Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Troshin Leonid Petrovich

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Kuban State Agrarian University

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Articles count: 170

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abstract 1271703070 issue 127 pp. 980 – 995 31.03.2017 ru 441
The article presents the results of the study of yield, mechanical and biochemical composition of Merlot grape vine variety and its two clones in the conditions of Taman and Anapa subzone of the Krasnodar region. Clones differ from the maternal variety by the large number of berries in the bunch, the mass of the berry, the average mass of the bunch and the yield. Maternal variety and its clones are characterized by very close percentages of berries and combs in the bunch and by indicators of the structure, composition and structure of the bunch. By the yield of juice percentage, in different growth areas clones showed the same results as the original variety. According to the content of glucose, fructose, organic acids (wine, apple, citric, succinic), potassium, sodium, magnesium and calcium cations in the juice of berries, there is no reliable differences between the original variety and its clones. The content of dry substances, sugars, titrated acids, and the sugar-acid coefficient depended on the yield, but in general, they were favorable for the production of wine materials and juices. Merlo phanagoriiskii and Merloc are ecologically plastic and belong to the first group of varieties that retain their valuable characteristics in different areas of growth
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abstract 1291705083 issue 129 pp. 1140 – 1157 31.05.2017 ru 727
The article presents results of studying the oenology and the biochemistry of the Cabernet-Sauvignon grapes in different areas of the Krasnodar region - Taman, Anapa, Abrau-Dyurso, Gelendzhik and Krasnodar. The yields, the mechanical composition of the grapes, the biochemical indicators of the juice of berries were compared. The cultivation zone affects the yield, the average mass of the bunch, the number and mass of berries in the bunch, the mass of the crest, but according to the percentage of berries and crest in the bunch, the bunch structure (ratio of mass of berries to mass of the ridge) does not significantly differ between growing zones. The mass of 100 berries and 100 seeds, the weight of seeds in 100 berries, the clustering index (the ratio of pulp with juice to peel) can differ in different places of growth, but with insignificant coefficients of variation. The composition of the bunch as a percentage of the total mass varies little over the growing zones: the ridges make up 5.6- 5.7%, the skin 4.9-5.2%, the seeds 4.8-4.9%, the pulp with the juice - 84.3-84.5%. Structural and berry indicators varied in the range of 5.4-5.5 and 76.84, respectively. Depending on the place of cultivation, the yield of the grape must in laboratory conditions was 75.1-75.7%. Analysis of the structure, composition and the structure of bunches showed that the mechanical composition of Cabernet-Sauvignon refers to the first group of varieties that retain their valuable characteristics when changing the growing conditions. When harvesting, the mass concentration of sugars was 19-19.8%, titrated acidity - 8.1-9.2 g / dm3, pH - 3.3- 3.4. Depending on the place of cultivation of the content of organic acids in the juice of berries was: wine - 4917-5918 mg / dm3, apple - 1926-2763, lemon - 215-416 mg / dm3. In different places of cultivation in% of the total amount of organic acids studied, the content of tartaric acid varied from 61.7 to 71.4%, apple - from 24.7 to 33.3%, lemon - from 3 to 5%. Thus, the content of organic acids in the juice of Cabernet-Sauvignon berries depends on the specific growing conditions. Depending on the growing places, the mass concentration of potassium cations is 2630-3508 mg / dm3, sodium 162-436, magnesium 171-230, calcium 185-255 mg / dm3. However, in most cases, as a percentage of the total cation content, there are no differences depending on the site of growth. Thus, the biochemical parameters of the juice of CabernetSauvignon berries depend on the specific natural conditions of the terrain and, according to these parameters, the variety belongs to the second group of varieties with less plasticity to different growing conditions
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abstract 1301706090 issue 130 pp. 1236 – 1248 30.06.2017 ru 314
The article reviews the results of studying the influence of mineral fertilizers on the qualitative indices of the Merlot grape variety in the Anapo-Taman zone of the Krasnodar region. The technology of cultivation of grapes on the experimental plot was in accordance with the accepted for the given zone and culture. Agrobiological work was carried out at the optimal time and had a high quality of execution. Grape bushes are laid in a 3.0 x 2.0 m pattern. Formation - a twoshoulder horizontal cordon. On the bushes we formed the same load of shoots and bunches. Scheme of the experiment: option 1 – no fertilizers (control); option 2-superphosphate with a mixture of potassium salt (P90K90) from the autumn; option 3 –nitroammofoska (N120P120K120) from autumn; option 4 – ammonium nitrate (N60) in early spring. Analysis of the results of studies on the study of biological features of growth, fruiting, yield and quality of Merlot grape varieties on the background of the use of mineral fertilizers shows their high efficiency in the conditions of the AnapoTamanskaya zone of the Krasnodar region and can be recommended for use in production conditions. Mineral fertilizers ensures the receipt of dry bulk wine of superior quality control sample (without fertilizers) the concentration of tartaric acid 17.3 25.1 percent, phenolic compounds 17.1 – 40.0 %, and alcohol content of 7.2 and 9.3 %, titratable acids 11.8 and 13.7 %, given extract by 17.4 %, with a decrease of the mass concentration of total sulfur dioxide and pH. The most effective is the introduction of NPK (N120P120K120)
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abstract 1311707103 issue 131 pp. 1248 – 1270 29.09.2017 ru 401
Vasily Nosulchak, the newest complex-resistant tableless seedless grape variety, was bred in Greece by Pantelei Zamanidi and Leonid Troshin in 2013 by crossing the Talisman variety with the Yanaky variety. The created variety is a complex inter-species Euro-American-Amur hybrid. The duration of the production period is 146-155 days. The growth of shoots is strong. The degree of grapevine maturing high. Yield is very high. Percentage of fruit-bearing shoots 90. Average weight of bunches 900 g. It is stainable for high winter hardiness, drought resistance and increased resistance to fungal diseases, tolerant to phylloxera. The top of the young shoot is green without pubescence. Young shoots are green, without pubescence. The flower is hermaphroditic. The cluster is large, conical, branching, winged, of medium density. The berry is medium-sized, short elliptical, green-yellow. Peel is thin, strong. The pulp is juicy, with a varietal flavor. The sugar content is high. Rudiments of seeds are soft. The variety is intended for fresh consumption and kishmish production. The table high-yielding seedless variety Vasiliy Nosulchak in terms of winter hardiness, resistance to diseases and pests significantly exceeds all Eurasian varieties of table and kishmish designation. Can be used as a table grapevine for fresh consumption on site and for export, as well as for the production of high-quality dried products. As a winter-hardy variety, it is very promising for cultivation in covered viticulture zones, where table varieties require shelter for the winter. It is of great interest for selection work in breeding frost-resistant, diseases and pests of seedless varieties. To determine the influence of different ecological conditions on the growth, development, quantity and quality of the crop, the variety must be tested on all continents in different ecological and geographical areas of cultivation - in the grapes producing countries of America, Eurasia, Australia, Africa
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abstract 1331709018 issue 133 pp. 196 – 205 30.11.2017 ru 442
The influence of growth, its ripened part and the diameter of the vine at the base of the shoot on the growth power of 922 seedlings of 55 combinations of crosses in 2011 and 2012 was studied (planting scheme 3 x 1 m), seedlings of grapes of the second and third years of vegetation in the field conditions of the Southern coast of Crimea. The mean vine maturation for all populations was 64.7%, the diameter at the base of the shoots was 3.3 mm. After pruning to reverse growth, in the third year of vegetation of seedlings (2014), the length of growth of shoots increased significantly and the average for populations it became 73.3 cm. The coefficient of variation increased, 26%, - it acquired the character of a significant deviation from the general population. This happened because the seedlings, which had a vine growth less than 25 cm in the second year of vegetation, had a growth of only 35 cm in the third year, whereas in all seedlings that had a growth of more than 30 cm, in the following year shoots increased almost 1, 5 and 2 times more. Formulas are proposed for estimating the growth force of seedlings according to the 9-score scale OIV (OIV)
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abstract 1341710026 issue 134 pp. 303 – 327 29.12.2017 ru 858
The article presents the structural and phylogenetic analysis of VIT_02s0033g00410, VIT_02s0033g00390 and VIT_02s0033g00450 genes of the grapevine genome and the closely related orthologous gene MYB114 of the Arabidopsis genome. These genes are responsible for the biosynthesis of anthocyanin in the organs of model plants and are of interest not only for practical production and breeding, but also for fundamental research. These genes were analyzed for GCcomposition of nucleotides, the presence of cisregulatory elements and promoter regions. DNA and protein sequences were aligned to look for similar elements, which allowed further analysis of the ultraconservative domains of four genes. Based on the results of search and identification of the conservative regions, a cluster tree was constructed, which made it possible to identify the separation of gene sidelines from, presumably, the main one. At the same time, the construction of consensus trees based on DNA and protein sequences revealed their absolute similarity. "The Minimal Evolution Tree" allowed calculating the approximate dates of the appearance of the mutations and the divergence times of the gene branches between each other. At the same time, the appearance of the Vitis genus and its separation from the Rosales was taken as the time first divergence point. In the end, homologous metabolic pathways were searched between grapevine and Arabidopsis, which revealed the presence of homologous proteins in the grape proteome. In this turn, it already confirms the existence of similar biosynthetic pathways and, as a consequence, interactions such as "DNA-protein" and "protein-protein"
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abstract 1351801012 issue 135 pp. 125 – 146 31.01.2018 ru 316
The generalized long-term experience on creation of base of Queen cells in grape varieties, improved in vitro culture, on the sandy array of Ust-Donetsk district of Rostov region. Problems frequently encountered on sandy soils and ways of their solution are shown. We focus on: rehabilitation of the landing of plants of grapes in the conditions of greenhouses, open ground, the optimum time and methods of planting. The work shows the effectiveness of different dressings and types of fertilizers, influence of drugs of new generation, affected the uterine protection of plantations from pests and disease, deep sandy soils freezing in winter, especially the development of root system of different varieties. On the basis of generalization of long-term data, we suggest the most appropriate solutions, and developed technology bookmarks and conducting basic queen cells from healthy in vitro planting material of grapes in the sandy array
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abstract 1401806018 issue 140 pp. 42 – 59 29.06.2018 ru 273
Intensive viticulture as a sub-branch of the grape-wine industry of agriculture is developing due to the introduction of modern scientific developments. The intensification of production consists of laying vineyards with planting material of higher biological categories, highly productive clones of traditional and long-established selection varieties; advanced agrotechnological techniques and mechanized operations, etc. For import substitution of table grapevine in our country, it is especially important to introduce new big-berry varieties from early to late maturity, providing a continuous 100-day conveyor for harvesting, with a bunch of grapes, various colors and the shape of berries. In many countries of the world with developed viticulture, breeding programs are being conducted to create competitive table varieties. As a result, there were obtained Muscat of Italy (Italy), Cardinal, Red Globe (USA), Moldova (Moldova), widely spread all over the world. But the world market does not stand still and breeders are working on new varieties. In recent years, there has been a trend of enlargement of berries from 8 to 24 grams, preference is given to varieties with pink and red berries. So, for example, created in Ukraine - a very early variety of Livia; in Russia - the variety clones of Livia K and Rochefort K, the early varieties of the Memory of the Teacher, the Gift of the Nesvetay, the Preobraghenie and the Jubilee of Novocherkassk, and others. These varieties are bred according to the developed model, which shows the genetic regularities of the formation of maximum expression in transgressive recombinants in quantitative (mass of berries and bunches) and qualitative (shape and color of berries) traits associated with maturation
