the «chicken-and-egg» problem: genes
require enzymes; enzymes require genes.
Furthermore, RNA can be transcribed into DNA, in
reverse of the normal process of transcription. These
facts are reasons to consider that the RNA world
could be the original pathway to cells. The general
notion of an «RNA World» is that, in the early
development of life on the Earth, genetic continuity
was assured by the replication of RNA and
genetically encoded proteins were not involved as
catalysts. There is now strong evidence indicating
that an RNA World did indeed exist before DNAand
protein-based life. RNA has multiple functions.
Among these, "messenger RNA" carries genetic
information from DNA to protein formation. RNA is
often a single-stranded spiral, but also exists in
double-stranded form. In 1998, Craig Mello and
Andrew Fire discovered through their studies of the
roundworm C. elegans a phenomenon dubbed "RNA
interference". In this phenomenon, double-stranded
RNA blocks messenger RNA so that certain genetic
information is not converted during protein
formation. This "silences" these genes, i.e. renders
them inactive. The phenomenon plays an important
regulatory role within a genome. Recent years have
been perhaps the most fruitful period yet in terms of
research in the area of mRNA stability (Phenomena:
Gene Silencing; RNA interference; Identity of
mRNA decay in vivo and in vitro). The elaboration
of new methods in biothechnology have been
presented
Due to the wide spread of winter barley varieties with
different Phytopathologic characteristics, a serious
threat to the crop is dwarf rust caused by a pathogenic
fungi of the Puccinia. The article presents the results of
phytosanitary studies conducted in different agroclimatic
zones of the North Caucasus region for the
period from 2013 to 2015. Electronic maps of levels of
development and distribution of dwarf rust are based on
phytosanitary inspection. On the experimental field
called «Kuban» as well as rust nursery of VNIIBZR we
have performed an immunological evaluation of the
sustainability of winter barley accessions to the
pathogen of the dwarf rust on natural and artificial
infectious backgrounds in the period from 2013 to
2015. The article reflects the objective characteristics of
varieties and lines of winter barley with a relative
stability to the North Caucasian population Puccinia
hordei. The most promising varieties and lines of winter
barley of the KubGAU breeding were selected during
operation. These varieties and lines are highly resistant
to rust and dwarf, they also have other valuable
features. The article presents information on the best
yield varieties and lines of winter barley as well as on
gross yield and sowing area
100 years ago, on June 4, 1920, 32-year-old Professor of the Saratov University Nikolai Ivanovich Vavilov (1887-1943) first reported at the III all-Russian selection Congress at the University of Saratov on his discovery of homological series in the study of parallelisms in the phenomena of hereditary variability by analogy with homological series of organic compounds. This discovery in genetics received the rank of law, the only one after the laws of G. Mendel. This major study was a further development of the genetic idea of C. Darwin on the origin of species. It showed the ways in which close species and genera of plants have a parallel formative process, because the crucial in the process of evolutionary development of living organisms – first of all, their genetic features. In cases where the development of a trait requires the joint and consistent action of many genes, the occurrence of homological series is inevitable, and this does not contradict the random variability of C. Darwin. In addition to its great genetic significance as a law of evolution, the law of homological series in hereditary variability is of great importance for botanists, plant breeders and breeders: it not only determines the place of each form in the plant world, but can also indicate to the breeder possible directions in his practical work. According to a number of geneticists and breeders, if G. Mendel discovered the rules of heredity, then N. I. Vavilov discovered the rules of variability