Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
AGRIS logo UlrichsWeb logo DOAJ logo

Name

Nenko Nataliya Ivanovna

Scholastic degree


Academic rank

Honorary rank

Organization, job position

State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
   

Web site url

Email


Articles count: 7

Sort by: Date Title Views
328 kb

CHANGING THE PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF THE IMMUNE RESPONSE OF GRAPE PLANTS OF DIFFERENT PHYTO-SANITARY CONDITION

abstract 1041410104 issue 104 pp. 1488 – 1498 30.12.2014 ru 939
The results of the study of the dynamics of physiological and biochemical parameters of the immune response of grapes to fungal pathogens are given in the article
258 kb

HYGROSCOPICITY OF MATURE GRAIN AS A MARKER OF WINTER BARLEY AND SUNFLOWER FROST RESISTANCE

abstract 1451901012 issue 145 pp. 7 – 30 31.01.2019 ru 97
In the review article we present a comparative analysis of frost resistance of varieties of winter barley and sunflower (Helianthus annuus L.) by the results of freezing in the refrigerating chambers and by the hygroscopicity of mature grain. On the example of a number of varieties of both cultures, it is shown that the higher the frost resistance of the variety, the smaller the volume of the supernatant can be obtained by extracting the press cake with a solution containing magnesium cations. It is assumed that increased hygroscopicity of winter barley and sunflower press cake is associated with a relatively high content of so - called "water-soluble starch" - β-glucan polysaccharides in its grain. The possibility of regulation of the degree of hygroscopicity under the influence of potassium (KNO3), zinc (ZnSO4) and gibberellic acid (GA3) was studied on a number of sunflower varieties. It was shown that treatment with a solution of potassium (a concentration of 50 ppm KNO3) of sunflower plants at the stage of 4 leaves leads to a significant increase in hygroscopicity of grain and hardiness of plants, little effect was given treatment with zinc (30 ppm ZnSO4) and a lesser effect was observed when treating plants gibberellovaja acid (20 ppm). Potassium treatment significantly intensified the biosynthesis of free Proline, phenolic compounds and soluble proteins. When processing zinc significantly increased the content of carbohydrates in sunflower plants. Hygroscopicity showed a significant correlation with the content of free Proline (R2=0.621), phenolic compounds (R2=0.907), total carbohydrates (R2=0.673) and soluble proteins (R2=0.708). It is assumed that the treatment of plants with potassium and zinc plays a key role in increasing the hygroscopicity of grain. The results of comparative studies of frost resistance on the degree of survival of plants during freezing in cold rooms and the degree of hygroscopicity of mature grain showed that these two methods of assessing frost resistance give very close data. At the same time, in terms of simplicity and low economic costs, the proposed method of assessing frost resistance is many times superior to the method of direct freezing of plants
288 kb

MOLECULAR MARKERS OF THE EFFECT OF INTERACTION "GENOTYPE-ENVIRONMENT" IN PLANTS ON THE BASIS OF THE DECAY OF mRNA IN VIVO (RNAi) AND IN VITRO (OMMP-SYSTEM)

