Scientific Journal of KubSAU

In the article, we have studied the influence of biological nitrogen deposition on the physical and chemical parameters of natural semi-sweet wine, on the example of two grape varieties – classic European Chardonnay and interspecific hybrid Ekaterinodar, including amino acids and aroma-forming components. It is established, that the use of the proposed technology contributes to the active consumption of amino acids by yeast in the fermentation stage. At the same time, yeast cells consumed most actively alanine, valine, phenylalanine, histidine, aspartic, aminobutyric, glutamic acids, leucine, lysine, Sirin, tyrosine, threonine. A slight increase in the concentration of amino acids was observed at the end of fermentation, when the yeast cells entered the stationary phase of development. However, the content of all amino acids, with the exception of Proline, in the wine material was less than in grapes and fermenting wort. As a result of biological nitrogen deposition in experimental versions, the concentration of nitrogen compounds (total and amine nitrogen) was three times less than in the control ones. There is a difference in the concentration of flavor-forming components in wines prepared from both grape varieties using different technologies. In experimental versions, we observed a greater accumulation of almost all components of the aroma-binding complex (especially esters, terpenes, phenylethanol, and ionone), with the exception of higher alcohols. Experimental samples of natural semi-sweet wines from the Chardonnay and Ekaterinodar grape varieties were characterized by a bright floral and fruit aroma and had a higher tasting rating in comparison with the classic technology

We have presented opportunities to improve the nutritional value of wine production: improvement of biogenetic soil, using organic fertilizers. The nutritional value is ensured by not only high-quality biochemical diversity of berries, but also the safety of products that do not contain toxic inclusions. Bio-fertilizer stimulates the biotransformation of toxic soil residues to replenish soil nutrients for the plant, providing grape raw material with biologically active substances and improves the nutritional value of wine products. Food safety of grapes is ensured by reducing it residues of hazardous chemicals migrating from the soil into the plant and the grapes. It has been established, that after 3 years of application of biofertilizers we significantly improved food safety and quality in the biochemical parameters, especially important for the classic wine grape varieties. The soil content of toxic residues in grape raw material is reduced to 50%, the amount of organic acids increases by: 4,22 mg/dm3(Gallic, coffee acid, chlorogenic; 0.7 g/cm3 amber; of 1.33 mg/dm3 ascorbic; 0.25 mg/dm3 nicotinic

The article is dedicated to the study of new complex growth regulator called Zerebra agro and its influence on agrobiological and technological indicators of grapes. Studies were conducted in the Anapo-Taman Wine Growing Zone of the Krasnodar region (PAO Pobeda, Temryuk District). The fruit-bearing plantations of the white variety Viorica were cultivated three times during the vegetation period: before flowering (May 27), after flowering (June 20), the growth phase of berries (July 18), berries ripening (August 13) 200 ml / ha. Application of the growth regulator Zerebra agro resulted in a significant increase in the average mass of the bunch (by 11.5%), due to an increase in the number of berries (by 12.6%), yields from the bush (by 11.8%) and yields of plantations (by 1.17 tons or %). The increase in the experimental variant of the vine harvest was not accompanied by a decrease in the sugar content of the berries juice and an increase in the titrated acidity. The use of Zerebra agro allowed to reduce the degree of death of the central buds of wintering eyes at two and a half times, also to increase the coefficients of fruiting and fruit bearing, as well as the proportion of eyelets with two inflorescences and more. The greatest decrease in the death of the central wintering buds, as well as an increase in the embryonic fruiting index, was observed in the zone of 1-5 buds, which makes it possible to apply in the experimental version a short pruning length of fruit vines and to abandon the dry garters of fruit shooters

The article presents the results of the fertilizers use effect on dry wine materials produced from the Chardonnay variety, grown in the Anapo-Taman soil and climatic zone of the Krasnodar region, Russia. As a result of wine materials analysis, increase of tartaric acid was noted, with the autumn application of phosphorus-potassium fertilizers and the spring application of ammonium nitrate. At the same time, its optimum content was noted with nitroammophos. Also, the minimum concentration of malic acids was detected by the application of nitroammophos with potassium fertilizers. In its turn, early spring application of ammonium nitrate reduced the concentration of malic acid below the control sample (without fertilizers). It was revealed that the use of mineral fertilizers in this winemaking zone also contributed to a reduction of lactic acid in the must. At the same time, the application of nitroammophos (especially together with other mineral fertilizers), contributed to the accumulation of citric and succinic acids, as well as phenolic compounds. Moreover, the application of phosphate-potassium fertilizers in the autumn contributed to the accumulation, and, consequently, also to the increase in the content of phenolic compounds in the resulting must. In addition, the studied samples of wine materials contained high alcohol concentrations and low concentrations of sugars with volatile acids. At the same time, the maximum amount of alcohol exceeding its content in the control sample was revealed when phosphorus-potassium fertilizers were used together with nitroammophos fertilizers. It is also noted that the application of ammonium nitrate did not affect this indicator. As a result, the use of mineral fertilizers in the studied concentrations made it possible to prepare wine materials that contained a low concentration of sulfur dioxide, which is substantially lower than the harmful concentration for humans. In addition, increased concentrations of hydrogen ions in variants with the application of phosphate-potassium fertilizers have made it possible to reduce the number of iron tanates and the intensity of oxidation-reduction processes

The article reviews the results of studying the influence of mineral fertilizers on the qualitative indices of the Merlot grape variety in the Anapo-Taman zone of the Krasnodar region. The technology of cultivation of grapes on the experimental plot was in accordance with the accepted for the given zone and culture. Agrobiological work was carried out at the optimal time and had a high quality of execution. Grape bushes are laid in a 3.0 x 2.0 m pattern. Formation - a twoshoulder horizontal cordon. On the bushes we formed the same load of shoots and bunches. Scheme of the experiment: option 1 – no fertilizers (control); option 2-superphosphate with a mixture of potassium salt (P90K90) from the autumn; option 3 –nitroammofoska (N120P120K120) from autumn; option 4 – ammonium nitrate (N60) in early spring. Analysis of the results of studies on the study of biological features of growth, fruiting, yield and quality of Merlot grape varieties on the background of the use of mineral fertilizers shows their high efficiency in the conditions of the AnapoTamanskaya zone of the Krasnodar region and can be recommended for use in production conditions. Mineral fertilizers ensures the receipt of dry bulk wine of superior quality control sample (without fertilizers) the concentration of tartaric acid 17.3 25.1 percent, phenolic compounds 17.1 – 40.0 %, and alcohol content of 7.2 and 9.3 %, titratable acids 11.8 and 13.7 %, given extract by 17.4 %, with a decrease of the mass concentration of total sulfur dioxide and pH. The most effective is the introduction of NPK (N120P120K120)

Researches on non-root additional fertilizing of grapes with complex water-soluble fertilizer called Nutrivant plus have been conducted in corporation "Pobeda" in Temrjuksky area of Krasnodar territory on the implanted plantings of technical grade Rhine Riesling. In the test, the following kinds of Nutrivant plus were used: oleiferous (N0P20K33), sugar beet (N0P36K24), grapes (N0P40K25), universal (N19P19K19), grain (N6P23K35). Each mark of Nutrivant plus contained a certain set of microelements. The test consisted of six variants:1 variant - without additional fertilizing(control); 2 variant: grapes - before flowering, grapes - a growth phase of berries (a berry about a pea), oleiferous - the beginning of maturing of berries; 3 variant: universal - before flowering, universal - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 4 variant: grain - before flowering, grain - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 5 variant: Sugar beet - before flowering, a sugar beet - a growth phase of berries (a berry about a pea), a sugar beet - the beginning of maturing of berries; 6 variant: universal - before flowering, grain - a growth phase of berries (a berry about a pea), grapes - the beginning of maturing of berries. Norm of the expense of fertilizer is 3 kg/hectares. The expense of a working liquid - 700 l/hectares. The result was, that in the third, fourth and fifth variant of the test the increase in a crop from a bush happened accordingly on 7,5, 13,8 and 17,8 %. The maximum increase of a crop in the fifth variant was accompanied by increase in mass concentration of sugars in juice of berries on 0,6 g/100 см3и decrease titratable acidities for 0,6/dm3 As a result triple not root additional fertilizing of grapes plants grade Riesling with Nutrivant plus (universal - before flowering, grain - in a growth phase of berries and grape - in the beginning of maturing of berries) promoted more optimization of a water mode of leaves, increase in weight of a cluster, a crop from a bush and productivity, mass concentration of sugars and phenolic substances in juice of berries

Hereby we present the results of the research devoted to study of influence of growth modulator called melaphen on the technical brand of gapes such as Sovinion White and its agro-biological characteristics. The research was conducted in 2014-2015 using fruitful grape Sovinion White of 2004 planting as a subject in agro farm “Yuzhnaya” of Temruk in the Krasnodar Region. Melaphen is a type of melamine salt of bioximethilphosphin acid. It evokes complex influence on the energetic complex of vegetation cell in all three stages of vegetation. The chemical was applied mechanically by a tractor sprinkling. The square of tested patch is 2 hectares. Melaphen usage was 6,7 ml to 1 hectare . Norm of liquid applied was 700 liters to 1 hectare. Three times the chemical was applied during vegetation: before blossoming, grape growth phase (grapes the size of a pea) and before the grapes ripen. As a result it was estimated that three times chemical application of melaphen to Sovinion White in dose 1. 10-9 in period after blossoming of grapes and before the grapes ripen led to more saturated accumulation of sugar in juice of the grapes and also to faster ripening and harvesting, larger mass of grape bunch amount of harvesting from a bush. Besides, in the tested option growing processes are accelerated and pigmentation agents are accumulated faster in the leaves and embryo infestation in winter sleeping core is boosted at larger scale. Better harvest quality leads to better wine materials quality. Application of melaphen provides higher economical efficiency

There was given the review of the results of the study in the conditions of the Anapa-Taman zone of the Krasnodar region of the influence of the treatment of vines of Saperavi of lignohumates of the grade "В" (“LG-B Bio”, “LG-B Best Bio”, “LG- Best L”) on the quality of must and wine materials. The technology of the grape cultivation on the experimental plot corresponded to the adopted technology which is used in the JSC "Pobeda" of Temryuk District and was widely accepted for the keeping of fruit-bearing plantations of the zone of uncovered viticulture. Agrobiological works were carried out at the optimum terms and were of high quality. Vines of the third- year of life, embodied by the scheme in 3,0 x 1,5 m. The forming is a one-sided Guyot with a height of the trunk in 60 cm. On bushes there was formed the same load shoots and clusters. There were carried out the treatments of grape leaf surfaces by the solutions of lignohumates twice: the 1st – before the flowering and the 2 nd in the beginning of the formation of berries (in 20 days after the first one). The spraying was carried out in the early morning hours. The accounting of the grape harvest and the sampling for the determination of the quality of must and wine materials (10 kg per each type) was conducted in September 20, 2012. The technological and physical-chemical analyses were carried out in the shop of mini winemaking and in the accredited testing laboratory of the grape processing of the scientific center of the winemaking SCSRIHGG of the RAAS. The preparations "LG-B Bio", "LG-B Super Bio" and "LG-B Best A" as grape growth regulators have the equal and stable effect

This article presents the possibilities of using organic fertilizers, which allow you to recharge grape raw material with biologically active substances and improve the nutritional value of wine production. The influence of organic fertilizers on activation of process for detoxification of soil toxic compounds has been measured, ensuring food safety of raw materials for the production of grape wine products by reducing of hazardous chemicals. The use of biotechnology preserves and also enhances the uniqueness and diversity of the chemical composition of the grape raw material. We have established that after the four-year application of biotechnology the content of toxic compounds in grape raw material was reduced by 7%, and the concentration of biologically active substances increased in 1,5-2% times in comparison with the production of commercial plantations

The wide variety of microorganisms has been identified in many wine-making countries on the berries of grapes. These are yeasts of different families, forms and kinds, bacterium, mold fungi. In the article, we present the results of investigating species composition of microflora of berries of white and red types of grape, which grows in different economies of the Krasnodar region and the republic of Abkhaziya. The sowings onto the elective media were conducted for the development of entire spectrum of yeast. The grown colonies after preliminary microscoping were separated into the cultures and subjected to testing according to the culturalmorphological signs, being guided by determinants and benefits. It was established the specific variety of microflora on the surface of the berries of grapes of all investigated types, without dependence on the place of their growth. Obtained data showed that the group of yeast, which constantly is present in the complex of the epiphytic microorganisms of grapes of Saccharomyces, Pichia, Hansenula, Hanseniaspora was characteristic for all types of grapes in all investigated regions. The heterogeneity of the taxonometric composition of microflora is shown. Prevailed yeasts were of family Saccharomycetaceae, form Saccharomyces vini. A quantity of yeast of Saccharomyces vini decreases in a number of Myskhako-Caucasus-Fanagoriya, that as a whole will be coordinated with the climatic conditions. Only the type of Pinot nuar grapes had yeasts of Brettanomyces Dekkera. On the berries of Cabernets and Karaburnu we have discovered yeasts of Schisosaccharomyces acidodevoratus, causing acid-reduction. On the berries of the grapes, which grew in joint stock company APF “Fanagoriya” we haven’t revealed the presence of lactic acid bacteria Lactobacillus brevis and yeasts of the form of Schisosaccharomyces acidodevoratus. In the same farm the smallest quantity of yeastswreckers is noted, which we the forms of Pichia and Candida. In the microflora of Cabernets, Risling and, especially, Karaburnu grapes, we have found lactic acid bacteria Lactobacillus plantarum and Lactobacillus brevis. Lactobacillus plantarum. Among the bacteria the total quantity of coccic flora composes - 56 %, the rod-shaped - 44 %. Two types of active acetous bacteria are identified – Acetobacter aceti and Acetobacter xelinum. Especially high was their surface concentration on the berries of Kachich type of grapes