Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
AGRIS logo UlrichsWeb logo DOAJ logo
Search by author's name Search by title
Sort by: Date Title Views
149 kb

THE METHOD OF THE MICROBIOLOGICAL ANALYSIS OF THE AIR

abstract 1081504037 issue 108 pp. 500 – 511 30.04.2015 ru 1728
The article represents the characteristics of different methods of air microbiological analysis on the basis of the results of patent searches, the aim of which is to identify and develop the most effective methods for microbiological evaluation of air quality in livestock buildings. This problem has particular relevance in the implementation of anti-epizootic measures. Among the studied methods of air microbiological analysis was used the new method which allows accurate counting degree of bacterial contamination, due to the additional coverage of molten and cooled to 45 ° C in culture medium, the density of which is not less than the density of the main medium. The new method for the microbiological analysis of air was developed and offered for practical application, including the sedimentation of aerosol particles and seeding microorganisms containing in the air at the surface of dense main medium, the temperature control of the samples and the count the microorganisms colonies number
165 kb

THE EFFECT OF DALARGIN ON MYELOID LINK OF WHITE RATS' BLOOD SYSTEM WITH EXPERIMENTAL HYPOTHYROIDISM

abstract 1161602006 issue 116 pp. 110 – 119 29.02.2016 ru 962
Under conditions of white rats' hypothyroidism the changes were studied in myeloid link of blood system and corrective action of dalargin. It has been stated that dalargin causes lasting leukopenia, short-time eosinopenia with consequent normalization of their number and momentarily stimulates neutrophilopoiesis
145 kb

ETIOLOGICAL ASPECTS OF ANEMIA IN DOG INTOXICATION

abstract 1171603050 issue 117 pp. 773 – 785 31.03.2016 ru 786
Dog poisoning toxicant used at home for rodent control (often based on zinc phosphide ) is accompanied by the development of anemia . In etiopathogenesis of this type of pathology of the blood we can highlight as important: haemolytic , haemorrhagic and allergic components, but the nature and extent of the changes under there are unequal. This requires the development of a special algorithm of examination and treatment strategies of the animal in accordance with the stages of the development of the disease. Modern conditions dictate the need, along with the actions to carry out urgent medical identification as the main etiological factor and pathogenesis, and the leading pathogenetic factors that pose a threat to the danger of other pathological processes and disease states. First, we evaluate the extent of damage to the liver, kidneys, heart, spleen and blood vessels, which is possible only if the clinic has appropriate methods and equipment. It is undeniable in this regard the importance of evidence-based recommendations for dietary nutrition of the affected animal's behavior after the clinic urgent remedial measures. The article proves high importance of evaluating the effectiveness of the treatment in the clinic
145 kb

CLINICAL PHARMACOLOGY OF A NEW COMPLEX HEPATOPROTECTIVE PREPARATION

abstract 1191605076 issue 119 pp. 1077 – 1088 31.05.2016 ru 718
This article presents the results of the studies of the influence of a new complex hepatoprotective preparation on the basic system of the body, the mechanism of occurrence and manifestation of its biological effects, the dependence of this action from the components that are parts of the preparation, the dose, as well as the regularity of manifestation of possible side effects. The effect of the different doses of the preparation (1% and 2%) on the average daily weight gain and morphological and biochemical indices of the birds’ blood was studied. The conducted research determined a stimulatory influence of the preparation on the growth, development and safety of broiler chickens. The new hepatoprotector exhibits the properties aimed on revitalizing the erythro- and hematopoiesis and magnification of the cellular immunity against the exogenous influence. The use of the preparation helps to improve liver function and reduce the toxic load on hepatocytes, which manifests an increase in a number of metabolic parameters, such as total protein, glucose, calcium, phosphorus. We have noted an expressed hepatoprotective effect on the enzyme activity of AST and the remission of the cytolytic syndrome of the experimental chickens. Thereby it was found out that the complex hepatoprotective preparation has a pronounced pharmacological activity, providing a significant impact on the energy of the broiler chickens’ growth and their safety, morphological and biochemical indices of the blood and metabolic processes in the body of the bird
141 kb

