Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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429 kb

A MODIFIED PROTOCOL OF RNA ISOLATION FROM MATURE LEAVES OF GRAPES FOR RT-PCR

abstract 1431809012 issue 143 pp. 16 – 30 30.11.2018 ru 196
Isolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
255 kb

AGRIBIOLOGICAL AND TECHNOLOGICAL INDICATORS CHANGES DESCRIPTION OF VIORIKA GRADE GRAPE UNDER THE TREATMENT OF FARMAIODINE

abstract 1401806023 issue 140 pp. 73 – 99 29.06.2018 ru 48
The results of the researches on studying of grape plantings processing influence of technical grade Viorika on its agrobiological and technological indicators are stated in the article. Experiences have been conducted in public joint-stock company "Pobeda" in Temrjuksky area in Krasnodar territory. The experience scheme included three variants in 2015: without processings (control); Farmaiodne - 0,04 %; Farmaiodne - 0,06 %; in 2016 - five variants: without processings (control); Farmaiodne - 0,04 %; Farmaiodne - 0,05 %; Farmaiodne - 0,06 %; Farmaiodne - 0,08 %. Processing of experimented plants included autumn and spring bushes washing in resting period (November-April) and spraying during the vegetation period. In 2015 spraying were conducted three times: before flowering, in a growth phase of berries, in the beginning of berries maturing phase; in 2016 the fourth spraying was added after the flowering. It is noticed, that application of Farmaiodne promotes more intensive accumulation of sugars in berries juice, acceleration of crop maturing, improvement of winematerials quality. The increasing of sprouts fruitfulness and increasing in average weight of a bunch is observed, that finally leads to authentic crop increasing in a bush and productivity. Application of Farmaiodne also has positive impact on growing processes, promoting increase in sprouts length and sometimes on thickness. According to the mixture of agrobiological and technological indicators the best results are received at the application in concentration of 0,05 % and 0,06%
348 kb

AGROBIOLOGICAL AND ECONOMIC FEASIBILITY OF CONVEYOR PRODUCTION OF WATERMELON IN THE "IP EROKHINA E. A." COMPANY IN THE TEMRYUK DISTRICT

abstract 1381804017 issue 138 pp. 78 – 90 30.04.2018 ru 179
Watermelon has great economic importance. The fruits have high nutritional and medicinal value, excellent taste, very healthy and are in great demand among the population. Soil and climatic conditions of the Temryuk district are favourable for growing watermelon. High yield and excellent fruit quality in the commercial led to the popularity of the Temryuk watermelon not only on the black sea coast, but also throughout Russia. The urgency of improving the assortment of the watermelon is increased competition among manufacturers, increasing consumer culture of the population, the increase in sales volumes, including due to the appearance on the market of varieties and hybrids of non-traditional colors of bark and pulp. The aim of our study was to establish the most adapted hybrids of watermelon of different segments, with high yield and marketability of fruits, which are promising for growing in the Temryuk district. Scientific novelty of our work lead to the research objects – new, recently created hybrids of watermelon, promising for cultivation in the Krasnodar region. The work has great practical significance, since on the basis of these studies identified promising hybrids of watermelon foreign seeds of various segments of the precocity, which with appropriate cultivation agrobiological and economic points of view
132 kb

AGROBIOLOGICAL EVALUATION OF NEW STRAWBERRY VARIETIES IN THE CONDITIONS OF THE PRIKUBANSKAYA ZONE OF GARDENING IN THE KRASNODAR REGION

abstract 1491905016 issue 149 pp. 0 – 0 31.05.2019 ru 27
The article presents the results of studies on the biological characteristics of growth and fruiting, as well as the yield of adaptive varieties of strawberries in the conditions of the Prikubanskaya horticulture zone of the Krasnodar region. The objects of study are new and promising varieties of strawberries: Zenga-zengana (k), Onda, San-Diaz. The experiments were performed using the method of randomized reps. In the experiment there are 3 options, in each there are three replications of 20 plants. Variants and repetitions are placed sequentially. Agricultural records of the growth and fruiting of strawberry plants were carried out according to generally accepted methods; statistical processing of research results was carried out using the analysis of a variance method described by B.A.Dospehov. The results of our research showed that Zenga-zengana bushes (control) turned out to be the tallest. In the Onda variety, the height of the bush is 2 cm less than in the control variety; and San Diez varieties are 4 cm, respectively. It should be noted that the most undersized plants require a smaller area of nutrition. It has been established that flowers pollinated a few days after their disclosure develop more rapidly into berries than those that were pollinated immediately after opening. Pollen in flowers of the first order is less than in flowers of the third or fourth order. The stamens of the first flowers are often underdeveloped, their pollen is released and germinates worse. In our experience, the average weight of fruits and the yield as a whole depends on the biological productivity of the varieties. Onda was the most fruitful variety, and the rest varieties were at the control level
129 kb

ANALYSIS OF TECHNOLOGIES FOR THE PRODUCTION OF FUEL PELLETS BASED ON RAW WOOD

abstract 1491905018 issue 149 pp. 0 – 0 31.05.2019 ru 33
The article presents a brief overview of the technologies for the production of wood-based pellets. The work gives disadvantages and advantages of the products manufactured using the surveyed technologies. A comparative analysis of the granules obtained by the considered technological processes is given. The conclusions are made about the economic and technological feasibility of using the presented technologies using wood waste as a raw material
336 kb

