Scientific Journal of KubSAU

The article gives the results of the study of an effect of mechanochemical powder of wood green of Abies (MPA) on potato morphogenesis in vitro. The study involved the hormone-free nutrient mediums (MS, ½ B5, ½ LS) for growing microcuttings from preliminary obtained aseptic plants. Plant morphogenesis was observed on the 15th and 30th days. At that, the number of developed shoots, plant height, leaves number, rhizogenesis rates, root number and length were estimated. MPA appeared to have the regulatory effect on morphogenesis of potato microcuttings. Based on mineral composition of the nutrient agar medium for potato microcuttings growing, MPA at 100 mg/l concentration induced root development (½ B5 medium), reduced height of shoots (MS medium), while the number of leaf blades was not affected

Along with traditional ways, the biotechnological methods become more significant in contribution to plant conservation in situ. The work is dedicated to elaboration and advancement of the method of isolated tissues and organs culture for conservation and reproduction of the badan, Bergenia crassifolia L., the rare species of Saxifrgaceae family. The conducted study yielded the regenerated plants of B. crassifolia from seeds. For the first time, we have performed conservation and reproduction of B. crassifolia in vitro under conditions of Yakutia