Scientific Journal of KubSAU

Nutrient media for shoot development from shoot tips 0.5-0.8 mm with several leaf primordia in four grapevine genotypes were optimized by means of a mathematical design of experiment. Over the range of component concentrations CaCl2 had considerable the highest effect on shoot tip development compared to other components of the media. Grapevine genotypes were different in their needs for concentrations of macro-elements to optimize the process of their growth and development. Shoot development from shoot tips in each grapevine genotype as a function of macro-element concentrations in media must be described by an individual regression equation. The proposed method of result evaluation and the mathematical design of experiment may be used in physiological and agricultural research for optimization of processes affected by numerous factors. The optimized media for shoot tip development may by used for sanitation of grape plants from viruses in meristem cultures

Two methods were developed, one to assess experimental results by reducing a number of developmental parameters to the overall quality, leading to the overall quality criterion, and the other to optimize processes affected by numerous interacting factors, in-vitro plant development in this case, by applying a mathematical design of experiment. Single-bud cuttings with one leaf of two Vitis vinifera L. genotypes were excised from the central part of two-month-old in-vitro grown plants and used as explants. The explants were established on bridges of filtering paper in liquid media and on solid media. Eighteen modifications of these media contained five macro-elements, each at three concentrations, and the distribution of these macro-element concentrations followed the law of random numbers (Experiment I). Parameters characterizing arm, leaf and root development of two-month-old plants of each study genotype established on each liquid or solid medium were reduced to the overall quality of plant development. Since the study genotypes differed in the ability to grow on liquid and solid media with different macro-element concentrations and also in the ability to utilize these macro-elements, the dependence of in-vitro plant development on macro-element concentrations was described by different regression equations. That is why the regression equation describing the average outcome of plant development in the two genotypes on liquid and solid media was not as significant and the description of the process was not as adequate (determined) as regression equations calculated for each process. The regression equation which describes the dependence of the average outcome of plant development on macro-element concentrations in Experiment I is as follows: y5 = 0.027 + 0.116x22 + 0.109x2x4 + 0.106x2x3 + 0.114x4. Stepwise calculation of macro-element concentrations to optimize in-vitro plant development was done based on macro-element concentrations of the initial medium of Experiment I as starting points since that medium was best efficient for this purpose by using regression equation (y5) and algorithms of multiple curvilinear stepwise regression according to the Box-Wilson method of steepest ascent. Experiment II was undertaken where macro-element concentrations (‘steps’) were calculated in a stepwise manner to optimize in-vitro plant development of the two V. vinifera genotypes and the rootstock ‘Kober 5BB’. This led to a liquid medium and a solid one which enabled a better plant development in the three genotypes relative to the use of controls: media with ½ MS macro-elements and the initial medium whose macro-element concentrations entered as starting points to calculate ‘steps’ for optimization of in-vitro plant development. The optimized medium contained macro-elements: 318 mg l-1 NH4NO3 (x1), 1188 mg l-1 KNO3 (x2), 370 mg l-1 MgSO4 7H20 (x3) (MS), 370 mg l-1 KH2PO4 (x4), 331 mg l-1 CaCl2 (x5) (MS), and other substances at optimum concentrations adjusted earlier: ¼ MS Fe-EDTA, ¼ MS micro-elements, 20 mg l-1 myo-inositol, 0.1 mg l-1 thiamine (MS), 0.5 mg l-1 nicotinic acid (MS), 0.2 mg l-1 pyridoxine, 2 mg l-1 glycine (MS), 0.1 mg l-1 indole-3-acetic acid, 10 g l-1 sucrose and, only for solid media, 7 g l-1 Difco agar. The optimized medium may be used for propagation of virus-free plants, valuable clones and grapevine genotypes created by gene engineering. The mathematical design of experiment reported in this paper which enables stepwise optimization of in-vitro plant development may be used both in agriculture and in the food industry

The article highlights the questions of preliminary determination of the yield of vineyards, grape harvest prediction method to determine the optimal length of fruit cutting of shoots and load bushes wintering eyes, kidneys examined productivity index wintering buds (CP), which shows how many inflorescences have an average for each left eye escape when cutting to length, including the dead. It was found that the yield of grapes has a great influence the degree of differentiation of inflorescence of rudiments in the central buds overwintering buds. From the moment of laying the harvest of grapes in hibernating little eyes in the form of embryonic inflorescences until commercial harvest takes two growing seasons. During this period the generative organs of grape plants are affected by many factors of the environment. Heterogeneity was found of buds along the length of one-year matured shoots. It is expressed in the fact that many grape varieties fruitfulness buds at the base of the shoot are much lower compared with upstream eyes. A certain death of wintering buds (primarily, the main kidney) at the end of the growing season, before the autumn frosts. The main reason for the death of buds during the growing season - from defeat fungal diseases. Taking into account the percentage of deaths of buds proposed a new exponent forming embryonic kidney main inflorescence - productivity index of buds by the ratio of embryonic stems to the number of buds, including the dead. Thus, annual forecasting of grape harvest next year to cutting the bushes by the proposed method makes it possible to scientifically establish the optimal cut length of shoots and fruit load on the bush eyes. This helps to realize directly the potential of each variety and vineyard site and get maximum yields even in years with low fruit tab formations

