Diagnosis by PCR-RFLP (polymerase chain reaction –
polymorphism of the lengths of restriction fragments)
is the standard analysis of point mutations for the
diagnosis of allelic polymorphism of candidate genes
related with productive qualities of farm animals.
Along the length of the fragments (RFLP) make a
conclusion about the absence or presence of the point
mutation, and homozygosity or heterozygosity of the
individual. The aim of our work was the optimization
of protocols for conducting PCR-RFLP analysis for
genotyping sheep for genes of the growth hormone
gene differential growth factor and gene of calpastatin
Studies were conducted on pure-bred pigs CT and
DM-1. Determine the productivity of meat quality,
thickness and quality of the bacon, the physicochemical
quality of meat, histostructure longissimus
dorsi experienced pigs fattened using dietary
supplements
Studies were conducted on pure-bred pigs CT and
DM-1. An analysis of variance of economically useful
signs and interior indicators pigs using dietary
supplements "Vita selenium" and "Jerusalem
artichoke"
Diseases cause great damage to agriculture, reducing
yields and decreasing its quality. The losses
are up to 20-30% of potential crop yields. Different
types of rust and powdery mildew cause significant
damage to wheat. Thereby, the growing of
varieties tolerant to these diseases is of great importance
at present. It allows solving such problems
as the improvement of stability of grain production
(especially in the epiphytotic time), improvement
of its quality and reduction of prime
cost of the product. Cultivation of resistant varieties
gives the opportunity to avoid a use of crop
protection chemicals and thus, to improve environment.
The researches were carried out in the
laboratory of intensive soft winter wheat breeding
and seed-growing of FSBSI ARRIGC after I.G.
Kalinenko in 2013-2015. The crop was sown in a
bare fallow. 275 samples of soft winter wheat from
the world collection VIR, Turkey (CIMMYT),
new varieties of domestic and foreign breeding,
varieties and constant selection lines of own selection
were the subjects of the study.
Favorable for disease development weather conditions
allowed evaluating collection samples according
to the degree of infection with leaf rust and powdery mildew under natural conditions.
According to a complex resistance to leaf rust and
powdery mildew we have distinguished the following
samples: ‘Nakhodka’, ‘1226/98’, ‘662/99’,
‘1366/08’, ‘Patriarkh’ (Russia); ‘Shestopalivka’(Ukraine);
‘Simonida’ (Serbia); ‘MV 15-04’
(Hungary); ‘Rialto’ (England); ‘Menestrel’
(France); ‘№42 CIMMYT’ (US). Thus, our conducted
study resulted in distinguishing the samples
of soft winter wheat which are resistant to leaf rust
and powdery mildew and are recommended to use
as a source of resistance and tolerance to these
pathogens. The samples with a combined resistance
to both pathogens are of particular interest
The key task of the agriculture in Russia is the further
improvement of grain production. Thus, prevention of
losses of winter wheat yields because of the diseases
remains relevant. The significance of varieties tolerant
to diseases, especially to smut (fungus) is of primary
importance. Therefore, it’s essential to create initial
material for breeding of the varieties tolerant to smut.
According to the results of the trials with artificial
infection with pathogens, among the samples of
winter wheat there have been identified varieties
belonging to different groups of tolerance to smut.
They are a highly sensitive cultivar ‘к-69361’
(Korea), a sensitive variety ‘Izyuminka’ (Russia),
moderately sensitive variety ‘Asket’ (Russia) and
almost tolerant variety ‘1621/03’ (Russia). We studied
the reaction of the hybrids F2 on infection with smut
in different variants of hybridization to obtain stable
progeny. The correlation between the number of
infected plants in parent and hybrid (F2) populations
have been considered. The productive tillering of the
plants has been determined in all variants of the trials;
it was the least one on the plants with 100% of
infected leaves. The hybrids F3 and F 4 have been
distributed according to the degree of their tolerance
to smut. For further work we have selected the lines
with practical resistance to smut (fungus)
There was given a review of the results of the study in
the conditions of sufficient moisture of Central
Ciscaucasia, the influence of planting terms and
presowing seed treatment by the drug called "TMTDplus",
containing Krezatsin growth stimulator in its
composition, on the development of corn hybrids of
different maturity groups of the selection of Krasnodar
Research Institute of Agriculture named after P.P.
Lukyanenko (Ross 199, Ross 299, Krasnodar 382 and
Krasnodar 410) and the All-Russian Research Institute
of Corn (Mashuk 170, Newton, RIC 345 and Eric), as
well as middle- maturity population Rossiyskaya 1.