abstract 1411807012 issue 141 pp. 41 – 64 28.09.2018 ru 69
This overview and theoretical article deals with the consideration of hypothetical possibilities for the development of molecular-kinetic markers of agricultural plants allowing to quantify the effect of genotype-environment interaction on the basis of stability studies of mRNA. The development in view is based on the results of studies of the mRNA decay identity in vivo and in vitro (the ommp system), as well as the phenomenon of RNA interference (RNAi), widely studied in plants. The ommp system has allowed to establish the relationship of the cultivar-specific growth reaction to the effects of low positive temperatures, dehydration, salinity, illumination and biologically active substances with the stability of the total and a number of gene-specific mRNAs of green and etiolated seedlings of winter soft wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.). Similar studies of mRNA stability have been conducted on ripening grains of maize (Zea mays L.), with a particular result of the in vivo and in vitro decay identity confirmed for major mRNAs of stored proteins, 19 and 22 kDa zeins, of normal maize and mutant according to the regulatory gene opaque-2, changing the amount and stability of zein mRNA in the maturing grain of high-lysine maize. Regulatory response of the organism through RNAi is also multiple and includes neutralization of viral and bacterial infections, reaction to pathogens and biologically active substances, circadian rhythms, water stress, hypoxia, mechanical stress, mineral nutrition, salt stress and temperature changes. Unfavorable environmental influences result in an increase or decrease in the expression of certain microRNAs (miRNAs). The change in mRNA stability is an important component of the gene expression regulation system in eukaryotic cells. The main determinants of mRNA stability are in the 3'-untranslated region. It is the It is the (U)nА sequence and the degree of polyadenylation of mRNA, i.e. length of its terminal homonucleotide chain. It is to this region that mRNA molecules are complementary to miRNA. The most important component, which largely determines the regularity of the genotype-environment interaction, is the polyadenyl sequence at the 3 'end of the mRNA. Its length depends both on the genotype and on the environmental conditions. There is evidence that polyadenylation degree of mRNA determines the secondary structure of the molecule. As is known, deadenylation of mRNA reduces its lifetime, and when the poly-A-tail reaches several dozens of nucleotides, an explosive disintegration of the mRNA molecule occurs. Consequently, in a living cell the following mRNA decomposition scheme appears to be logical: shortening the poly-A-tail of the mRNA opens the sites of miRNA interaction with the 3'-non-coding region of the mRNA molecule, which causes its degradation. Thus, it is safe to assume that the interaction of mRNA and miRNA in the ommp system is the underlying process for molecular-kinetic markers under development
322 kb

STABILIZATION OF MRNA CEREALS IN VITRO UNDER THE SILICON INFLUENCE

abstract 1321708056 issue 132 pp. 685 – 719 31.10.2017 ru 187
The differential stability of mRNA is an important mechanism for posttranscriptional regulation of gene expression in eukaryotes. Messenger RNA stability is controlled by specific genes and growth conditions. The review examines the theoretical possibility of mRNA stabilization in vivo as a consequence of replacing carbon atoms (C) or phosphorus (P), composed of nucleic acid on the silicon (Si) atom. During isolation of poly-(A)+mRNA from plant tissues by the two-cycle affinity chromatography on poly-(U)-Sepharose, regular changes in poly- (A)++mRNA yield were observed. The changes varied both with the plant genotype and growth conditions. Celite treatment of heated and unheated total RNA preparations from developing corn kernels and from green and etiolated wheat seedlings. Messenger RNA that differed in the length of poly- (A)-sequences was used for hybridization. It is evident that a reduction of poly-(A)-length causes alterations in spatial structure of mRNA, and associated proteins and cations Mg++ become accessible to celite absorption. Heating promotes melting of secondary structure, already initiated, and increases the efficiency of mRNA stabilization by celite. Interpreting the facts interact celite with mRNA in vitro and stabilization of mRNA in vivo by cycloheximide with a modern point of view can be considered with the position research of the phenomenon of RNA interference
293 kb

THE APPLICATION OF FUROLAN PLANT GROWTH REGULATOR AND IMMUNIZER ON SUNFLOWER CULTIVATION IN THE KRASNODAR REGION

abstract 1211607093 issue 121 pp. 1522 – 1544 30.09.2016 ru 305
Sunflower is one of the most important oilseed crops, which are of great economic importance in Russia and in the world. It is very adaptive crop in terms of climatic conditions range, for that reason it is cultivated on a vast territory of the Russian Federation in various weather conditions. Sunflower oil has high nutritional and taste qualities, it is used numerously in food and is applied in various fields of food industry. The biologically active linoleic acid, phosphatides and fat-soluble vitamins A, D, E and K, which are of great nutritional value to humans, are contained in the oil. According to its calorific capacity, sunflower oil is on the first place among vegetable oils. Due to biological characteristics of sunflower, the Krasnodar region is the most favorable region of the Russian Federation for obtaining high and stable yields of this crop. However, here the drought is observed during the summer period and it affects adversely the productivity and quality of sunflower seeds. The increasing of resistance to unfavorable weather conditions is possible only based on detailed study of physiological features of productivity formation and seeds quality that is highly important task in view of the current geopolitical situation in Russia. One way of solving this issue is the appliance of the growth regulators, possessing anti-stress activity that improve the quality of sowing seeds and increase the productivity and plant resistance to stressful environmental factors. These drugs include growth regulator called Furolan, which was created in KubGTU and is certified for use in Russia. It is not toxic and is used in nano-dozes, there is no its residual quantities in the products and environment. Furolan has a positive effect on physiological and biochemical processes, improves the productivity of plants, their resistance to unfavorable growing conditions by increasing the resistance to dehydration as well as to the risk of fungal diseases
191 kb

