Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Radchenko Vitaly Vladislavovich

Scholastic degree


Academic rank

Honorary rank

Organization, job position

Kuban State Agrarian University
   

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vitalyradchenko@yandex.ru


Articles count: 2

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DEVELOPMENT OF OPTIMAL WAY OF OBTAINING A HYDROLYZATE OF LACTIC ACID BACTERIA

abstract 1321708121 issue 132 pp. 1465 – 1477 31.10.2017 ru 103
The work was carried out in the scientific-research laboratory of the chair of Biotechnology, Biochemistry and Biophysics of Kuban SAU, the aim of which is the search of optimal conditions of obtaining the hydrolyzates of lactic acid bacteria by thermoacid and enzymatic ways. The pure cultures of microflora of gastrointestinal tract of quails − Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius were the object of researches. Under conducting the thermal acid hydrolysis there were used the same correlations of bacterial mass and water as well as procedures of clearing of hydrolyzates. The variable conditions were рН, temperature and time of hydrolysis. When carrying out the enzymatic hydrolysis in this study there was used the enzyme from the group of muramidase lysozyme. The amount of enzymes and time of carried out hydrolysis were the variable conditions under obtaining the enzymatic hydrolyzates. In the process of researches there were studied: qualitative content of total protein, peptides with М.м. < 1500, protein and peptides with M.m. > 1500, aminoacids and as well as GMDP (glucosaminilmuramilpentapeptide). On the basis of carried out experiments there was stated that according to all components of biologically active substances the thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates by different ways was revealed at destruction of cells Lb. intermedius. Content of GMDP in thermal acid hydrolyzate was higher than in enzymatic on 0,028 g/100 ml (51,8 %)
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INTENSIFICATION OF THE CULTIVATION PROCESS OF PHYSIOLOGICALLY-ADAPTED LACTOBACILLI AS A BASIS FOR THE CREATION OF BIOLOGICAL PRODUCTS OF MICROBIAL ORIGIN FOR POULTRY BREEDING

abstract 1281704076 issue 128 pp. 1101 – 1114 28.04.2017 ru 193
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
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