The article represents the characteristics of different methods of air microbiological analysis on the basis of the results of patent searches, the aim of which is to identify and develop the most effective methods for microbiological evaluation of air quality in livestock buildings. This problem has particular relevance in the implementation of anti-epizootic measures. Among the studied methods of air microbiological analysis was used the new method which allows accurate counting degree of bacterial contamination, due to the additional coverage of molten and cooled to 45 ° C in culture medium, the density of which is not less than the density of the main medium. The new method for the microbiological analysis of air was developed and offered for practical application, including the sedimentation of aerosol particles and seeding microorganisms containing in the air at the surface of dense main medium, the temperature control of the samples and the count the microorganisms colonies number
Under conditions of white rats' hypothyroidism the
changes were studied in myeloid link of blood system
and corrective action of dalargin. It has been stated
that dalargin causes lasting leukopenia, short-time
eosinopenia with consequent normalization of their
number and momentarily stimulates neutrophilopoiesis
Dog poisoning toxicant used at home for rodent
control (often based on zinc phosphide ) is
accompanied by the development of anemia . In
etiopathogenesis of this type of pathology of the
blood we can highlight as important: haemolytic ,
haemorrhagic and allergic components, but the
nature and extent of the changes under there are
unequal. This requires the development of a special
algorithm of examination and treatment strategies of
the animal in accordance with the stages of the
development of the disease. Modern conditions
dictate the need, along with the actions to carry out
urgent medical identification as the main etiological
factor and pathogenesis, and the leading
pathogenetic factors that pose a threat to the danger
of other pathological processes and disease states.
First, we evaluate the extent of damage to the liver,
kidneys, heart, spleen and blood vessels, which is
possible only if the clinic has appropriate methods
and equipment. It is undeniable in this regard the
importance of evidence-based recommendations for
dietary nutrition of the affected animal's behavior
after the clinic urgent remedial measures. The article
proves high importance of evaluating the
effectiveness of the treatment in the clinic
This article presents the results of the studies of the
influence of a new complex hepatoprotective preparation
on the basic system of the body, the mechanism
of occurrence and manifestation of its biological effects,
the dependence of this action from the components
that are parts of the preparation, the dose, as well
as the regularity of manifestation of possible side effects.
The effect of the different doses of the preparation
(1% and 2%) on the average daily weight gain
and morphological and biochemical indices of the
birds’ blood was studied. The conducted research determined
a stimulatory influence of the preparation on
the growth, development and safety of broiler chickens.
The new hepatoprotector exhibits the properties
aimed on revitalizing the erythro- and hematopoiesis
and magnification of the cellular immunity against the
exogenous influence.
The use of the preparation helps to improve liver function
and reduce the toxic load on hepatocytes, which
manifests an increase in a number of metabolic parameters,
such as total protein, glucose, calcium,
phosphorus. We have noted an expressed hepatoprotective
effect on the enzyme activity of AST and the
remission of the cytolytic syndrome of the experimental
chickens. Thereby it was found out that the
complex hepatoprotective preparation has a pronounced
pharmacological activity, providing a significant
impact on the energy of the broiler chickens’
growth and their safety, morphological and biochemical
indices of the blood and metabolic processes in the
body of the bird
The article presents the use of intensive methods of
diet herd reproduction that will increase the
productivity and profitability of the sheep industry.
One of such methods is in vitro fertilization (IVF). For
the success of in vitro fertilization procedure, an
important requirement is the availability of highquality
nutrient media, which help to preserve the
genetic material and contribute to the further
development of the zygote. The main requirement to
the media for sperm is the ability of media not to cause
their agglutination. The aim of our work was to search
for new ways to reduce the agglutination of
spermatozoa in the preparation of freshly prepared
sperm in the process of production of embryos in vitro.
To eliminate the agglutination of spermatozoa in the
semen preparation stage, we used GCY medium,
followed by demolition of seed in SOFw environment,
which has resulted in a significant (almost 15 times!)
reduce of the number of bound sperm. In our opinion,
a decrease in agglutination in GCY buffer was
connected with a specific influence of the constituent
components of sperm.
Conclusion: Thus, our method of preparation of
freshly prepared sperm for in vitro fertilization allows
a sharp decline in sperm agglutination, which will
improve the fertility of eggs during the production of
embryos in vitro sheep
The level of development of modern medical
equipment for the past 20-30 years is in constant
development, many of the technologies and methods
of treatment of certain diseases that were previously
only available for the treatment of a person with
success began to penetrate in the veterinary practice.
The objective aim at the initial stage was the
establishment of testing and suitability for the
cultivation of autologous dermal fibroblasts animals of
previously known techniques and the modernization of
the existing techniques. Next, we had to carry out
measurements of morphometric parameters and to
identify structural features and functional activity of
fibroblasts of different kinds of agricultural animals.
