Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Troshin Leonid Petrovich

Scholastic degree


Academic rank

professor

Honorary rank

Organization, job position

Kuban State Agrarian University
   

Web site url

Email

lptroshin@mail.ru


Articles count: 169

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563 kb

EXTRACTING DNA WITH USING PEQGOLD PLANT DNA MINI KIT

abstract 0901306011 issue 90 pp. 167 – 176 30.06.2013 ru 1356
In the present article, the new method of DNA extraction from fresh and herbarium leafs of grape to their subsequent sequencing was described
355 kb

EXTRACTION OF DNA FROM LEAVES OF VITIS VINIFERA L.

abstract 0601006036 issue 60 pp. 571 – 587 29.06.2010 ru 1958
Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
409 kb

FEATURES OF DEVELOPMENT OF THE GENERATIVE ORGANS OF PLANTS OF GRAPES OF DIFFERENT ORIGIN IN THE CONDITIONS OF TAMAN

abstract 0971403010 issue 97 pp. 120 – 137 31.03.2014 ru 1182
The article presents data on the formation of the embryonic fruitfulness of central buds of wintering buds grapes of different origins in the period of relative calm of grapes and early spring period in dynamics with presence of positive air and soil temperatures
584 kb

FORECASTING OF GRAPE YIELD AND THE ESTABLISHMENT OF OPTIMUM BUSH LOADING DURING THE CUTTING IN BUDS ON THE PROPOSED YIELD ON THE EXAMPLE OF OAO AF «SOUTH»

abstract 1161602026 issue 116 pp. 352 – 368 29.02.2016 ru 713
The article presents the material of forecasting for grape yield of next year and establishing the optimal loading if cutting of bushes. The material includes 14 varieties of grapes, 11 of them are technical and 3 are table ones. For each year of stable high yield of grapes, it is necessary to pre-set the optimum length of fruit cutting of shoots and optimum load on the bush healthy eyes. To do this for each variety on the eve of trimming bushes we perform optimum productivity analysis of wintering buds of fruit along the length of shoots, i.e. we implement forecasting of grape yield for next year. We have a plan of forecasting for yields of vineyards by microscopy of wintering buds on one-year shoots of fruit ripened grapes in order to establish the potential of embryonic establishment of inflorescences in the central holes of buds. Based on the analysis of buds, the indices were calculated for wintering fruiting buds and their degree of damage during the growing season. It was revealed, that the majority of grape varieties under study shows high tab embryonic inflorescences in central buds in overwintering buds for next year yield. Higher rates at a rate of fruiting buds were wintering in the varieties: Moldova (section 27). - 1.66; Bianca (section 6). - 1.83; Kunlean (section 15). - 1.71; Merlot (section 14). - 1.64; Saperavi (section 56). - 1.76. The lowest rates of fructification - the varieties Muscat Hamburg (section 21) and Augustine (section 11) and were respectively 1.20 and 1.24. As a planned productivity, we offered the optimal loading model of cutting bushes buds. As a result of productivity analyzes of buds along the length of the fruit shoots in 2016 we recommended to carry out pruning of fruit annual shoots 3-4 buds of the form of AZOS-1 and the form of cordon - 5-6 buds
352 kb

FROST RESISTANCE OF CRIMEAN INDIGENOUS GRAPE VARIETIES AND THEIR HYBRIDS

abstract 1171603044 issue 117 pp. 684 – 697 31.03.2016 ru 499
The article highlights the issues of grape plants resistance to low temperatures; it is an urgent problem for all wine-growing regions of the world, located in the zone of risky agriculture. Losses caused by frost, indicate long-standing necessity of perfection of assortment in terms of enhancing its hardiness by enriching more resistant genotypes obtained both through the introduction or naturalization, as well as from the crossing of indigenous with interspecies hybrids. Methodology for evaluating frost resistance properties of the material served as a preparation for the originality of his research and experimental evaluation of the unique divisibility properties of the aim. Thus, we initially conducted hardening of matured cuttings at positive temperature plus 8 plus 4 ° C for 14 days (I quenching phase) and a negative temperature minus 5 ° C-minus 7 ° C for 9 days and minus 10 ° C-1 day (II hardening phase). Then the cuttings we frozen in the temperature range from minus 16 ° C to the next steps of consistent freezing (minus 16 ° C - 2 days; minus 18 ° C - 2 days; minus 20 ° C - 2 days; minus 22 ° C – 1day, minus 24 ° C - 1 day, minus 26 ° C - 1 day, minus 28 ° C - 1 day and minus 30 ° C - 8 hours). After freezing of cuttings of each variety, the sample was placed on the 3 days in the refrigerator at the temperature of + 2 ° C to their gradual thawing. Cuttings were grown at room temperature on water. As a result, obtained by the accounting: the lowest frost-resistance among the studied forms of parental Crimean origins was found in grades of Shabash, Soldayya and Solnechnodolinsky; the varieties of Dzevat kara, Kokur bely, Kokur cherny, Misket, Misgyuli kara, Sari Pandas were set as a weak level (-20 °C); Aybatly, Kefesiya, Kok pandas and Ekim kara have average level up to -22 °C; the highest frost-resistance - 24 °C – was shown by Khersonessky and Kapselsky. Also, we have identified synthetic frost-resistant F1-population and plus-transgressive selection form
3281 kb

GENE POOL OF INDIGENOUS GRAPE VARIETIES AND INTRODUCENTS IN ABKHAZIA

abstract 1001406009 issue 100 pp. 177 – 208 30.06.2014 ru 1504
This article provides an overview of results of the conducting surveys on screening and saving gene pool of indigenous grape varieties in the Republic of Abkhazia, which contributed to detection and identification of the 15 previously described in the literature of Abkhazian native grape varieties
516 kb

GENOTYPING OF GRAPE VARIETIES WITH USING MICROSATELLITE SSr MARKERS

abstract 0961402005 issue 96 pp. 53 – 65 28.02.2014 ru 1223
This article presents the results of genotyping of new perspective table and technical varieties and grapes protoclones. It was established that varieties of Citrine, Helios, Arcadia pink and Preobragenie show differ genetic diversity in four loci
4445 kb

GENOTYPING OF NEW PERSPECTIVE WINE GRAPES WITH USING MICROSATELLITE MARKERS

abstract 0981404010 issue 98 pp. 137 – 155 30.04.2014 ru 1771
In this article we have described our work on genotyping of new ten productive wine grape protoclones
695 kb

GENOTYPING OF THREE NEW PRODUCTIVE WINE VARIETIES WITH USING MICROSATELLITE MARKERS

abstract 0981404077 issue 98 pp. 1073 – 1087 30.04.2014 ru 1722
In this article we have described a study of genotyping of new three productive wine grapes protoclones: Rhine Rieslin, Verdot black and Johanniter
536 kb

GRAPE SORTIMENT OF TWO KUBAN ZONES

abstract 0370803009 issue 37 pp. 96 – 104 31.03.2008 ru 4066
Results of four-year (2004-2007) researches of perspective technical grape varieties by contrast zones of Krasnodar region : in central- Antaris, Beisug, Cabernek, Clairette Temryk, Litdar, Merlok, Muscat Kuban, Rislinalk, Risling Kuban, Shardonek, in Anapa-Krasnostop AZOS, Rubin AZOS and to the memory of Zotkina, were cited in the article. It was shown their superiority under control varieties. Ampelographical screening of gene pool was carried out in subzone Temryk on the plot of I.G. Karas’ in 2007 and in the result of which there were selected some perspective varieties: Arcadia, Victoria, Hybrid 342, Long-waited , Lora, Original.
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