Scientific Journal of KubSAU

In this article we have described our work on genotyping of new ten productive wine grape protoclones

The variety "Professor Maltabar" is new, it is growth in Greece by Pantelei Zamanidi and Leonid Troshin in 2001 year from crossing of Aigeorgitico and Cabernet Sauvignon. The variety belongs to the group Black sea basin (convar. orientalis subconvar. caspica Negr.). The production period is 146-156 days. The yield is higher. The bunch weight is 200 g. The bunch is middle, cylinder conical or winged, middle density. The berry is middle, round, blue-black color. The skin is density, the pulp is juicy, the sap in not colored, with solanum after-taste. The sugarnesses is higher. The quantity of seeds is two-three. The seed is middle, grey color, piriform, with shot beak. It is higher steadiness variety for winter frost, drought-resistance and higher steadiness for mushrooms disease compare with it’s parents. The variety is used for preparing higher quality dry red, pink and white (champagne) wines of different categories.

Nutrient media for shoot development from shoot tips 0.5-0.8 mm with several leaf primordia in four grapevine genotypes were optimized by means of a mathematical design of experiment. Over the range of component concentrations CaCl2 had considerable the highest effect on shoot tip development compared to other components of the media. Grapevine genotypes were different in their needs for concentrations of macro-elements to optimize the process of their growth and development. Shoot development from shoot tips in each grapevine genotype as a function of macro-element concentrations in media must be described by an individual regression equation. The proposed method of result evaluation and the mathematical design of experiment may be used in physiological and agricultural research for optimization of processes affected by numerous factors. The optimized media for shoot tip development may by used for sanitation of grape plants from viruses in meristem cultures

Kormilitsa Lisa grape was created in Greece by P. Zamanidi and L.Troshin in 2003 with crossing the varieties of the Pervenets Maharach with varieties of Muscat white. The duration of the production period is 146-155 days. The yield is high. The average mass of clusters of 250 g. It has winter hardiness, drought-resistance and high resistance to fungal diseases in comparison with varieties of Vitis vinifera; it is also tolerant to phylloxeras. The flower is ambisexual. The bunch is average, cylinder-conic, medium density. The berry is average, oval, green-yellow colour, with a thick wax coating. The skin is thin, thick, and strong. The pulp and juice with a strong Muscat flavor. Sugar content is high with acidity constant. When overripen, it dries long and is stored on the bushes. The variety is meant for manufacture of dry white wine of superior class and high-quality dessert and sweet wines; It is also suitable for the development of high-quality juices and compotes

The grape variety with colored pulp and juice named “Professor Davids” was selected in Greece by P.Zamanidy, L.Troshin and A.Isachkin in 2003 with crossing of the Greek varieties of “Mandilaria” with the French introducent of “Alicant Bushe”. The duration of the production period is 156-165 days. Variety is full-grown. The degree of maturation of the vines is high. Crop yields are very high. The average mass of clusters is 300 gr. Has a high winter hardiness, drought-resistance and high resistance to fungal diseases in comparison with industrial varieties of Vitis vinifera. The female flower, with the stamens. The bunch of average size, cylindroconical, medium density. Berries are medium, round, blue-black in color, with a thick wax coating. The skin is thick, dense. The pulp and juice are intensively painted, with varietal flavour. Sugar content is very high. Notable for long preservation of the harvest in the bushes. The variety is suitable for making intensively painted dry red wines of superior class and high-quality dessert wines and unique juices. Due to its morpho- physiological characteristics it has been included in the eco-geographic group of varieties of the Black sea basin

In the article we have studied 55 aboriginal varieties, collected in the North Caucasus. We have described allelic diversity and identified four haplotypes. All the populations were closely related while being examined using a factor of Nei's genetic distance (Dn), with the exception of indigenous varieties of Armenia. Maximum differences have been found between the Uzbek population and Astrakhan, Turkmenian, Armenian populations. The most genetically rich were the population of Don area and Krasnodar region

The morphometry of leaves of the Kuban wild-growing lianas of grape, as we know, is not learnt till now and availability of quoters of pure subspecies of Vitis vinifera silvestris Gmel is not demonstrated., though suppositions about this information soared in the scientific environment as old as Adam [5]. With the advent of a direction of morphometry taxonomy there was a necessity of accurate bracing of a current state of a genotypical diversity of lianas and biological polymorphism of leaves for link establishment silvestris - silvesatis - sativa

Newest wine black berry with painted pulp and juice grape variety called Mir was breeded at Athens Institute of Viticulture (Greece) by Zamanidi P., Troshin L. and Radchevsky P. in 2006 by crossing the Ukrainian variety of Odessa black (Alicante Bouschet x Cabernet Sauvignon) with new Greek black berry variety Iliad. According to the morphological and physiological characteristics included in the eco-geographical group of Black Sea Coast. Length of production period - 146-155 days. Strong growth of arms (2,1-3,0 m). Percentage of productive shoots - over 90. Productivity is very high: 20-30 t / ha. The average mass of clusters is 260 g. Characterized by high resistance to cold, drought and increased resistance to fungal diseases in comparison with varieties of Vitis vinifera. Lobes, the stamens and fully developed gynoecium. Bunch medium, often with tapered wing, the average density. Berry small, round, blue-black, with a thick waxy coating. The peel is medium thickness, dense, durable. Pulp and juice intensely colored. Sugar content is very high. Prolonged preservation of different crop on bushes. Variety intended for manufacturing intensely colored dry red wines of excellent grade and high-quality sparkling, dessert, sweet and liqueur wines; and is suitable for the manufacture of high quality strong colored juices. When used in blends with other varieties, it enhances color, flavor and improves the bouquet of wine

In the present article, we have described data of comparative ampelography of biometric evaluation of leaf parameters of the three table grapes: Preobragenie, Victor and Jubiley Novocherkasska, widespread in the amateur and farming areas of Russia and the Ukraine

Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA