Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Suzina Nataliya Egorovna

Scholastic degree


Academic rank

Honorary rank

Organization, job position

Institute of Biochemistry and Physiology of Microorganisms
   

Web site url

Email

suzina_nataliya@rambler.ru


Articles count: 2

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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY

abstract 1341710034 issue 134 pp. 404 – 426 29.12.2017 ru 502
A method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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MORPHOLOGICAL FEATURES OF I-FORMS OF PF-RELATED BACTERIOPHAGES IN PSEUDOMONAS AERUGINOSA PAOI LYZATES BY EXTRACTS OF EUROPEAN BIZON’S FEKALIA

abstract 1491905036 issue 149 pp. 0 – 0 31.05.2019 ru 18
In this work, we have found shortened particle forms of the filamentous bacteriophage Pf, when the lysis of the Pseudomonas aeruginosa PAO1 strain was induced by extracts of bison feces to study the intestinal bacteriophages of this animal. The sizes of the shortened particles from 250 to 950 nm and their thickness 17 nm were determined. The particles had a slightly pointed one end and sometimes a flag on the other extended end. The particles were formed in the process lysis of this strain by other bacteriophages. These lysates also contained a high concentration of vesicles from the pseudomonad membrane. The article discusses the mechanisms of prophage induction in the Pseudomonas aeruginosa PAO1 strain due to phage-phage induction and substances of the investigated extract
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