The article presents the results of studies of vitality
and of the correlation variability of morphological
traits cenopopulations Lilium pensylvanicum Ker.-
Gawl. on the natural territory of the Yakutsk Botanical
Garden and in cultura. The study shows variability of
their parts (variability) and the estimation
coenopopulations vitality (vitality)
Microsatellite DNA markers are currently used
effectively in the study of the genetic diversity of the
gene pool of fruit crops and DNA certification of
varieties. For plum now there is rather limited list of
works on the development of this type of DNA
markers. Most often, the search for new SSR-markers
for this species is carried out by checking of crossreproducibility
of SSR-markers developed in other
species of the genus Prunus. In this study, for the 18
SSR-markers previously developed on a peach, there
was performed testing and evaluation of the prospects
for the use of the genotyping of plum cultivars. Testing
was made on the 4 varieties of genetically distant,
belonging to the 4 different subspecies of Prunus
domestica L., showed the effectiveness of their use.
During the study, all tested DNA-markers were
grouped together in multiplex sets comprising 3-4
markers. This allows simultaneous genotyping of 3-4
loci in a single PCR reaction. These multiplex kits are
available for use in the study of genetic polymorphism
of species Prunus domestica L
In this article we consider application of the automated systemic and cognitive analysis (ASK-analysis), its mathematical model – a systemic information theory and the program tools realizing them – the intellectual Eidos system, for input (digitization) of images from graphic files, synthesis of the generalized images of classes, their abstraction, classification of the generalized images of classes (clusters and constructs), comparison of concrete images with the generalized images (identification) of classes, comparisons of classes with each other and creations of the generalized images of genus of ground beetles on the basis of images of the types. The new approach to digitization of images of ground beetles based on use of a polar frame, the center of weight of the image and its external contour is offered. Before digitization of images, their transformations standardizing the provision of images, their sizes and an angle of rotation can be applied. Therefore, the results of digitization and the ASK-analysis of images can be invariant (are independent) concerning their situation, the sizes and turn. There is a successful experience of the solution of similar tasks in other subject domains. This article can be considered as a continuation of series of the works devoted to application of the automated systemic and cognitive analysis (ASK-analysis) and its program tools – the Eidos system
The article presents the results on the effects of
Imazethapyr herbicide on aquatic organisms. The
chronic toxic effects have been studied of the
imidazolinone herbicide in a concentration range of
0.4 - 1150 mg/l to the representatives of various
groups of aquatic organisms: zoobenthos – great
ramshorn snail (Planorbarius corneus), zooplankton
- Daphnia (Daphnia magna Straus), phytoplankton -
protococcal alga (Scenedesmus guadrigauda) and
macrophytes - higher aquatic vegetation (Elodea
canadensis Michx). It is shown that the herbicide
does not have a pronounced lethal effect to aquatic
organisms tested. Their respective LC50 values are
> 1000, 680, 510 and 910. Elodea is shown to be
most sensitive to its sublethal effects (decreased
growth of lateral and main sprouts at a concentration
of 10 mg/l and more), reduced fertility was observed
in Daphnia at a concentration of 300 mg/l and
above, and as to the great ramshorn snail no
sublethal effects have been revealed for all the
concentrations studied
Clonal micro-multiplication – is this the basic method
of obtaining the qualitative virus-free planting
material. The high quality specificity of the explants
reaction of the grapes varieties to the composition of
nutrient medium requires the individual selection of
the medium components for the most successful multiplication
in vitro. In the article we present the results
of studies on the cultivation of the grapes apexes
in vitro the modified medium with the reduced
content of macrocells. As a result of conducted investigations
we have established that for the grapes varieties
of Academic Trubilin, Artemis, Gurman
Kraynova, Maria Kallas, Nizina, Petit Verdo and
Traminer Black the most effective introduction into
the in vitro culture (acclimatization of apexes 80-
100%) occurs on modified nutrient medium to
Murasige and Skoog (1962), by being differed in
terms of the content macro-elements (mG/l):
NH4NO3 – 1237; KNO3 – 1425; MgSO4 ·
7N2O – 277,5; KN2PO4 · H2O – 277,5, of the
vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l.
