Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
AGRIS logo UlrichsWeb logo DOAJ logo
Search by author's name Search by title
Sort by: Date Title Views
145 kb

DETERMINATION OF ACUTE TOXICITY OF ROKSATSIN

abstract 1211607124 issue 121 pp. 1975 – 1984 30.09.2016 ru 262
In this article, the authors have defined the acute toxicity of Roksatsin preparation, which represents a 20 % aqueous solution of polyhexamethylene guanidinehydrochloride (PHMG). The acute toxicity of Roksatsin in the first series of experiments was studied on white mice upon intragastric administration. In the second series of the experiments, Roksatsin was administered subcutaneously to white rats and in the third series of experiments the preparation was administered intracisternally to cows. The results of the first series of experiments showed that Roksatsin by the internal method of appointment is slightly toxic to white mice. In this experiment not been a single case of acute intoxication and death of animals. Experiments to determine the acute toxicity of the preparation for white rats were performed in triplicate. In the first series of experiment, the LD50 was 4.8 ml/kg. It is found, that the calculated coefficients LD50 for other two series were close to the first one: the second - 4.2 ml/kg and the third - 4.5 ml/kg. Therefore, the authors found that the Roksatsin preparation upon subcutaneously administration according GOST 12.1.007-76 is a low-toxic compound (4th class of danger). As a result of the third series of the experiments, it was found that the intracisternally administration of Roksatsin preparation to cows in a dose of 5 ml is not toxic to the animal organism. Intracisternal administration of Roksatsin does not have a significant impact on the morphological and biochemical indices of blood. On this basis, Roksatsin preparation with different routes of administration is low-toxic and it can be recommended for clinical researches
183 kb

EFFICIENCY OF ROKSATSIN IN AEROSOL DISINFECTION OF THE LIVESTOCK BUILDINGS

abstract 1211607125 issue 121 pp. 1985 – 1994 30.09.2016 ru 279
The authors briefly describe the properties of polyhexamethyleneguanidine hydrochloride (PHMG), which refers to a broad-spectrum biocide and has antimicrobial activity against Gram-negative and Gram-positive bacteria, viruses and fungi. PHMG has a deodorizing effect, gives the treated surfaces long bactericidal effect, which can be stored depending on the surface and other external factors from 3 days to 8 months. The authors have presented data about the level of bacterial and fungal contamination of air in the dispensary before and after aerosol treatment of Roksatsin. Bacterial contamination of air dispensary determined via the sedimentation method (Koch Method), which is settling microflora (in air), under gravity, on the surface of a growth medium. For the determination of total bacteria and fungi in 1m3 of air the authors make calculations of total aerobic microbial count (TAMC) according to the formula that was proposed by V.L. Omelyanskii. Bacterial contamination of air was evaluated before disinfection. Accounting quality of aerosol disinfection performed by sedimentation microflora on Petri Dishes through 30, 60 and 120 minutes of exposition. In the analysis of the data the authors defined that Roksatsin as a disinfectant has a negative effect on pathogens, namely significantly reduced the content of pathogenic and conditionally pathogenic bacteria and fungi in the air, so it can be used for preventive and compelled aerosol disinfection of air in the livestock buildings
372 kb

THE RESULTS OF IMPLEMENTATION OF CATTLE EMBRYOS TRANSPLANTATION

abstract 1211607138 issue 121 pp. 2177 – 2211 30.09.2016 ru 427
The results of the analysis of the level of implementation of the method of cattle breeding by embryo transfer in an agricultural company from the Krasnodar region with a developed sector of cattle breeding. We have assessed the level of development of all stages of the method in the household, identified errors in the selection of animals and the increased value of culling donor cows and recipients, reduced duration of using recipient cows and high cost of all phases of the transplant process. We have raised a question of the need to change the criteria of economic evaluation of the results of the method
625 kb

MONITORING OF GLYCEMIA IN COWS FOR THE DETECTION OF PRIMARY METABOLIC DISORDERS IN THE TRANSITION PERIOD

abstract 1211607140 issue 121 pp. 2246 – 2287 30.09.2016 ru 193
The article contains a synthesis of modern views on the problem of ketosis and related metabolic disorders. We have given evidences of the exceptional role of hypoglycemia in the development of progressive metabolic diseases of cows during the transition period. The necessity of implementing a program for critical period with mandatory control of glycemia was substantiated
145 kb

CLINICAL PHARMACOLOGY OF A NEW COMPLEX HEPATOPROTECTIVE PREPARATION

abstract 1191605076 issue 119 pp. 1077 – 1088 31.05.2016 ru 304
This article presents the results of the studies of the influence of a new complex hepatoprotective preparation on the basic system of the body, the mechanism of occurrence and manifestation of its biological effects, the dependence of this action from the components that are parts of the preparation, the dose, as well as the regularity of manifestation of possible side effects. The effect of the different doses of the preparation (1% and 2%) on the average daily weight gain and morphological and biochemical indices of the birds’ blood was studied. The conducted research determined a stimulatory influence of the preparation on the growth, development and safety of broiler chickens. The new hepatoprotector exhibits the properties aimed on revitalizing the erythro- and hematopoiesis and magnification of the cellular immunity against the exogenous influence. The use of the preparation helps to improve liver function and reduce the toxic load on hepatocytes, which manifests an increase in a number of metabolic parameters, such as total protein, glucose, calcium, phosphorus. We have noted an expressed hepatoprotective effect on the enzyme activity of AST and the remission of the cytolytic syndrome of the experimental chickens. Thereby it was found out that the complex hepatoprotective preparation has a pronounced pharmacological activity, providing a significant impact on the energy of the broiler chickens’ growth and their safety, morphological and biochemical indices of the blood and metabolic processes in the body of the bird
145 kb