COMPARATIVE CHARACTERISTICS OF SPERM FUNCTIONAL PARAMETERS OF SHEEP NORTH CAUCASIAN BREED WHEN YOU MAKE IT TO THE MEDIA FOR IN VITRO FERTILIZATION

abstract 1211607054 issue 121 pp. 907 – 916 30.09.2016 ru 724
The article presents the use of intensive methods of diet herd reproduction that will increase the productivity and profitability of the sheep industry. One of such methods is in vitro fertilization (IVF). For the success of in vitro fertilization procedure, an important requirement is the availability of highquality nutrient media, which help to preserve the genetic material and contribute to the further development of the zygote. The main requirement to the media for sperm is the ability of media not to cause their agglutination. The aim of our work was to search for new ways to reduce the agglutination of spermatozoa in the preparation of freshly prepared sperm in the process of production of embryos in vitro. To eliminate the agglutination of spermatozoa in the semen preparation stage, we used GCY medium, followed by demolition of seed in SOFw environment, which has resulted in a significant (almost 15 times!) reduce of the number of bound sperm. In our opinion, a decrease in agglutination in GCY buffer was connected with a specific influence of the constituent components of sperm. Conclusion: Thus, our method of preparation of freshly prepared sperm for in vitro fertilization allows a sharp decline in sperm agglutination, which will improve the fertility of eggs during the production of embryos in vitro sheep
557 kb

MORPHOLOGICAL CHARACTERISTICS OF AUTOLOGOUS DERMAL FIBROBLASTS OF DIFFERENT KINDS OF AGRICULTURAL ANIMALS

abstract 1211607055 issue 121 pp. 917 – 928 30.09.2016 ru 680
The level of development of modern medical equipment for the past 20-30 years is in constant development, many of the technologies and methods of treatment of certain diseases that were previously only available for the treatment of a person with success began to penetrate in the veterinary practice. The objective aim at the initial stage was the establishment of testing and suitability for the cultivation of autologous dermal fibroblasts animals of previously known techniques and the modernization of the existing techniques. Next, we had to carry out measurements of morphometric parameters and to identify structural features and functional activity of fibroblasts of different kinds of agricultural animals. Conclusions: The study showed the conducted morphometric autologous dermal fibroblasts tests allowed us to obtain the correlation line. In addition, to identify common patterns in the development and growth of fibroblasts derived from different species of agricultural animals. And also, to obtain material for comparative evaluation of the quality of the obtained cell cultures using entropy equivalent
139 kb

THE CAUSAL AND INVESTIGATORY FACTORS OF FUNCTIONAL DISORDERS OF UTERUS AND OVARIES AT COWS ON INDUSTRIAL FARMS

abstract 1211607112 issue 121 pp. 1809 – 1817 30.09.2016 ru 722
According to the title, the article describes the causes and contributing factors of functional disorders of the uterus and ovaries at cows. The authors specified the percentage incidence of these animals’ pathologies in some farms of Krasnodar Region, especially in industrial complexes. Studies have shown that acute postpartum endometritis has a definite influence on the functional disorders of the ovaries at cows. Authors have found that the cows via 2-4 months after calving with ovarian hypofunction in early postnatal period have had purulent-catarrhal endometritis in 87.9%, fibrinous - in 12% of cases. At cows which have had persistent corpus luteum in 54.6% of cases was registered purulent-catarrhal endometritis, in 27.6% - fibrinous, in 17.8% - necrotizing metritis. At cows with ovarian cysts fibrinous endometritis was observed in 63.2% of cases, necrotizing metritis - in 36.7%. Thus, the more severe form of uterine inflammation was observed in cows, the more severe form of ovarian functional disorders observed in them. The authors found that any factors which negatively acting on the central nervous system or hormone levels, directly or indirectly, sharply reduce the reproductive function of animals
186 kb

