Scientific Journal of KubSAU

The article reports in color on the characteristics of the three varieties and their clones that are common in Afghanistan. In the process of ampelographic screening of vineyards in Afghanistan, three varieties previously undescribed in Russian literature under the local names Aita, Gundyan and Shandyhani were discovered. Their identification by ampelographic and ampelometric characteristics made it possible to establish the original Aita variety, the Gundyan variety as a synonym for Guladan and Shandyhani as a synonym for the world famous Sultanin, or Kishmish. The described varieties are quite common in Afghanistan, reputable in economic returns and are still promising for cultivation in their country, where they are successfully used in the manufacture of dried products and high-quality diet juices. To identify the impact of various environmental conditions on the growth, development, quantity and quality of the crop, these varieties must be tested in all zones of different ecological and geographical areas of cultivation

On the example of economic analysis, we have characterized the life cycle of a scientific institution, the history of the formation and development of which covers almost a three-hundred-year period. The article determines features of the methodology for identifying the stages of the life cycle of a scientific organization and identifying factors that determine the stage of the life cycle

The article presents the results of microclonal reproduction and recovery of native grape varieties, the creation of basic nursery. In addition to the culture of apical meristems, we have developed a chemotherapy using salicylic acid. A method of decontamination of plants from mycoplasma infection has also been developed, which includes the introduction of the antibiotic Cefotaxim in the nutrient environment at a concentration of 50 to 450 mg/L, depending on the degree of infection of plants. Adding to the nutrient medium of the drug Emistim reduces the death of meristems from infection by 3-5 times, improves their differentiation. The use of the drug Melafen helps to improve morphogenesis and quality characteristics of plants. The high survival rate of meristems during adaptation to non-sterile conditions has been noted. Biological testing on herbaceous indicators showed no chronic diseases. Plants after adapting to non-sterile conditions are planted, in the form of vegetative seedlings with a closed rootsystem, on the basic nursery. The survival rate of plants was 70-80%, in some varieties it is higher: 96.4% (Sypun black) - 98.6% (Krestovsky). It has been proved that in vitro clonal microbreeding there is no change at the genetic level even after 8 years of cultivation, which confirms the reliability of the developed technology of reproduction and recovery of native grape varieties in vitro

The article reveals analysis of varietal and age structure of vineyards occupied by 29 varieties and clones of the technical direction of use in the branch "Alushta" of FSUE "PJSC "Massandra". Taking into account the ecological and climatic conditions of the Alushta valley and the specialization of the economy, the largest share in the assortment of technical varieties is occupied by red grape varieties: Cabernet Sauvignon and clones of Cabernet Sauvignon R5, R8, VCR8, No. 169 including (26.68%), Bastardo Magarachsky and Bastardo clone VCR1 (12.06%), Saperavi (7.33%), Krasnostop zolotovsky (5.69%), Merlot and clone Merlot R3 (4.62 %). White varieties are in a smaller percentage: white Muscat and white Muscat clone R3 (8.47 %), white Kokur (7.1%), Aligote (3.82%), Semillon and clones Semillon â„– 173 and â„– 299 (3.68%), Gars level (2.99%). Plantings in the most productive age of 6-10 and 11-15 years are 57.5 % of the total area. At the same time, the share of introduced clones of varieties accounts for 26.65 %. In General, the age structure of the vineyards is acceptable. The company produces high quality wine materials for production of traditional Federal state unitary enterprise "PJSC "Massandra" wines: white Port Alushta, Alushta red Port, pink Port Alushta, Alushta red Table, Pinot Gris, Massandra, Madera Crimean Alushta Tokay, white Muscat, Massandra Kokur, Semillon Alushta and sherry wine. In addition, there are new brands of semi-sweet white and red wines Pearl Massandra, as well as varietal wine materials Aligote, Cabernet, Sauvignon, Merlot, Saperavi, Chardonnay, Bastardo and Semillon. Analysis of the assortment shows that in the production plantings of "Alushta" branch of FSUE "PJSC "Massandra" there are varieties of medium and late ripening, which does not create peaks during harvesting and processing, ensuring uniform flow of raw materials for winemaking. It is necessary to expand the assortment of the economy due to a number of promising varieties of Syrah, Petit Verdot, Malbec, Sangiovese and others with a complex of economically valuable properties introduced from other regions

