Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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207 kb

APPROBATION OF SSR-ANALYSIS FOR DNA-IDENTIFICATION OF COMMERCIAL WINE YEAST STRAINS

abstract 1251701009 issue 125 pp. 151 – 163 31.01.2017 ru 64
The study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
2071 kb

MONITORING OF RARE PLANT’S SPECIES POPULATIONS IN THE TERRITORY OF NATURE MONUMENT CALLED «НERB-FESCUE-FEATHER GRASS STEPPE» (ROSTOV REGION)

abstract 1251701018 issue 125 pp. 258 – 273 31.01.2017 ru 56
In the article we have stated results of the monitoring of local populations of rare plans species in the territory of the nature monument (NM) of the Rostov region «Raznotravno-tipchakovo-kovylnaya steppe» (Zernogradsky district). Vegetation of NM belongs to the poor version of the Azov steppe, flora contains 291 species, including 13 rare species (Astragalus ponticus Pall., Bellevalia sarmatica (Pall. ex Georgi) Woronow, Calophaca wolgarica (L. f.) DC., Caragana scythica (Kom.) Pojark., Centaurea talievii Kleop., Crambe pinnatifida R. Br., C. tataria Sebeok, Crocus reticulatus Stev. ex Adam, Eriosуnaphe longifolia (Fisch. ex Spreng.) DC., Iris pumila L., Stipa pulcherrima К. Koch, S. ucrainica P. Smirn., Tulipa schrenkii Regel), included in the Red List of the Rostov region, including 6 species included in the Red List of the Russian Federation. In 2016 were specified the patterns of rare species distribution in NM borders, and also other important parameters of their populations (number, density, age structure, etc.). The group of rare plants species, included in the Red List of the Rostov region, in the territory of NM includes 65 % of their total number known in Zernogradsky district. The special sozological value of NM is determined by the large local populations of Calophaca wolgarica, Astragalus ponticus and Crambe pinnatifida. Local populations of all rare species in the territory of NM can be considered to be steady as inhabit here during last 50 years
219 kb

REGULATION OF THE CROP AND QUALITY OF GRAPES OF GRADE RIESLING BY USING VARIOUS TECHNOLOGICAL SCHEMES OF NUTRIVANT PLUS NON-ROOT FERTILIZING

abstract 1251701044 issue 125 pp. 658 – 679 31.01.2017 ru 55
Researches on non-root additional fertilizing of grapes with complex water-soluble fertilizer called Nutrivant plus have been conducted in corporation "Pobeda" in Temrjuksky area of Krasnodar territory on the implanted plantings of technical grade Rhine Riesling. In the test, the following kinds of Nutrivant plus were used: oleiferous (N0P20K33), sugar beet (N0P36K24), grapes (N0P40K25), universal (N19P19K19), grain (N6P23K35). Each mark of Nutrivant plus contained a certain set of microelements. The test consisted of six variants:1 variant - without additional fertilizing(control); 2 variant: grapes - before flowering, grapes - a growth phase of berries (a berry about a pea), oleiferous - the beginning of maturing of berries; 3 variant: universal - before flowering, universal - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 4 variant: grain - before flowering, grain - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 5 variant: Sugar beet - before flowering, a sugar beet - a growth phase of berries (a berry about a pea), a sugar beet - the beginning of maturing of berries; 6 variant: universal - before flowering, grain - a growth phase of berries (a berry about a pea), grapes - the beginning of maturing of berries. Norm of the expense of fertilizer is 3 kg/hectares. The expense of a working liquid - 700 l/hectares. The result was, that in the third, fourth and fifth variant of the test the increase in a crop from a bush happened accordingly on 7,5, 13,8 and 17,8 %. The maximum increase of a crop in the fifth variant was accompanied by increase in mass concentration of sugars in juice of berries on 0,6 g/100 см3и decrease titratable acidities for 0,6/dm3 As a result triple not root additional fertilizing of grapes plants grade Riesling with Nutrivant plus (universal - before flowering, grain - in a growth phase of berries and grape - in the beginning of maturing of berries) promoted more optimization of a water mode of leaves, increase in weight of a cluster, a crop from a bush and productivity, mass concentration of sugars and phenolic substances in juice of berries
3033 kb

RESULTS OF TEN YEARS BREEDING WORK ON DEVELOPING TOMATO VARIETIES AND F1 HYBRIDS FOR GROWING IN THE OPEN FIELD IN THE KUBAN REGION

abstract 1241610043 issue 124 pp. 684 – 694 30.12.2016 ru 53
Tomato is one of the most popular vegetables in Kuban. Every year, the area under this crop in the region occupies more than 11 thousand ha. Currently the State Register of Breeding Achievements approved for use in Krasnodar region, includes more than 90 tomato varieties and hybrids, however, the need for new, more productive varieties is not reduced. The important task of breeding is development of varieties and F1 hybrids with stable realization of the potential, with high level of yield ability, resistance to adverse environmental factors. In the southern regions of Russia for tomatoes, especially during flowering and fruit ripening, the most unfavorable abiotic environmental factors are excessive heat (air temperature 35 - 45 ° C or higher) and high solar radiation. Adverse temperature conditions often act as a deterrent in obtaining high yields, due to the flowers and fruits drop. The article presents the results of breeding activities of the last ten years (2007 - 2016) on development of new tomato varieties and F1 hybrids for different purposes As a result of studies there were developed four tomato varieties and one F1 hybrid that meet the requirements of production and are adapted to abiotic conditions of cultivation in open field in Kuban
178 kb

