Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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1160 kb

FEATURES OF THE ASSIMILATION APPARATUS FORMATION OF WOODY PLANTS IN THE CONDITIONS OF THE LIPETSK INDUSTRIAL CENTER

abstract 1281704022 issue 128 pp. 319 – 329 28.04.2017 ru 35
The article presents data on the growth of an assimilation apparatus of Scots pine, silver birch and pedunculate oak in the conditions of the Lipetsk industrial center. It is established that the growth dynamics of annual and biennial needles of Scots pine under pollution is higher compared to the control, while absolute values of length and mass of needles less. Starting from the second year, in the conditions of contamination noted stunted pine needle growth, which increases by the third year. It is shown that the linear dimensions of the lamina of birch in the conditions of pollution are not changing much compared to the control, whereas the linear dimensions of the lamina of pedunculate oak in the conditions of pollution is less than the control
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ECOLOGICAL PATH "CHOCHUR-MURAN" ON THE NATURAL TERRITОRY OF YAKUTSK BOTANICAL GARDEN

abstract 1281704028 issue 128 pp. 386 – 402 28.04.2017 ru 32
The article presents the data about flora and vegetation of the territory of the Yakut Botanical garden, where an ecological trail will be; there is a characteristic of plant communities and their classification. Preliminary, the flora of the ecological path includes 310 species of higher vascular plants belonging to 54 families and 172 genera
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BIOMORPHOLOGIC AND THERAPEUTIC FEATURES OF CAPPARIS HERBACEA L. SPECIES IN THE FLORA OF THE NAKHCHIVAN AUTONOMOUS REPUBLIC OF AZERBAIJAN

abstract 1281704034 issue 128 pp. 466 – 473 28.04.2017 ru 35
The article provides information about phytochemical investigation, medical and nutritional importance of fruits of Capparisherbacea L. including CapparaceaeJuss. family in the area of the Nakhchıvan Autonomous Republic. Some solvents had been applied, fruit and leave extracts had been bought. The purification and identification of content of plant extracts had been investigated with spectroscopic and chromotographic methods. The saponins, carotinoids, chlorophyll pigments and flavonoids had been revealed in the content of extracts. The extracts have been acquired from fruits of this species by use of polar and nonpolar solvents and the composition of extracts have been studied with different applied methods. The column and thin layer chromatography carried out in order to purification of contents and extracts were fractionation and Rf values were calculated
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THE CONCEPT OF THE «RNA WORLD»: THEORY AND PRACTICE

abstract 1281704053 issue 128 pp. 740 – 770 28.04.2017 ru 22
The review examines research unusual properties of RNA. RNA has the ability to act as both genes and enzymes (ribozymes). This property could offer a way around the «chicken-and-egg» problem: genes require enzymes; enzymes require genes. Furthermore, RNA can be transcribed into DNA, in reverse of the normal process of transcription. These facts are reasons to consider that the RNA world could be the original pathway to cells. The general notion of an «RNA World» is that, in the early development of life on the Earth, genetic continuity was assured by the replication of RNA and genetically encoded proteins were not involved as catalysts. There is now strong evidence indicating that an RNA World did indeed exist before DNAand protein-based life. RNA has multiple functions. Among these, "messenger RNA" carries genetic information from DNA to protein formation. RNA is often a single-stranded spiral, but also exists in double-stranded form. In 1998, Craig Mello and Andrew Fire discovered through their studies of the roundworm C. elegans a phenomenon dubbed "RNA interference". In this phenomenon, double-stranded RNA blocks messenger RNA so that certain genetic information is not converted during protein formation. This "silences" these genes, i.e. renders them inactive. The phenomenon plays an important regulatory role within a genome. Recent years have been perhaps the most fruitful period yet in terms of research in the area of mRNA stability (Phenomena: Gene Silencing; RNA interference; Identity of mRNA decay in vivo and in vitro). The elaboration of new methods in biothechnology have been presented
268 kb

REGENERATION PROPERTIES OF CUTTINGS OF STOCK OF PHYLLOXERARESISTANT GRAPE VARIETIES UNDER THE INFLUENCE OF TREATMENT BY THE ROOTFORMING STIMULATOR RADIX PLUS

abstract 1281704055 issue 128 pp. 785 – 813 28.04.2017 ru 18
Researches were carried out on three-eyed cuttings of stock of phylloxera-resistant grape varieties Berlandieri×RipariaCober 5BB (Cober 5BB), Riparia× Rupestris101-14 (101-14), Berlandieri × RipariaСО-4 (СО-4), Gravesak and RSB. Cuttings of presented varieties were wetted during 24 hours in water and after preliminary drying of the surface were covered in the upper part with antitranspirant, under the temperature about 90°С. Then 40 cuttings of every variety were placed with lower ends for 24 hours in 0,01% heteroauxin solution or for 8 hours in 1% Radix Plus solution. Cuttings of the control variant were placed in water. After treatment cuttings, were couching in a film greenhouse on a heated rack in humid steamed sawdust. In the result of the present researches there was determined that the wetting of basal ends of cuttings of stock phylloxera-resistant varieties in 1% Radix Plus water solution plus during 8 hours leads to the essential activity of regeneration processes in them. The expansion of the length of shoots on rooted cuttings of the experimental variant amounted for 19,2–154,5 %, the rooting of cuttings - 23,3–76,7 %, the output of cuttings having at least three roots – 33,0–78,1 %, the number of calcaneal roots– 80,8–257,1 %. The maximum increase of the length of shoots under the influence of Radix Plus was observed at varieties of Gravesak and СО-4, the rooting – Cober 5BB and CO-4, the amount of cuttings at least three roots and average amount of roots on them –101-14 and Cober 5BB. The Radix Plus caused the great stimulating influence the rootforming ability of cuttings of stock varieties than heteroauxin, the standard stimulator of root-forming
287 kb

