Scientific Journal of KubSAU

The article presents results of the study of yield, mechanical composition and quality of bunches of newest white grapevine varieties called Artemis, Sauvignon tamanskii and Inkroche tamanskii, in the conditions of Taman in the Krasnodar region. The yield of the studied genotypes ranged from 10.4 to 13.3 t / ha: the highest yield was in Artemis, and the lowest – in Sauvignon tamanskii. The average weight of the bunch was in Sauvignon tamanskii (152 g), the variety Artemis had 143 g and Inkroche tamanskii had 130 g of bunch weight. The percentage of berries in clusters ranged from 95.3 in Artemis to 95.9 in Inkroche tamanskii and Sauvignon tamanskii. The ratio of fruit mass to the mass of crests was 20.3 in the varieties of Artemis and 23.5 in Inkroche tamanskii and Sauvignon tamanskii. In genotypes of Artemis, Sauvignon and Inkroche Taman Taman the weight of 100 berries was 200, 160 and 130 g, respectively, the mass of 100 seeds - 4.6; 3.1 and 2.9 g, the average weight of the pulp from the juice to the weight of the skin - 24.3; 19.5; 15.9. The percentage of pulp and juice in clusters of the studied varieties was 85,9-89,1%, and the ratio of pulp to juice to the solid residue 6,1-8,2. Yield of musts from the studied sample was 74,5-75,4%. The content of sugars in the berries (17,5-19,6 g / 100 cm3) was dependent on the yield, the characteristics of varieties and timing of the harvest, but the harvest was favorable for the production of beverages and wine materials

The article presents the data on the formation of the embryonic fruitfulness of central ovaries of wintering buds of the group of technical grape varieties with white berries - White Muscat; Pinot White, Chardonnay, Citron Magaracha, Pervenets Magaracha; Riesling, Viorica, Riton, Crystal in the conditions of Anapa-Taman zone. There were revealed the rates of embryonic fruitfulness of central ovaries of buds of studied cultivars and fruiting indices of vegetative shoots developed from them. In all studied grape varieties there was revealed a high percentage of fruitful buds from 84,1 in the variety of Riton at 97.2 at Viorica; and the percentage of fertile vegetative shoots from 81,8 at the variety Citron Magaracha to 97.2 in the variety White Muscat. At the leveled load of bushes, vegetative shoots and the same scheme of planting of bushes (3 x 2 m), the highest yield in terms per hectare showed the varieties Pervenets Magaracha, Viorica, Riton, Crystal, Riesling and Citron Magarach. When assessing the economic efficiency the highest net income and level of profitability were identified in the varieties of Citron Magaracha, Pervenets Magaracha, Viorica and Riton. In order to determine which buds will give us shoots with large, well-developed (well-differentiated) buds, and which will not give (weakly differentiated), it is necessary "to look inside a bud". But even already formed germs of inflorescences in the bud are able in a few days in spring or dedifferentiate or degrade depending on the influence of external conditions. Scientists have learned to use this ability to increase the maximum possible yield in years of severe damage of grape by frosts. Firstly, in frosty winters the central buds wither out. The replacing buds usually have poor fruiting and bad productivity during years. In such cases, it is more profitable to conduct a small cutting of angle buds arranged in a circle at the base of a shoot. At the beginning of the second vegetation phase, these buds will obtain the great bulk plastic substances, which will cause the dedifferentiation of rudiments of inflorescences in them. Thus, it is possible not only to restore rapidly the normal shape of a bush, but to obtain a good harvest this year

Vasily Nosulchak, the newest complex-resistant tableless seedless grape variety, was bred in Greece by Pantelei Zamanidi and Leonid Troshin in 2013 by crossing the Talisman variety with the Yanaky variety. The created variety is a complex inter-species Euro-American-Amur hybrid. The duration of the production period is 146-155 days. The growth of shoots is strong. The degree of grapevine maturing high. Yield is very high. Percentage of fruit-bearing shoots 90. Average weight of bunches 900 g. It is stainable for high winter hardiness, drought resistance and increased resistance to fungal diseases, tolerant to phylloxera. The top of the young shoot is green without pubescence. Young shoots are green, without pubescence. The flower is hermaphroditic. The cluster is large, conical, branching, winged, of medium density. The berry is medium-sized, short elliptical, green-yellow. Peel is thin, strong. The pulp is juicy, with a varietal flavor. The sugar content is high. Rudiments of seeds are soft. The variety is intended for fresh consumption and kishmish production. The table high-yielding seedless variety Vasiliy Nosulchak in terms of winter hardiness, resistance to diseases and pests significantly exceeds all Eurasian varieties of table and kishmish designation. Can be used as a table grapevine for fresh consumption on site and for export, as well as for the production of high-quality dried products. As a winter-hardy variety, it is very promising for cultivation in covered viticulture zones, where table varieties require shelter for the winter. It is of great interest for selection work in breeding frost-resistant, diseases and pests of seedless varieties. To determine the influence of different ecological conditions on the growth, development, quantity and quality of the crop, the variety must be tested on all continents in different ecological and geographical areas of cultivation - in the grapes producing countries of America, Eurasia, Australia, Africa

