Scientific Journal of KubSAU

The article presents results of studying the oenology and the biochemistry of the Cabernet-Sauvignon grapes in different areas of the Krasnodar region - Taman, Anapa, Abrau-Dyurso, Gelendzhik and Krasnodar. The yields, the mechanical composition of the grapes, the biochemical indicators of the juice of berries were compared. The cultivation zone affects the yield, the average mass of the bunch, the number and mass of berries in the bunch, the mass of the crest, but according to the percentage of berries and crest in the bunch, the bunch structure (ratio of mass of berries to mass of the ridge) does not significantly differ between growing zones. The mass of 100 berries and 100 seeds, the weight of seeds in 100 berries, the clustering index (the ratio of pulp with juice to peel) can differ in different places of growth, but with insignificant coefficients of variation. The composition of the bunch as a percentage of the total mass varies little over the growing zones: the ridges make up 5.6- 5.7%, the skin 4.9-5.2%, the seeds 4.8-4.9%, the pulp with the juice - 84.3-84.5%. Structural and berry indicators varied in the range of 5.4-5.5 and 76.84, respectively. Depending on the place of cultivation, the yield of the grape must in laboratory conditions was 75.1-75.7%. Analysis of the structure, composition and the structure of bunches showed that the mechanical composition of Cabernet-Sauvignon refers to the first group of varieties that retain their valuable characteristics when changing the growing conditions. When harvesting, the mass concentration of sugars was 19-19.8%, titrated acidity - 8.1-9.2 g / dm3, pH - 3.3- 3.4. Depending on the place of cultivation of the content of organic acids in the juice of berries was: wine - 4917-5918 mg / dm3, apple - 1926-2763, lemon - 215-416 mg / dm3. In different places of cultivation in% of the total amount of organic acids studied, the content of tartaric acid varied from 61.7 to 71.4%, apple - from 24.7 to 33.3%, lemon - from 3 to 5%. Thus, the content of organic acids in the juice of Cabernet-Sauvignon berries depends on the specific growing conditions. Depending on the growing places, the mass concentration of potassium cations is 2630-3508 mg / dm3, sodium 162-436, magnesium 171-230, calcium 185-255 mg / dm3. However, in most cases, as a percentage of the total cation content, there are no differences depending on the site of growth. Thus, the biochemical parameters of the juice of CabernetSauvignon berries depend on the specific natural conditions of the terrain and, according to these parameters, the variety belongs to the second group of varieties with less plasticity to different growing conditions

The article discusses the application of automated system-cognitive analysis (ASC-analysis), its mathematical model is a system of information theory and implements, its software tools – intellectual system called "Eidos" for solving one of the important tasks of ampelography: to quantify the similarities and differences of different clones of grapes using contours of the leaves. To solve this task we perform the following steps: 1) digitization of scanned images of the leaves and creation their mathematical models; 2) formation mathematical models of specific leaves with the application of information theory; 3) modeling the generalized images of leaves of different clones on the basis of specific leaves (multiparameter typing); 4) verification of the model by identifying specific leaf images with generic clones, i.e., classes (system identification); 5) quantification of the similarities and differences of the clones, i.e. cluster-constructive analysis of generalized images of leaves of various clones. The specific shape of the contour of the leaf is regarded as noise information on the clone to which it relates, including information about the true shape of a leaf of this clone (clean signal) and noise, which distort the real shape, due to the random influence of the environment. Software tools of ASA-analysis which is intellectual "Eidos" system provides the noise suppression and the detection of a signal about the true shape of a leaf of each clone on the basis of a number of noisy concrete examples of the leaves of this clone. This creates a single image of the shape of the leaf of each clone, independent of their specific implementations, i.e. "Eidos" of these images (in the sense of Plato) - the prototype or archetype (in the Jungian sense) of the images

The article highlights the issues of grape plants resistance to low temperatures; it is an urgent problem for all wine-growing regions of the world, located in the zone of risky agriculture. Losses caused by frost, indicate long-standing necessity of perfection of assortment in terms of enhancing its hardiness by enriching more resistant genotypes obtained both through the introduction or naturalization, as well as from the crossing of indigenous with interspecies hybrids. Methodology for evaluating frost resistance properties of the material served as a preparation for the originality of his research and experimental evaluation of the unique divisibility properties of the aim. Thus, we initially conducted hardening of matured cuttings at positive temperature plus 8 plus 4 ° C for 14 days (I quenching phase) and a negative temperature minus 5 ° C-minus 7 ° C for 9 days and minus 10 ° C-1 day (II hardening phase). Then the cuttings we frozen in the temperature range from minus 16 ° C to the next steps of consistent freezing (minus 16 ° C - 2 days; minus 18 ° C - 2 days; minus 20 ° C - 2 days; minus 22 ° C – 1day, minus 24 ° C - 1 day, minus 26 ° C - 1 day, minus 28 ° C - 1 day and minus 30 ° C - 8 hours). After freezing of cuttings of each variety, the sample was placed on the 3 days in the refrigerator at the temperature of + 2 ° C to their gradual thawing. Cuttings were grown at room temperature on water. As a result, obtained by the accounting: the lowest frost-resistance among the studied forms of parental Crimean origins was found in grades of Shabash, Soldayya and Solnechnodolinsky; the varieties of Dzevat kara, Kokur bely, Kokur cherny, Misket, Misgyuli kara, Sari Pandas were set as a weak level (-20 °C); Aybatly, Kefesiya, Kok pandas and Ekim kara have average level up to -22 °C; the highest frost-resistance - 24 °C – was shown by Khersonessky and Kapselsky. Also, we have identified synthetic frost-resistant F1-population and plus-transgressive selection form

