The article presents a factorial model for determining the needs of lactating cows in essential amino acids. The algorithm of the model uses fragments from the NRC – 2001 models [39], CNCPS – 200 [60], and research materials published in the world literature. Instead of the transformation coefficients of the metabolizable lysine and methionine for milk production equal to 0.85 and 1.00, respectively, by the CNCPS, the coefficients 0.68 and 0.66 were used according to Doepel et al., 2004 [49] and the authors' own data [69]. Norms obtained using this model in lysine and methionine in milk production and maintenance in percentage of metabolizable protein (MP) were 7.28 and 2.4%, which is identical to the NRC – 2001 standards, equal to 7.2 and 2.4 % respectively, obtained by dose – response method based on dozens of experiments. The norm of histidine was 3.5%, which closely corresponds to the indicator of 2.4 and 2.7 % MP [74], obtained by the incremental addition method. This indicates that the presented model is distinguished by sufficiently high accuracy and is comparable with the models developed by the dose – response method. However, the determination of the need of cows for amino acids in this model is much less expensive than the dose – response method. The need for the absolute amount of metabolizable essential amino acids (MEAA) for milk production (35 kg/d, yield milk protein 1103 gytt6) and maintenance cow - 600 kg, g/d: lysine - 178, methionine - 59, arginine - 119, histidine - 60, isoleucine - 138, leucine - 248, phenylalanine - 152, threonine - 134, tryptophan - 38, valine - 174; the need for only milk production, g/d: 130; 42; 81; 42; 95; 175; 98; 74; 25; 112; the need for only maintenance, g/d: 50; 16; 38; 20; 43; 73; 54; 60; 14; 64; the need for 1 kg of milk (31.5 g of protein) g/kg: 3.7; 1.2; 2.3; 1.2; 2.7; 5.0; 2.8; 2.1; 0.7; 3.2; need to maintain, g / kg 0.75: 0.41; 0.14; 0.31; 0.16; 0.35; 0.60; 0.44; 0.50; 0.11; 0.53. In addition, the article presents the norms of amino acids per 1 kg of dry matter (DM) ration. However, the proposed standards need a comprehensive assessment in the research and practice of dairy farming. Research is needed to improve the models and predict the MEAA in the rations in the following areas: studying the metabolism of amino acids in the body of cows and determining their costs for maintaining the digestive tract, tissues and organs, refining, on this basis, the utilization of amino acids for milk protein production and maintenance; the development of ideal amino-acid profile of low-protein diets, both due to the adjustment of natural ingredients, and through the use of amino acid preparations that are protected from disintegration in the rumen
The article deals with angiogenesis of squamous papillomas of dogs, which is a process of formation of blood vessels, providing neovascularization of tumors with their progression. The differentiation of papillomas depending on morphological and histological features was carried out, progressive and hyperkeratinized types were identified, which determine the stage of development and transition from one to another in the process of tumor growth. It is noted that with the progression of papillomas, the area of the constituent elements of the tumor increases, both due to the stroma and due to the parenchyma, but the stromal-parenchymal ratio changes in the direction of increasing the parenchymal component. Analysis of angiogenesis showed heterogeneity of the isolated vessels, both within the stromal component of papillomas, and depending on the type of tumors. There are three types of vessels – small, medium and large, there is a similar ratio of different types of vessels, regardless of the type of papillomas. Localization of vessels in the stroma depending on belonging to the selected species was studied. The total area of the section of blood vessels with cancer increases, and the ratio of the total area of the section of blood vessels to the area of the stroma is almost at the same level, which determines the dependence of the increase in the stromal component of papillomas from angiogenesis
Smallpox of turkey is a viral, slowly extending disease. It pathogenetically proceeds in a dermatotropic way, skin lesions are characterized primarily by two signs or focal necrotic focus centers or diphtheritic inflammation of mucous membranes, often of the upper respiratory tract. Etiological factor in smallpox are dermatotropic epitheliogenic viruses, the family of Avipoxvirus of Poxviridae. The purpose of this work was studying the features of clinic and the pathomorphological changes at smallpox at turkeys in the context of diagnostics methods and also generalization of treatment-and-prophylactic actions at this pathology. The diseased bird is the main source of infection. In addition, the smallpox virus particles can be spread by people providing care for the birds, indoor air, food, bedding material, excrement. Diagnosis of smallpox takes into account the symptoms; if an autopsy was performed, the result of a pathological pattern as well. Additionally, we can conduct a research of histological sections. Confirmation of diagnosis can be performed by a virological study and the blood serum. In accordance with the pathogenesis of smallpox, there is a classic skin form and diphtheritic. There