The article presents the results of the fertilizers use effect on dry wine materials produced from the Chardonnay variety, grown in the Anapo-Taman soil and climatic zone of the Krasnodar region, Russia. As a result of wine materials analysis, increase of tartaric acid was noted, with the autumn application of phosphorus-potassium fertilizers and the spring application of ammonium nitrate. At the same time, its optimum content was noted with nitroammophos. Also, the minimum concentration of malic acids was detected by the application of nitroammophos with potassium fertilizers. In its turn, early spring application of ammonium nitrate reduced the concentration of malic acid below the control sample (without fertilizers). It was revealed that the use of mineral fertilizers in this winemaking zone also contributed to a reduction of lactic acid in the must. At the same time, the application of nitroammophos (especially together with other mineral fertilizers), contributed to the accumulation of citric and succinic acids, as well as phenolic compounds. Moreover, the application of phosphate-potassium fertilizers in the autumn contributed to the accumulation, and, consequently, also to the increase in the content of phenolic compounds in the resulting must. In addition, the studied samples of wine materials contained high alcohol concentrations and low concentrations of sugars with volatile acids. At the same time, the maximum amount of alcohol exceeding its content in the control sample was revealed when phosphorus-potassium fertilizers were used together with nitroammophos fertilizers. It is also noted that the application of ammonium nitrate did not affect this indicator. As a result, the use of mineral fertilizers in the studied concentrations made it possible to prepare wine materials that contained a low concentration of sulfur dioxide, which is substantially lower than the harmful concentration for humans. In addition, increased concentrations of hydrogen ions in variants with the application of phosphate-potassium fertilizers have made it possible to reduce the number of iron tanates and the intensity of oxidation-reduction processes

The analysis of varietal and age structure of vineyards occupied by 29 varieties and clones of the technical direction of use in the "Alushta" branch of FSUE "PJSC "Massandra". Taking into account the ecological and climatic conditions of the Alushta valley and the specialization of the economy, the largest share in the assortment of technical varieties is occupied by red grape varieties: Cabernet Sauvignon and clones of Cabernet Sauvignon R5, R8, VCR8, No. 169 including (26.68%), Bastardo Magarachsky and Bastardo clone VCR1 (12.06%), Saperavi (7.33%), Krasnostop zolotovsky (5.69%), Merlot and clone Merlot R3 (4.62 %). White varieties are in a smaller percentage: white Muscat and white Muscat clone R3 (8.47 %), white Kokur (7.1%), Aligote (3.82%), Semillon and clones Semillon â„– 173 and â„– 299 (3.68%), Gars level (2.99%). Plantings in the most productive age of 6-10 and 11-15 years are 57.5 % of the total area. At the same time, the share of introduced clones of varieties accounts for 26.65 %. In general, the age structure of the vineyards is favorable. The company produces high quality wine materials for production of traditional Federal state unitary enterprise "PJSC "Massandra" wines: white Port Alushta, Alushta red Port, pink Port Alushta, Alushta red Table, Pinot Gris, Massandra, Madera Crimean Alushta Tokay, white Muscat, Massandra Kokur, Semillon Alushta and sherry wine. In addition, there are new brands of semi-sweet white and red wines Pearl Massandra, as well as varietal wine materials Aligote, Cabernet, Sauvignon, Merlot, Saperavi, Chardonnay, Bastardo and Semillon. Analysis of the assortment shows that in the production plantings of "Alushta" branch of FSUE "PJSC "Massandra" there are varieties of medium and late ripening, which does not create peaks during harvesting and processing, ensuring uniform flow of raw materials for winemaking. It is necessary to expand the assortment of the economy due to a number of promising varieties of Syrah, Petit Verdot, Malbec, Sangiovese and others with a complex of economically valuable properties introduced from other regions