VITALITY AND VARIABILITY OF MORPHOLOGICAL CHARACTERS LILIUM PENSYLVANICUM KER-GAWL. IN THE NATURE AND CULTURE

abstract 1241610055 issue 124 pp. 840 – 851 30.12.2016 ru 51
The article presents the results of studies of vitality and of the correlation variability of morphological traits cenopopulations Lilium pensylvanicum Ker.- Gawl. on the natural territory of the Yakutsk Botanical Garden and in cultura. The study shows variability of their parts (variability) and the estimation coenopopulations vitality (vitality)
383 kb

APPROBATION OF SSR MARKERS DEVELOPED FOR THE SPECIES OF PRUNUS PERSICA, IN RELATION TO DISCHARGE PLUM

abstract 1241610098 issue 124 pp. 1479 – 1490 30.12.2016 ru 52
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
3641 kb

CREATION OF THE GENERALIZED IMAGES OF GENUS OF BUGS OF GROUND BEETLES (COLEOPTERA, CARABIDAE) ON THE BASIS OF THEIR TYPES IMAGES, USING THE ASKANALYSIS METHOD

abstract 1231609002 issue 123 pp. 30 – 66 30.11.2016 ru 75
In this article we consider application of the automated systemic and cognitive analysis (ASK-analysis), its mathematical model – a systemic information theory and the program tools realizing them – the intellectual Eidos system, for input (digitization) of images from graphic files, synthesis of the generalized images of classes, their abstraction, classification of the generalized images of classes (clusters and constructs), comparison of concrete images with the generalized images (identification) of classes, comparisons of classes with each other and creations of the generalized images of genus of ground beetles on the basis of images of the types. The new approach to digitization of images of ground beetles based on use of a polar frame, the center of weight of the image and its external contour is offered. Before digitization of images, their transformations standardizing the provision of images, their sizes and an angle of rotation can be applied. Therefore, the results of digitization and the ASK-analysis of images can be invariant (are independent) concerning their situation, the sizes and turn. There is a successful experience of the solution of similar tasks in other subject domains. This article can be considered as a continuation of series of the works devoted to application of the automated systemic and cognitive analysis (ASK-analysis) and its program tools – the Eidos system
154 kb

CHRONIC TOXICITY OF IMIDAZOLINONE HERBICIDE CALLED IMAZETHAPYR TO FRESHWATER ORGANISMS OF VARIOUS SYSTEMATIC GROUPS

abstract 1231609005 issue 123 pp. 90 – 101 30.11.2016 ru 102
The article presents the results on the effects of Imazethapyr herbicide on aquatic organisms. The chronic toxic effects have been studied of the imidazolinone herbicide in a concentration range of 0.4 - 1150 mg/l to the representatives of various groups of aquatic organisms: zoobenthos – great ramshorn snail (Planorbarius corneus), zooplankton - Daphnia (Daphnia magna Straus), phytoplankton - protococcal alga (Scenedesmus guadrigauda) and macrophytes - higher aquatic vegetation (Elodea canadensis Michx). It is shown that the herbicide does not have a pronounced lethal effect to aquatic organisms tested. Their respective LC50 values are > 1000, 680, 510 and 910. Elodea is shown to be most sensitive to its sublethal effects (decreased growth of lateral and main sprouts at a concentration of 10 mg/l and more), reduced fertility was observed in Daphnia at a concentration of 300 mg/l and above, and as to the great ramshorn snail no sublethal effects have been revealed for all the concentrations studied
1326 kb

INTRODUCTION OF NEW GRAPES VARIETIES TO THE IN VITRO CULTURE

abstract 1231609023 issue 123 pp. 339 – 346 30.11.2016 ru 89
Clonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way
156 kb

THE APPLICATION OF THE BACTERIOPHAGES TO COMBAT COLIBACILLOSIS AND CAMPYLOBACTERIOSIS IN POULTRY

abstract 1231609029 issue 123 pp. 421 – 432 30.11.2016 ru 39
This work considers the needs and methods of phage therapy of two bacterial infections in poultry. The review examines the use of bacteriophages, from the moment of their discovery to the present day. We discuss the relevance of such diseases as compilobacteriosis and colibacteriosis transmitted from birds to humans, we show how to use bacteriophages and their efficacy in models of adult birds and chickens, including information provided on the pilot study of phage therapy at a poultry farm. The reported study was partially supported by RFBR, research projects No. 16-44-230855 and No. 13-04- 00991 and № 16-44-230855-р_а
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