The studies were conducted in accordance with the
thematic plan of scientific researches of the chair of
crop and forage production of the Stavropol State
Agrarian University. The soil surface of the
experimental station of StavrGAU was presented as
leached black soil. The technology of growing of
maize on the experimental plot corresponds to the
standard one for the present area and cultivar. The
predecessor is winter wheat. Sowing was performed in
three terms. The first (early) sowing term was carried
out at t = + 7 ... +8 ° C. The second (recommended) -
when t = + 10 ... + 12 ° C. The third (later) sowing
time was carried out at t = +15 ° C. The plant density:
early-maturing hybrids – 70 thousand pieces/ha, is
mid-maturing ones – 60 thousand pieces/ha, middleripe
– 50 thousand piece/ha, middle-later ones – 45
thousand pieces/ha. The scheme is single-row, with
spacing of 70 cm. The application of the studied drug
TMTD-plus helped to reduce the growing season of
maize plants for one - four days. Thus, changing the
sowing terms of maize hybrids and populations, we
can largely control the development of plants changing
the length of the growing season to two weeks and
form a harvesting conveyor, thereby reducing the
intensity of field work
There was given the review of the results of the study
in the arid zone of Central Ciscaucasia, the influence
of planting terms and presowing seed treatment by the
drug called "TMTD-plus", containing the growth
stimulator Krezatsin in its composition, on the
development of corn hybrids of different maturity
groups of the selection of Krasnodar Research Institute
of Agriculture named after P.P. Lukyanenko (Ross
199, Ross 299, Krasnodar 382 and Krasnodar 410) and
the All-Russian Research Institute of Corn (Mashuk
170, Newton, RIC 345 and Eric), as well as middlematurity
population Rossiyskaya 1. The studies were
conducted in accordance with the thematic plan of
scientific researches of the chair of crop and forage
production of the Stavropol State Agrarian University.
The soil surface was presented as southern black earth.
The technology of growing of maize on the
experimental plot corresponds to the standard one for
the present area and cultivar. The predecessor is winter
wheat. Sowing was performed in three terms. The first
(early) sowing term was carried out at t = + 7 ... +8 °
C. The second (recommended) - when t = + 10 ... + 12
° C. The third (later) sowing time was carried out at t =
+15 ° C. The plant density: early-maturing hybrids –
70 thousand pieces/ha, is mid-maturing ones – 60
thousand pieces/ha, middle-ripe – 50 thousand
piece/ha, middle-later ones – 45 thousand pieces/ha.
The scheme is single-row, with spacing of 70 cm. The
application of the studied drug TMTD-plus helped to
reduce the growing season of maize plants for one -
two days. Thus, changing the sowing terms of maize
hybrids and populations, we can largely control the
development of plants changing the length of the
growing season to two weeks and form a harvesting
conveyor, thereby reducing the intensity of field work
The research is aimed at studying the compatibility of
different doses of ecoventura (TU 9283-199-
10514645-13) and limestone in the diets of rearing of
ducks of "Blagovarsky" cross and the impact on
productive performance. As a result of scientific and
economic experience obtained data on the dynamics of
live weight in groups of rearing ducks indicate that
ecomentors in combination with limestone have a
growth promoting effect and a positive effect on the
growth of ducklings. Best growth depending on dose
administration of ecoventura and limestone in feed
composition had a rearing ducks III (ecomentors
(1,0%) + limestone (3,0%) by weight of the feed) and
IV experimental groups (ecomentors (1,0%) +
limestone (3,5%) by weight of the feed). The results of
the study measurements (body length, keel length,
breast width, chest girth, chest depth) and indices
stature (massiveness, the meat content, arizonia),
which were higher in males and females when fed with
mixed fodder with the introduction of ecoventura and
limestone in an amount of 1.0 and 3.5% respectively.
Introduction to feed composition of ecoventura and
limestone in an amount of 1.0 and 3.5%, respectively
had a positive impact on exterior indicators of rearing
ducks
The influence of protective coatings on changes in the
biological characteristics of quality, germination, plant
growth and development of the onion crop quantitative
loss of garlic was investigated for the first time. The
objects of research were winter garlic called Tien Shan
and onion of Allan variety. Water solutions of polyvinyl
alcohol PWS-5, paraffin coating Ð , paraffin-cerezin
composition PK-1 were studied as a protective coatings.
Coating onions and chives before planting them
in the ground delay germination, respectively at 1 and
2 weeks. Then, protective coatings act as growth stimulants.
The greatest increase in green mass of garlic for
3 months (almost 2 times) compared to the control (uncoated)
prepared using the hydrophobic coating Ð K-1.
Ð K-1 increases the yield of garlic and onions in 1.5
times, and the seeds of onions in 1.3 times. It is recommended
before planting onions to cover onions the
PK-1 in order to prevent his fall germination and receive
further large bulbs weighing 130-131 g. Carbohydrates,
starch, vitamin C, and life-giving moisture
garlic, it is best stored in a Ð K-1 coated serve after
planting in soil more efficient power plant. As a result,
such plant grows better, develops and gives higher
yields
The article considers one of the types of water erosion
– illuvial process, the redistribution of dispersed
particles in the soil profile due to irrigation. The
proposed description of the process of moving silt
particles in the soil profile is based on the theory of
viscose-plastic continuum motion