Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Gudkov Georgy Vladimirovich

Scholastic degree


Academic rank

professor

Honorary rank

Organization, job position

Kuban State Medical University
   

Web site url

Email


Articles count: 2

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299 kb

NON-INVASIVE DIAGNOSTICS OF ANEUPLOIDY OF A FETUS WITH CIRCULATING TROPHOBLAST CELLS IN MATERNAL BLOOD

abstract 1231609131 issue 123 pp. 1965 – 1984 30.11.2016 ru 244
Isolation of fetal cells from the blood of a pregnant woman is more relevant for receiving the information of the genome of a fetus and implementation in clinical practice, non-invasive screening methods for prenatal diagnosis. The work proposes efficient ways of sorting trophoblasts circulating in maternal blood for the diagnosis of fetal aneuploidy in early gestation by analyzing microsatellite marker of the chromosomes that most prone to numerical aberrations (13, 18, 21, X and Y). A set of microsatellite markers allowed to reveal aneuploidy in three pregnant women, and also to exclude mosaicism and contamination of samples with maternal cells. A retrospective analysis of data on invasive cytogenetic studies (chorionic villus sampling) confirmed the results of the study
344 kb

SELECTION OF FETAL CELLS CIRCULATING IN MATERNAL BLOOD FOR NONINVASIVE PRENATAL DIAGNOSIS

abstract 1151601082 issue 115 pp. 1288 – 1309 27.01.2016 ru 177
Trophoblast cells circulating in maternal blood may serve as potential sources of genetic information for screening in obstetric practice approaches to noninvasive prenatal diagnosis. The material for the study was whole blood of pregnant women of gestational ages of 8-12 weeks in a volume of 10 ml. stabilized with heparin. Enrichment was performed by the method of gradient centrifugation and magnetic separation (CD45–) with fluorescent staining negative fractions labeled with antibodies to antigens of trophoblasts (HLA-G, and Trop-2) to conduct flow cytometry and sorting of cells on glass slides. Single cell candidates using laser micro dissection were transferred into a vial for subsequent whole-genome amplification, providing sufficient representativeness of their genome. Fetal origin of the genetic material was confirmed by comparison of alleles of the HLA genes of the parents and cells candidates. It is possible to improve significantly the accuracy and versatility of non-invasive prenatal diagnosis using comparative genomic hybridization using chips (аCGH)
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