Scientific Journal of KubSAU

The "multiplex" PCR technique in a combination with automatic extraction of nucleonic acids allows to carry out the control of activators of sharp intestinal infections of the bacterial and virus nature over objects of an environment with high speed, specificity and sensitivity

Efficiency of correction of hepatic insufficiency of dogs by means of a medicinal solution on a basis of cluster silver and bile is shown. After application of therapy for the ill dogs, it was marked: restoration of the clinical status, morbidity disappearance in belly area and yellowness of mucous membranes for 9 days. For 19 days biochemical indicators of blood reached level of healthy dogs

The influence of the complex alumosilicate prepara-tion of Silimix on productivity, rates of red blood cells and certain microcells in blood serum against nutri-tional anemia of calves is studied. The increase in additional weights of calves and antianemic effect of the preparation is established

The diagnostic preparations created on the basis of polymeric carriers, find wide application in diagnostics of various diseases with the purpose of detection of small quantity of antibodies or antigenes in various substrata. Due to the physical and chemical properties, polymeric particles are convenient carriers at creation of various diagnostic tests - systems in area immunoassay. It is possible to attribute the following moments to advantages of reaction of latex - agglutination: simplicity and speed of performance, absence of necessity for the complex equipment, reproducibility and accuracy, an opportunity of reception of the big parties of standard and homogeneous polymeric suspensions

In this article, the authors have defined the acute toxicity of Roksatsin preparation, which represents a 20 % aqueous solution of polyhexamethylene guanidinehydrochloride (PHMG). The acute toxicity of Roksatsin in the first series of experiments was studied on white mice upon intragastric administration. In the second series of the experiments, Roksatsin was administered subcutaneously to white rats and in the third series of experiments the preparation was administered intracisternally to cows. The results of the first series of experiments showed that Roksatsin by the internal method of appointment is slightly toxic to white mice. In this experiment not been a single case of acute intoxication and death of animals. Experiments to determine the acute toxicity of the preparation for white rats were performed in triplicate. In the first series of experiment, the LD50 was 4.8 ml/kg. It is found, that the calculated coefficients LD50 for other two series were close to the first one: the second - 4.2 ml/kg and the third - 4.5 ml/kg. Therefore, the authors found that the Roksatsin preparation upon subcutaneously administration according GOST 12.1.007-76 is a low-toxic compound (4th class of danger). As a result of the third series of the experiments, it was found that the intracisternally administration of Roksatsin preparation to cows in a dose of 5 ml is not toxic to the animal organism. Intracisternal administration of Roksatsin does not have a significant impact on the morphological and biochemical indices of blood. On this basis, Roksatsin preparation with different routes of administration is low-toxic and it can be recommended for clinical researches

In medical practice, RLA as the alarm express train - test for revealing antigenes of microorganisms is convenient at use in diagnostic laboratories, and also at carrying out of mass inspections. To advantages of RLA, as a method of serologic diagnostics of bacterial and virus infections, it is possible to attribute the following moments: high specificity and sensitivity; absence of necessity for the complex equipment for statement and reactions and registration of results; data up to a minimum of quantity of components of reaction; an opportunity of reception of inert carriers with the set characteristics; an opportunity of reception of inert carriers with the set characteristics

There are numerous reports about the possibility of sensitization to tuberculin animals when infected with pectoris and Rhodococcus and it is considered to be expedient to create one monoallergenov to differentiate allergic reactions. In this context, the spread of pectoris and Rhodococcus in nature, the general physico-chemical and biological properties of mycobacteria, increasingly emerging reports of a possible sensitization of the microorganism, require a detailed study of them in order to determine the specificity of allergy

Study of dynamic sensitive of microorganisms shows wave shape change of characters with change of dominance with sensitive aminoglicozids and hinolone ftorhinolone groups of drugs

Experience on studying of immune genetic properties of esherechious anatoxin is described in the article. There was revealed that new complex of inactivated toxins of intestinal rod is able to stimulate antibodies forming in animal organisms.

The authors briefly describe the properties of polyhexamethyleneguanidine hydrochloride (PHMG), which refers to a broad-spectrum biocide and has antimicrobial activity against Gram-negative and Gram-positive bacteria, viruses and fungi. PHMG has a deodorizing effect, gives the treated surfaces long bactericidal effect, which can be stored depending on the surface and other external factors from 3 days to 8 months. The authors have presented data about the level of bacterial and fungal contamination of air in the dispensary before and after aerosol treatment of Roksatsin. Bacterial contamination of air dispensary determined via the sedimentation method (Koch Method), which is settling microflora (in air), under gravity, on the surface of a growth medium. For the determination of total bacteria and fungi in 1m3 of air the authors make calculations of total aerobic microbial count (TAMC) according to the formula that was proposed by V.L. Omelyanskii. Bacterial contamination of air was evaluated before disinfection. Accounting quality of aerosol disinfection performed by sedimentation microflora on Petri Dishes through 30, 60 and 120 minutes of exposition. In the analysis of the data the authors defined that Roksatsin as a disinfectant has a negative effect on pathogens, namely significantly reduced the content of pathogenic and conditionally pathogenic bacteria and fungi in the air, so it can be used for preventive and compelled aerosol disinfection of air in the livestock buildings