The article presents the results of the study of yield, mechanical and biochemical composition of Merlot grape vine variety and its two clones in the conditions of Taman and Anapa subzone of the Krasnodar region. Clones differ from the maternal variety by the large number of berries in the bunch, the mass of the berry, the average mass of the bunch and the yield. Maternal variety and its clones are characterized by very close percentages of berries and combs in the bunch and by indicators of the structure, composition and structure of the bunch. By the yield of juice percentage, in different growth areas clones showed the same results as the original variety. According to the content of glucose, fructose, organic acids (wine, apple, citric, succinic), potassium, sodium, magnesium and calcium cations in the juice of berries, there is no reliable differences between the original variety and its clones. The content of dry substances, sugars, titrated acids, and the sugar-acid coefficient depended on the yield, but in general, they were favorable for the production of wine materials and juices. Merlo phanagoriiskii and Merloc are ecologically plastic and belong to the first group of varieties that retain their valuable characteristics in different areas of growth

The article presents results of studying the oenology and the biochemistry of the Cabernet-Sauvignon grapes in different areas of the Krasnodar region - Taman, Anapa, Abrau-Dyurso, Gelendzhik and Krasnodar. The yields, the mechanical composition of the grapes, the biochemical indicators of the juice of berries were compared. The cultivation zone affects the yield, the average mass of the bunch, the number and mass of berries in the bunch, the mass of the crest, but according to the percentage of berries and crest in the bunch, the bunch structure (ratio of mass of berries to mass of the ridge) does not significantly differ between growing zones. The mass of 100 berries and 100 seeds, the weight of seeds in 100 berries, the clustering index (the ratio of pulp with juice to peel) can differ in different places of growth, but with insignificant coefficients of variation. The composition of the bunch as a percentage of the total mass varies little over the growing zones: the ridges make up 5.6- 5.7%, the skin 4.9-5.2%, the seeds 4.8-4.9%, the pulp with the juice - 84.3-84.5%. Structural and berry indicators varied in the range of 5.4-5.5 and 76.84, respectively. Depending on the place of cultivation, the yield of the grape must in laboratory conditions was 75.1-75.7%. Analysis of the structure, composition and the structure of bunches showed that the mechanical composition of Cabernet-Sauvignon refers to the first group of varieties that retain their valuable characteristics when changing the growing conditions. When harvesting, the mass concentration of sugars was 19-19.8%, titrated acidity - 8.1-9.2 g / dm3, pH - 3.3- 3.4. Depending on the place of cultivation of the content of organic acids in the juice of berries was: wine - 4917-5918 mg / dm3, apple - 1926-2763, lemon - 215-416 mg / dm3. In different places of cultivation in% of the total amount of organic acids studied, the content of tartaric acid varied from 61.7 to 71.4%, apple - from 24.7 to 33.3%, lemon - from 3 to 5%. Thus, the content of organic acids in the juice of Cabernet-Sauvignon berries depends on the specific growing conditions. Depending on the growing places, the mass concentration of potassium cations is 2630-3508 mg / dm3, sodium 162-436, magnesium 171-230, calcium 185-255 mg / dm3. However, in most cases, as a percentage of the total cation content, there are no differences depending on the site of growth. Thus, the biochemical parameters of the juice of CabernetSauvignon berries depend on the specific natural conditions of the terrain and, according to these parameters, the variety belongs to the second group of varieties with less plasticity to different growing conditions

The analyze of the genetic relationship or diversity between clones was made by using the microsatellites in the groups with black berries: Cabernet-Sauvignon and Merlot, and with white berries: Muscat white, Riesling and Pinot. It was possible to approve the objective evidence on the polymorphism between these forms during this analyze. These results become the rationale for the selection, breeding and making registration of these protoclones of four above-named varieties groups in the varieties-clones for state research: Cabernek, Cabernet Fanogoriisky, Cleret temrukskii, Merlok, Pinot white, Rieslinalk, Riesling Anapa, Riesling Fanogoriisky, Muscat Temruk, Shardonek and others. These varieties-clones are accepted by State commission of Russia, they will enrich the viticulture assortment of Anapa-Tamanskay zones of Kuban, therefore Russia too

