Name
Besedina Ekaterina Nikolaevna
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
Web site url
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Articles count: 1
The stage of introduction of plantlets to in vitro
culture is an important stage of technology of
clonal micropropagation of plants. For the
purpose of decrease in a share of the plantlets that
were lost from an infection and increase of their
regeneration ability, sterilizers and antibiotics,
effective and safe for apple rootstocks’ plantlets
wee allocated, and also favorable terms of
introduction to in vitro culture were allocated. As
a result of the conducted researches, we have
established that on influence on an nutrient
medium and plantlets infection and on growth and
development of plants, beneficial effect on apple
rootstocks’ plantlets has an antibiotic nystatin of
200 mg/l: the reproduction coefficient on the
medium with this antibiotic is equal 4,3, in the
same concentration nystatin has the sanifying
effect of 60-75% for stocks of SK 2 and MM 106.
During researches of sterilizers for apple
rootstocks’ plantlets SK 2, SK 3, SK 4, SK 7,
MM 106 as an alternative to widely applied
highly toxic sterilizer corrosive sublimate (the
first class of danger) were picked up effective and
safe preparations for sanitation of plantlets from
an infection, such as the household preparation
"Whiteness" (sodium hypochlorite) in cultivation
1:2, low-dangerous substance of the fourth class
of danger (a share of viable plantlets of 75,5%
from initially introduced), and also fosfopag, a
preparation of the fourth class of danger (a share
of viable plantlets of 65% from initially
introduced). The favorable term for a meristem of
apple rootstocks’ plantlets’ introduction to in vitro
culture are phases of buds’ burgeoning (March)
and the intensive growth of shoots (May – June)