Name
Rebrov Anton Nikolayevich
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
Russian national scientific research institute of wine growing and winemaking of Y.I.Potapenko
Web site url
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Articles count: 5
The article presents the results of microclonal reproduction and recovery of native grape varieties, the creation of basic nursery. In addition to the culture of apical meristems, we have developed a chemotherapy using salicylic acid. A method of decontamination of plants from mycoplasma infection has also been developed, which includes the introduction of the antibiotic Cefotaxim in the nutrient environment at a concentration of 50 to 450 mg/L, depending on the degree of infection of plants. Adding to the nutrient medium of the drug Emistim reduces the death of meristems from infection by 3-5 times, improves their differentiation. The use of the drug Melafen helps to improve morphogenesis and quality characteristics of plants. The high survival rate of meristems during adaptation to non-sterile conditions has been noted. Biological testing on herbaceous indicators showed no chronic diseases. Plants after adapting to non-sterile conditions are planted, in the form of vegetative seedlings with a closed rootsystem, on the basic nursery. The survival rate of plants was 70-80%, in some varieties it is higher: 96.4% (Sypun black) - 98.6% (Krestovsky). It has been proved that in vitro clonal microbreeding there is no change at the genetic level even after 8 years of cultivation, which confirms the reliability of the developed technology of reproduction and recovery of native grape varieties in vitro
In this article we present information on features of
microcloning in culture of in vitro of perspective for
Eurasia, pink and white-berry table genetic grades of
grapes by I. A. Kostrikin, V. N. Kraynov and V. V.
Zagorulko. Researches were conducted at an input
stage in culture of in vitro of meristems of 0,1-0,2 mm
in size. At the majority of grades and forms, we noted
good regeneration abilities in the conditions of sterile
culture. It is bound to the fact that almost all the
studied grades: Anyuta, Bogatyanovsky, the Princess
Olga, Preobrazhenie, Anniversary of Novocherkassk,
Favor are received from crossing of couple the Mascot
× Sultana-ray grape. And only grades called Libya and
Lowland which showed lower regeneration properties
at stages of proliferation and rooting of shoots are
received from crossing the couple of Flamingo ×
Arkadya and (Talisman x Tomaysky) respectively. At
the same time, the grade of Talisman, being one of the
parents of the majority of the studied grades, showed
stable results at all stages of cultivation
Results of research of influence of foliar top dressings by Zitovit and Zircon preparations on development of plants of grape of Berlandieri × Riparia Kober 5Ð’Ð’ grade on sandy soils are submitted. Processing of plants with Zitovit in conditions of their insufficient mineral nutrition through roots, improved their growth and development. Action of Zircon preparation for raising immunity promoted qualitative rearrangement of morphological parameters of development of plants
The generalized long-term experience on creation of base of Queen cells in grape varieties, improved in vitro culture, on the sandy array of Ust-Donetsk district of Rostov region. Problems frequently encountered on sandy soils and ways of their solution are shown. We focus on: rehabilitation of the landing of plants of grapes in the conditions of greenhouses, open ground, the optimum time and methods of planting. The work shows the effectiveness of different dressings and types of fertilizers, influence of drugs of new generation, affected the uterine protection of plantations from pests and disease, deep sandy soils freezing in winter, especially the development of root system of different varieties. On the basis of generalization of long-term data, we suggest the most appropriate solutions, and developed technology bookmarks and conducting basic queen cells from healthy in vitro planting material of grapes in the sandy array