The article presents the first results of studies of
somaclonal variability, which take place during
cultivation of tea plants tissues and organs in vitro
culture. As a starting material, there was used
morphogenic callus, isolated from the basal part of tea
microshoots, because callus increases somaclonal
variability. An optimized protocol of the nutrient
medium for induction gemmogenesis to product somatic
clones of tea plants in vitro culture was developed.
Morphogenesis calluses, initiated from the basal part of
tea microshoots and subcultured on the nutrient medium
with 6 – BAP – 2,5 ml + gibberellic acid – 1,0 ml +
Tidiazuron – 4,0 ml + Tryptophan – 1000 mg,
distinguished by high rates of induction of
gemmogenesis – 63,3 %. The article studies the effect of
exogenous growth regulators on morphological and
growth indices of tea callus culture in vitro
The pathogenic agent of apple scab disease, Venturia
inaequalis (Cooke) Winter, causes significant damage
to the industrial production of apples. Studying the
pathogen in pure culture is important for solving the
theoretical and practical issues of its biology. The
sporulation ability of the Venturia inaequalis
laboratory culture is an important diagnostic feature in
morphological culture analysis, in the test for
sensitivity to fungicides, when creating a collection
inoculum for an artificial infection. We have studied
the morphological features of conidiogenesis of pure
V. inaequalis culture, such as the location of
conidiogenic structures and their form. For the first
time in the laboratory culture of the pathogen,
sporulation in the thickness of agar on the substrate
mycelium was demonstrated. Conidiogenesis
proceeded according to the blastic-annellidic type.
Depending on the location of the conidiogenic
structures on the aerial or substrate mycelium, their
morphology was different. Conidiogenic areas in the
substrate mycelium could be observed with the
unaided eye, in the form of hyphal grit, while they
were conidia conglomerations in each annelid.
Annellide had a curved shape. On the aerial mycelium,
annelids were straight and always had only one
conidium. Differences in the morphology of
conidiogenic structures are supposed to be related to
the physical conditions of the environment in which
sporulation takes place. In the agar, each mature
conidium remaining at the apex of the annelid
interferes with the formation of the next one, which
results in its bending. The fixed arrangement of
conidiogenic structures and forming conidia in the
thickness of agar allows the use of substrate
sporification for model studies of the conidiogenesis
process
The work was carried out in the scientific-research
laboratory of the chair of Biotechnology, Biochemistry
and Biophysics of Kuban SAU, the aim of which is the
search of optimal conditions of obtaining the
hydrolyzates of lactic acid bacteria by thermoacid and
enzymatic ways. The pure cultures of microflora of
gastrointestinal tract of quails − Lactobacillus agilis,
Lactobacillus intermedius and Lactobacillus salivarius
were the object of researches. Under conducting the
thermal acid hydrolysis there were used the same
correlations of bacterial mass and water as well as
procedures of clearing of hydrolyzates. The variable
conditions were рН, temperature and time of hydrolysis.
When carrying out the enzymatic hydrolysis in this
study there was used the enzyme from the group of
muramidase lysozyme. The amount of enzymes and
time of carried out hydrolysis were the variable
conditions under obtaining the enzymatic
hydrolyzates. In the process of researches there were
studied: qualitative content of total protein, peptides
with М.м. < 1500, protein and peptides with M.m. >
1500, aminoacids and as well as GMDP
(glucosaminilmuramilpentapeptide). On the basis of
carried out experiments there was stated that according
to all components of biologically active substances the
thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates
by different ways was revealed at destruction of cells
Lb. intermedius. Content of GMDP in thermal acid
hydrolyzate was higher than in enzymatic on
0,028 g/100 ml (51,8 %)
The phenomenon of fasciation on the example of a higher plant called “Taraxacum officinale Wigg” has been considered. The effect of fasciation on the various particularities of plants has been shown. There are numerous examples of the different fasciation types of present plant’s type which are growing in different ecological areas. We have demonstrated that the phenomenon of fasciation exists in natural populations and in areas with development pressures, and it may occur after mechanical damage to plants of Taraxacum officinale. In most cases, fasciation is regarded as an exceptional phenomenon. A number of authors have distinguished fasciation to non-inheritable and inheritable. The first is the influence of external factors such as insect damage, injury, weather conditions. The second is caused by internal reasons. The number of regularities was installed that the reproductive bodies of plants are more often fascinated, and thermophilic forms are more likely to have fasciation. Photographic images of various types of fasciation, patterns and diagrams are given. Fasciation can be considered as a marker because of the connection of this phenomenon with the violation of the ecological situation. Plants with morphoses including ones with fasciation of individual organs, can act as indicators of pollution of the natural environment. Thus, the phenomenon of fasciation affects many areas of science, such as ecology, morphogenesis, genetic monitoring. The possibility of studying the phenomenon of fasciation on a model object of dandelion is shown. As a methodical approach to the study of this phenomenon, it is proposed to create a database of images, in this way it would be cataloging the image
The article presents the results of the genetic analysis of the trait ‘plant height’ for six crosses involving the forms related to different subspecies of rice. There have been estimated the types of inheritance and a number of genes, which assist to determine this quantitative trait. We have found out that the variance of the trait ‘plant height’ is determined by 3-5 pairs of genes having additive and dominant activities and the degree of dominance reduces in case the variance among parental forms increases. The non-allelic interaction of the genes of the initial forms produces transgressive dwarf and tall forms. The cross ‘Lampo’x’Virazh’ showed the highest degree of transgression in ‘plant height’ due to short height of both parental forms (61.7%), but the frequency of transgression was larger than that of the cross ‘Lampo’x’Komandor’(17.6%). This combination (‘Lampo’x’Komandor’) produced more tall forms (up to 135 cm). The segregation of a great number of tall forms into F2 testifies that the parental forms of these hybrids vary in the allelic state of several pairs of genes, whose various combinations form phenotypes with a longer stem. Thus, the semi-dwarf feature of the varieties ‘Lampo’, ‘Komandor’ and ‘Virazh’ is determined by various non-allelic genes
In the Southern Federal University on the genetic basis
of sunflower inbred line 3629, a collection of plastid
mutants with varying degrees of chlorophyll
deficiency was created by inducing Nnitrosomethylurea.
