Name
Vasylyk Irina Aleksandrovna
Scholastic degree
•
Academic rank
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Honorary rank
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Organization, job position
Institut of Vine and Wine “Magarach”
Web site url
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Articles count: 4
Peculiarities of breeding oidium-resistant genotypes in
crossing Crimea autochthonous grape varieties with
complex interspecific hybrids were studied. Twentytwo
populations consisting of 1323 seedlings were
analyzed over a period of 2008-2014. The highest
oidium resistance was registered in the progeny of the
cross Khersonesskii х ЖС 26-205 (6.8 points), and the
average estimate of oidium resistance across all the
study populations was higher than that of the initial
autochthonous grapes of the Crimea. The level of
hypothetical heterosis in the majority of the study
populations indicates that, on the whole, the hybrid
seedlings possess a higher oidium resistance relative to
the initial Crimea autochthonous grapes. The highest
breeding value was associated with cross combinations
having the complex interspecific hybrids Muscat JIM,
Spartanets Magaracha and Magarach 31-77-10 in their
pedigrees. The cross combination Muscat JIM х
Shabash was the most efficient, yielding 10.7 per cent
of oidium-resistant seedlings. It was reliably
established that a high level of the genotypically determined inheritance of oidium resistance is found
in crosses with the participation of the interspecific
forms Magarach № 31-77-10 and Muscat JIM (female
parents) and Spartanets Magaracha and Tsitronnyi
Magaracha (male parents). The expediency of this
direction of breeding research was proved
Progress of breeding for resistance to diseases, pests
and low temperatures is provided by cross-species
hybridization. Institute of Vine and Wine "Magarach"
is one of the leading centers of selection of grapes in
the world. The breeding program of deducing grapes at
the institute is based on the study and attracting the
world's gene pool, including and industrial of the
Russian Federation assortment of grapes. Analysis
assortment shows that the share of productive, highquality
varieties with group resistance to diseases,
pests and extreme environmental factors is very
small. The main method of selection of grapes of a
new generation who have genetically determined traits
earliness, resistance to biotic and abiotic factors is
crossing complex interspecific hybrids and forms of
their involvement with each other and with the forms
of species Vitis vinifera, belonging to different ecogeographical
groups, saturating crossing between
hybrid forms with complex features, the appropriate
selection of reference. For vigorous offspring is most
effective as a parent to use a local variety of forms of
Crohn and father - interspecific cultivar Alminsky
The article highlights the issues of grape plants resistance
to low temperatures; it is an urgent problem for
all wine-growing regions of the world, located in the
zone of risky agriculture. Losses caused by frost, indicate
long-standing necessity of perfection of assortment
in terms of enhancing its hardiness by enriching
more resistant genotypes obtained both through the
introduction or naturalization, as well as from the
crossing of indigenous with interspecies hybrids. Methodology for evaluating frost resistance properties
of the material served as a preparation for the originality
of his research and experimental evaluation of the
unique divisibility properties of the aim. Thus, we initially
conducted hardening of matured cuttings at positive
temperature plus 8 plus 4 ° C for 14 days (I
quenching phase) and a negative temperature minus 5 °
C-minus 7 ° C for 9 days and minus 10 ° C-1 day (II
hardening phase). Then the cuttings we frozen in the
temperature range from minus 16 ° C to the next steps
of consistent freezing (minus 16 ° C - 2 days; minus 18
° C - 2 days; minus 20 ° C - 2 days; minus 22 ° C –
1day, minus 24 ° C - 1 day, minus 26 ° C - 1 day, minus
28 ° C - 1 day and minus 30 ° C - 8 hours). After
freezing of cuttings of each variety, the sample was
placed on the 3 days in the refrigerator at the temperature
of + 2 ° C to their gradual thawing. Cuttings were
grown at room temperature on water. As a result, obtained
by the accounting: the lowest frost-resistance
among the studied forms of parental Crimean origins
was found in grades of Shabash, Soldayya and Solnechnodolinsky;
the varieties of Dzevat kara, Kokur
bely, Kokur cherny, Misket, Misgyuli kara, Sari Pandas
were set as a weak level (-20 °C); Aybatly, Kefesiya,
Kok pandas and Ekim kara have average level up
to -22 °C; the highest frost-resistance - 24 °C – was
shown by Khersonessky and Kapselsky. Also, we have
identified synthetic frost-resistant F1-population and
plus-transgressive selection form
Черешки листьев, взятых из растений ин витро, девяти межвидовых гибридов винограда: подвоев Берландиери х Рипариа ‘Кобер 5ББ’, Рипариа х Рупестрис ‘101-14’ и сортов (cvs): 'Бианка', 'Зигфридребе', 'Подарок Магарача’, ‘Первенец Магарача’, ‘Цитронный Магарача’, ‘Интервитис Магарача’ и гибридной формы ‘Магарач 100-74-1-5' культивировали на твердой среде NN, содержащей различные концентрации 2,4-D и BА в различных сочетаниях. Для того, чтобы инициировать рост клеточных суспензий, проэмбриогенные каллусы пересаживали в жидкую среду NN, дополненную 1.0 мг/л 2,4-Д и 0,2 мг/л BА. Субкультивирование этих суспензий в жидкую среду NN, дополненную 2 мг/л NAA и 0,1 мг/л BА, привело к развитию агрегатов эмбриоидов, а пересадка в жидкую среду с 0,5 мг/л BА вызвала развитие единичных глобулярных и сердцевидных эмбриоидов. Проэмбриогенные клеточные суспензии, состоящие из преимущественно отдельных клеток, могут быть использованы в трансформации генов и селекции на клеточном уровне с целью уменьшения вероятности появления химерных растений