THE ECOLOGICAL AND ECONOMICAL EVALUATION OF FUROLAN TREATMENT IN SUNFLOWER CULTIVATION IN THE KRASNODAR REGION

abstract 1211607092 issue 121 pp. 1504 – 1521 30.09.2016 ru 469
Sunflower is one of the most important oilseed crops, which are of great economic importance in Russia and in the world. It is very adaptive crop in terms of climatic conditions range, for that reason it is cultivated on a vast territory of the Russian Federation in various weather conditions. Sunflower oil has high nutritional and taste qualities, it is used numerously in food and is applied in various fields of food industry. The biologically active linoleic acid, phosphatides and fat-soluble vitamins A, D, E and K, which are of great nutritional value to humans, are contained in the oil. According to its calorific capacity, sunflower oil is on the first place among vegetable oils. Due to biological characteristics of sunflower, the Krasnodar region is the most favorable region of the Russian Federation for obtaining high and stable yields of this crop. However, here the drought is observed during the summer period and it affects adversely the productivity and quality of sunflower seeds. The increasing of resistance to unfavorable weather conditions is possible only based on detailed study of physiological features of productivity formation and seeds quality that is highly important task in view of the current geopolitical situation in Russia. One way of solving this issue is the appliance of the growth regulators, possessing anti-stress activity that improve the quality of sowing seeds and increase the productivity and plant resistance to stressful environmental factors. These drugs include growth regulator called Furolan, which was created in KubGTU and is certified for use in Russia. It is not toxic and is used in nano-dozes, there is no its residual quantities in the products and environment. Furolan has a positive effect on physiological and biochemical processes, improves the productivity of plants, their resistance to unfavorable growing conditions by increasing the resistance to dehydration as well as to the risk of fungal diseases
142 kb

THE INFLUENCE OF THE PLUM POX POTYVIRUS (PPV) ON THE CONTENT OF PIGMENTS, PROTEIN, LIGNIN, WATER IN TISSUES OF LEAVES OF PRUNUS DOMESTICA

abstract 1271703029 issue 127 pp. 432 – 441 31.03.2017 ru 146
The results of the study of physiological and biochemical parameters of plum plants, changing when infecting plants Plum pox potyvirus (PPV) are shown. It was found that the infected leaves at the stage of full development of the content chlorophyll a reduced by 30 % in variety Stanley and by 6 % in Kabardinskaya rannaya, chlorophyll b in less than 49 % at Stanley, 37% in Kabardinskaya rannaya, carotenoids are less than 22 % in the variety Stanley and by 11% in the variety Kabardinskaya rannaya . Leaves infected with the virus pox (PPV), in comparison with not infected, the amount of protein were decreasing: in the variety Stanley by 21 %, in the variety Kabardinskaya rannaya by 28 %. In the period of active growth of the leaves change in the content of pigments and protein is more intense. The content of lignin in the tissues of leaves, infected with the virus pox (PPV), in comparison with not infected in the variety Stanley was below on 13 % and in the variety Kabardinskaya rannaya - 8 %. The total content of water of infected tissue on the stage of a full-time development is reduced in patients leaves as compared with not infected 11 % in the variety Stanley and by 1 % in the variety of Kabardinskaya rannaya. Throughout the period of development of foliage studied parameters vary depending on the variety
.