may be a combination of the above manifestations of smallpox, therefore we can record the mixed form, in some cases, it may be atypical. Pathoanatomical changes come down to defeat a respiratory system of bodies, the upper airways, at the complicated forms there may be noted lesions in the internal organs. Laboratory methods include identification of the virus in the pathological material, as well as additional histological and virological studies using chicken embryos and bioassays. Treatment is difficult and not always effective. The main preventive measure against smallpox in turkey is vaccination, basic applied biologics are: Avivac-pox" strain "K", "Ospovat, Vectorman FP-MG". Prevention of smallpox includes the implementation of general veterinary and sanitary rules in conjunction with the mandatory special prevention. When planning measures to prevent the spread of smallpox, conditions are created to eliminate pathways of transmission of the pathogen – disinfection, disinsection and deratization, as well as vaccination of all susceptible livestock
The article discusses the impact of color lighting on changes in productivity and environmental safety of poultry products. We have given recommendations to optimize the cost of electricity for lighting the house
This prospective study was conducted on 10 commercial dairy herds, over one year on milk urea monitoring, determination of diets characteristics effects on MU concentration and on assessment of MU concentrations as a predictor of N utilization and urinary N excretion. Milk samples were collected twice every month and analyzed for urea concentration using a colorimetric procedure. Representative feed samples were also collected on the same day of milk collection. Feed samples were characterized and their concentrations of protein digestible in the intestine and net energy for lactation were calculated according to the French system as well as PDI requirements. Average of milk urea concentrations range is 25.0 - 32.0 mg/dl. A significant positive association (p
Providing the population with basic food products, sources of proteins, fats, carbohydrates, vitamins and other essential nutrients is one of the main issues of ensuring the security of the country, while the requirement for food safety is paramount. Production of feed additives (concentrates), which improve feed conversion, while being natural and safe for animal health, acquires the greatest importance in the conditions of intensification of agriculture and increasing the competitiveness of livestock farms. Probiotic microflora, which, in competition with pathogenic, displaces the latter from the gastrointestinal tract, can be considered as one of the effective methods of controlling undesirable microflora in the digestive tract. In world science, research is actively conducted in the direction of searching for effective probiotic cultures. A promising way to increase the effectiveness of the use of probiotic feed additives is their addition to prebiotics, for example, including vitamins (antioxidants) and mineral substances (selenium). The purpose of this work was to determine the optimal ratio of biologically active substances contained in the feed vitamin-mineral concentrate, and probiotic microflora contained in the feed probiotic additive Bacell-M, for the development of complex feed concentrate. Using the Harrington's desirability function, the optimum ratio of biologically active substances and probiotic microflora in the recipe of the complex feed concentrate is determined, ensuring the maximum reduction in the serum levels of alanine aminotransferase in the blood serum of experimental animals, which characterizes the normalization of barrier organs
The problems of creating an optimal microclimate in the closed workshops of poultry farms are relevant for the regions of the south of Russia and the Northern Caucasus, especially acute these problems are for Kabardino-Balkaria. The used zoohygienic measures and existing ventilation systems of closed poultry houses need to be improved and adapted to the local natural conditions. The purpose of the work was to research and develop effective ventilation systems for closed poultry houses in hot climates. Existing ventilation systems and zoohygienic conditions in closed-type poultry houses were studied and investigated. According to the results, the main disadvantages of the existing ventilation systems and their low efficiency were established. And on their basis, new technical solutions have been found to create more efficient ventilation systems for closed poultry houses that provide the optimal microclimate: temperature, humidity and pressure. With the active participation of the author, new technical solutions have been developed and proposed, for which two applications for obtaining patents have been prepared: one for a utility model - for a device for moistening and cooling wall ventilation ducts of poultry houses; the second is for the invention - on the method of erecting a cooling system and humidifying the ventilation panels of the supply. During their implementation, these new technical solutions allow creating such a microclimate, thanks to which optimal indicators of productivity and growth, uniformity, efficiency of feed and meat gain are achieved, without reducing the characteristics of the health and well-being of birds. Ventilation is becoming a major factor in microclimate control in closed houses and the epizootological resistance of birds