ANATOMICAL AND MORPHOLOGICAL COMPARISON OF PEPPERMINT (MENTHA PIPERITA)

abstract 1401806013 issue 140 pp. 24 – 33 29.06.2018 ru 1057
The article presents experimental data of comparing anatomical and morphological features and properties of two peppermint varieties in the Hyaginsky district of the Adygea Republic. The economic importance of peppermint is very high. The essential oil rich in menthol, obtained from the leaves and mint colors, is widely used in the medical, perfumery, cosmetic and food industries. In this regard, of great value are varieties of a complex purpose - with high leaf productivity and high % essential oil and menthol. Comparative analysis of morphological and anatomical features of peppermint raw material of peppery two varieties harvested in the territory of the Farm Sazonenko NV of Giaginsky district of the Adygea Republic, showed that the external features of plants differ from each other. The Kubanskaya 6 variety leaves have a larger size, with a higher content of essential oils, incl. menthol. In this regard, the yield of this variety was higher than that of Prilukskaya 6, and therefore the economic indicators of cultivation of the Kubanskaya 6 variety were the best
177 kb

ASSESSMENT OF THE IMPACT OF MAN-MADE OBJECTS ON THE SPECIFIC OF HEAVY METALS ACCUMULATION IN THE AGRICULTURAL LANDSCAPE

abstract 1471903017 issue 147 pp. 0 – 0 29.03.2019 ru 30
As a result of the researching of anthropogenic influence on the agricultural landscape of Public Corporation "Agrofirma "Niva" from human economic activities with are related to: the pesticides and mineral fertilizers using; the work of agricultural machinery, and the movement of vehicles on the highway. The experimental material on the formation of dust masses and the accumulation of heavy metals in the soil. On the researching territory was found out three times excess of zinc MPC* (23 mg/kg, including background) and four times more of lead MPC (6,0 mg/kg, including background) *maximum permissible concentration
246 kb

ASSESSMENT OF THREATS TO THE VASCULAR PLANT DIVERSITY OF NATURAL COMMUNITIES AND OF REINEKE ISLAND FROM THE ALIEN SPECIES CENTAUREA JACEA L. (ASTERAСЕАЕ) (VLADIVOSTOK, PRIMORSKII KRAI, PETER THE GREAT BAY, SEA OF JAPAN)

abstract 1471903011 issue 147 pp. 0 – 0 29.03.2019 ru 15
In Reineke Island (Primorskii Krai, Vladivostok, Peter the Great Bay, Sea of Japan) alien species for Russia’s Far East Centaurea jacea L. (Asteraсеае) were first discovered in the 1980th in a small amount. Now, 40 years later distribution of C. jacea is limited to small sites of secondary grass meadows and disturbed habitats of roadsides unpaved roads for approximately 0.03% of the island; the level of its invasive activity is low; C. jacea threats currently is not for the biodiversity of plant community and forage value of meadows
438 kb

ASSESSMENT OF WEATHER CHANGES EFFECT ON PEACH CROP IN HUMID SUBTROPICS OF RUSSIA

abstract 1481904014 issue 148 pp. 0 – 0 30.04.2019 ru 16
The close dependence of peach yield on the weather factors in the humid subtropical zone has been established. The observed climate changes in recent years (2000–2018) significantly affected the production potential of peach cultivars. An increase in temperature and precipitation in February-March negatively affected morphogenesis of the flower buds, which reduces the yield of most cultivars. The significant variability of precipitation (the main constraint of peach production) in the winter and early spring, the low weather predictability, characterize the onset of flowering by the “critical” phase. Weather conditions were changed during ripening. In July and August, the amount of precipitation decreased, the temperature increased, which led to moisture deficit and fruits quality deterioration. Local climate change in humid subtropics actualizes the development of adaptive cultivars with a high ecological and adaptive plasticity. Selected clones of the Red Heaven, Krasnaya Zarya and Larisa are the most adapted to the local conditions and can be successfully used in the low mountain area of the subtropics
33012 kb

AUTOMATED SYSTEM-COGNITIVE ANALYSIS IN AGRONOMY

abstract 1361802011 issue 136 pp. 87 – 145 28.02.2018 ru 296
Agronomy systems with good reason can be considered as complex multiparameter natural and technical systems. In these systems, there are numerous and diverse physical, chemical and biological processes. On the one hand, these processes have a significant impact on the performance of these systems. On the other hand, they are extremely difficult to be described in the form of meaningful analytical models based on equations. As a result, the development of meaningful analytical models is associated with a large number of simplifying assumptions that reduce the validity of these models. Usually we consider linear univariate models for agronomic systems, whereas practices are necessary for nonlinear multiparameter models. Thus, we face the problem proposed to be solved by the application of a phenomenological meaningful systemic cognitive models. These models are created using automated system-cognitive analysis (ASC-analysis) using the intellectual system called "Eidos" directly based on empirical data and used for the decision of tasks of forecasting, decision support and research of the modeled subject area. In this case, empirical data can be large, incomplete (fragmented), noisy, presented in different types of measuring scales (nominal, ordinal and numerical) and in different units of measurement. The comparability of the processing of heterogeneous data is ensured by the fact that they are all converted into units of measurement of the amount of information. A numerical example has been given
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