Conclusions: The study showed the conducted
morphometric autologous dermal fibroblasts tests
allowed us to obtain the correlation line. In addition, to
identify common patterns in the development and
growth of fibroblasts derived from different species of
agricultural animals. And also, to obtain material for
comparative evaluation of the quality of the obtained
cell cultures using entropy equivalent
According to the title, the article describes the causes and contributing factors of functional disorders of the uterus and ovaries at cows. The authors specified the percentage incidence of these animals’ pathologies in some farms of Krasnodar Region, especially in industrial complexes. Studies have shown that acute postpartum endometritis has a definite influence on the functional disorders of the ovaries at cows. Authors have found that the cows via 2-4 months after calving with ovarian hypofunction in early postnatal period have had purulent-catarrhal endometritis in 87.9%, fibrinous - in 12% of cases. At cows which have had persistent corpus luteum in 54.6% of cases was registered purulent-catarrhal endometritis, in 27.6% - fibrinous, in 17.8% - necrotizing metritis. At cows with ovarian cysts fibrinous endometritis was observed in 63.2% of cases, necrotizing metritis - in 36.7%. Thus, the more severe form of uterine inflammation was observed in cows, the more severe form of ovarian functional disorders observed in them. The authors found that any factors which negatively acting on the central nervous system or hormone levels, directly or indirectly, sharply reduce the reproductive function of animals
ЖИВОТНОВОДСТВО, КРУПНЫЙ РОГАТЫЙ СКОТ, ДИСФУНКЦИЯ ЯИЧНИКОВ, ЭНДОМЕТРИТ, БЕСПЛОДИЕ, СУБИНВОЛЮЦИЯ МАТКИ, ПОЛОВОЙ ЦИКЛ, ПЕРСИСТИРУЮЩЕЕ ЖЕЛТОЕ ТЕЛО, КИСТА ЯИЧНИКОВ, ГОРМОНЫ
In this article, the authors have defined the acute toxicity of Roksatsin preparation, which represents a 20 % aqueous solution of polyhexamethylene guanidinehydrochloride (PHMG). The acute toxicity of Roksatsin in the first series of experiments was studied on white mice upon intragastric administration. In the second series of the experiments, Roksatsin was administered subcutaneously to white rats and in the third series of experiments the preparation was administered intracisternally to cows. The results of the first series of experiments showed that Roksatsin by the internal method of appointment is slightly toxic to white mice. In this experiment not been a single case of acute intoxication and death of animals. Experiments to determine the acute toxicity of the preparation for white rats were performed in triplicate. In the first series of experiment, the LD50 was 4.8 ml/kg. It is found, that the calculated coefficients LD50 for other two series were close to the first one: the second - 4.2 ml/kg and the third - 4.5 ml/kg. Therefore, the authors found that the Roksatsin preparation upon subcutaneously administration according GOST 12.1.007-76 is a low-toxic compound (4th class of danger). As a result of the third series of the experiments, it was found that the intracisternally administration of Roksatsin preparation to cows in a dose of 5 ml is not toxic to the animal organism. Intracisternal administration of Roksatsin does not have a significant impact on the morphological and biochemical indices of blood. On this basis, Roksatsin preparation with different routes of administration is low-toxic and it can be recommended for clinical researches
The authors briefly describe the properties of polyhexamethyleneguanidine hydrochloride (PHMG), which refers to a broad-spectrum biocide and has antimicrobial activity against Gram-negative and Gram-positive bacteria, viruses and fungi. PHMG has a deodorizing effect, gives the treated surfaces long bactericidal effect, which can be stored depending on the surface and other external factors from 3 days to 8 months. The authors have presented data about the level of bacterial and fungal contamination of air in the dispensary before and after aerosol treatment of Roksatsin. Bacterial contamination of air dispensary determined via the sedimentation method (Koch Method), which is settling microflora (in air), under gravity, on the surface of a growth medium. For the determination of total bacteria and fungi in 1m3 of air the authors make calculations of total aerobic microbial count (TAMC) according to the formula that was proposed by V.L. Omelyanskii. Bacterial contamination of air was evaluated before disinfection. Accounting quality of aerosol disinfection performed by sedimentation microflora on Petri Dishes through 30, 60 and 120 minutes of exposition. In the analysis of the data the authors defined that Roksatsin as a disinfectant has a negative effect on pathogens, namely significantly reduced the content of pathogenic and conditionally pathogenic bacteria and fungi in the air, so it can be used for preventive and compelled aerosol disinfection of air in the livestock buildings