The influence of growth, its ripened part and the diameter of the vine at the base of the shoot on the growth power of 922 seedlings of 55 combinations of crosses in 2011 and 2012 was studied (planting scheme 3 x 1 m), seedlings of grapes of the second and third years of vegetation in the field conditions of the Southern coast of Crimea. The mean vine maturation for all populations was 64.7%, the diameter at the base of the shoots was 3.3 mm. After pruning to reverse growth, in the third year of vegetation of seedlings (2014), the length of growth of shoots increased significantly and the average for populations it became 73.3 cm. The coefficient of variation increased, 26%, - it acquired the character of a significant deviation from the general population. This happened because the seedlings, which had a vine growth less than 25 cm in the second year of vegetation, had a growth of only 35 cm in the third year, whereas in all seedlings that had a growth of more than 30 cm, in the following year shoots increased almost 1, 5 and 2 times more. Formulas are proposed for estimating the growth force of seedlings according to the 9-score scale OIV (OIV)

Clonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way

Numerous studies have found a different quality of the buds along the length of the fruit arms, which expressed various degrees establishing embryonic inflorescences in the central buds. It is revealed that many of the grapes have more fruit-bearing eyes formed on average (4-6 knots) and upper (7 to 10 knots) levels of fruit escape, compared to the lower (1-3 nodes) layer. In connection with the different quality of the buds along the length of fruit vines should be used and different ways of cutting them. It is necessary to consider the biological characteristics of varieties, ways of doing and shaping the bushes, the weather conditions of the area during the growing season and relative calm. To receive annually a stable high yield of grapes, we must first set the optimal length of pruning fruit shoots and optimal load on the Bush healthy eyes. This requires for each class on the eve of trimming bushes to carry out the analysis of the wintering holes along the length of the fruit shoots and to identify the embryonic fruitfulness of Central buds, i.e. to carry out the prediction of grape yield next year. The article presents data on the formation of the embryonic fruitfulness of Central buds of wintering buds at the Taman group of grapes – Arcadia, Viorica, Hibernal, Cabernet Sauvignon, Merlot, Moldova, Pinot white, Riesling, Saperavi, Traminer pink, Tsimlyansky black, Chardonnay. Found higher rates of embryonic fruitfulness of Central buds buds in the studied grape varieties - Chardonnay, Riesling, Viorica, Hibernal, Merlot, Moldova, where the coefficients of fruiting ranged from 1.40 m from Riesling to 1.69 from Merlot; factor productivity of 1.58 from the Chardonnay to 1.77 from Merlo. The high percentage of fruitful buds from 80,8 from Cabernet Sauvignon to 95.6 in the cultivars Merlot and Chardonnay. A higher percentage of eyes with 2 embryonic buds in the cultivars Merlot and Viorica who have this indicator amounted to, respectively: 75,6 and 70.6%. Forecasting a crop of grapes next year to trim the bushes on the proposed method will provide a full crop of grapes

The article presents the results of the study of yield, mechanical and biochemical composition of Merlot grape vine variety and its two clones in the conditions of Taman and Anapa subzone of the Krasnodar region. Clones differ from the maternal variety by the large number of berries in the bunch, the mass of the berry, the average mass of the bunch and the yield. Maternal variety and its clones are characterized by very close percentages of berries and combs in the bunch and by indicators of the structure, composition and structure of the bunch. By the yield of juice percentage, in different growth areas clones showed the same results as the original variety. According to the content of glucose, fructose, organic acids (wine, apple, citric, succinic), potassium, sodium, magnesium and calcium cations in the juice of berries, there is no reliable differences between the original variety and its clones. The content of dry substances, sugars, titrated acids, and the sugar-acid coefficient depended on the yield, but in general, they were favorable for the production of wine materials and juices. Merlo phanagoriiskii and Merloc are ecologically plastic and belong to the first group of varieties that retain their valuable characteristics in different areas of growth

