Scientific Journal of KubSAU

In cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time

We have described the theoretical assumptions and methodological solutions in the present review. They are innovative possibilities in the development of laboratory methods for assessing biological features of grain during the selection process

The main aim of this article is to generalize the results of biochemical and molecular research of developing opaque-2 maize seeds (high-lysine mutant of maize) metabolism peculiarities

This review covers the advances of the molecular biology in the study of gene expression characteristics of animals in the amino acid imbalance

This review is devoted to the 60 anniversary of the winter wheat variety (Triticum aestivum L.) Bezostaya 1 – a masterpiece of world selection. This variety played a great role in increasing total grain yields in the Kuban Region. It also was an important gene source for Russian and world breeding. This variety has initiated and was for many years a party to research molecular biological mechanisms of wheat frost resistance formation. The article summarizes data about the peculiarities of functioning of protein-synthesizing system it sprouts (coleoptilies) under the influence of hardening temperature (4oC): translational activity in vitro, poliribosomts, degree of polyadenilation and stability of total mRNA and specific mRNA translational elongation factor α subunit 1 (eEF-1 α). The in vitro (ommp) system, which adequately characterizes the relative mRNA stability in vivo was used for these measurements. The effects of cooling and light on mRNA stability were shown to be cultivar-specific. The hardiness of winter wheat has a positive relationship with the amount of RNA and DNA, and a negative correlation with the amount of magnesium cations in mature grain that can serve as a simple marker of frost resistance. The main feature of the variety Bezostaya 1 is high content of magnesium ions (Mg++) in RNA molecules and relatively low variation of Mg++-depending molecularphysiological responses for different environmental conditions. The knowledge gained in the study of variety of Bezostaya 1 contributes to the understanding of molecular biological processes that underlie the selection and determining its future success