Chlorophyll content was associated
with their photosynthetic activity. It was found that the
lower the chlorophylls content in mutant plastids, the
lower the sugar concentration in plant tissues. For
example, during the entire period of plant growth for
whites (1.0-3.0 % chlorophylls a+b from control) var-
10, var-17 and yellows (6.0-9.5 % chlorophylls a+b
from control) var-29, var-33 leaf areas of variegated
mutants depending on the development phase and the
content of green pigments are characterized by a low
(2-7 fold) sugar content. Yellow-green (75.5%
chlorophylls a + b from control) leaves of en:chlorina-
7 contain a higher level of carbohydrates, although it is
1.5-2 fold lower than at line 3629. Monosaccharides
are products of hydrolytic reactions catalyzed by β-
glycosidases. It was shown, that the activity level of β-
galactosidase and β-glucosidase exceeded the
corresponding indicators of the control at 1.5-2 and 2-7
fold, respectively. Similarly to enzymes from the
water-soluble fraction, membrane-bound β-
glycosidases also showed increased activity in the
leaves of the investigated mutants, compared to the
control green plants of 3629. Consequently, the
activity of β-glycosidases increases dramatically in
leaf tissues with deficiency of photosynthetic. Thus,
chlorophyll mutations can lead to a change in the
expression of nuclear genes, resulting in a significant
increase in the activity of β-glycosidases in the mutant organelles themselves
The results of the introductory test of 22 species of the
family Caprifoliaceae from 5 genera are analyzed:
Abelia R. Br. (1 species), Diervilla Mill. (1 species),
Kolkwitzia Graebn., Lonicera L. (15 species),
Symphoricarpos Ducham. (2 species), Weigela Thunb.
(2 species) in the Botanical Garden of SFedU. The
estimation of ecological-biological properties, of
degree of naturalization is given, of phenological
development, of age status, of decorative longevity of
these species. It has been established that 17 species
have high winter hardiness: Kolkwitzia amabilis;
Lonicera caprifolium, L. coerulea, L. chrysantha, L.
demissa, L. dioica, L. ferdinandii, L. gracilipes, L. ×
heckrotii, L. japonica, L. morrowii, L. tatarica, L. ×
tellmanniana, L. trichosantha, Symphoricarpos
hesperius and S. occidentalis, Weigela praecox;
drought-resistant: Abelia × grandiflora, Kolkwitzia
amabilis; Diervilla rivularis, Lonicera acuminata, L.
caprifolium, L. confusa, L. chrysantha, L. demissa, L.
ferdinandii, L. × heckrotii, L. japonica, L. morrowii, L.
tatarica, L. × tellmanniana, L. trichosantha,
Symphoricarpos hesperius and S. occidentalis,
Weigela floribunda; medium-drought-resistant:
Lonicera coerulea, L. dioica, L. gracilipes, Weigela
praecox. Period of preservation of decorative qualities
in plant ontogeny: Abelia × grandiflora - not less than
10 years; Diervilla rivularis, Lonicera dioica, Weigela
praecox - 15-20 years; Lonicera demissa, L. gracilipes,
Weigela floribunda - 20-25 years; Lonicera acuminata,
L. caprifolium, L. coerulea, L. confuse, L. chrysantha,
L. etrusca, L. ferdinandii, L. × heckrotii, L. japonica,
L. morrowii, L. tatarica, L. × tellmanniana, species of
Symphoricarpos – 25-30 years, Kolkwitzia amabilis –
30–35 years, Lonicera trichosantha 35–40 years
In the present study, fauna of small mammals of the
Taman Peninsula is analyzed. It is shown that the
complex of rodents and insectivores inhabiting the
study area differs considerably from the adjacent
continental areas and is an isolate. Structure of its
fauna and population resembles that of the steppe parts
of Crimea and, at the same time, North-West NearCaspian
areas. Analyses of variation of the D-loop
regions of mtDNA in yellow-bellied mouse Sylvaemus
witherbyi, as well as certain peculiarities of the species
diversity, show the priority of faunal and genetic
connections of the Taman Peninsula’s mammal fauna
with the North-West Near-Caspian one
In the presented study, we have performed genotyping
of modern Russian rice cultivars using microsatellite
DNA-markers. The markers showed different level of
allelic polymorphism: from 2 to 8 alleles per locus. For
all studied cultivars,unique DNA-fingerprints were
obtained
The article shows the direct impact of oil pollution on
morphometric parameters and external a large burdock
broadleaf and camel spines ordinary. The object of the
study is Sokolovsky oil wells in the Astrakhan region.
Vegetation is very sensitive to violations environment
and most visually reflects the changing ecological
situation territory as a result of anthropogenic impact.
Petroleum products concentration of 8100 mg / kg
leads to a decrease some morphometric indicators
plants and deterioration of their external condition.
The results of content research oil products in the soil
showed that the maximum values of this indicator at
the end of the oil wells (near rescue station), which
was more than 8 ODK, the minimum - in control, in
the region beach (0.1 ODK), at all other points. The
indices were within the norm (1000 mg / kg). In the
location of the oil there were 10 terrestrial plant
species. We have studied plant territories using the
following parameters: root length, thickness, the length
of the above-ground part