The article discusses the use of automatic systemic-cognitive analysis (ASC-analysis), its mathematical model is a system of information theory and software tools – an intellectual system called "Eidos" for the solution of some problems of ampelography: 1) digitization of scanned images of the leaves and creation of their mathematical models; 2) the formation of mathematical models of specific leaves using the spreading of information theory; 3) the formation of models of generalized images of leaves of various sorts; 4) comparing an image of a specific leaf with a generalized image of the leaf of different varieties and finding a quantitative degree of similarity and differences between them, i.e. the identification of the varieties on the leaf; 5) quantification of the similarities and differences of the varieties, i.e. cluster-constructive analysis of generalized images of the leaves of different varieties. We propose a new approach to digitizing images of leaves, based on using the polar coordinate system, the center of gravity of the image and its external contour. Before scanning images we may use transformation to standardize the position of the still images, their sizes and rotation angle. Therefore, the results of digitization and ASC-analysis of the images might be invariant (independent) relatively to their position, size and rotation. The specific shape of the contour of the leaf is regarded as noise information on the variety, including information about the true shape of the leaf of the class (clean signal) and noise, which distort this true form, originating in a random environment. Software tools of ASC-analysis – intellectual "Eidos" system ensures noise reduction and the selection of the signal about the true shape of the leaf of each variety on the basis of a number of noisy concrete examples of the leaves of this variety. This creates a one way form of a leaf of each class, free from their concrete implementations, i.e., the "Eidos" of these images (in the sense of Plato) is a prototype or archetype (in the Jungian sense) of the images

It is well known that genetics studies the mechanisms of variation/heredity and widely uses the concept of "genetic information". While genetics considers the information as the content of the genetic code - structure of DNA and RNA included in the cell of a living organism. Genetics examines the mechanisms of recording, copying, readout of genetic information, the possibility of its modification and its influence on the characteristics and properties of the organism. In conversational and scientific language we know phrases, such as "Genes contain information about the characteristics/properties of the body." Paradoxically, we see no attempts to determine the amount of information contained in specific genes on specific characteristics or phenotypic properties of the organism. It would seem that the application of information theory in genetics is a completely natural and suggests itself. More strange that there are practically no works devoted to the application of information theory for solving problems of genetics. This article is intended, to some extent, to fill this gap on the example of calculating the amount of information in the genes of the characteristics or properties of different grape varieties. It examines the application of automated system-cognitive analysis (ASC-analysis), its mathematical model – system of information theory and software tools – intellectual system called "Eidos" for solving one of the important tasks of genetics: determine the amount of information contained in the genes on various phenotypic characteristics/properties of the grapes. To solve this problem, we perform the following steps: 1) cognitive-targeted structuring of the subject area; 2) the formalization of the subject area, i.e. development of classification and descriptive dials and graduations and training samples; 3) synthesis and verification of information model, reflecting the amount of information in the genes on the phenotypic characteristics/properties (multiparameter typing); 4) displaying the information about the genetic determination system of phenotypic characteristics/properties (SWOT analysis of Fennovoima); 5) displaying the information about the strength and direction of influence of a specific gene on phenotypic characteristics/properties (SWOT-diagrams of genes); 6) the solution to the problem of system identification phenotypic characteristics/properties by the presence of certain genes; 7) quantification of the similarities-differences of the various phenotypic characteristics/properties, upon determination system genes. A specific phenotypic property (or characteristic) is regarded as a noisy genetic text, including genetic information about the true gene property (clean signal) and the noise that distorts this information due to the random effects of the environment. The software tool of the ask-analysis which is "Eidos" intellectual system provides the noise suppression and the selection of true signal