VISUAL PHENOTYPING IN PLANT BREEDING

abstract 1281704071 issue 128 pp. 1038 – 1050 28.04.2017 ru 21
The article is devoted to visual analysis and its application in the selection process. New possibilities of application of visual display of information in the research process are considered. We have presented the examples of the use of visual phenotyping in breeding, evaluated the advantages and disadvantages in this area. In particular, the algorithm of the program of LHDetect for estimating leaf pubescence of wheat wafers is described, based on the analysis of digital images of the sheet bend. We have also considered Phenotiki platform that uses digital images of the profile of plants for visual phenotyping to assess plant growth. Based on the analysis of various studies it was noted that the application of visual analysis for some purposes is very effective, but for others, on the contrary, not effective. We highlight the actual problems of proper representation and converting information. The article shows the various causes of loss and distortion of data, as well as possible solutions to these problems. The work describes the application of ASK-analysis in the selection process. As an example, it describes the evaluation of the polymorphism of variety groups of grapes. We have presented interesting solutions and ways to minimize distortion and loss of information in the process of its transformation found by the authors of these works. The article reveals the concept of "computational biology" and describes fundamental differences from other areas as well as tasks, problems, possible research areas and promising directions of development of computer biology
574 kb

INTENSIFICATION OF THE CULTIVATION PROCESS OF PHYSIOLOGICALLY-ADAPTED LACTOBACILLI AS A BASIS FOR THE CREATION OF BIOLOGICAL PRODUCTS OF MICROBIAL ORIGIN FOR POULTRY BREEDING

abstract 1281704076 issue 128 pp. 1101 – 1114 28.04.2017 ru 20
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
737 kb

"RARE PUMPKIN" IN STAMPS – THE HISTORY OF PLANTS, DISTRIBUTION AND ICONOGRAPHY

abstract 1271703011 issue 127 pp. 204 – 221 31.03.2017 ru 95
In the article we consider illustrative images of rare pumpkin cultures presented in stamps of different countries. In this work, we analyze stamps which represent rare pumpkin cultures. Stamps are reflection of the economy; they portray agriculture and elements of technology of agricultural production. Cucurbitaceae family includes a large number of species that are very different from each other in the form of fruits, in purpose, in origin. The objective of our study was to conduct a visual analysis of rare pumpkin crops, which are used in the world; to reflect the research progress on the introduction and the achievement of breeding work. The article provides an analysis of several cultures: momordika (Momordica charantia L.), Kiva (Kiwano), horned melon, cucumber Antilles, (Cucumis metuliferus L.); Akantositsios Naudin (Acanthosicyos naudinianus L.); momordica balsamina (Momordica Balsamina L.); koktsiniya (Coccinia sessifolia L.); luffa cylindrical (Luffa cylindrica L.); Lagenaria (Lagenaria siceraria (Molina) Standl.); cucumber snake (Trichosanthes anguina L.); antilles cucumber, anguria (Cucumis anguria L.); african cucumber (Cucumis africanus Lindl); wax gourd (Benincasa hispida (Thunb) Cogn..); chayota or mexican cucumber (Sechium edule Swartz). The analysis of the iconography of images of rare pumpkin crops in stamps of different countries allowed us to see the history of culture introduction of wild pumpkin from the local and foreign flora
164 kb

EFFECT OF GOLD NANOPARTICLES ON SEEDS GERMINATION OF WINTER BARLEY

abstract 1271703018 issue 127 pp. 295 – 307 31.03.2017 ru 113
The article presents experimental data on the research of gold nanoparticles (GNPs) with mean diameter 15 nm and 50 nm effect on seeds germination and growth of 3-day-old etiolated coleoptiles and roots. Compared with distilled water (control) initial colloidal solution of 15 nm and 50 nm GNPs (57 µg/ml) had no significant impact on seeds germination and growth of coleoptiles and roots. However, in both cases a weak tendency to stimulation of the coleoptile growth and root growth inhibition was observed. Reduction of 15 nm GNPs concentration down to 10 µg/ml and 1 µg/ml had not effect on the growth of the seedlings, but stimulated seed germination up to twofold. Similar concentrations of 50 nm GNPs exerted the stimulating effect on seed germination (twofold) and the growth of root and coleoptiles. Seedlings grew especially intensive in colloidal GNPs solution with Au concentration of 10 µg/ml. Temperature rise of 2 degrees (from 25°C to 27°C) resulted in growth increase of control 3-day-old seedlings and opposite effect of 50 nm GNPs: coleoptiles and roots growth fell behind control seedlings growth by 16-17%. However, on the 4th day, the relative growth slowdown of control seedlings occurred at 27°C and growth stimulation effect under the influence of 50 nm GNPs appeared again. Over time, the stimulating effect of 50 nm GNPs decreased: at the end of October it weakened, and in November – beginning of December, it was no observed for the roots, and there was a decline in coleoptiles growth. However, in all cases the effect of stimulation of seeds germination persisted under the influence of 50 nm GNPs, weakening by December. We hypothesize the molecular mechanisms of biological action of GNPs
175 kb

MORPHOLOGICAL VARIABILITY OF IRIS HALOPHILA AN INTRODUCTION IN CENTRAL YAKUTIA

abstract 1271703048 issue 127 pp. 713 – 722 31.03.2017 ru 97
Investigation variability of morphological features of Iris halophila an introduction in Central Yakutia. Dependence of morphological variability of Iris halophila on quantity of dropping-out rainfall in initial habitats is revealed
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