In this article we present information on features of microcloning in culture of in vitro of perspective for Eurasia, pink and white-berry table genetic grades of grapes by I. A. Kostrikin, V. N. Kraynov and V. V. Zagorulko. Researches were conducted at an input stage in culture of in vitro of meristems of 0,1-0,2 mm in size. At the majority of grades and forms, we noted good regeneration abilities in the conditions of sterile culture. It is bound to the fact that almost all the studied grades: Anyuta, Bogatyanovsky, the Princess Olga, Preobrazhenie, Anniversary of Novocherkassk, Favor are received from crossing of couple the Mascot × Sultana-ray grape. And only grades called Libya and Lowland which showed lower regeneration properties at stages of proliferation and rooting of shoots are received from crossing the couple of Flamingo × Arkadya and (Talisman x Tomaysky) respectively. At the same time, the grade of Talisman, being one of the parents of the majority of the studied grades, showed stable results at all stages of cultivation

It is well known that genetics studies the mechanisms of variation/heredity and widely uses the concept of "genetic information". While genetics considers the information as the content of the genetic code - structure of DNA and RNA included in the cell of a living organism. Genetics examines the mechanisms of recording, copying, readout of genetic information, the possibility of its modification and its influence on the characteristics and properties of the organism. In conversational and scientific language we know phrases, such as "Genes contain information about the characteristics/properties of the body." Paradoxically, we see no attempts to determine the amount of information contained in specific genes on specific characteristics or phenotypic properties of the organism. It would seem that the application of information theory in genetics is a completely natural and suggests itself. More strange that there are practically no works devoted to the application of information theory for solving problems of genetics. This article is intended, to some extent, to fill this gap on the example of calculating the amount of information in the genes of the characteristics or properties of different grape varieties. It examines the application of automated system-cognitive analysis (ASC-analysis), its mathematical model – system of information theory and software tools – intellectual system called "Eidos" for solving one of the important tasks of genetics: determine the amount of information contained in the genes on various phenotypic characteristics/properties of the grapes. To solve this problem, we perform the following steps: 1) cognitive-targeted structuring of the subject area; 2) the formalization of the subject area, i.e. development of classification and descriptive dials and graduations and training samples; 3) synthesis and verification of information model, reflecting the amount of information in the genes on the phenotypic characteristics/properties (multiparameter typing); 4) displaying the information about the genetic determination system of phenotypic characteristics/properties (SWOT analysis of Fennovoima); 5) displaying the information about the strength and direction of influence of a specific gene on phenotypic characteristics/properties (SWOT-diagrams of genes); 6) the solution to the problem of system identification phenotypic characteristics/properties by the presence of certain genes; 7) quantification of the similarities-differences of the various phenotypic characteristics/properties, upon determination system genes. A specific phenotypic property (or characteristic) is regarded as a noisy genetic text, including genetic information about the true gene property (clean signal) and the noise that distorts this information due to the random effects of the environment. The software tool of the ask-analysis which is "Eidos" intellectual system provides the noise suppression and the selection of true signal

The article presents the results of studies of 92 indigenous varieties and 15 wild genotypes by 24 microsatellite markers. As a result a locus database about germplasm in the collection stored at the Russian ampelographical collection AZOSViV (Anapa Zone Experimental Station of Viticulture and winemaking) was created. During the research of varietal breeding populations confirmed the validity in the production of the cultivar of Krasnostop anapsky with obvious changes in microsatellite sequences as a clone of famous indigenous Don cultivar Krasnostop Zolotovskii. This clone was selected in 1953 by prominent agrotechnologist Zotkin Ivan Ivanovich which is propagated by the author and his followers, and after a formal registration submitted to state testing in 2007 in GSK RF, zoned in the North Caucasus region (number 6) in 2014 year. Sort -clone Krasnostop anapsky only in the Kuban today cultivating in 35 hectares. By using molecular genetic techniques, now clonal selection of vine grapes is reasonably improving. Recent data collected by us in the work process, were analyzed a comprehensive manner, making it possible to draw conclusions about distinctness of grape varieties. Some clones were studied by DNA primers, based on retrotransposon sequences