In this work, we have found shortened particle forms of the filamentous bacteriophage Pf, when the lysis of the Pseudomonas aeruginosa PAO1 strain was induced by extracts of bison feces to study the intestinal bacteriophages of this animal. The sizes of the shortened particles from 250 to 950 nm and their thickness 17 nm were determined. The particles had a slightly pointed one end and sometimes a flag on the other extended end. The particles were formed in the process lysis of this strain by other bacteriophages. These lysates also contained a high concentration of vesicles from the pseudomonad membrane. The article discusses the mechanisms of prophage induction in the Pseudomonas aeruginosa PAO1 strain due to phage-phage induction and substances of the investigated extract
Providing the population with basic foodstuffs, sources of proteins, fats, carbohydrates, vitamins and other essential nutrients is one of the main issues of ensuring the security of the country, while the requirement for food safety is paramount. Production of feed additives (concentrates), which improve feed conversion, while being natural and safe for animal health, acquires the greatest importance in the conditions of intensification of agriculture and increasing the competitiveness of livestock farms. Probiotic microflora, which, in competition with pathogenic, displaces the latter from the gastrointestinal tract, can be considered as one of the effective methods of controlling undesirable microflora in the digestive tract. In world science, research is actively conducted in the direction of searching for effective probiotic cultures. A promising way to increase the effectiveness of the use of probiotic feed additives is their addition to prebiotics, for example, including vitamins (antioxidants) and mineral substances (selenium). The purpose of this work was to determine the optimal ratio of biologically active substances contained in the feed vitamin-mineral concentrate, and probiotic microflora contained in the feed probiotic additive Bacell-M, for the development of complex feed concentrate. Using the Harrington's desirability function, the optimum ratio of biologically active substances and probiotic microflora in the recipe of the complex feed concentrate is determined, ensuring the maximum reduction in the serum levels of alanine aminotransferase in the blood serum of experimental animals, which characterizes the normalization of barrier organs
The article presents a factorial model for determining the needs of lactating cows in essential amino acids. The algorithm of the model uses fragments from the NRC – 2001 models [39], CNCPS – 200 [60], and research materials published in the world literature. Instead of the transformation coefficients of the metabolizable lysine and methionine for milk production equal to 0.85 and 1.00, respectively, by the CNCPS, the coefficients 0.68 and 0.66 were used according to Doepel et al., 2004 [49] and the authors' own data [69]. Norms obtained using this model in lysine and methionine in milk production and maintenance in percentage of metabolizable protein (MP) were 7.28 and 2.4%, which is identical to the NRC – 2001 standards, equal to 7.2 and 2.4 % respectively, obtained by dose – response method based on dozens of experiments. The norm of histidine was 3.5%, which closely corresponds to the indicator of 2.4 and 2.7 % MP [74], obtained by the incremental addition method. This indicates that the presented model is distinguished by sufficiently high accuracy and is comparable with the models developed by the dose – response method. However, the determination of the need of cows for amino acids in this model is much less expensive than the dose – response method. The need for the absolute amount of metabolizable essential amino acids (MEAA) for milk production (35 kg/d, yield milk protein 1103 gytt6) and maintenance cow - 600 kg, g/d: lysine - 178, methionine - 59, arginine - 119, histidine - 60, isoleucine - 138, leucine - 248, phenylalanine - 152, threonine - 134, tryptophan - 38, valine - 174; the need for only milk production, g/d: 130; 42; 81; 42; 95; 175; 98; 74; 25; 112; the need for only maintenance, g/d: 50; 16; 38; 20; 43; 73; 54; 60; 14; 64; the need for 1 kg of milk (31.5 g of protein) g/kg: 3.7; 1.2; 2.3; 1.2; 2.7; 5.0; 2.8; 2.1; 0.7; 3.2; need to maintain, g / kg 0.75: 0.41; 0.14; 0.31; 0.16; 0.35; 0.60; 0.44; 0.50; 0.11; 0.53. In addition, the article presents the norms of amino acids per 1 kg of dry matter (DM) ration. However, the proposed standards need a comprehensive assessment in the research and practice of dairy farming. Research is needed to improve the models and predict the MEAA in the rations in the following areas: studying the metabolism of amino acids in the body of cows and determining their costs for maintaining the digestive tract, tissues and organs, refining, on this basis, the utilization of amino acids for milk protein production and maintenance; the development of ideal amino-acid profile of low-protein diets, both due to the adjustment of natural ingredients, and through the use of amino acid preparations that are protected from disintegration in the rumen