Name
Troshin Leonid Petrovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
Articles count: 170
In the present article, the new method of DNA extraction from fresh and herbarium leafs of grape to their subsequent sequencing was described
Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
The article presents data on the formation of the embryonic fruitfulness of central buds of wintering buds grapes of different origins in the period of relative calm of grapes and early spring period in dynamics with presence of positive air and soil temperatures
The article presents the material of forecasting for grape
yield of next year and establishing the optimal loading if
cutting of bushes. The material includes 14 varieties of
grapes, 11 of them are technical and 3 are table ones. For
each year of stable high yield of grapes, it is necessary to
pre-set the optimum length of fruit cutting of shoots and
optimum load on the bush healthy eyes. To do this for
each variety on the eve of trimming bushes we perform
optimum productivity analysis of wintering buds of fruit
along the length of shoots, i.e. we implement forecasting
of grape yield for next year. We have a plan of forecasting
for yields of vineyards by microscopy of wintering
buds on one-year shoots of fruit ripened grapes in order
to establish the potential of embryonic establishment of
inflorescences in the central holes of buds. Based on the
analysis of buds, the indices were calculated for wintering
fruiting buds and their degree of damage during the
growing season. It was revealed, that the majority of
grape varieties under study shows high tab embryonic
inflorescences in central buds in overwintering buds for
next year yield. Higher rates at a rate of fruiting buds
were wintering in the varieties: Moldova (section 27). -
1.66; Bianca (section 6). - 1.83; Kunlean (section 15). -
1.71; Merlot (section 14). - 1.64; Saperavi (section 56). -
1.76. The lowest rates of fructification - the varieties
Muscat Hamburg (section 21) and Augustine (section
11) and were respectively 1.20 and 1.24. As a planned
productivity, we offered the optimal loading model of
cutting bushes buds. As a result of productivity analyzes
of buds along the length of the fruit shoots in 2016 we
recommended to carry out pruning of fruit annual shoots
3-4 buds of the form of AZOS-1 and the form of cordon
- 5-6 buds
The article highlights the issues of grape plants resistance
to low temperatures; it is an urgent problem for
all wine-growing regions of the world, located in the
zone of risky agriculture. Losses caused by frost, indicate
long-standing necessity of perfection of assortment
in terms of enhancing its hardiness by enriching
more resistant genotypes obtained both through the
introduction or naturalization, as well as from the
crossing of indigenous with interspecies hybrids. Methodology for evaluating frost resistance properties
of the material served as a preparation for the originality
of his research and experimental evaluation of the
unique divisibility properties of the aim. Thus, we initially
conducted hardening of matured cuttings at positive
temperature plus 8 plus 4 ° C for 14 days (I
quenching phase) and a negative temperature minus 5 °
C-minus 7 ° C for 9 days and minus 10 ° C-1 day (II
hardening phase). Then the cuttings we frozen in the
temperature range from minus 16 ° C to the next steps
of consistent freezing (minus 16 ° C - 2 days; minus 18
° C - 2 days; minus 20 ° C - 2 days; minus 22 ° C –
1day, minus 24 ° C - 1 day, minus 26 ° C - 1 day, minus
28 ° C - 1 day and minus 30 ° C - 8 hours). After
freezing of cuttings of each variety, the sample was
placed on the 3 days in the refrigerator at the temperature
of + 2 ° C to their gradual thawing. Cuttings were
grown at room temperature on water. As a result, obtained
by the accounting: the lowest frost-resistance
among the studied forms of parental Crimean origins
was found in grades of Shabash, Soldayya and Solnechnodolinsky;
the varieties of Dzevat kara, Kokur
bely, Kokur cherny, Misket, Misgyuli kara, Sari Pandas
were set as a weak level (-20 °C); Aybatly, Kefesiya,
Kok pandas and Ekim kara have average level up
to -22 °C; the highest frost-resistance - 24 °C – was
shown by Khersonessky and Kapselsky. Also, we have
identified synthetic frost-resistant F1-population and
plus-transgressive selection form
This article provides an overview of results of the conducting surveys on screening and saving gene pool of indigenous grape varieties in the Republic of Abkhazia, which contributed to detection and identification of the 15 previously described in the literature of Abkhazian native grape varieties
This article presents the results of genotyping of new
perspective table and technical varieties and grapes
protoclones. It was established that varieties of Citrine,
Helios, Arcadia pink and Preobragenie show differ
genetic diversity in four loci
In this article we have described our work on genotyping of new ten productive wine grape protoclones
In this article we have described a study of genotyping of new three productive wine grapes protoclones: Rhine Rieslin, Verdot black and Johanniter
Results of four-year (2004-2007) researches of
perspective technical grape varieties by contrast
zones of Krasnodar region : in central- Antaris,
Beisug, Cabernek, Clairette Temryk, Litdar, Merlok, Muscat Kuban, Rislinalk, Risling Kuban, Shardonek, in Anapa-Krasnostop AZOS, Rubin AZOS and to the memory of Zotkina, were cited in the article. It was shown their superiority under control varieties.
Ampelographical screening of gene pool was carried out in subzone Temryk on the plot of I.G. Karas’
in 2007 and in the result of which there were selected some perspective varieties: Arcadia, Victoria, Hybrid 342, Long-waited , Lora, Original.