Scientific Journal of KubSAU

This article presents information about the features of biotechnology as the driving force of scientific and technological progress. The national programs of the leading countries of the world, it is one of the priority sectors, reflecting the level of the socio-economic condition of the society. Biotechnology is now successfully solves such vital tasks as providing food, the establishment of effective medicaments, obtaining fuel based on renewable raw materials, maintaining ecological balance, conservation of biological resources of the Earth. The development of agriculture in modern conditions is impossible without agricultural biotechnology. It is directly related to viticulture. Choosing an object of an integrated system (embryos, apical meristem, axillary buds), it is possible to clone plants, i.e. produce plants identical to the original. If the same as the object to use isolated cells or protoplasts, in this case, there will most likely altered versions, creating diversity for the breeder. Genetic engineering – the science of younger, since the establishment of the first chimeric DNA molecule. The origin of genetic engineering is rooted in the development of molecular genetics, biochemistry. These technologies, undoubtedly progressive, but their biological safety is still insufficiently explored and is a danger to all life on Earth. The leading Western powers carried out strict control over the introduction of transgenic crop plants, as they are in agrocenosis new biological risks that may adversely affect the plants, animals and humans. In Russia, as in other countries, have already adopted the law “State regulation of genetic engineering”

On the example of economic analysis, we have characterized the life cycle of a scientific institution, the history of the formation and development of which covers almost a three-hundred-year period. The article determines features of the methodology for identifying the stages of the life cycle of a scientific organization and identifying factors that determine the stage of the life cycle

Step by step results of many year researches of clonal selection of grape varieties: Clairette white, Saperavi, Sauvignon white and Chardonnay, growing in the Ltd company “ Pobeda” of Temryk district of Krasnodar region are cited in the article. Highly productive proto clones were selected. Clairette white is the best one as a variety from them and it was given to the state experiment under the name Clairette temryk.

The clone selection has very outstanding position behind the actual problems of modern viticulture in the world and in our country. This problem usually has the primary importance behind the scientist who works with grape in all the countries such as USA, German, French, Spain, Italy, Hungary, Bulgaria, Moldavia, Ukraine and etc. In our country last years some positions becomes solving in the plane of scientific research work. Consequently, in the article the history and the situation of clone selection of grape on the Eurasian continent is widely covered.

The article gives the results of the study of the texture of the grapes and biochemical characteristics of red wine grape varieties of Cabernet Sauvignon, Cabernet Franc, Merlot, Sapeari, Tsimladar, Pinot Noir for the production of juice of direct extraction and blending. The studied varieties in terms of the structure of the cluster may be divided into three groups of blackpinene (21.1), Cabernet Franc and Tsimladar (18.7 - 17.5), Cabernet Sauvignon, Merlot, Saperavi (16.5 - 16.8) , and bunches of addition in terms of two groups - Tsimladar (17.6), Cabernet - Sauvignon and other (15.6 - 16.4). The structural component of grapes varieties ranged from 5.2 to 5.9, berry index - from 62.1 to 83.4, the output of the wort from 74.2 to 76.3%. The content of sugars and acids in the berries of all varieties is favorable for the production of beverages. The largest Tartaric acid content was found in the juice of the varieties of Cabernet Sauvignon, Pinot Black, malic acid - in the juice of varieties Saperavi, Merlot, Tsimladar, citric acid - in the juice Tsimladar varieties, Cabernet Sauvignon, Saperavi. The ratio of tartaric acid to the content of the apple juice Cabernet Sauvignon was 2.9: 1, Cabernet Franc 2.6: 1, Pinot Black 2.5: 1, Merlot and Tsimladar 1.6: 1, 1.1 Sapevari: 1. According to the content of potassium cations, sodium, magnesium and calcium there were chosen three varieties of Cabernet Sauvignon and Cabernet Franc. The lowest content of potassium and magnesium cations was in the variety of Tsimladar, Sodium – in the varieties Merlot and Saperavi, calcium – in Saperavi. Excellent tasting commended for juices was given to Cabernet Sauvignon, Saperavi, Tsimladar, good – to Cabernet Franc, Merlot, Pinot black. Best blending juice obtained by mixing the juice of the grape of the varieties of Cabernet Sauvignon and Pinot black 70:30, Saperavi and Pinot black 50:50. From the grapes of produced varieties, we can produce juice and varietal by name ampelographic variety and blended to extend the range

The article presents the characteristics of the chemical composition of a complex of seven-resistant varieties, introduced in the Central zone of the Krasnodar region, and the quality produced from these natural clarified pasteurized juices, against three of the Eurasian, the control group. We recommend the most valuable varieties for production of high quality juices