FACTORS CONTRIBUTING TO THE EMERGENCE OF FUNCTIONAL DISORDERS OF BIRTH-GENITAL APPARATUS AT COWS

abstract 1211607113 issue 121 pp. 1818 – 1827 30.09.2016 ru 519
ЖИВОТНОВОДСТВО, КРУПНЫЙ РОГАТЫЙ СКОТ, ДИСФУНКЦИЯ ЯИЧНИКОВ, ЭНДОМЕТРИТ, БЕСПЛОДИЕ, СУБИНВОЛЮЦИЯ МАТКИ, ПОЛОВОЙ ЦИКЛ, ПЕРСИСТИРУЮЩЕЕ ЖЕЛТОЕ ТЕЛО, КИСТА ЯИЧНИКОВ, ГОРМОНЫ
145 kb

DETERMINATION OF ACUTE TOXICITY OF ROKSATSIN

abstract 1211607124 issue 121 pp. 1975 – 1984 30.09.2016 ru 647
In this article, the authors have defined the acute toxicity of Roksatsin preparation, which represents a 20 % aqueous solution of polyhexamethylene guanidinehydrochloride (PHMG). The acute toxicity of Roksatsin in the first series of experiments was studied on white mice upon intragastric administration. In the second series of the experiments, Roksatsin was administered subcutaneously to white rats and in the third series of experiments the preparation was administered intracisternally to cows. The results of the first series of experiments showed that Roksatsin by the internal method of appointment is slightly toxic to white mice. In this experiment not been a single case of acute intoxication and death of animals. Experiments to determine the acute toxicity of the preparation for white rats were performed in triplicate. In the first series of experiment, the LD50 was 4.8 ml/kg. It is found, that the calculated coefficients LD50 for other two series were close to the first one: the second - 4.2 ml/kg and the third - 4.5 ml/kg. Therefore, the authors found that the Roksatsin preparation upon subcutaneously administration according GOST 12.1.007-76 is a low-toxic compound (4th class of danger). As a result of the third series of the experiments, it was found that the intracisternally administration of Roksatsin preparation to cows in a dose of 5 ml is not toxic to the animal organism. Intracisternal administration of Roksatsin does not have a significant impact on the morphological and biochemical indices of blood. On this basis, Roksatsin preparation with different routes of administration is low-toxic and it can be recommended for clinical researches
183 kb

EFFICIENCY OF ROKSATSIN IN AEROSOL DISINFECTION OF THE LIVESTOCK BUILDINGS

abstract 1211607125 issue 121 pp. 1985 – 1994 30.09.2016 ru 637
The authors briefly describe the properties of polyhexamethyleneguanidine hydrochloride (PHMG), which refers to a broad-spectrum biocide and has antimicrobial activity against Gram-negative and Gram-positive bacteria, viruses and fungi. PHMG has a deodorizing effect, gives the treated surfaces long bactericidal effect, which can be stored depending on the surface and other external factors from 3 days to 8 months. The authors have presented data about the level of bacterial and fungal contamination of air in the dispensary before and after aerosol treatment of Roksatsin. Bacterial contamination of air dispensary determined via the sedimentation method (Koch Method), which is settling microflora (in air), under gravity, on the surface of a growth medium. For the determination of total bacteria and fungi in 1m3 of air the authors make calculations of total aerobic microbial count (TAMC) according to the formula that was proposed by V.L. Omelyanskii. Bacterial contamination of air was evaluated before disinfection. Accounting quality of aerosol disinfection performed by sedimentation microflora on Petri Dishes through 30, 60 and 120 minutes of exposition. In the analysis of the data the authors defined that Roksatsin as a disinfectant has a negative effect on pathogens, namely significantly reduced the content of pathogenic and conditionally pathogenic bacteria and fungi in the air, so it can be used for preventive and compelled aerosol disinfection of air in the livestock buildings
.