New wine grape flavored pink berry grape variety named “Radost Leonidov” breed at Athens Institute of Viticulture by P.Zamanidi, L.Troshin and P.Radchevskii in 2009 by crossing the new Moskhoragos Greek muscat variety with old European variety Traminer pink. According to morphological and biological characteristics, it can be assigned to Western European eco-geographical group of varieties. Strong growth of shoots (2,1-3,0 m). The extent of maturation of vines is very high (over the entire length, except for the top). Duration of production period from bud burst to harvest - 146-155 days. Productivity is high: 20-25 t / ha. The average weight of clusters is 260. Characterized by high winter hardiness, drought resistance and increased resistance to fungal diseases in comparison with the varieties of Vitis vinifera. Flower is hermaphrodite, fully developed stamens and gynoecium. Bunch is small, conical, sometimes winged, average density. Berry is small, spherical, pinkish in color, with a thick waxy coating. Peel of medium thickness, dense and durable. Pulp and juice with a strong varietal flavor, similar to the aroma of Traminer pink. Sugar content is very high: more than 24%. Cluster of the variety of Radost Leonidov at overripening is drying and stored on the bushes for a long time. The variety is intended for the production of dry white wines of excellent class, as well as highquality sparkling wines, dessert and sweet wines; suitable for the production of high-quality aromatic juices

New complex-steady black berry technical kind of a grape - "Aleksampelo" is breed in Greece by P.Zamanidi and L.Troshin in 2003 by mating of Firstborn Magaracha with a mixture of blossom dust of Greek black berry sorts of Bakuri and Mavrostifo. Duration of productional phase is 146-155 days. This sort is сильнорослый. A degree of ripening of a vine is very high. Productivity is high. The medial mass of racemations is 230 gr. It differs with high winter-hardiness, drought resistance and raised sustainability to fungous diseases in comparison with sorts of Vitis vinifera L.; it is tolerant to a phylloxera. A flower is bisexual. A racemation is medial, cylinder-conical, medial denseness. A bacca is medial, roundish, blue - black colour, with a dense wax coating. A peel is tight, strong. Pulp and juice with the expressed high quality flavour. Sugar content of juice of baccas is very high. The crop is maintained on bushes duratingly enough. The sort is designed for production of dry red wines of the excellent quality and also for high-grade dessert and sweet wines; it is applicable for development of high-grade juice and compotes as well

The breed of grape of Hrisi Irini is bred in Greece by P.Zamanidi and L.Troshin in 2003 by mating of the Greek breed of Asirtiko with the French breed of Semilon. Duration of production period is 156-165 days. Growth of shoots is strong (2,1-3,0). Degree of ripening of a vine is very high. Productivity is high. Average mass of clusters is 200 Differs with high winter hardiness, drought resistance and the raised resistance to fungous diseases in comparison with breeds of Vitis vinifera L. Flower is bisexual. Cluster is average, cyclo-conical, average density. Berry is average, oval, greenish-yellow colour, with a dense bloom. A pellicle is dense. Pulp and juice with high-quality odour. Sugar content is very high, it reaches 40 % with constant acidity in air-dry clusters on bushes. The breed of Hrisi Irini is included in ecological and geographical group of breeds of basin of Black sea under its morpho-physiological characteristics. The breed is intended for manufacturing dry white wines of an excellent class, and also high-quality dessert, sweet and sparkling wines; it is applicable for production of high-quality juice, compotes as well. The breed is very perspective for southern zones where grapes have no sufficient acidity

Variety Moskhoragos is hybrid one: it was bred by Panteley Zamanidi, Leonid Troshin and Leonid Maltabar in 2001 in Greece by crossing of varieties Savvatyano and Muscat Alexander. By morphological-biological characteristics it was included in a group of varieties of the Black Sea basin (convar. pontica subconar. balcanica Negr.). Variety is new and that’s why it has limited spreading. Productive period length is 156-165 days. Length of shoots is 2,1 – 3.0 m. Degree of cane maturity is high – 81-95 %. Crop capacity is 25-30 t/ha. Percentage of fruit-bearing shoots is more than 90. Average cluster mass is 300 g. It differs with high cold resistance, drought resistance and increased resistance to fungi diseases in comparison with Muscat varieties Vitis vinifera L. During blossoming it can be recognized by a little crown of greenish-yellowish color with winy-red shade in the end part, shining surface of leaves with webby downiness in the lower part. First leaf is greenish, winy-red in edges, highly downy with both sides, second and third leaves are greenish-bronze, pentalobular, slightly webby –downy in the upper side and highly downy in the lower side. Fourth and fifth leaves are green with yellowish shade, brilliant, with rare webby downiness in the upper side and highly downy in the lower side. Shoot is green, with brown-reddish shade. Leaf is symmetrical. Leaf plate surface is large, dark-green, leaf’s form is pentagonal with five lobules, bladder surface is weak. Leaf edges have middle-acuminated dens, erect by both sides. Lower lobules are slightly overlapped. Leafstalk length is average. Inflorescence is cylindrical, often with antenna. Two inflorescences usually appear on one shoot on 4 or 5 nods and inflorescences appear on the shoots, grown from resting buds on perennial wood as well. Flower is monecious, normal. Ovary is round and ball-shaped. Pollen has normal shape, fertile. Cluster is average, cylindrical, alary, with average density. Berry is middle-sized, round, yellowish-green color. Flesh is juicy, sweet, with nice Muscat after-taste. Sugar content is high. Seed has an average length, light-brown, pear-shaped, with middle-sized beak. Variety is used for preparation of high quality table, strong and dessert wines of different categories.