Remaining varieties of Kishmish luchistiy, Preobrajenie,
Roshfor K and Yubiley Novocherkasska in the
same medium were developed slowly and in a different
way
This work considers the needs and methods of phage
therapy of two bacterial infections in poultry. The
review examines the use of bacteriophages, from the
moment of their discovery to the present day. We
discuss the relevance of such diseases as
compilobacteriosis and colibacteriosis transmitted
from birds to humans, we show how to use
bacteriophages and their efficacy in models of adult
birds and chickens, including information provided on
the pilot study of phage therapy at a poultry farm. The
reported study was partially supported by RFBR,
research projects No. 16-44-230855 and No. 13-04-
00991 and № 16-44-230855-р_а
The article presents the data on the chemical elements
content in soils, plant leaves and lichens of three
squares in Rostov-on-Don. The calculation of the total
index of soil contamination by chemical elements finds
that soils of the square are characterized by a low
degree of contamination
The article deals with the problem of eliminating
environmental pollution by oil-contaminated waste. It's
suggested modern ways of improving technologies for
oily waste utilization. The comparative characteristic
of the essence, advantages and disadvantages,
effectiveness of ways of waste recycling is described.
The basic utilization technologies of oil sludge of
various origins are emphasized taking into account the
aspects of their composition and properties. It's
represented the results of a patent search in this area to
ensure a high level of new developments. It's
characterized the developed technologies for
utilization and recycling of oily waste to produce
environmentally products of utilization – organic and
mineral additives which are suitable for use as
secondary material resources
An important element in the technology of growing
seed plants of onion is the use of physiologically active
substances to protect plants from frost in early spring
growing season. Damage to plants during this period
affects seed production. In the experiment we used
preparation called Epin-Extra R, with which the leaves
of plants were treated a day before expected frost. Test
results have shown high efficiency of preparation in the
field and in the film unheated greenhouse. Treatment
helps to balance the negative impact of low
temperatures and obtain an additional seeds yield 92-95
kg / ha more compared with the control, due to the
increase of seed of productivity of plants to 3.2 g
The research results on regeneration activity of
wilding cuttings of phylloxeraresistant varieties of
grapes influenced by heteroauxin treatment are
analysed in the article. The preliminary studies were
held in All-Russian research institute of viticulture
and wine-making of Ya.I. Potapenko using cuttings of
3 grape varieties – Berlandieri x Riparia Cober 5bb,
Riparia x Rupestris 101-14 and Berlandieri x Riparia
CO-4. The cuttings were soaked during 48 hours in
0,005 % heteroauxin and were implanted in sand in
adjustable conditions of temperature, humidity and
aeration. In 2012-2013 experiments were continued in
Kuban State Agrarian University. The varieties
Gravesak, RSB and Berlandieri x Riparia Teleki 5C
were added to the experiment. Three-items buds were
shooted in damp steamed sawdust in a film hothouse.
In preliminary researches application of heteroauxin
has allowed to increase root-growing by 20,3-40,0 %.
The greatest increase of root-growing (40 %) was
observed on variety СО-4, that is characterized by the
weakest potential rhizogenesis activity, and the least
(20,3 %) on control variety Cober 5 bb. Next
researches show that treatment of cuttings basal ends
by 0,01 % heteroauxin activated their regeneration
process, that was expressed in: increase in sprouts
length – variety 5 C, 101-14 and Gravesak by 23,1-
157,6 %; increasing of cuttings root-growing from
16,7 % on variety 5C to 60,0 % on variety Cober 5 bb,
the quantity of cuttings with not fewer of 3 roots from
accordingly 13,0 % to 54,7 %; increasing of roots
from 0,7 on variety СО-4 to 2,7 on variety 101-14 or
in 1,7-2,7 times. The application of heteroauxin on
variety RSB did not show positive results, because of
the variety peculiarities