Intensive viticulture as a sub-branch of the grape-wine industry of agriculture is developing due to the introduction of modern scientific developments. The intensification of production consists of laying vineyards with planting material of higher biological categories, highly productive clones of traditional and long-established selection varieties; advanced agrotechnological techniques and mechanized operations, etc. For import substitution of table grapevine in our country, it is especially important to introduce new big-berry varieties from early to late maturity, providing a continuous 100-day conveyor for harvesting, with a bunch of grapes, various colors and the shape of berries. In many countries of the world with developed viticulture, breeding programs are being conducted to create competitive table varieties. As a result, there were obtained Muscat of Italy (Italy), Cardinal, Red Globe (USA), Moldova (Moldova), widely spread all over the world. But the world market does not stand still and breeders are working on new varieties. In recent years, there has been a trend of enlargement of berries from 8 to 24 grams, preference is given to varieties with pink and red berries. So, for example, created in Ukraine - a very early variety of Livia; in Russia - the variety clones of Livia K and Rochefort K, the early varieties of the Memory of the Teacher, the Gift of the Nesvetay, the Preobraghenie and the Jubilee of Novocherkassk, and others. These varieties are bred according to the developed model, which shows the genetic regularities of the formation of maximum expression in transgressive recombinants in quantitative (mass of berries and bunches) and qualitative (shape and color of berries) traits associated with maturation

The generalized long-term experience on creation of base of Queen cells in grape varieties, improved in vitro culture, on the sandy array of Ust-Donetsk district of Rostov region. Problems frequently encountered on sandy soils and ways of their solution are shown. We focus on: rehabilitation of the landing of plants of grapes in the conditions of greenhouses, open ground, the optimum time and methods of planting. The work shows the effectiveness of different dressings and types of fertilizers, influence of drugs of new generation, affected the uterine protection of plantations from pests and disease, deep sandy soils freezing in winter, especially the development of root system of different varieties. On the basis of generalization of long-term data, we suggest the most appropriate solutions, and developed technology bookmarks and conducting basic queen cells from healthy in vitro planting material of grapes in the sandy array

The article presents the structural and phylogenetic analysis of VIT_02s0033g00410, VIT_02s0033g00390 and VIT_02s0033g00450 genes of the grapevine genome and the closely related orthologous gene MYB114 of the Arabidopsis genome. These genes are responsible for the biosynthesis of anthocyanin in the organs of model plants and are of interest not only for practical production and breeding, but also for fundamental research. These genes were analyzed for GCcomposition of nucleotides, the presence of cisregulatory elements and promoter regions. DNA and protein sequences were aligned to look for similar elements, which allowed further analysis of the ultraconservative domains of four genes. Based on the results of search and identification of the conservative regions, a cluster tree was constructed, which made it possible to identify the separation of gene sidelines from, presumably, the main one. At the same time, the construction of consensus trees based on DNA and protein sequences revealed their absolute similarity. "The Minimal Evolution Tree" allowed calculating the approximate dates of the appearance of the mutations and the divergence times of the gene branches between each other. At the same time, the appearance of the Vitis genus and its separation from the Rosales was taken as the time first divergence point. In the end, homologous metabolic pathways were searched between grapevine and Arabidopsis, which revealed the presence of homologous proteins in the grape proteome. In this turn, it already confirms the existence of similar biosynthetic pathways and, as a consequence, interactions such as "DNA-protein" and "protein-protein"

The influence of growth, its ripened part and the diameter of the vine at the base of the shoot on the growth power of 922 seedlings of 55 combinations of crosses in 2011 and 2012 was studied (planting scheme 3 x 1 m), seedlings of grapes of the second and third years of vegetation in the field conditions of the Southern coast of Crimea. The mean vine maturation for all populations was 64.7%, the diameter at the base of the shoots was 3.3 mm. After pruning to reverse growth, in the third year of vegetation of seedlings (2014), the length of growth of shoots increased significantly and the average for populations it became 73.3 cm. The coefficient of variation increased, 26%, - it acquired the character of a significant deviation from the general population. This happened because the seedlings, which had a vine growth less than 25 cm in the second year of vegetation, had a growth of only 35 cm in the third year, whereas in all seedlings that had a growth of more than 30 cm, in the following year shoots increased almost 1, 5 and 2 times more. Formulas are proposed for estimating the growth force of seedlings according to the 9-score scale OIV (OIV)