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abstract 1461902011 issue 146 pp. 38 – 48 28.02.2019 ru 291
The analysis of varietal and age structure of vineyards occupied by 29 varieties and clones of the technical direction of use in the "Alushta" branch of FSUE "PJSC "Massandra". Taking into account the ecological and climatic conditions of the Alushta valley and the specialization of the economy, the largest share in the assortment of technical varieties is occupied by red grape varieties: Cabernet Sauvignon and clones of Cabernet Sauvignon R5, R8, VCR8, No. 169 including (26.68%), Bastardo Magarachsky and Bastardo clone VCR1 (12.06%), Saperavi (7.33%), Krasnostop zolotovsky (5.69%), Merlot and clone Merlot R3 (4.62 %). White varieties are in a smaller percentage: white Muscat and white Muscat clone R3 (8.47 %), white Kokur (7.1%), Aligote (3.82%), Semillon and clones Semillon № 173 and № 299 (3.68%), Gars level (2.99%). Plantings in the most productive age of 6-10 and 11-15 years are 57.5 % of the total area. At the same time, the share of introduced clones of varieties accounts for 26.65 %. In general, the age structure of the vineyards is favorable. The company produces high quality wine materials for production of traditional Federal state unitary enterprise "PJSC "Massandra" wines: white Port Alushta, Alushta red Port, pink Port Alushta, Alushta red Table, Pinot Gris, Massandra, Madera Crimean Alushta Tokay, white Muscat, Massandra Kokur, Semillon Alushta and sherry wine. In addition, there are new brands of semi-sweet white and red wines Pearl Massandra, as well as varietal wine materials Aligote, Cabernet, Sauvignon, Merlot, Saperavi, Chardonnay, Bastardo and Semillon. Analysis of the assortment shows that in the production plantings of "Alushta" branch of FSUE "PJSC "Massandra" there are varieties of medium and late ripening, which does not create peaks during harvesting and processing, ensuring uniform flow of raw materials for winemaking. It is necessary to expand the assortment of the economy due to a number of promising varieties of Syrah, Petit Verdot, Malbec, Sangiovese and others with a complex of economically valuable properties introduced from other regions
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abstract 1471903014 issue 147 pp. 53 – 61 29.03.2019 ru 264
The article presents the results of the fertilizers use effect on dry wine materials produced from the Chardonnay variety, grown in the Anapo-Taman soil and climatic zone of the Krasnodar region, Russia. As a result of wine materials analysis, increase of tartaric acid was noted, with the autumn application of phosphorus-potassium fertilizers and the spring application of ammonium nitrate. At the same time, its optimum content was noted with nitroammophos. Also, the minimum concentration of malic acids was detected by the application of nitroammophos with potassium fertilizers. In its turn, early spring application of ammonium nitrate reduced the concentration of malic acid below the control sample (without fertilizers). It was revealed that the use of mineral fertilizers in this winemaking zone also contributed to a reduction of lactic acid in the must. At the same time, the application of nitroammophos (especially together with other mineral fertilizers), contributed to the accumulation of citric and succinic acids, as well as phenolic compounds. Moreover, the application of phosphate-potassium fertilizers in the autumn contributed to the accumulation, and, consequently, also to the increase in the content of phenolic compounds in the resulting must. In addition, the studied samples of wine materials contained high alcohol concentrations and low concentrations of sugars with volatile acids. At the same time, the maximum amount of alcohol exceeding its content in the control sample was revealed when phosphorus-potassium fertilizers were used together with nitroammophos fertilizers. It is also noted that the application of ammonium nitrate did not affect this indicator. As a result, the use of mineral fertilizers in the studied concentrations made it possible to prepare wine materials that contained a low concentration of sulfur dioxide, which is substantially lower than the harmful concentration for humans. In addition, increased concentrations of hydrogen ions in variants with the application of phosphate-potassium fertilizers have made it possible to reduce the number of iron tanates and the intensity of oxidation-reduction processes