The article contains the results of the study of mechanical composition of grapes and biochemical characteristics of white wine grapes for the production of juice of direct extraction and blending. Thus, the yield of studied grapes ranged from 7.5 to 13.2 t / ha: highest yielding varieties and hybrids of Pervenets Magaracha and Tsitronnyy Magaracha, and the lowest - Chardonnay and Pinot blanc. At an average weight of clusters of varieties were divided into three groups - large clusters (230-243 g) in varieties Pervenets Magaracha and Tsitronnyy Magaracha, medium (181-184 g) - the varieties Chardonnay and Pinot blanc, small (112-116 g) - the varieties Bianca and Viorica. By weight of the 100 berries were divided into four groups - Bianca and Viorica (I), Pervenets Magaracha (II), Chardonnay (III), Pinot blanc and Tsitronnyy Magaracha (IV). Weight of 100 seeds from the largest varieties Pinot blanc and Bianca, and the lowest - in the varieties of Tsitronnyy Magaracha and Chardonnay. The solids content in the juices of these varieties ranged from 19.4 to 21.8%, which corresponded to branded products and higher of commodity varieties. Mass concentration of sugars ranged from 18.4 to 21.3 g / 100 cm3. Titratable acid content in the juice was varied from 0.65 to 0.78 g / 100 cm3. Sugar-acid ratio, defined as the ratio of concentration of sugars and titratable acids ranged from 23.6 to 30. The recommended values for this indicator were within 22-30. According to the results of the tasting, we rated "excellent" the juices from the varieties of Tsitronnyy Magaracha, Viorica (19 points) and Bianca (17 points); "good" marks were given to the juices from Pinot blanc, Chardonnay, Pervenets Magaracha: this means that we can get juices of excellent quality from the grape varieties under study with the title of ampelographic variety; in addition, the juice of the grape of varieties Chardonnay, Pinot blanc, Bianca, Pervenets Magaracha due to their potassium content will be functional to improve the cardiovascular system. On the basis of the tasting we have conducted mixing of the juices from the varieties of Viorica and citron Magaracha with other juice varieties 50:50 and 70:30. Sensory evaluation showed that blending juices from the varieties of Viorika and citron juice Tsitronnyy Magaracha other varieties improves product quality and 50:50 increases by one the total score, and 70:30 - two units. Blend of the varieties of Viorica and Tsitronnyy Magaracha does not increase the total score, but gives the product a special flavor and aroma. Hence, using the grape varieties under study we can produce varietal juices with the name of their ampelographic type and blended juices to extend the assortment

The article presents the characteristics of the mechanical composition of clusters, the chemical composition of grape juice and fresh grapes and wine tasting evaluation of three varieties of table. We have identified the following uvologic indicators of clusters of cultivars Flora, Nisina and Anyuta: structure - 90.0; 91.5; 87.3; addition - 8.0; 6.6; 10.8; structural - 6.4; 5.4; 8.1; berries - 17.6; 15.0; 10.4. In the studied berries of grape varieties the mass concentrations of sugars and titratable acids were in the range 18,1-23,5 and 0,73-0,78 g/100 cm3 respectively. The combination of sweetness and acidity causes high taste properties of grapes. In grape berries of Flora and Nizina the concentration of fructose was two times more than glucose. Differences in the content wine, malic, lemon and succinic acids in berries varieties of Flora and Nizina were insignificant. In the berries of Anyuta variety, the concentration of lemon and succinic acids was more, malic was less than other varieties. Most of the studied varieties of berries contain malic and wine acids. More of cations of potassium, sodium, calcium and magnesium then in other varieties and it contain less than Anyuta berries. The difference in the content of various cations in the berries of varieties of Flora and Nizina is negligible. Tasting score of fresh grape of Flora variety was 7.0, Nisina - 7.8 and Aniyta - 7.6 points

Progress of breeding for resistance to diseases, pests and low temperatures is provided by cross-species hybridization. Institute of Vine and Wine "Magarach" is one of the leading centers of selection of grapes in the world. The breeding program of deducing grapes at the institute is based on the study and attracting the world's gene pool, including and industrial of the Russian Federation assortment of grapes. Analysis assortment shows that the share of productive, highquality varieties with group resistance to diseases, pests and extreme environmental factors is very small. The main method of selection of grapes of a new generation who have genetically determined traits earliness, resistance to biotic and abiotic factors is crossing complex interspecific hybrids and forms of their involvement with each other and with the forms of species Vitis vinifera, belonging to different ecogeographical groups, saturating crossing between hybrid forms with complex features, the appropriate selection of reference. For vigorous offspring is most effective as a parent to use a local variety of forms of Crohn and father - interspecific cultivar Alminsky

The Mavrostifo – Greece aboriginal variety. It is belong to the group of varieties convar. pontica subconvar. balcanica Negr. The time of production period is 156-165 days. The variety of medium height. The degree of ripening of vine is higher. The yield is higher. The average mass of bunch is 360 g. It has higher drought-resistance and middle steadiness for mushrooms diseases. The leave is large, black-green, the middle dissection, five lobes (the lobes are recovered), from bottom side with a lot of hairs. There are two blossoms on the 3 and 4 nodes. The flower is hemofroditus. The bunch is low or big, long, conical-winged, density. The berry is average, round, black-blue. The pulp is juicy, with tasty variety after taste. There are two-three seeds in berry. The sugariness is higher. The variety is useful for preparing higher quality intensive colored red wines of different categories from southern zones of viticulture with warm and dry climate.