Scientific Journal of KubSAU

The clone selection has very outstanding position behind the actual problems of modern viticulture in the world and in our country. This problem usually has the primary importance behind the scientist who works with grape in all the countries such as USA, German, French, Spain, Italy, Hungary, Bulgaria, Moldavia, Ukraine and etc. In our country last years some positions becomes solving in the plane of scientific research work. Consequently, in the article the history and the situation of clone selection of grape on the Eurasian continent is widely covered.

Step by step results of many year researches of clonal selection of grape varieties: Clairette white, Saperavi, Sauvignon white and Chardonnay, growing in the Ltd company “ Pobeda” of Temryk district of Krasnodar region are cited in the article. Highly productive proto clones were selected. Clairette white is the best one as a variety from them and it was given to the state experiment under the name Clairette temryk.

On the example of economic analysis, we have characterized the life cycle of a scientific institution, the history of the formation and development of which covers almost a three-hundred-year period. The article determines features of the methodology for identifying the stages of the life cycle of a scientific organization and identifying factors that determine the stage of the life cycle

This article presents information about the features of biotechnology as the driving force of scientific and technological progress. The national programs of the leading countries of the world, it is one of the priority sectors, reflecting the level of the socio-economic condition of the society. Biotechnology is now successfully solves such vital tasks as providing food, the establishment of effective medicaments, obtaining fuel based on renewable raw materials, maintaining ecological balance, conservation of biological resources of the Earth. The development of agriculture in modern conditions is impossible without agricultural biotechnology. It is directly related to viticulture. Choosing an object of an integrated system (embryos, apical meristem, axillary buds), it is possible to clone plants, i.e. produce plants identical to the original. If the same as the object to use isolated cells or protoplasts, in this case, there will most likely altered versions, creating diversity for the breeder. Genetic engineering – the science of younger, since the establishment of the first chimeric DNA molecule. The origin of genetic engineering is rooted in the development of molecular genetics, biochemistry. These technologies, undoubtedly progressive, but their biological safety is still insufficiently explored and is a danger to all life on Earth. The leading Western powers carried out strict control over the introduction of transgenic crop plants, as they are in agrocenosis new biological risks that may adversely affect the plants, animals and humans. In Russia, as in other countries, have already adopted the law “State regulation of genetic engineering”

The article presents the results of microclonal reproduction and recovery of native grape varieties, the creation of basic nursery. In addition to the culture of apical meristems, we have developed a chemotherapy using salicylic acid. A method of decontamination of plants from mycoplasma infection has also been developed, which includes the introduction of the antibiotic Cefotaxim in the nutrient environment at a concentration of 50 to 450 mg/L, depending on the degree of infection of plants. Adding to the nutrient medium of the drug Emistim reduces the death of meristems from infection by 3-5 times, improves their differentiation. The use of the drug Melafen helps to improve morphogenesis and quality characteristics of plants. The high survival rate of meristems during adaptation to non-sterile conditions has been noted. Biological testing on herbaceous indicators showed no chronic diseases. Plants after adapting to non-sterile conditions are planted, in the form of vegetative seedlings with a closed rootsystem, on the basic nursery. The survival rate of plants was 70-80%, in some varieties it is higher: 96.4% (Sypun black) - 98.6% (Krestovsky). It has been proved that in vitro clonal microbreeding there is no change at the genetic level even after 8 years of cultivation, which confirms the reliability of the developed technology of reproduction and recovery of native grape varieties in vitro

The polymorphism is the diversity of varieties in ampelografy - the presence simultaneous permanent in the population of two or several phenotypic and genotype differing groups of plants of one variety – i.e. clones. In viticulture the isogenic population is usually called “clone” and variety as the total of clones or forms – i.e. it is heterogenic. Polymorphism of varieties has big practical sense, as varieties-clones have the harmonic complementary adaptive ampelocenosis and higher economical effect for production. In the article the polymorphism of two heterogenic population - the group Pinot and Riesling, which are the best specimen of French and German viticulture is described on the phenotypic level of leaves

The article contains results of the study of biochemistry of some perspective table grape varieties: Aniuta, Vodograi, Nizina and Jubilee Novocherkasska, yield in 2013 from the Central zone of Krasnodar region. At the time of redy yield the combination of sweetness and acidity of fruits were favorable. The ratio of fructose to glucose was 1.69; 1.36; 2.00; 1.13, respectively, in grapes Aniuta, Vodograi, Nisina, Jubilee Novocherkasska. According to the percentage of fructose in fruit juice varieties Aniuta, Vodograi and Jubilee Novocherkasska close to each other (50.93; 56.14; 51.23%). According to the percentage of glucose similar varieties Aniuta and Nizina (33 - 34.27%), Vodograi and Jubilee Novocherkasska (41.16 - 45.22%). Sort Jubilee Novocherkasska, had content in berries close fructose and glucose. Most of fructose (66.02%) in berries variety Nizina. In berries studied cultivars organic acid content was: wine 3128 - 3861, malic 982 - 3753, citric 109 - 317, amber 3 - 115 mg / dm3. The highest concentrations of potassium and sodium cations are presented in berries varieties Aniuta: potassium - 1219 sodium - 35.13 mg / dm3 and cations magnesium and calcium in berries Jubilee Novocherkasska 46.75 and 73.64 mg / dm3. The lowest concentration of potassium and sodium cations observed in grape Vodograi, magnesium - from grape Aniuta, calcium - at grape Nizina. We studied grape bunches are large, not dense, beautiful, berries are large, harmonious taste. Tasting score fresh grapes varieties Aniuta, Vodograi, Nizina, Jubilee Novocherkasska 7.6; 7.4; 7.8; 7.8 points respectively

The article highlights the biochemical characteristics of perspective newest varieties of table grapes and clones of the Kuban region; it also presents the results of the study in an industrial environment of OAO AF "South", OOO "Phanagoria-Agro", Training Farm "Kuban" and branches of the Department of Viticulture of Kuban State Agrarian University and in Farmer-amateur conditions of private entrepreneurs of Kuban

Introduced grape varieties such as Ancelotti, Bachka, Johanniter, Morava and Solaris which were grown in the conditions of Taman provided production of high yields of grapes with standard conditions of sweetness and acidity of grape juice that were useful for the technical processing of not only tableware, but also for special wines, in particular, dessert. Organoleptic properties of all the studied wine materials from grapes of these varieties were of high quality, which was con-firmed by their tasting evaluation. They are recom-mended for the preparation of organic (bio) wines. The "Biologik" wine in 2012 received a certificate of the high points at the international exhibition in Italy (Rome), and the wine from red grape variety Ancelotti for several years, winning awards from international wine competitions ("Yalta. Gold Griffon", "Gold au-tumn")

Grape variety assembly lines by zones of Krasnodar region worked out by the staff of the chair of grape growing on the basis of many years data and with an account of really existing areas of every variety by the end of 2007 have been presented in this article. Assembly lines allow to plan more balanced entering of grape production to the processing enterprises or to a consumer market.