ETIOLOGICAL ASPECTS OF ANEMIA IN DOG INTOXICATION

abstract 1171603050 issue 117 pp. 773 – 785 31.03.2016 ru 456
Dog poisoning toxicant used at home for rodent control (often based on zinc phosphide ) is accompanied by the development of anemia . In etiopathogenesis of this type of pathology of the blood we can highlight as important: haemolytic , haemorrhagic and allergic components, but the nature and extent of the changes under there are unequal. This requires the development of a special algorithm of examination and treatment strategies of the animal in accordance with the stages of the development of the disease. Modern conditions dictate the need, along with the actions to carry out urgent medical identification as the main etiological factor and pathogenesis, and the leading pathogenetic factors that pose a threat to the danger of other pathological processes and disease states. First, we evaluate the extent of damage to the liver, kidneys, heart, spleen and blood vessels, which is possible only if the clinic has appropriate methods and equipment. It is undeniable in this regard the importance of evidence-based recommendations for dietary nutrition of the affected animal's behavior after the clinic urgent remedial measures. The article proves high importance of evaluating the effectiveness of the treatment in the clinic
165 kb

THE EFFECT OF DALARGIN ON MYELOID LINK OF WHITE RATS' BLOOD SYSTEM WITH EXPERIMENTAL HYPOTHYROIDISM

abstract 1161602006 issue 116 pp. 110 – 119 29.02.2016 ru 647
Under conditions of white rats' hypothyroidism the changes were studied in myeloid link of blood system and corrective action of dalargin. It has been stated that dalargin causes lasting leukopenia, short-time eosinopenia with consequent normalization of their number and momentarily stimulates neutrophilopoiesis
141 kb

APPLICATION OF THE ANK ANOLYTE SUPER NEW GENERATION DRUG FOR DISINFECTION OF HATCHING EGGS

abstract 1081504033 issue 108 pp. 456 – 467 30.04.2015 ru 1397
Spraying the hatching eggs with disinfecting solution was found to be the effective mean to decrease a risk of bacterial contamination. The most used disinfection means contain quaternary ammonium compounds, phenols, iodine of glutaric aldehyde. However, some disinfectants have ability to close pores on to the egg in case of applicating on the surface of egg that results in decreasing evaporation of water during incubation and decreasing hatching. The most popular methods is gazation with using the preparation on a base of formaldehyde, formalin, potassium permanganate. The above-mentioned methods have the risks of potential danger for hatching eggs and developing embryos. For biocidic treatment of eggs YF-irradiation is used, as well as ozone, but it penetrates into eggs owing to eggs pores and destabilizes embryo. Moreover, ozone possesses the toxic, corrosive and inflammable properties and its using must be controlled. Some disinfectants, especially chloral-containing preparation have a property to react with cuticle of eggshell and falls their activity. Other disinfectants, as a rule, block the pores and make difficult gazoexchanging. Using the preparation on a base of electroactivatel solutions of sodium chloride. Was detected to be one of the perspective approaches to disinfection of hatching eggs. The results of study on a development of disinfection technology for hatching eggs contaminated by bacteria and fungi with using the Super ANK Anolyte
135 kb

APPLICATION OF "SUPER ANK ANOLYTE" FOR BIOCIDAL TREATMENT OF EGGS

abstract 1081504034 issue 108 pp. 468 – 478 30.04.2015 ru 1287
Under the field conditions breeding the young poultry takes place in the eggs out of maternal body. For successful breeding the progeny keeping all the demands to safety and quality of hatching eggs has the important significance. Safety demands are presented in the appropriate Technical Standards (TS). The microbial standards are to be very significant. Moreover, it is necessary to control both the microorganisms note in TS and other species as dangerous factors on during the process of production. The methods and means used presently for disinfection of hatching eggs have some disadvantages, e.g. the method of gazation with using the preparations on a base of formaldehyde, formalin, potassium permanganate; ozone has the toxical, corrosive and inflammable properties and its using must be controlled; chlorine-containing disinfectants have a property to react with cuticle of eggshell and fall their activity. The results of a study on applicating Super ANK Anolyte for biocidal treatment of the eggs are presented in the article
149 kb

THE METHOD OF THE MICROBIOLOGICAL ANALYSIS OF THE AIR

abstract 1081504037 issue 108 pp. 500 – 511 30.04.2015 ru 1190
The article represents the characteristics of different methods of air microbiological analysis on the basis of the results of patent searches, the aim of which is to identify and develop the most effective methods for microbiological evaluation of air quality in livestock buildings. This problem has particular relevance in the implementation of anti-epizootic measures. Among the studied methods of air microbiological analysis was used the new method which allows accurate counting degree of bacterial contamination, due to the additional coverage of molten and cooled to 45 ° C in culture medium, the density of which is not less than the density of the main medium. The new method for the microbiological analysis of air was developed and offered for practical application, including the sedimentation of aerosol particles and seeding microorganisms containing in the air at the surface of dense main medium, the temperature control of the samples and the count the microorganisms colonies number
.