Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Raduk Anastasia Vladimirovna

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Don state agrarian University
   

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Articles count: 1

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OPTIMIZATION TECHNIQUES FOR PCRRFLP FOR GENOTYPING SHEEP

abstract 1131509102 issue 113 pp. 1474 – 1482 30.11.2015 ru 700
Diagnosis by PCR-RFLP (polymerase chain reaction – polymorphism of the lengths of restriction fragments) is the standard analysis of point mutations for the diagnosis of allelic polymorphism of candidate genes related with productive qualities of farm animals. Along the length of the fragments (RFLP) make a conclusion about the absence or presence of the point mutation, and homozygosity or heterozygosity of the individual. The aim of our work was the optimization of protocols for conducting PCR-RFLP analysis for genotyping sheep for genes of the growth hormone gene differential growth factor and gene of calpastatin
.