Name
Usatov Alexander Vyacheslavovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
South Federal University
Web site url
—
Articles count: 2
In the Southern Federal University on the genetic basis
of sunflower inbred line 3629, a collection of plastid
mutants with varying degrees of chlorophyll
deficiency was created by inducing Nnitrosomethylurea.
Chlorophyll content was associated
with their photosynthetic activity. It was found that the
lower the chlorophylls content in mutant plastids, the
lower the sugar concentration in plant tissues. For
example, during the entire period of plant growth for
whites (1.0-3.0 % chlorophylls a+b from control) var-
10, var-17 and yellows (6.0-9.5 % chlorophylls a+b
from control) var-29, var-33 leaf areas of variegated
mutants depending on the development phase and the
content of green pigments are characterized by a low
(2-7 fold) sugar content. Yellow-green (75.5%
chlorophylls a + b from control) leaves of en:chlorina-
7 contain a higher level of carbohydrates, although it is
1.5-2 fold lower than at line 3629. Monosaccharides
are products of hydrolytic reactions catalyzed by β-
glycosidases. It was shown, that the activity level of β-
galactosidase and β-glucosidase exceeded the
corresponding indicators of the control at 1.5-2 and 2-7
fold, respectively. Similarly to enzymes from the
water-soluble fraction, membrane-bound β-
glycosidases also showed increased activity in the
leaves of the investigated mutants, compared to the
control green plants of 3629. Consequently, the
activity of β-glycosidases increases dramatically in
leaf tissues with deficiency of photosynthetic. Thus,
chlorophyll mutations can lead to a change in the
expression of nuclear genes, resulting in a significant
increase in the activity of β-glycosidases in the mutant organelles themselves
The results of laboratory experiments on the effect of different concentrations of SkQ3 (10- (6'- methylplastoquinonyl) decyltriphenylphosphonium) on the rate of growth of spring barley seedlings of varieties Sokol and winter wheat varieties Kalym in normal and under inadequate moisture conditions, as well as data from field experiments on the effect of pre-sowing processing SkQ3 on the morphometric indices of spring barley varieties Shhedry, hard winter wheat of the Lazurite variety and soft winter wheat of the Lydia variety are presented. As a result of laboratory experiments, an increase in the growth rate of 14-day-old seedlings grown in solutions of polyethylene glycol was shown when treating the seeds with 2.5 nM and 25 nM with SkQ3 solutions.
In the field experiments on the pre-sowing treatment of SkQ3 seeds, an increase in the morphometric indices of spring barley varieties of generic Lydia soft winter wheat and hard Lazurite winter wheat has been established, as well as an increase in the yield of winter wheat of both varieties as compared to the control