In this article we present information on features of microcloning in culture of in vitro of perspective for Eurasia, pink and white-berry table genetic grades of grapes by I. A. Kostrikin, V. N. Kraynov and V. V. Zagorulko. Researches were conducted at an input stage in culture of in vitro of meristems of 0,1-0,2 mm in size. At the majority of grades and forms, we noted good regeneration abilities in the conditions of sterile culture. It is bound to the fact that almost all the studied grades: Anyuta, Bogatyanovsky, the Princess Olga, Preobrazhenie, Anniversary of Novocherkassk, Favor are received from crossing of couple the Mascot × Sultana-ray grape. And only grades called Libya and Lowland which showed lower regeneration properties at stages of proliferation and rooting of shoots are received from crossing the couple of Flamingo × Arkadya and (Talisman x Tomaysky) respectively. At the same time, the grade of Talisman, being one of the parents of the majority of the studied grades, showed stable results at all stages of cultivation

The article presents the results of studies of 92 indigenous varieties and 15 wild genotypes by 24 microsatellite markers. As a result a locus database about germplasm in the collection stored at the Russian ampelographical collection AZOSViV (Anapa Zone Experimental Station of Viticulture and winemaking) was created. During the research of varietal breeding populations confirmed the validity in the production of the cultivar of Krasnostop anapsky with obvious changes in microsatellite sequences as a clone of famous indigenous Don cultivar Krasnostop Zolotovskii. This clone was selected in 1953 by prominent agrotechnologist Zotkin Ivan Ivanovich which is propagated by the author and his followers, and after a formal registration submitted to state testing in 2007 in GSK RF, zoned in the North Caucasus region (number 6) in 2014 year. Sort -clone Krasnostop anapsky only in the Kuban today cultivating in 35 hectares. By using molecular genetic techniques, now clonal selection of vine grapes is reasonably improving. Recent data collected by us in the work process, were analyzed a comprehensive manner, making it possible to draw conclusions about distinctness of grape varieties. Some clones were studied by DNA primers, based on retrotransposon sequences

Clonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way

Numerous studies have found a different quality of the buds along the length of the fruit arms, which expressed various degrees establishing embryonic inflorescences in the central buds. It is revealed that many of the grapes have more fruit-bearing eyes formed on average (4-6 knots) and upper (7 to 10 knots) levels of fruit escape, compared to the lower (1-3 nodes) layer. In connection with the different quality of the buds along the length of fruit vines should be used and different ways of cutting them. It is necessary to consider the biological characteristics of varieties, ways of doing and shaping the bushes, the weather conditions of the area during the growing season and relative calm. To receive annually a stable high yield of grapes, we must first set the optimal length of pruning fruit shoots and optimal load on the Bush healthy eyes. This requires for each class on the eve of trimming bushes to carry out the analysis of the wintering holes along the length of the fruit shoots and to identify the embryonic fruitfulness of Central buds, i.e. to carry out the prediction of grape yield next year. The article presents data on the formation of the embryonic fruitfulness of Central buds of wintering buds at the Taman group of grapes – Arcadia, Viorica, Hibernal, Cabernet Sauvignon, Merlot, Moldova, Pinot white, Riesling, Saperavi, Traminer pink, Tsimlyansky black, Chardonnay. Found higher rates of embryonic fruitfulness of Central buds buds in the studied grape varieties - Chardonnay, Riesling, Viorica, Hibernal, Merlot, Moldova, where the coefficients of fruiting ranged from 1.40 m from Riesling to 1.69 from Merlot; factor productivity of 1.58 from the Chardonnay to 1.77 from Merlo. The high percentage of fruitful buds from 80,8 from Cabernet Sauvignon to 95.6 in the cultivars Merlot and Chardonnay. A higher percentage of eyes with 2 embryonic buds in the cultivars Merlot and Viorica who have this indicator amounted to, respectively: 75,6 and 70.6%. Forecasting a crop of grapes next year to trim the bushes on the proposed method will provide a full crop of grapes

The article presents the results of the study of yield, mechanical and biochemical composition of Merlot grape vine variety and its two clones in the conditions of Taman and Anapa subzone of the Krasnodar region. Clones differ from the maternal variety by the large number of berries in the bunch, the mass of the berry, the average mass of the bunch and the yield. Maternal variety and its clones are characterized by very close percentages of berries and combs in the bunch and by indicators of the structure, composition and structure of the bunch. By the yield of juice percentage, in different growth areas clones showed the same results as the original variety. According to the content of glucose, fructose, organic acids (wine, apple, citric, succinic), potassium, sodium, magnesium and calcium cations in the juice of berries, there is no reliable differences between the original variety and its clones. The content of dry substances, sugars, titrated acids, and the sugar-acid coefficient depended on the yield, but in general, they were favorable for the production of wine materials and juices. Merlo phanagoriiskii and Merloc are ecologically plastic and belong to the first group of varieties that retain their valuable characteristics in different areas of growth